Вид документа : Статья из журнала Шифр издания : Автор(ы) : Kolupaev Yu. E., Horielova E. I., Yastreb T. O., Popov Yu. V., Ryabchun N. I. Заглавие : Phenylalanine ammonia-lyase activity and content of flavonoid compounds in wheat seedlings at the action of hypothermia and hydrogen sulfide donor Место публикации : The Ukrainian Biochemical Journal. - Київ, 2018. - Том 90, N 6. - С. 12-20 (Шифр УУ60/2018/90/6) MeSH-главная: ИММУНИТЕТ РАСТЕНИЙ -- PLANT IMMUNITY ГИПОТЕРМИЯ -- HYPOTHERMIA СЕРОВОДОРОД -- HYDROGEN SULFIDE ПШЕНИЦА -- TRITICUM Аннотация: At present hydrogen sulfide (H2S) is considered as one of the signal mediators in plant cells. However, its role in formation of plant resistance to low temperatures and, in particular, in regulation of secondary metabolism under stress conditions remains poorly understood. The influence of H2S donor sodium hydrosulfide (NaHS) on phenylalanine ammonia-lyase (PAL) activity and content of flavonoids in wheat seedlings at normal temperature (21 °C) and under cold hardening conditions (7 days at 3 °C) was studied. After 2 days of the hardening temperature, a transient increase in PAL activity was noted. Also, activity of the enzyme was increased by treatment of plants with 0.1 or 0.5 mM NaHS under normal temperature conditions and especially at the background of cold hardening. By themselves, the cold hardening and the action of H2S donor caused an increase in total content of flavonoids and amount of anthocyanins. With the combination of hypothermia and treatment of seedlings with NaHS, this effect enlarged and the total content of flavonoids increased by 3.8, and anthocyanins increased by 1.8 times in comparison to the control. Treatment with the H2S donor caused a decrease in content of the lipid peroxidation product malonic dialdehyde in seedlings after the action of hardening temperature, and especially after their freezing at –5 °C. Also, under the influence of NaHS, survival of hardened and unhardened seedlings after cryostress increased. It was concluded that one of the mechanisms of the positive influence of the H2S donor on resistance of wheat seedlings to hypothermia is the PAL-dependent accumulation of flavonoid compounds, which have a high antioxidant activity, and a decrease in effects of secondary oxidative stress Доп.точки доступа: Kolupaev, Yu. E. Horielova, E. I. Yastreb, T. O. Popov, Yu. V. Ryabchun, N. I.