Monooxygenase system in Guerin’s carcinoma of rats under conditions of ω-3 polyunsaturated fatty acids administration [Text] = Стан монооксигеназної системи карциноми Герена щурів за введення ω-3 поліненасичених жирних кислот / M. M. Marchenko [et al.] // Український біохімічний журнал. - 2016. - Т. 88, № 4. - P48-56. - Bibliogr. at the end of the art. MeSH-головна: НОВООБРАЗОВАНИЯ ЭКСПЕРИМЕНТАЛЬНЫЕ -- NEOPLASMS, EXPERIMENTAL (метаболизм, терапия, этиология) БИОМЕДИЦИНСКИЕ ИССЛЕДОВАНИЯ -- BIOMEDICAL RESEARCH (методы, тенденции) БОЛЕЗНЬ, МОДЕЛИ НА ЖИВОТНЫХ -- DISEASE MODELS, ANIMAL ОКСИГЕНАЗЫ -- OXYGENASES (анализ, метаболизм) ЖИРНЫЕ КИСЛОТЫ, ОМЕГА-3 -- FATTY ACIDS, OMEGA-3 (анализ, терапевтическое применение) ЦИТОХРОМЫ B -- CYTOCHROMES B (анализ) ХОЛЕСТЕРИНА БОКОВУЮ ЦЕПЬ-РАСЩЕПЛЯЮЩИЙ ФЕРМЕНТ -- CHOLESTEROL SIDE-CHAIN CLEAVAGE ENZYME (анализ) Анотація: The aim of the study was to determine the variations of function in components of monooxygenase system (MOS) of rat Guerin’s carcinoma under ω-3 polyunsaturated fatty acids (PUFAs) administration. The activity of Guerin’s carcinoma microsomal NADH-cytochrome b[[d]]5[[/d]] reductase, the content and the rate of cytochrome b[[d]]5[[/d]] oxidation-reduction, the content and the rate of cytochrome Р450 oxidation-reduction have been investigated in rats with tumor under conditions of ω-3 PUFAs administration. ω-3 PUFAs supplementation before and after transplantation of Guerin’s carcinoma resulted in the increase of NADH-cytochrome b5 reductase activity and decrease of cytochrome b[[d]]5[[/d]] level in the Guerin’s carcinoma microsomal fraction in the logarithmic phases of carcinogenesis as compared to the tumor-bearing rats. Increased activity of NADH-cytochrome b[[d]]5[[/d]] reductase facilitates higher electron flow in redox-chain of MOS. Under decreased cytochrome b[[d]]5[[/d]] levels the electrons are transferred to oxygen, which leads to heightened generation of superoxide (O[[d]]2[[/d]][[p]].-[[/p]]) in comparison to control. It was shown, that the decrease of cytochrome P450 level in the Guerin’s carcinoma microsomal fraction in the logarithmic phases of oncogenesis under ω-3 PUFAs administration may be associated with its transition into an inactive form - cytochrome P420. This decrease in cytochrome P450 coincides with increased generation of superoxide by MOS oxygenase chain Дод.точки доступу: Marchenko, M. M. Ketsa, O. V. Shmarakov, I. O. Abutnaritsa, K. H. Вільних прим. немає