Форма документа : Стаття із журналу Шифр видання : Автор(и) : Gulevskyy A. K., Akhatova Yu. S., Nikolchenko A. Yu. Назва : Cryoprotective agents affect amino acids incorporation into total proteins in cells of lymphoid organs and liver of experimental animals Місце публікування : The Ukrainian biochemical journal. - Київ, 2020. - Vol. 92, № 5. - С. 41-49 (Шифр УУ60/2020/92/5) MeSH-головна: ПЕЧЕНЬ -- LIVER ЛИМФОИДНАЯ ТКАНЬ -- LYMPHOID TISSUE КРИОПРОТЕКТОРЫ -- CRYOPROTECTIVE AGENTS АМИНОКИСЛОТЫ -- AMINO ACIDS ЭУКАРИОТЫ -- EUKARYOTA Анотація: The effect of penetrating (glycerol, DMSO) and poorly penetrating (PEG-400) cryoprotective agents on labeled amino acids incorporation into de novo synthesized proteins in the cells of mice thymus, lymph nodes, spleen and cell-free rat liver extract was studied. Cryoprotective agents within the range of concentrations which provide a cryoprotective effect were found to inhibit significantly protein synthesis in cell-free systems under investigation. The most effective inhibition was exerted by polymeric cryoprotective agent PEG-400. Cryoprotective agents more efficiently inhibited protein synthesis at a cell level as compared with that in cell-free system that was likely associated with their effect on amino acids transport system. An inhibitory effect of cryoprotective agents on the protein synthesizing apparatus of cells was determined to be Mg2+-dependent and reversible Дод.точки доступу: Akhatova, Yu. S. Nikolchenko, A. Yu. |