Форма документа : Стаття із журналу Шифр видання : Автор(и) : Afanasieva K. S., Chopei M. I., Lozovik A. V., Rushkovsky S. R., Sivolob A. V. Назва : Redistribution of DNA loop domains in human lymphocytes under blast transformation with interleukin 2 Паралельн. назви :Перерозподіл петельних доменів ДНК у лімфоцитах людини у разі бласттрансформації за впливу інтерлейкіну 2 Місце публікування : Український біохімічний журнал. - 2016. - Т. 88, № 6. - С. 45-51 (Шифр УУ1/2016/88/6) Примітки : Bibliogr. at the end of the art. MeSH-головна: ТРАНСКРИПЦИЮ АКТИВИРУЮЩИЕ ФАКТОРЫ -- ACTIVATING TRANSCRIPTION FACTORS ЛИМФОЦИТЫ -- LYMPHOCYTES ИНТЕРЛЕЙКИН-2 -- INTERLEUKIN-2 ДНК-КОМЕТ МЕТОД -- COMET ASSAY КЛЕТКИ ЯДРО -- CELL NUCLEUS Анотація: At higher order levels chromatin fibers in interphase nuclei are organized into loop domains. Gene regulatory elements (promoters and enhancers) are often located near the sites of loop attachments. Therefore, loop domains play a key role in regulation of cell transcriptional activity. We investigated the kinetics of DNA loop exit during single cell gel electrophoresis (the comet assay) of nucleoids obtained from two cell types that differ in their synthetic activity – human lymphocytes and lymphoblasts. Lymphocyte activation and transformation into lymphoblasts (blast transformation) was performed with interleukin 2. The results obtained suggest that a rearrangement of the loops occurs after lymphocyte activation. After blast transformation we observed an increase of the amount of loop domains on the surface of nucleoids against a decrease of the inner loop fraction. Therefore, the comet assay can be used for detection of large-scale changes in the cell nucleus that follow changes in cell functional state Дод.точки доступу: Afanasieva, K. S. Chopei, M. I. Lozovik, A. V. Rushkovsky, S. R. Sivolob, A. V.