Вид документа : Статья из журнала Шифр издания : Автор(ы) : Petrellis M. C., Frigo L., Ribeiro W., Leal-Junior E. C. P., Oliveira F. R., Maria D. A., Lopes-Martins R. Á. B. Заглавие : Proinflammatory effects of photoactivated methylene blue on rat model of Walker 256 carcinosarcoma Место публикации : Экспериментальная онкология. - 2019. - Т. 41, № 2. - С. 112-122 (Шифр ЕУ12/2019/41/2) Примечания : Bibliogr. at the end of the art. Аннотация: Photodynamic therapy (PDT) is an anticancer therapy that associates the photosensitizer (PS), oxygen and light to destroy cancer cells. Methylene blue (MB) is considered a second generation phenothiazine dye with excellent photochemical properties. Aim: To evaluate whether MB-mediated PDT can induce oxidative stress and inflammation, therefore, interfering tumor growth. Materials and Methods: The study was conducted on Wistar rats transplanted with Walker 256 carcinosarcoma (W256). The proinflammatory interleukins levels (IL-1β, IL-6, IL-10, TNF-α) were determined by ELISA, mRNA expression of COX-1, COX-2, iNOS and eNOS by RT-PCR, lipid peroxidation was measured by the TBARS method. Moreover, myeloperoxidase (MPO) activity in neutrophils was determined by MPO activity assay. All indices mentioned above were determined in tumor tissue. Kaplan — Meier and Gehan — Breslow — Wilcoxon tests were used for survival analysis. Results: We found that the treatment of W256 with 0.1% MB + 1 J/cm2 provoked a significant increase in the interleukins levels (IL-1β, IL-6, IL-10, TNF-α), prostaglandin E2, the mRNA expression of COX-2, iNOS, lipid peroxidation and MPO activity in tumor tissue, which were statistically different (p 0.05) compared to other experimental and control groups. The results of the estimation of survival curves show a greater probability of survival in 0.1% MB + 1 J/cm2 (total energy dose Доп.точки доступа: Petrellis, M. C. Frigo, L. Ribeiro, W. Leal-Junior, E. C. P. Oliveira, F. R. Maria, D. A. Lopes-Martins, R. Á. B.