Hrushanyk, N. V.     Oxidative stress regulation in the yeast Ogataea polymorpha producer of human α-synuclein / N. V. Hrushanyk, O. V. Stasyk, O. G. Stasyk // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P120-133 MeSH-головна: ПАРКИНСОНА БОЛЕЗНЬ -- PARKINSON DISEASE (патофизиология) ДРОЖЖИ -- YEASTS (действие лекарственных препаратов) ГЛЮКОЗА -- GLUCOSE (метаболизм) ОКСИДАТИВНЫЙ СТРЕСС -- OXIDATIVE STRESS (физиология) Анотація: In this study we analyzed how exogenous glucose levels affect enzymatic and non-enzymatic antioxidant defense systems and markers of oxidative stress in cells of the methylotrophic yeast Ogataea polymorpha producing recombinant human α-synuclein, implicated in pathogenesis of neurodegenerative Parkinson’s disease (PD). We found that glucose depletion up-induced activity of antioxidant enzymes superoxide dismutase, and catalase, and increased content of reduced and oxidized glutathione in the cells cultivated in the medium with 0.1% glucose, as compared to physiological growth condition (1% glucose-containing medium). In addition, low glucose concentration in the medium upregulated content of proteins carbonyl groups and of products of lipid peroxidation. Notably, the shift in the equilibrium toward pro-oxidant changes was similar for recombinant α-synuclein producer and parental wild-type strain. Thus, glucose limitation leads to the overproduction of reactive oxygen species in the methylotrophic yeast cells independently of the recombinant human α-synuclein production Дод.точки доступу: Stasyk, O. V. Stasyk, O. G. Вільних прим. немає