Вид документа : Статья из журнала Шифр издания : Автор(ы) : Sydor R. I., Khranovska N. M., Skachkova O. V., Skivka L. M. Заглавие : The effect of perioperative analgesic drugs omnopon and dexketoprofen on the functional activity of immune cells in murine model of tumor surgery Параллельн. заглавия :Вплив знеболювання омнопоном та декскетопрофеном на функціональний стан імунних клітин мишей на моделі хірургічного видалення пухлини Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 4. - С. 40-47 (Шифр УУ1/2016/88/4) Примечания : Bibliogr. at the end of the art. Предметные рубрики: Декскетопрофен-- тер прим Омнопон-- Исследования, характеристики MeSH-главная: МОДЕЛИ НА ЖИВОТНЫХ -- MODELS, ANIMAL БИОМЕДИЦИНСКИЕ ИССЛЕДОВАНИЯ -- BIOMEDICAL RESEARCH НОВООБРАЗОВАНИЯ ЭКСПЕРИМЕНТАЛЬНЫЕ -- NEOPLASMS, EXPERIMENTAL АНЕСТЕЗИРУЮЩИЕ СРЕДСТВА -- ANESTHETICS Аннотация: We aimed to investigate the effect of perioperative analgesia with nonselective cyclooxygenase-2 inhibitor dexketoprofen and opioid drug omnopon on the functional activity of immune cells in tumor excision murine model. Lewis lung carcinoma cells were transplanted into hind paw of C57/black mice. On the 23[[p]]th[[/p]] day tumor was removed. Analgesic drugs were injected 30 min before and once a day for 3 days after the surgery. Biological material was obtained a day before, 1 day and 3 days after the tumor removal. IFN-γ, IL- 4, IL-10 and TGF-β mRNA levels in splenic cells were assessed by quantitative real-time RT-PCR. Cytotoxic activity of splenocytes was estimated by flow cytometry. We found that in splenocytes of mice received opioid analgesia IL-10 mRNA level was increased 2.3 times on day one after the surgery compared to preoperative level (P 0.05), while in dexketoprofen group this parameter did not change. IFN-γ gene expression level on day 3 after tumor removal was 40% higher in splenocytes of dexketoprofen treated mice as compared with omnopon treated animals (P 0.05). Cytotoxic activity of splenocytes on day 3 postsurgery was (62.2 ± 2.4)% in dexketoprofen against (50.2 ± 3.3)% in omnopon group. In conclusion, perioperative analgesia with cyclooxygenase inhibitor dexketoprofen in contrast to opioid analgesia with omnopon preserves higher functional activity of murine immune cells in the experimental model of tumor surgery Доп.точки доступа: Sydor, R. I. Khranovska, N. M. Skachkova, O. V. Skivka, L. M.