Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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1-10 11-20 21-30 31-36

Type of document : Magazine article
Edition cipher :
Author(s) : Chekhun V. F.
Title : On the 40th anniversary of the international symposium “The role of stem cells in leukemo-and carcinogenesis»: in Kyiv again
Place of publication : Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 162-163 (Cipher ЕУ12/2017/39/3)
MeSH-main: ЮБИЛЕИ И ДРУГИЕ ВАЖНЫЕ СОБЫТИЯ -- ANNIVERSARIES AND SPECIAL EVENTS
СЪЕЗДЫ, СИМПОЗИУМЫ -- CONVENTIONS, SYMPOSIUMS
СТВОЛОВЫЕ КЛЕТКИ -- STEM CELLS
НЕОПЛАСТИЧЕСКИЕ ПРОЦЕССЫ -- NEOPLASTIC PROCESSES
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Type of document : Magazine article
Edition cipher :
Author(s) : Chekhun V. F., Lukianova N. Y., Chekhun S. V., Bezdieniezhnykh N. O., Zadvomiy T. V., Borikun T. V., Polishchuk L. Z., Klyusov O. M.
Title : Association of CD44⁺CD24^-/low with markers of aggressiveness and plasticity of cell lines and tumors of patients with breast cancer
Place of publication : Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 203-211 (Cipher ЕУ12/2017/39/3)
Notes : Bibliogr. at the end of the art.
MeSH-main: МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS
НОВООБРАЗОВАНИЙ МАРКЕРЫ БИОЛОГИЧЕСКИЕ -- TUMOR MARKERS, BIOLOGICAL
ЦИТОСТАТИЧЕСКИЕ СРЕДСТВА -- CYTOSTATIC AGENTS
ЛЕКАРСТВЕННАЯ УСТОЙЧИВОСТЬ НОВООБРАЗОВАНИЙ -- DRUG RESISTANCE, NEOPLASM
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
Annotation: To search for additional molecular-biological markers of cancer stem cell (CSC) involved in the development of intra-tumor heterogeneity for the detection of features of the breast cancer (BC) pathogenesis. Materials and methods: Expression of estrogen receptors (ER), progesterone receptors (PR), Her2/neu, E- and N-cadherin, CD24, CD44, Bcl-2, Bax, Slug, P-gp, glutathione-S-transferase (GST) and metallothionein in cell lines was determined by the immunocytochemical method. Expression of ER, PR, Her2/neu, CD24 and CD44 in the surgical material of BC patients were determined by the immunohistochemical method. The levels of the miRNA were determined using real-time polymerase chain reaction. Results: Cells of high-grade malignancy (HGM), MDA-MB-231 and MDA-MB-468 are characterized by high expression of stem cell markers compared to the cells of low-grade malignancy (LGM), T47D and MCF-7: CD44 levels in T47D and MCF-7 cells were in range of 72-79 points, which is significantly lower than in HGM cells (p 0.05). Also, HGM cells with the properties of CSC were characterized by high expression of antiapoptotic proteins, the transcription factor Slug, and low levels of proapoptotic protein Bax (p 0.05) compared to LGM cells. In cells with CSC characteristics an increased expression of transferrin and its receptor, ferritin, fentorin and hepcidin was revealed indicating activation of the endogenous iron metabolism. The characteristic feature of HGM cells with CSC phenotype were the increased levels of oncogenic miR-221, -155 and -10b by 60%, 92% and 78%, respectively, and decreased levels of oncosuppressive miR-29b, -34a and -200b by 8.4 ± 0.3, 4.6 ± 0.2, and 3.4 ± 0.6 times compared to MCF-7 line cells. It has been established that the development of resistance to cytostatics is accompanied by increased aggressiveness of tumor cells, loss of expression of hormonal receptors and acquiring of stem phenotype. In particular, increased expression of P-gp was observed in BC cells during the development of resistance to doxorubicin, of GST during the development of resistance to cisplatin along with increased CD44 expression (p 0.05). We have revealed the relation between the presence of cells with the CSC phenotype (CD44+CD24-/low) and clinical and pathological characteristics of BC patients, their survival and BC sensitivity to neoadjuvant therapy (p > 0.05). Conclusions: The dependence between the expression of CSC markers and the degree of malignancy of tumor cells, development of resistance to cytostatics in vitro was established as well as the predictive value of the detection of the CSC for the individual prognosis of the BC course and sensitivity of the tumors to the treatment
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Type of document : Magazine article
Edition cipher :
Author(s) : Boroday N. V., Chekhun V. F.
