Форма документа : Стаття із журналу Шифр видання : Автор(и) : Kuz I. O., Akimov O. Ye., Kostenko V. O., Sheshukova O. V., Maksymenko A. I., Pysarenko O. A. Назва : Functioning of NO-cycle in the saliva of children with type 1 diabetes mellitus Місце публікування : Пробл. ендокринної патології. - Харьков, 2021. - N 4. - С. 34-39 (Шифр ПУ17/2021/4) MeSH-головна: ДИАБЕТ САХАРНЫЙ, ТИП 1 -- DIABETES MELLITUS, TYPE 1 ДЕТИ -- CHILD АЗОТА ОКИСИ СИНТАЗА -- NITRIC OXIDE SYNTHASE СЛЮНА -- SALIVA Анотація: The presence of type 1 diabetes mellitus in children is a major risk factor for periodontal disease. The aim of research work was to determine the activity of NO-synthase and arginase in saliva in children age with insulin-dependent diabetes mellitus. Materials and methods We examined 82 children including 56 children with type 1 diabetes mellitus and 26 children without somatic diseases. NO-synthase (NOS) activity was determined by the difference in nitrite concentration before and after incubation of tissue homogenate. Determining arginase activity was based on analysis the difference in the concentration of L-ornithine before and after incubation in phosphate buffer solution. Statistical processing was performed using Microsoft Office Excel. Research results and their discussion The violation of the indicators’ balance between groups 1 and 3 showed us a decrease in regenerative capacity in the mucous membrane in persons with type 1 diabetes mellitus. Increased ARG activity in group 4 children may lead to rivarly between NOS and ARG for L-arginine. Increased ARG activity in groups 2 and 4 compared with group 1 indicated an adaptive response aimed at repairing gum damage. Based on this, increased NO production from NOS is a consequence of insulin deficiency (systemic factor). Conclusions. The combination of systemic factor (type 1 diabetes mellitus) and local (chronic catarrhal gingivitis) leads to dysregulation of the NO-cycle and increasing of competition between NOS and ARG Дод.точки доступу: Kuz, I. O. Akimov, O. Ye. Kostenko, V. O. Sheshukova, O. V. Maksymenko, A. I. Pysarenko, O. A.