Reversible pH-dependent activation/inactivation of CF1-ATPase of spinach chloroplasts / A. P. Khomochkin [et al.] // The Ukrainian Biochemical Journal. - 2017. - Том 89, N 4. - P43-48 MeSH-головна: ХЛОРОПЛАСТЫ -- CHLOROPLASTS (действие лекарственных препаратов) ШПИНАТ ОГОРОДНЫЙ -- SPINACIA OLERACEA (действие лекарственных препаратов) ДИАКАРБ -- ACETAZOLAMIDE (метаболизм) Анотація: The aim of the work was to study the reverse pH-dependent regulation of the enzymatic activity of the catalytic part of ATP synthase (EC 3.6.3.14) of chloroplast – coupling factor CF1. It was shown that the short-term incubation of isolated CF1 in the media with pH 4.5 or 3.5 leads to inactivation of Ca[[p]]2+[[/p]]-ATPase, which is rapidly (t1/2 ~ 1 min) restored in the medium containing 0.5-10 mM bicarbonate at pH 7.8. After acid treatment, the rate of Mg[[p]]2+[[/p]]-ATPase reaction was also stimulated in the presence of 1 mM bicarbonate (рН 7.8; 37 °С). The increase in Ca[[p]]2+[[/p]]– and Mg[[p]]2+[[/p]]-АТР activity of CF[[d]]1[[/d]] associated with the addition of NaHCO[[d]]3[[/d]] solution was completely eliminated after the introduction of 50 mM acetazolamide – a specific inhibitor of carbonic anhydrase. The obtained results suggest the existence of the bound bicarbonate in the CF[[d]]1[[/d]] structure, which apparently participates in proton transfer. Дод.точки доступу: Khomochkin, A. P. Onoiko, O. B. Semenikhin, A. V. Zolotareva, O. K. Вільних прим. немає