Epiphytic Bacteria Bacillus subtilis UzNU-18 from Jerusalem Artichoke (Helianthus tuberosus L.) – the Active Biocontrol Agent of Phytopathogenic Microorganisms [Text] / К. Davranov [et al.] // Мікробіологічний журнал. - 2019. - Том 81, N 3. - P27-37 MeSH-головна: ПОЧВЫ МИКРОБИОЛОГИЯ -- SOIL MICROBIOLOGY РАСТЕНИЙ ЛИСТЬЯ -- PLANT LEAVES (микробиология) СЕННАЯ ПАЛОЧКА -- BACILLUS SUBTILIS (химия) ГРИБЫ -- FUNGI (патогенность) Кл.слова (ненормовані): ИЕРАСУЛИМСКИЙ АРТИШОК -- ТОПИНАМБУР Анотація: We isolated a new strain of bacteria belonging to the genus Bacillus from the green leaves of Jerusalem artichoke also known as topinambour (Helianthus tuberosus L.). The aim of this work was to study physiological and biochemical features of the epiphytic strain Bacillus subtilis UzNU-18, its antagonistic activity, and conditions of formation of antimicrobial metabolites synthesized by this strain. Methods. Standard microbiological, molecular biological, biochemical, chromatographical and statistical methods was used. Results. The isolate UzNU-18 was isolated from the surface of green leaves of Jerusalem artichoke also known as topinambour (Helianthus tuberosus L). Upon screening, this isolate was selected as the best growth inhibitor of the tested phytopathogenic fungi species (Fusarium oxysporum, Fusarium culmorum, Fusarium solani, Rhizoctonia solani, Phytophtora capsici, Alteranria alternata) and bacteria species (Pseudomonas syringae, Erwinia carotovora, Xanthomonas beticola). The 16S rRNA sequence analysis showed that the isolate (GenBank accession # MH312004) belongs to Bacillus subtilis. Chromatography mass spectrometry analysis showed that Bacillus subtilis UzNU-18 produces antagonistic substance - 2.4-dimethylpentanone-3. Conclusions. Inoculation of soil with Bacillus subtilis UzNU-18 culture liquid reduced the infectious content in soil and contributed to better growth of wheat seedlings Дод.точки доступу: Davranov, К. Shurigin, V. Mammadiev, A. Ruzimova, K. Вільних прим. немає