Kolupaev, Yu. E. Induction of plant cells heat resistance by hydrogen sulfide donor is mediated by H2O2 generation with participation of NADPH oxidase and superoxide dismutase / Yu. E. Kolupaev, E. N. Firsova, T. O. Yastreb // The Ukrainian Biochemical Journal. - 2017. - Том 89, N 4. - P34-42 MeSH-головна: ПШЕНИЦА -- TRITICUM (действие лекарственных препаратов) НАТРИЯ СОЕДИНЕНИЯ -- SODIUM COMPOUNDS Анотація: The participation of enzymatic systems carrying out generation and conversion of reactive oxygen species (ROS), in realization of the stress-protective effect of hydrogen sulfide (H[[d]]2[[/d]]S) on wheat coleoptile cells was investigated. It has been shown that the treatment of isolated coleoptiles with a 100 μM hydrogen sulfide donor sodium hydrosulfide (NaHS) caused a transient enhancement of the generation of superoxide anion radical (O[[d]]2[[/d]][[p]]•–[[/p]]), an increase of hydrogen peroxide content and superoxide dismutase (SOD) activity in them. The increase in ROS generation was eliminated by the inhibitor of NADPH oxidase imidazole, but not by the peroxidase inhibitor sodium azide. Treatment of coleoptiles with SOD inhibitor sodium diethyldithiocarbamate (DDC) enhanced the generation of O[[d]]2[[/d][[p]]][[p]]•–[[/p]] and neutralized the effect of increasing H[[d]]2[[/d]]O2 content induced by NaHS. One day after treatment with the H[[d]]2[[/d]]S donor, the generation of ROS decreased to a control level, while the activity of antioxidant enzymes increased markedly and the resistance of coleoptiles to damaging heating was increased. These effects of the hydrogen sulfide donor were eliminated by coleoptiles’ treatment with inhibitors of NADPH oxidase (imidazole) and SOD (DDC). It was concluded that both NADPH oxidase, generating O[[d]]2[[/d]][[p]]•–[[/p]] , and SOD, which turns it into H[[d]]2[[/d]]O2 performing signaling functions, are involved in the formation of a signal that induces protective systems and causes an increase in heat resistance of plant cells Дод.точки доступу: Firsova, E. N. Yastreb, T. O. Вільних прим. немає |