Форма документа : Стаття із журналу Шифр видання : Автор(и) : Veklich T. O., Labyntseva R. D., Shkrabak O. A., Tsymbalyuk O. V., Rodik R. V., Kalchenko V. I., Kosterin S. O. Назва : Inhibition of Na(+),K(+)-ATPase and activation of myosin ATPase by CALIX[4]A RENE C-107 cause stimulation of isolated smooth muscle contractile activity Місце публікування : The Ukrainian biochemical journal. - Київ, 2020. - Vol. 92, № 1. - С. 21-30 (Шифр УУ60/2020/92/1) MeSH-головна: МЫШЦЫ -- MUSCLES Анотація: The discovery of compounds that might modify myometrial contractility is an important area of researches. In our previous experiments, we found that some representatives of macrocyclic compounds fami­ly – calix[4]arenes – can modify the enzymatic and transport activity of membrane-bound cation-transport ATP hydrolases. The aim of this work was to study and compare the effect of calix[4]arene C-107 on the enzymatic activities of Mg2+-dependent ATPases of the uterine smooth muscle, namely: ouabain-sensitive Na+,K+-ATPase, plasma membrane Ca2+-independent “basal” Mg2+-ATPase, ATPase of the actomyosin complex and myosin subfragment-1, with effect on the contractile activity of the myometrium. It was shown that calix[4]arene C-107 efficiently inhibited myometrium Na+,K+-ATPase (I50 = 54 ± 6 nM) selectively to other ATP-hydrolases of the plasma membrane and simultaneously activated the enzymatic activity of the myosin ATPase of smooth muscles (A50 = 9.6 ± 0.7 μM). Such reciprocal biochemical effects led to the stimulation of the smooth muscle contractile activity that was demonstrated by the tensometric method using different isolated smooth muscles. Calix[4]arene С-107 was shown to stimulate the increase of the tonic component of myometrium contractions induced by oxytocin, as well as contractions of the caecum muscles induced by high-potassium solution or acetylcholine, and to maintain increased tension for a long time. Thus, calix[4]arene C-107 is a prospective compound for enhancing the smooth muscle basal tone and/or contraction in case of hypotonic dysfunctions Дод.точки доступу: Veklich, T. O. Labyntseva, R. D. Shkrabak, O. A. Tsymbalyuk, O. V. Rodik, R. V. Kalchenko, V. I. Kosterin, S. O.