Форма документа : Стаття із журналу Шифр видання : Автор(и) : Holota Yu. V., Olefir Ya. A., Dovbynchuk T. V., Tolstanova G. M. Назва : Carbohydrate composition of rat intestine surface mucus layer after ceftriaxone treatment Паралельн. назви :Вуглеводний склад поверхневого слизу кишечника щурів після введення цефтриаксону Місце публікування : Український біохімічний журнал. - 2016. - Т. 88, № 6. - С. 35-44 (Шифр УУ1/2016/88/6) Примітки : Bibliogr. at the end of the art. MeSH-головна: МОДЕЛИ НА ЖИВОТНЫХ -- MODELS, ANIMAL КИШЕЧНИКА СЛИЗИСТАЯ ОБОЛОЧКА -- INTESTINAL MUCOSA ЦЕФТРИАКСОН -- CEFTRIAXONE ГЛИКОПРОТЕИНЫ -- GLYCOPROTEINS КРЫСЫ ИНБРЕДНОЙ ЛИНИИ BB -- RATS, INBRED BB УГЛЕВОДНЫЙ ОБМЕН -- CARBOHYDRATE METABOLISM Анотація: The epidemiological studies have shown that antibiotic treatment increases the susceptibility to inflammatory bowel disease development. The disturbance of mucus layer integrity might be one of the possible mechanisms. The aim of the present study was to investigate the effect of antibiotic ceftriaxone treatment on glycoproteins level and its carbohydrate composition in surface mucus layer of rat intestine. The study was done on male Wistar rats (140-160 g). Ceftriaxone (300 mg/kg, i.m.) was administered once a day for 14 days. The surface mucus from terminal ileum and colon were collected on the 15th, 29th and 72nd days of the experiment. Total level of mucus glycoproteins, hexoses, hexosamines, fucose and sialic acids were measured. Ceftriaxone administration did not affect the levels of glycoproteins in rat ileum. In the colon, the levels of glycoprotein were 1.3-fold decreased (Р 0.05) on the 72nd day of the experiment. These changes were accompanied by the 1.2-fold decrease of hexoses (Р 0.05) and 3.1-fold (Р 0.05) decrease of fucose level and 1.5-fold (Р 0.05) increase of the levels of sialic acids in the surface mucus of the rat colon. Thus, ceftriaxone administration induces the long-term changes in the levels of glycoproteins and carbohydrates composition in the rat colon surface mucus. This could potentially explain the susceptibility to inflammatory bowel diseases development Дод.точки доступу: Holota, Yu. V. Olefir, Ya. A. Dovbynchuk, T. V. Tolstanova, G. M.