Title : Morphological features of doxorubicin-resistant Walker 256 carcinosarcoma and response of mast cells
Place of publication : Экспериментальная онкология. - 2018. - Т. 40, № 1. - С. 42-47 (Cipher ЕУ12/2018/40/1)
Notes : Bibliogr. at the end of the art.
MeSH-main: КАРЦИНОМА 256 УОКЕРА -- CARCINOMA 256, WALKER
ЛЕКАРСТВЕННАЯ УСТОЙЧИВОСТЬ НОВООБРАЗОВАНИЙ -- DRUG RESISTANCE, NEOPLASM
ТУЧНЫЕ КЛЕТКИ -- MAST CELLS
ДОКСОРУБИЦИН -- DOXORUBICIN
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS
Annotation: The mechanisms of drug resistance of cancer have not been yet elucidated in details. Recently, the role of mast cells (MCs) in the development of drug resistance has been brought in the limelight. The aim of the study was to examine the morphological features of doxorubicin (DOX)-resistant Walker 256 carcinosarcoma and to assess the response of MCs and histamine content in these cells in relation to the development of resistance to DOX as well as in DOX-resistant tumors. Materials and Methods: The DOX resistance was induced by serial passages of Walker 256 carcinosarcoma in rats in the setting of DOX treatment in vivo. MCs in tumors were detected in the sections by staining with Toluidine Blue O. Histamine content in MCs stained with solution of Water Blue-Orcein was assessed by Astaldi semiquantitative method taking into account different staining intensity. Results: Formation of DOX resistance in the course of serial passages of Walker 256 carcinosarcoma was accompanied by the increase in the number of MCs in tumors and histamine content. Nevertheless, in tumors with phenotype of complete DOX resistance the number of histamine-containing MCs decreased to the same level as in tumors of the original strain that are DOX-sensitive. Conclusion: MCs are involved in formation of DOX resistance in Walker 256 carcinosarcoma
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Type of document : Magazine article
Edition cipher :
Author(s) : Chumak A. V., Fedosova N.I., Cheremshenko N. L., Symchych T. V., Voyeykova I. M., Chekhun V. F.
Title : Macrophage polarization in dynamics of Lewis lung carcinoma growth and metastasis
Parallel titles :Зміни поляризації макрофагів у динаміці росту метастазуючої карциноми Льюїс
Place of publication : Экспериментальная онкология. - 2021. - Т. 43, № 1. - С. 15-20 (Cipher ЕУ12/2021/43/1)
Notes : Bibliogr. at the end of the art.
MeSH-main: КАРЦИНОМА ЛЬЮИС ЛЕГКОГО -- CARCINOMA, LEWIS LUNG
НОВООБРАЗОВАНИЙ МЕТАСТАЗЫ -- NEOPLASM METASTASIS
МОДЕЛИ НА ЖИВОТНЫХ -- MODELS, ANIMAL
Annotation: To assess the functional state of macrophages based on various manifestations of their activity at the different stages of metastatic tumor growth in C57Bl mice. Materials and Methods: On days 7, 14, 21 and 28 after Lewis lung carcinoma transplantation to C57Bl mice, macrophages from various anatomic sites were isolated and tested on their cytotoxicity, metabolic activity, NO production and arginase activity. Results: In the populations of peritoneal and splenic macrophages, on days 7 and 21 of tumor growth antitumor (M1) cells prevailed while on days 14 and 28 tumor-promoting (M2) macrophages predominated. In the population of lung macrophages, cells with M1 phenotype were in the majority in the early stages of tumor growth. On days 21 and 28, M1 cells were gradually substituted by cells exhibiting M2 phenotype. This shift correlated with metastasis to lungs. Conclusion: Lewis lung carcinoma growth is accompanied by the gradual change in macrophage polarization from antitumor (M1) towards tumor-promoting (M2) type. These changes were more evident in population of lung macrophages and correlated with the parameters of metastasisОцінити стан макрофагів мишей лінії С57Вl за різними проявами їх функціональної активності на різних стадіях росту метастазуючої