Форма документа : Стаття із журналу Шифр видання : Автор(и) : Hrushanyk N. V., Stasyk O. V., Stasyk O. G. Назва : Oxidative stress regulation in the yeast Ogataea polymorpha producer of human α-synuclein Місце публікування : The Ukrainian biochemical journal. - Київ, 2020. - Vol. 92, № 5. - С. 120-133 (Шифр УУ60/2020/92/5) MeSH-головна: ПАРКИНСОНА БОЛЕЗНЬ -- PARKINSON DISEASE ДРОЖЖИ -- YEASTS ГЛЮКОЗА -- GLUCOSE ОКСИДАТИВНЫЙ СТРЕСС -- OXIDATIVE STRESS Анотація: In this study we analyzed how exogenous glucose levels affect enzymatic and non-enzymatic antioxidant defense systems and markers of oxidative stress in cells of the methylotrophic yeast Ogataea polymorpha producing recombinant human α-synuclein, implicated in pathogenesis of neurodegenerative Parkinson’s disease (PD). We found that glucose depletion up-induced activity of antioxidant enzymes superoxide dismutase, and catalase, and increased content of reduced and oxidized glutathione in the cells cultivated in the medium with 0.1% glucose, as compared to physiological growth condition (1% glucose-containing medium). In addition, low glucose concentration in the medium upregulated content of proteins carbonyl groups and of products of lipid peroxidation. Notably, the shift in the equilibrium toward pro-oxidant changes was similar for recombinant α-synuclein producer and parental wild-type strain. Thus, glucose limitation leads to the overproduction of reactive oxygen species in the methylotrophic yeast cells independently of the recombinant human α-synuclein production Дод.точки доступу: Stasyk, O. V. Stasyk, O. G.