Type of document : Magazine article Edition cipher : Author(s) : Mushtag M., Kovalevska L., Klein G., Kashuba E. Title : High expression levels of MRPS 18-2 and presence of the RB protein are required for the maintenance of stem cell phenotype Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 92-93 (Cipher ЕУ12/2017/39/1) MeSH-main: СТВОЛОВЫЕ КЛЕТКИ -- STEM CELLS БЕЛКИ -- PROTEINS Annotation: We have found that mitochondrial ribosomal protein MRPS18-2 (S18-2) is involved in regulation of the RB-dependent pathway. It binds to both hypo- and hyperphosphorylated RB. The binding between RB and S18-2 is promoted when cytoplasmic S18-2 is targeted to the nucleus, and this disrupts the association of E2F1 with RB, as indicated by the increased level of free E2F1 in the nucleus. This presumably enhances the RB-dependent block to S-phase entry in the cell cycle. We have also found overexpression of the human S18-2 in immortalized primary rat embryonic fibroblasts (REFs) that showed properties of embryonic stem cells. Elevated expression of S18-2 in stem cells (our findings and analysis of published microarray data) raises the question of whether this protein co-operates with the RB protein in differentiation and carcinogenesis Additional Access Points: Mushtag, M. Kovalevska, L. Klein, G. Kashuba, E.