пухлини. Матеріали та методи: У дослідженні використовували мишей лінії C57Bl з епідермоїдною метастазуючою карциномою легені Льюїс. На 7-, 14-, 21- та 28-й день після перещеплення пухлини макрофаги з різних ніш були виділені та піддані функціональному аналізу. Результати: Макрофаги, отримані з різних біологічних ніш у інтактних тварин, за дослідженими показниками функціональної активності мали фенотип М1. У популяціях макрофагів, отриманих з селезінки та перитонеальної порожнини мишей лінії С57Вl на 7-і 21-шу доби росту карциноми легені Льюїс переважали клітини з протипухлинними властивостями (М1), на 14- та 28-му добу — клітини з пропухлинними властивостями (М2). У популяції легеневих макрофагів на ранніх стадіях пухлинного процесу переважали клітини з фенотипом М1, до 28-ї доби спостерігали поступову їх поляризацію до фенотипу М2. Такі зміни корелювали з показниками метастазування в легені в ці терміни. Висновок: Aналіз показників функціональної активності макрофагів свідчить про їх поступову поляризацію від клітин з проти- (М1) до клітин з пропухлинними (М2) властивостями в динаміці росту карциноми легені Льюїс. Виявлені зміни були найбільш вираженими в популяції легеневих макрофагів та мали кореляційний зв’язок з показниками метастазування
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Type of document : Magazine article
Edition cipher :
Author(s) : Buchynska L. G., Iurchenko N. P., Kashuba E. V., Brieieva O. V., Glushchenko N. M., Mints M., Lukianova N. Yu., Chekhun V. F.
Title : Overexpression of the mitochondrial ribosomal protein S 18-2 in the invasive breast carcinomas
Place of publication : Experimental Oncology. - К., 2018. - Том 40, N 4. - С. 303-308 (Cipher ЕУ12/2018/40/4)
MeSH-main: МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS
ВНУТРИКЛЕТОЧНЫЕ СИГНАЛЬНЫЕ ПЕПТИДЫ И БЕЛКИ -- INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS
Annotation: Recent studies allow to consider the mitochondrial ribosomal protein S18-2 (MRPS18-2, S18-2) as a potential oncoprotein, which suggests the need for further characterization of its expression in tumors of different genesis including breast cancer (BC). The aim of the study was to analyze the expression of the S18-2 protein in BC of luminal A and basal subtypes. Materials and Methods: Operational material of BC patients stage І–ІІ (luminal A subtype, n = 30, and basal subtype, n = 10) was studied with the use of morphological, immunohistochemical, statistical and bioinformatic methods. Results: Using the immunohistochemical analysis, we found that the S18-2 protein showed the nuclear signal in 66.7% of luminal A subtype BC samples and 80.0% of basal subtype BC samples. The variability of the S18-2 expression in both the luminal A and basal subtypes of BC was revealed. Noteworthy, the number of cells expressing S18-2 in high-proliferating tumors of luminal A and basal subtype is significantly higher than in tumors with a low proliferative potential (p 0.05). In 10 samples of luminal A subtype, the nuclear S18-2 signal was higher than median value. Moreover, the S18-2 protein was overexpressed in 4 out of such 10 samples. Metastases in the lymph nodes were found in 3 out of 4 patients with the stage II BC, low differentiation grade of the tumor and high proliferative activity. The bioinformatic analysis confirms our preliminary findings that the trend for increasing expression of the S18-2 protein in tumors correlates with the aggressiveness of malignant BC. Conclusion: The S18-2 protein may be a marker of cancer aggressiveness in BC patients
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Type of document : Magazine article
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Author(s) : Chumak A. V., Fedosova N. I., Shcherbina V. M., Cheremshenko N. L., Karaman O. M., Chekhun V. F.
