Type of document : Magazine article Edition cipher : Author(s) : Liubich L. D., Kovalevska L. M., Lisyany M. I., Semenova V. M., Malysheva T. A., Stayno L. P., Vaslovych V. V. Title : TGF-β1 expression by glioma C6 cells in vitro Place of publication : Экспериментальная онкология. - 2017. - Т. 39, № 4. - С. 258-263 (Cipher ЕУ12/2017/39/4) Notes : Bibliogr. at the end of the art. Annotation: The aim of the work was to study the impact of fetal rat brain cell supernatant (FRBCS) on the expression of transforming growth factor β1 (TGF-β1) and p53 in C6 cells of rat glioma in vitro. Materials and Methods: FRBCS was obtained from suspensions of fetal rat brain cells on the 14th (E14) day of gestation. C6 glioma cells were cultured for 48 h in the presence of FRBCS or FRBCS + anti-TGF-β1 monoclonal antibody. Immunocytochemical staining for TGF-β1 and p53 was performed. Results: The proportion of TGF-β1-immunopositive tumor cells in C6 glioma cultures was statistically significantly higher than in the control cell cultures of normal fetal rat brain. FRBCS reduced the proportion of TGF-β1-immunopositive tumor cells and increased the proportion of p53-immunopositive cells in C6 glioma cultures. In cells cultured with FRBCS + anti-TGF-β1 monoclonal antibody, the above effects of FRBCS were abrogated. Conclusion: The obtained results suggest that TGF-β1 seems to be responsible for decrease in TGF-β1 expression and increase in p53 expression in C6 glioma cells treated with FRBCS Additional Access Points: Liubich, L. D. Kovalevska, L. M. Lisyany, M. I. Semenova, V. M. Malysheva, T. A. Stayno, L. P. Vaslovych, V. V.