Ca2+-dependent regulation of fibrinolytic system activation on fibrin(ogen) D-domains / T. A. Yatsenko [et al.] // The Ukrainian Biochemical Journal. - 2017. - Том 89, N 5. - P21-31 MeSH-головна: ФИБРИН -- FIBRIN (метаболизм) Анотація: In the present study, we investigated whether calcium content modulation in D-domains of fibrin(ogen) was involved in fibrinolytic process activation. To investigate the effect of Ca[[p]]2+[[/p]]-dependent changes in D-domains two types of fibrinogen fragments D and cross-linked fibrin fragments DD were obtained from plasmin hydrolysate of human fibrin(ogen): chelator-treated and without chelating agents. The study of plasminogen activation by tissue-type plasminogen activator on D- and DD-fragments had shown the intensification of plasmin formation in case of EDTA pretreatment of fragments. The proenzyme activation rate on DD also increased in the presence of EGTA in concentration-dependent manner. Potentiating effect of EGTA-pretreated DD-fragment on plasminogen activation by tPA was decreased in the presence of Ca[[p]]2+[[/p]]. Activation rate reduction was observed according to the increase of CaCl[[d]]2[[/d]] concentration in the reaction medium. The intensification of plasminogen activation potentiation by chelator-treated fibrin(ogen) D-domain containing fragments and subsequent potentiation decrease in the presence of Ca[[p]]2+[[/p]] indicated the requirement of Ca[[p]]2+[[/p]]-dependent changes in D-domains for plasminogen activation sites exposure and initiation of fibrinolysis Дод.точки доступу: Yatsenko, T. A. Rybachuk, V. М. Kharchenko, S. M. Grinenko, T. V. Вільних прим. немає