Вид документа : Статья из журнала Шифр издания : Автор(ы) : Myronovskij S. L., Boiko N. M., Chumak V. V., Shorobura M. S., Lootsyk M. D., Stoika R. S., Kit Yu. Ya. Заглавие : The characteristics of antibodies of mice immunized by human unconventional myosin 1c Параллельн. заглавия :Характеристика антитіл мишей, імунізованих неконвенційним міозином людини Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 6. - С. 63-69 (Шифр УУ1/2016/88/6) Примечания : Bibliogr. at the end of the art. MeSH-главная: МОДЕЛИ НА ЖИВОТНЫХ -- MODELS, ANIMAL МИОЗИНЫ -- MYOSINS АНТИГЕН-АНТИТЕЛО РЕАКЦИИ -- ANTIGEN-ANTIBODY REACTIONS АСЦИТИЧЕСКАЯ ЖИДКОСТЬ -- ASCITIC FLUID АУТОАНТИТЕЛА -- AUTOANTIBODIES Аннотация: Specific antibodies produced against a protein of interest are invaluable tools for monitoring the protein structure, intracellular location and biological activity. Inoculation of murine lymphoma cells into the peritoneal cavity of immunized mice provides generation of ascitic fluid containing a significant amount of antibody with desired antigen specificity. Here we demonstrated that the intraperitoneal administration of murine lymphoma NK/Ly cells in mice immunized with 48 kDa isoform of human blood serum unconventional myosin 1c leads to generation of ascitic fluid that contained specific IgG-antibodies. These antibodies were capable of binding of the unconventional myosin 1c isolated from blood serum of patients with multiple sclerosis, rheumatoid arthritis and systemic lupus erythematosis, and could be used for diagnostics of several autoimmune diseases, the multiple sclerosis in particular Доп.точки доступа: Myronovskij, S. L. Boiko, N. M. Chumak, V. V. Shorobura, M. S. Lootsyk, M. D. Stoika, R. S. Kit, Yu. Ya.