Title : Influence of bacterial lectin on key regulatory links of functional activity of macrophages in mice with Ehrlich carcinoma
Place of publication : Experimental Oncology. - К., 2021. - Том 43, N 3. - С. 197-203 (Cipher ЕУ12/2021/43/3)
MeSH-main: КАРЦИНОМА, ЭРЛИХА ОПУХОЛЬ -- CARCINOMA, EHRLICH TUMOR
ИММУНОТЕРАПИЯ -- IMMUNOTHERAPY
МАКРОФАГИ -- MACROPHAGES
ЭУКАРИОТЫ -- EUKARYOTA
Annotation: Recent studies have shown the potential of using different approaches for immunotherapy in cancer treatment. Macrophages (Mph) are one of the promising targets for immunotherapy. Aim: To investigate changes in the functional activity of Mph in mice with Ehrlich carcinoma by nitric oxide (NO)/arginase (Arg), IRF4/IRF5 and STAT1/STAT6 ratios caused by administration of lectin from B. subtilis IMV-7724. Materials and Methods: From the 2nd day after Ehrlich carcinoma inoculation into female Balb/c mice, lectin from B. subtilis IMV B-7724 (0.02 mg/mouse) was administered for 10 days. The peritoneal Mph were isolated on days 14, 21, and 28 after tumor transplantation and their functional state (NO production, Arg activity and cytotoxic activity) was examined. The levels of mRNA expression of transcription factors STAT-1, STAT-6, IRF5, IRF4 were evaluated. Results: In lectin-treated animals with Ehrlich carcinoma, the functional state of Mph (NO/Arg ratio, index of cytotoxic activity) was maintained at the level of intact mice exceeding the values in untreated animals with Ehrlich carcinoma at late terms of tumor growth (21, 28 days). Analysis of mRNA expression levels of transcription factors in these animals showed a significant increase (p 0.05) in the ratio of STAT1/STAT6 on the day 21 and IRF5/IRF4 on day 28 of tumor growth compared to that in untreated mice. Conclusions: Administration of lectin from B. subtilis IMV B-7724 to mice with Ehrlich carcinoma led to the prevalence of Mph exhibiting the functional properties of M1 type at late-term tumor growth. The transcription factors of the STAT and IRF signaling pathways are involved in the process of Mph polarization induced by lectin from B. subtilis IMV B-7724
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Type of document : Magazine article
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Author(s) : Chekhun V. F., Domina E. A.
Title : Can SARS-CJV-2 change individual radiation sensitivity of the patients recovered from COVID-19? (experimental and theoretical backlground)
Place of publication : Experimental Oncology. - К., 2021. - Том 43, N 3. - С. 277-280 (Cipher ЕУ12/2021/43/3)
MeSH-main: КОРОНАВИРУСНЫЕ ИНФЕКЦИИ -- CORONAVIRUS INFECTIONS
РАДИОТОЛЕРАНТНОСТЬ -- RADIATION TOLERANCE
Annotation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the National Academy of Science of Ukraine has been studying the mechanisms and specificities of individual radiation sensitivity (IRS) formation in professionals who work in the field of ionizing radiation, cancer patients and representatives of other population groups. Our data based on the use of G2-test in in vitro irradiated blood lymphocytes in late G2-period of cell cycle indicated an increased carcinogenic risk in professionals with high IRS. We suggest that the COVID-19 pandemic could make significant adjustments in the formation of IRS in professionals who have survived the disease and continue to work with ionizing radiation (IR). Increased systemic inflammatory activity, which persists for a long time in COVID-19 patients, in combination with low-dose range irradiation (professionals who continue to work with IR) and with local irradiation in the high-dose range (radiation therapy for cancer patients) may affect IRS. Repeated determination of IRS in professionals who have had COVID-19 infection, using chromosomal G2-radiation sensitivity assay will answer the question: can SARS-CoV-2 coronavirus affect the IRS? The proposed hypothesis of the radiosensitivity evolution needs further experimental validation using a set of radiobiological indices to clarify the mechanism of IRS formation following COVID-19 infection. The detected changes (increase) of human IRS after COVID-19 must be taken into account for personalized planning of radiotherapy of COVID-19 cancer patients
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Type of document : Magazine article
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Author(s) : Lozovska Yu. V., Andrusishina I. M., Lukianova N. Yu., Burlaka A. P., Naleskina L. A., Todor I. N., Chekhun V. F.
Title : The influence of lactoferrin on elemental homeostasis and activity of metal-containing enzymes in rats with Walker-256 carcinosarcoma
Place of publication : Экспериментальная онкология. - 2019. - Т. 41, № 1. - С. 20-25 (Cipher ЕУ12/2019/41/1)
Notes : Bibliogr. at the end of the art.
