Форма документа : Стаття із журналу Шифр видання : Автор(и) : Nozdrenko D. M., Miroshnychenko M. S., Soroсa V. M., Korchinska L. V., Zavodovskiy D. O. Назва : The effect of chlorpyrifos upon ATPase activity of sarcoplasmic reticulum and biomechanics of skeletal muscle contraction Паралельн. назви :Вплив хлорпірифосу на активність АТРази саркоплазматичного ретикулума та біомеханіку скорочення скелетних м’язів Місце публікування : Український біохімічний журнал. - 2016. - Т. 88, № 2. - С. 82-88 (Шифр УУ1/2016/88/2) Примітки : Bibliogr. at the end of the art. MeSH-головна: ХЛОРПИРИФОС -- CHLORPYRIFOS КАЛЬЦИЙ-ТРАНСПОРТИРУЮЩИЕ АТФАЗЫ САРКОПЛАЗМАТИЧЕСКОГО РЕТИКУЛУМА -- SARCOPLASMIC RETICULUM CALCIUM-TRANSPORTING ATPASES АТФАЗА-CA(2+) MG(2+) -- CA(2+) MG(2+)-ATPASE МЫШЕЧНОЕ СОКРАЩЕНИЕ -- MUSCLE CONTRACTION ЦИТОЛОГИЧЕСКИЕ МЕТОДЫ -- CYTOLOGICAL TECHNIQUES Анотація: We investigated the effect of chlorpyrifos, an organophosphate insecticide, on Ca2+,Mg2+-ATPase activity of sarcoplasmic reticulum and on contraction dynamics (force and length changes) of Rana temporaria m. tibialis anterior muscle fiber bundles. All of the used concentrations of chlorpyrifos (10-6 to 10-5 M) caused decrease of Ca2+,Mg2+-ATPase activity. The inhibition of Ca2+,Mg2+-ATPase activity by chlorpyriphos in concentrations of 10-6 M to 7.5·10-6 M is due to permeation of sarcoplasmic reticulum rather than due to direct enzyme inhibition by organophosphate insecticides. The inhibitory properties of the compound were higher at increased concentration and exposure timeframes. Chlorpyrifos in concentration range of 10-6 to 7.5·10-6 M causes changes in muscle fiber response force that were more pronounced than changes in contractile length. We demonstrated inhibition of Ca2+,Mg2+-ATPase activity caused by noncholinergic effects of chlorpyriphos. It is possible to conclude that influence of organophosphate insecticides happens not only in the neuromuscular transmission but also on the level of subcellular structures Дод.точки доступу: Nozdrenko, D. M. Miroshnychenko, M. S. Soroсa, V. M. Korchinska, L. V. Zavodovskiy, D. O.