Вид документа : Статья из журнала Шифр издания : Автор(ы) : Studennikov A. E., Glushkov A. N., Ustinov V. A. Заглавие : Detection of antibodies against benzo[A]pyrene in blood sera of lung cancer patients by ELISA using human recombinant idiotypic and anti-idiotypic antibodies Место публикации : Experimental Oncology. - К., 2021. - Том 43, N 4. - С. 323-327 (Шифр ЕУ12/2021/43/4) MeSH-главная: ЛЕГКИХ НОВООБРАЗОВАНИЯ -- LUNG NEOPLASMS ИММУНОФЕРМЕНТНЫЕ МЕТОДЫ -- IMMUNOENZYME TECHNIQUES Аннотация: Aim of this study was to develop and apply an ELISA method for assessment of the levels of endogenous antibodies against benzo[a]pyrene (Bp) in blood sera of lung cancer (LC) patients. Materials and Methods: ELISA was developed and applied for the detection of endogenous idiotypic and anti-idiotypic antibodies against Bp in human blood sera using recombinant human idiotypic and anti-idiotypic antibodies against Bp. Results: Serum samples of LC patients (n = 22) and healthy donors (n = 24) were analyzed by the new method. Statistical analysis showed that in sera of LC patients the levels of endogenous idiotypic and anti-idiotypic antibodies against Bp were significantly higher than in healthy donors. A logistic regression model for the LC detection utilizing such predictors as the serum levels of idiotypic and anti-idiotypic antibodies against Bp, smoking status, and age, identified LC patients with 83% specificity and 82% sensitivity. Conclusion: The proposed method could be further developed as additional lung cancer screening tool Доп.точки доступа: Glushkov, A. N. Ustinov, V. A.