Ca[[p]]2+[[/p]]-dependent regulation of the Ca[[p]]2+[[/p]] concentration in the myometrium mitochondria. I. trifluoperazine effects on mitochondria membranes polarization and [Ca[[p]]2+[[/p]]][[d]]m[[/d]] [Text] = Ca[[p]]2+[[/p]]-залежна регуляція концентрації Ca[[p]]2+[[/p]] в мітохондріях міометрія. I. Ефекти трифлуоперазину на поляризацію мітохондріальних мембран та [Ca[[p]]2+[[/p]]][[d]]m[[/d]] / L. G. Babich [et al.] // Український біохімічний журнал. - 2016. - Т. 88, № 4. - P5-11. - Bibliogr. at the end of the art. Рубрики: Трифлуоперазин--Исследовательские технологии MeSH-головна: МИОМЕТРИЙ -- MYOMETRIUM (ультраструктура, химия) МИТОХОНДРИИ МЫШЦ -- MITOCHONDRIA, MUSCLE (ультраструктура, химия) КАЛЬЦИЕВЫЕ КАНАЛЫ -- CALCIUM CHANNELS (анализ) БИОМЕДИЦИНСКИЕ ИССЛЕДОВАНИЯ -- BIOMEDICAL RESEARCH (тенденции) Анотація: Ca[[p]]2+[[/p]]-dependent regulation of Ca[[p]]2+[[/p]] exchange in mitochondria is carried out with participation of calmodulin. We have shown previously that calmodulin antagonists reduced the level of mitochondrial membrane polarization and induced increase of the ionized Са concentration in both the mitochondrial matrix and cell cytoplasm. The concentration-dependent influence of trifluoperazine on the level ofpolarization of mitochondrial membranes has been shown in this work. The coordinates of the Hill graphs were used to calculate the constant K[[d]]05[[/d]] and the Hill coefficient. K[[d]]05[[/d]] was 24.4 ± 5 pM (n = 10). The Hill coefficient was 2.0 ± 0.2, indicating the presence of two centers of the trifluoperazine binding. We have also studied [Ca[[p]]2+[[/p]]][[d]]m[[/d]] changes, when incubating mitochondria in mediums of different composition: without ATP and ions of Mg (0-medium), in the presence of 3 mMMg (Mg-medium) and 3 mMMg + 3 mM ATP (Mg,ATP-medium). It was shown that the composition of the incubation medium affected the [Ca[[p]]2+[[/p]]][[d]]m[[/d]] values in the absence of exogenous Ca[[p]]2+[[/p]] and did not affect them in the presence of the latter. Preincubation of mitochondria in mediums of different composition with 25 pM trifluoperazine did not affect the [Ca[[p]]2+[[/p]]][[d]]m[[/d]] values both before and after the addition of 100 μΜ Са[[p]]2+[[/p]] to the incubation medium. It was concluded, that trifluoperazine depolarized myometrial mitochondria membranes in concentration-dependent manner. However, mitochondria preincubation with 25 pM trifluoperazine accompanied by 50% decrease in membrane polarization did not affect the [Ca[[p]]2+[[/p]]][[d]]m[[/d]] values Дод.точки доступу: Babich, L. G. Shlykov, S. G. Kushnarova, A. M. Kosterin, S. O. Вільних прим. немає