Форма документа : Стаття із журналу Шифр видання : Автор(и) : Nikolaienko T. V., Nikulina V. V., Shelest D. V., Garmanchuk L. V. Назва : The mechanism of VEGF-mediated endothelial cells survival and proliferation in conditions of unfed-culture Паралельн. назви :Механізми VEGF-опосередкованого виживання та проліферації ендотеліальних клітин в умовах довготривалого культивування без заміни середовища Місце публікування : Український біохімічний журнал. - 2016. - Т. 88, № 4. - С. 12-19 (Шифр УУ1/2016/88/4) Примітки : Bibliogr. at the end of the art. MeSH-головна: НЕОВАСКУЛЯРИЗАЦИЯ ПАТОЛОГИЧЕСКАЯ -- NEOVASCULARIZATION, PATHOLOGIC ЭНДОТЕЛИАЛЬНЫЕ КЛЕТКИ -- ENDOTHELIAL CELLS БОЛЕЗНЬ, МОДЕЛИ НА ЖИВОТНЫХ -- DISEASE MODELS, ANIMAL КАРЦИНОМА ЛЬЮИС ЛЕГКОГО -- CARCINOMA, LEWIS LUNG БЕЛКИ НОВООБРАЗОВАНИЙ -- NEOPLASM PROTEINS КЛЕТКИ ПРОЛИФЕРАЦИЯ -- CELL PROLIFERATION КРОВЕНОСНЫХ СОСУДОВ ЭНДОТЕЛИАЛЬНЫЕ ФАКТОРЫ РОСТА -- VASCULAR ENDOTHELIAL GROWTH FACTORS Анотація: The mechanisms of VEGF-mediated effects on endothelial cells during cancer development and progression is not clear. In present study the biological effects of VEGF, VEGF-rich culture medium of peritoneal macrophages from mice with Lewis lung carcinoma were studied on MAEC cell line under conditions of unfed culture. We have shown that VEGF increased cell proliferation by the 5[[p]]th[[/p]] day of culturing vs control and anti- VEGF-treated cells. This effect was associated with increased consumption of glucose and NO production by the 2[[p]]nd[[/p]] day while decreased - on the 5[[p]]th[[/p]] day of cell culturing. VEGF-mediated NO production was dependent on Ca[[p]]2+[[/p]] ions. Block of Ca[[p]]2+[[/p]]-channels (LaCI[[d]]3[[/d]]) had more pronounced inhibitory effect vs chelator of Ca[[p]]2+[[/p]] ions (EDTA). It was shown that peritoneal macrophages are the main suppliers of VEGF at tumor angiogenesis, as evidenced by the data obtained on model system of endothelial cells synchronized in G[[d]]0[[/d]]/G[[d]]1[[/d]] phase Дод.точки доступу: Nikolaienko, T. V. Nikulina, V. V. Shelest, D. V. Garmanchuk, L. V.