Annotation: To investigate the content of essential elements (EE): copper, zinc, magnesium, iron and calcium and the evaluation of the activity of metal-containing enzymes — ceruloplasmin (CP), myeloperoxidase (MPO) and the content of transferrin (TF) in blood plasma (BP) and tumor tissue (TT) of animals with Walker-256 carcinosarcoma treated with lactoferrin (LF). Materials and Methods: The study of the EE content and the activity of the abovementioned enzymes was carried out on rats with Walker-256 carcinosarcoma treated with LF at the doses of 1 and 10 mg/kg of body weight. The quantitative content of EE in BP and TT of animals was determined using the inductively coupled plasma atomic emission spectroscopy (ICP-AES). Determination of CP activity, content of TF and hemochromes was performed using the method of electron paramagnetic resonance (EPR), and MPO — by unified biochemical method. Results: The introduction of LF at the doses of 1 and 10 mg/kg resulted in a decrease in the ratio of Cu/Zn in BP and even more expressed decrease of Ca/Mg ratio in TT. Administration of LF, especially at a dose of 10 mg/kg, affected the increase in CP and MPO activity in BP. It has been shown that administration of LF at a dose of 10 mg/kg led to an increase in oxidative products of destruction of the hemoglobin-hemochrom system in the TT, against the background of lowering the TF content. Conclusions: The administration of LF, especially at a dose of 10 mg/kg, led to metabolic alterations associated with inhibition of the tumor process. The detected modulating effect of LF on the content of the EE and the activity of the CP and MPO may be a basis for correction of the elemental balance in carcinogenesis
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Type of document : Magazine article
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Author(s) : Chekhun V. F.
Title : MicroRNAs are a key factor in the globalization of tumor-host relationships
Place of publication : Экспериментальная онкология. - 2019. - Т. 41, № 3. - С. 188-194 (Cipher ЕУ12/2019/41/3)
Notes : Bibliogr. at the end of the art.
Annotation: A new round of molecular oncology development in the post-genomic era significantly changes the conventional understanding of the nature and origin of the malignant process. Increasingly, fundamental phenomena are emerging that change the “canonical” views of the dominant role of gene mutations that contribute to the uncontrolled proliferation and emergence of heterogeneous malignant cells. Recent numerous studies have shown that significant variability in the initiation, proliferation and control of the apoptotic program of tumor cells is due to numerous epigenetic processes, including the level of expression of microRNAs (miRNAs). This paper reviews the literature data and presents the results of own research in which miRNAs have been found to form a molecular phenotype for malignancy. Their role as a “conductor” of the functioning of a genetic-epigenetic orchestra that coordinates various aspects of malignancy has been suggested. MiRNAs have been shown to be an active participant in balancing mechanisms in the development of controversial processes in breast cancer cell lines of varying degrees of malignancy. The analysis of the literature data and the own studies of the expression profile of only a few miRNAs suggests that scientists and clinicians have received a new marker and a target that simultaneously plays the role of an active insider in both the oncogene-oncosuppressor relationship and in the globalization of this process at the tumor-host level. Further investigation of the “alarm” marker in this network will allow to reconsider the molecular genetic classification of neoplastic disease, which will provide the development of a new strategy for cancer therapy
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10.

Type of document : Magazine article
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Author(s) : Lukianova N. Yu., Borikun T.V., Chekhun V. F.
Title : Tumor microenvironment-derived miRNAs as prognostic markers of breast cancer
Place of publication : Экспериментальная онкология. - 2019. - Т. 41, № 3. - С. 242-247 (Cipher ЕУ12/2019/41/3)
Notes : Bibliogr. at the end of the art.
Annotation: To study expression of miRNA derived from tumor microenvironment in patients with breast cancer (BC) as the aspect of tumor-host interaction. Materials and Methods: The expression levels of estrogen receptor, progesterone receptor, human epidermal growth factor receptor 2 (HER2/neu) were analyzed in tissue of BC using immunohistochemical method. Relative expression levels of the miR-155, -320a, and -205 were examined in tissue and sera from BC patients using quantitative reverse transcription polymerase chain reaction. Results: Serum and tissue miR-155, -320a, and -205 levels in patients with BC are of low diagnostic value as such for differentiation of malignant and non-malignant breast neoplasms. Nevertheless, we established the relation of circulating and tissue miR-155, -320a, and -205 to lymph node metastases and basal breast cancer subtype. Conclusions: Changes of miR-155, -320a, and -205 expression in tumor tissue and sera of BC patients provide information about major clinical-pathological characteristics of BC
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