Вид документа : Статья из журнала Шифр издания : Автор(ы) : Veklich T. O., Skrabak O. A., Nikonishyna Yu. V., Rodik R. V., Kalchenko V. I., Kosterin S. O. Заглавие : Calix[4]arene C-956 selectively inhibits plasma membrane Ca2+,Mg2+-ATPase in myometrial cells Место публикации : The Ukrainian Biochemical Journal. - Київ, 2018. - Том 90, N 5. - С. 34-42 (Шифр УУ60/2018/90/5) MeSH-главная: МИОМЕТРИЙ -- MYOMETRIUM Аннотация: Using enzymatic assays and kinetic analysis, we demonstrated that 100 µM calix[4]arene C-956 (5,11,17,23-tetra(trifluoro)methyl-(phenylsulfonylimino) methylamino-25,27-dioctyloxy-26,28-dipropoxycalix[4]arene) had the most significant inhibitory effect on the plasma membrane Са2+,Mg2+-АТРase activity compared to effects of other calix[4]arenes, and had no effect on specific activities of other membrane ATPases. Using confocal microscopy and fluorescent probe fluo-4, we observed an increase of the intracellular level of Ca2+ after application of calix[4]arene C-956 to immobilized myocytes. Analysis of the effect of calix[4]arene C-956 on the hydrodynamic diameter of myocytes demonstrated that application of calix[4]arene C-956 solution decreased this parameter by 45.5 ± 9.4% compared to control value similarly to the action of uterotonic drug oxytocin Доп.точки доступа: Veklich, T. O. Skrabak, O. A. Nikonishyna, Yu. V. Rodik, R. V. Kalchenko, V. I. Kosterin, S. O.