Вид документа : Статья из журнала Шифр издания : Автор(ы) : Sokolik V. V., Koliada O. K., Shulga S. M. Заглавие : Effect of β-amyloid peptide 42 on the dynamics of expression and formation of Аβ(40), IL-1β, TNFα, IL-6, IL-10 by peripheral blood mononuclear cells in vitro and its correction by curcumin Параллельн. заглавия :Вплив β-амілоїдного пептиду 42 на динаміку експресії і утворення Аβ(40), IL-1β, TNFα, IL-6, IL-10 мононуклеарами периферійної крові in vitro та його корекція куркуміном Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 1. - С. 109-118 (Шифр УУ1/2016/88/1) Примечания : Бібліогр.: в кінці ст. MeSH-главная: АМИЛОИДА БЕТА-ПЕПТИДЫ -- AMYLOID BETA-PEPTIDES КУРКУМИН -- CURCUMIN МОНОНУКЛЕАРНАЯ ФАГОЦИТАРНАЯ СИСТЕМА -- MONONUCLEAR PHAGOCYTE SYSTEM ЦИТОКИН-ИНДУЦИРОВАННЫЕ КЛЕТКИ-КИЛЛЕРЫ -- CYTOKINE-INDUCED KILLER CELLS ЗДОРОВЫЕ ДОБРОВОЛЬЦЫ -- HEALTHY VOLUNTEERS Аннотация: The toxic effect of Αβ-oligomers accompanies chronic inflammation, with cytokines as main mediators. Therefore, the cytokine link of inflammation becomes a new target on the way to restrain amyloidosis. The aim of the study was the effect ofaggregated Аβ42 on the dynamics of expression andformation of endogenous Αβ and cytokines (IL-φ, TNFα, IL-6, IL-10) by peripheral blood mononuclear cells in vitro and its correction by curcumin. A suspension of mononuclear cells isolated ex tempore using ficoll-urografin gradient from venous blood samples of healthy volunteers were used to study the effects of Αβ (15 nM), curcumin (54pM), and their combined action (at similar concentrations) in time dynamics: 0, 1, 3, 6 and 24 h incubation at 37 °C. Polymerase chain reaction with appropriate primers was used to determine the relative expression of mRNA for ΑβΡΡ, TNFa, IL-Ιβ, IL-6, IL-10 and enzyme-linked immunosorbent assay - to determine the content of Αβ40 and cytokines in mononuclear suspension during all periods of incubation. The individual dynamics ΑβΡΡ and cytokine expression was shown under the action of the Αβ2 which had influence on the content of Αβ0 TNFa, IL-1ff IL-6 and IL-10 in mononuclear suspension. Curcumin displayed the inhibitory effect on gene expression of ΑβΡΡ, TNFa and IL6, which resulted in the decrease of the level of these two cytokines and Αβ40. Thus, the dynamics of anti-inflammatory effect of curcumin in vitro for transcriptional and translational levels of cytokine’s formation by mononuclear cells was shown in the work. Direct inhibitory effect of curcumin on the concentration of endogenous Αβ40 during the 24 h incubation in conditions of toxic action of Αβ aggregates was established Доп.точки доступа: Koliada, O. K. Shulga, S. M.