Babina, U. M. Comparative study of infiltration between three local anesthetics: bupivacaine, lidocaine and ropivakain on wound healing in rats [Text] / U. M. Babina, D. V. Dmytriiev, O. A. Nazarchur // Біль, знеболювання і інтенс. терапія. - 2019. - N 3. - Библиогр. в конце ст. Анотація: The aim of this study was to comparative investigation the effects of saline solution, bupivacaine, lidocaine and ropivacaine infiltration on wound healing in rats. MATERIAL AND METHODS Fifty onemale Wistar rats weighing between 250-300 grams were randomly separated into four groups. Surgical procedures were done under general anesthesia, induced by intraperitoneal injection of 75 mg.kg-1 ketamine. The hair on the back of the animal was shaved after the loss of cornea reflex and extremity drawing response were diminished. The area of the incision was cleaned with povidione iodine and was wiped dry with sterile gauges after two minutes. The areas of the incisions were subcutaneously infiltrated with 3 ml doses of the study drug. The rats in the groups were infiltrated with normal saline in control group (Group C, n = 13), 2% lidocaine in lidocaine group (Group L ,n = 13), 0.5% bupivacaine in bupivacaine group (Group B, n = 13), and 0,75% ropivacaine in ropivacaine group (Group R, n = 12). Breaking-strength measurements, collagen bundle counting, and histopathologic evaluation were evaluated in the tissue samples taken from the rats. RESULTS Comparing the control group with the groups where bupivacaine and lidocaine were used for wound infiltration, collagen production was lower, breaking-strength measurements showed reduced resistance while significantly high edema, vascularity, inflammation scores were found (p 0.0122). Between the control and the ropivacaine group there were no significant differences in collagen production, breaking-strength measurements, and edema, vascularity, inflammation scores (p 0.0118). CONCLUSION Surgical wound infiltration has been proven to be an effective analgesic and is widely used for postoperative pain relief after abdominal hysterectomy, cesarean section, inguinal hernia repair, lumbar disc hernia, prostatectomy and similar surgeries. Frequently used local anesthetic agents are lidocaine, bupivacaine, ropivacaine. Research on the effect of these local anesthetic agents used for wound infiltration on wound healing is limited and results are controversia. In our study, we found bupivacaine and lidocaine reduced the collagen production, wound breaking strength, and caused significantly high scores for edema, vascularity, and inflammation when compared to the control group. There was no significant difference between the control and the ropivacaine group. Results of this experimental preliminary study on rats support the idea that ropivacaine can be used for wound infiltration anesthesia without adverse effect on the surgical healing process. These results need to be verified in humans Дод.точки доступу: Dmytriiev, D. V. Nazarchuk, O. A. Вільних прим. немає |