Parthenolide reduces gene transcription of prosurvival mediators in U937 cells / S. Mohammadi [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P30-35 : il. - 55 ref. MeSH-головна: ОСТЕОПОНТИН -- OSTEOPONTIN ТРАНСКРИПЦИИ ФАКТОР RELB -- TRANSCRIPTION FACTOR RELB БЕТА-КАТЕНИН -- BETA CATENIN Анотація: In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway or β-catenin have been considered. Aim: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN and β-catenin genes. Materials and Methods: The U937 cells were treated with PTL at concentrations of 4 μM (IC25) or 6 μM (IC50) and with OPN siRNA for MTT assay and colony forming assay. Western blot analysis using antibodies against OPN was performed with lysates of PTL-treated cells. Quantitative real-time polymerase chain reaction was performed using primers for OPN siRNA, RelB, AKT1, mTOR, PTEN and β-catenin. Results: PTL reduces OPN protein level and down-regulates RelB mRNA in U937 cell line. Suppression of OPN with siRNA increases the cytotoxic effects of PTL. Also, mRNA expression of AKT1, mTOR, PTEN, and β-catenin decreases with PTL or OPN siRNA. Conclusion: Sensitivity of U937 cells to PTL can be associated with the reduction in expression of prosurvival mediators Дод.точки доступу: Mohammadi, S. Zahedpanah, M. Nikbakht, M. Shaiegan, M. Hamidollah Ghaffari, S. Nikugoftar, M. Rahmani, B. Asl, D. Вільних прим. немає