Форма документа : Стаття із журналу Шифр видання : Автор(и) : Volkova N. O., Yukhta M.S., Goltsev A. M. Назва : Potentiation of cryopreserved rat adipose-derived multipotent mesenchymal stromal cells by BMP-12 in vitro for the treatment of tendinopathy Місце публікування : Клітинна та органна трансплантологія. - 2018. - Том 6, N 2. - С. 131-136 (Шифр КУ53/2018/6/2) Примітки : Bibliogr. at the end of the art. Анотація: Bone morphogenetic proteins (BMP), which are part of TGF-b superfamily, have a stimulating effect on bone formation, as well as the proliferation oftenocytes and fibroblasts, which form the tendon tissueThe PURPOSE of the study was to determine the effect of BMP-12 on the morphofunctional characteristics of cryopreserved rat adipose tissue- derived multipotent mesenchymal stromal cells (AT-MMSCs) and to investigate the effects of the use ofAT-MMSCs pre-cultured with BMP-12 on the tendinopathy modelMATERIALS AND METHODS. MMSCs were obtained from the adipose tissue of rats. Cryopreservation was carried out under the protection of 10 % DMSO with the addition of 20 % fetal bovine serum at a cooling rate of 1 °C/min to -80 °С and subsequent transfer to liquid nitrogen. When culturing AT-MMSCs, the BMP-12 was added at the concentration of 50 ng/mL. The ability of cells to proliferation (by MTT-test) and the synthesis of collagen I and III types were evaluated. Rats with modeled degenerative-dystrophic injury to the Achilles tendons were locally administered with 0.25-1 № AT-MMSCs orAT-MMSCs+BMP-12 in the defect zone. The animals injected with saline provided control group. On the 21st day after the treatment, a histological, immunofluorescence and biomechanical studies were performedRESULTS. The application of BMP-12 results in the decrease in proliferation ofAT-MMSCs along with an increase in the relative number of cells that synthesize collagen I and III types relative to AT-MMSCs cultivated under standard conditions. The injection ofAT-MMSCs promotes the activation of regenerative processes in damaged tendons. The use of AT-MMSCs+BMP-12 accelerates the histological structure, strength, and the content of collagen I and III types in the Achilles tendons of animals with degenerative-dystrophic damage compared to the cells without BMP-12CONCLUSIONS BMP-12 can be used as a stimulating agent for the tenogenic differentiation ofAT-MMSCs before transplantation into the damaged tendon tissue Дод.точки доступу: Yukhta, M.S. Goltsev, A. M.