Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

Home page

Simplified mode

Video-instruction

Description

Data bases

Periodicals- results of search

Type of search

Periodicals

Search field

in the found

Found in other databases:

Books (36)

Local Lore (5)

Rare Editions (22)

the format of presenting the found documents:
full	informational	short

Sort out the found documents by:
to the author	by title	Year of publication	document type

Search inquiry: <.>DP=202407$<.>

Total number of found documents : 129
Shown documents from 1 till 10

1-10 11-20 21-30 31-40 41-50 51-60

Cipher: ЕУ12/2017/39/3
Journal

Экспериментальная онкология [Text]
2017year Т. 39 № 3 . - 34.76, р.

Contents:
Chekhun, V. F. On the 40th anniversary of the international symposium “The role of stem cells in leukemo-and carcinogenesis»: in Kyiv again / V. F. Chekhun. - С.162-163
Kalynychenko, T. O. Umbilical cord blood banking in the worldwide hematopoietic stem cell transplantation system: perspectives for Ukraine / T. O. Kalynychenko. - С.164-170. - Bibliogr. at the end of the art.
Vasyliev, R. G. Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications / R. G. Vasyliev [та ін.]. - С.171-180. - Bibliogr. at the end of the art.
Other authors: Rodnichenko A. E., Gubar O. S., Zlatska A. V., Gordiienko I. M., Novikova S. N., Zubov D. O.
Kozub, M. M. Comparison of various tissue and cell therapy approaches when restoring ovarian, hepatic and kidney’s function after chemotherapy-induced ovarian failure / M. M. Kozub [та ін.]. - С.181-185. - Bibliogr. at the end of the art.
Other authors: Prokopiuk V. Y., Skibina K. R., Prokopiuk О. V., Kozub N. I.
Gubar, O. S. Postnatal extra-embryonic tissues as a source of multiple cell types for regenerative medicine applications / O. S. Gubar [та ін.]. - С.186-190. - Bibliogr. at the end of the art.
Other authors: Rodnichenko A. E., Vasyliev R. G., Zlatska A. V., Zubov D. O.
Vasyliev, R. G. Tissue-engineered bone for treatment of combat related limb injuries / R. G. Vasyliev [та ін.]. - С.191-196. - Bibliogr. at the end of the art.
Other authors: Oksymets V. M., Rodnichenko A. E., Zlatska A. V., Gubar O. S., Gordiienko I. M., Zubov D. O.
Zlatska, A. V. Endometrial stromal cells: isolation, expansion, morphological and functional properties / A. V. Zlatska [та ін.]. - С.197-202. - Bibliogr. at the end of the art.
Other authors: Rodnichenko A. E., Gubar O. S., Zubov D. O., Novikova S. N., Vasyliev R. G.
Chekhun, V. F. Association of CD44⁺CD24^-/low with markers of aggressiveness and plasticity of cell lines and tumors of patients with breast cancer / V. F. Chekhun [та ін.]. - С.203-211. - Bibliogr. at the end of the art.
Other authors: Lukianova N. Y., Chekhun S. V., Bezdieniezhnykh N. O., Zadvomiy T. V., Borikun T. V., Polishchuk L. Z., Klyusov O. M.
Karlitepe, A. Anti-cancer efficiency of natural killer cells differentiated from human adipose tissue-derived mesenchymal stem cells and transfected with miRNA150 / A. Karlitepe [та ін.]. - С.212-218. - Bibliogr. at the end of the art.
Other authors: Kabadayi H., Vatansever S., Gurdal M., Gunduz C., Ercan G.
Lisyaniy, N. I. Content of stem tumor CD133⁺ cells in brain neoplasms of different histological type / N. I. Lisyaniy [та ін.]. - С.219-223. - Bibliogr. at the end of the art.
Other authors: Stanetskaya D. N., Lisyaniy A. N., Belskaya L. N.
Ryspayeva, D. E. Are CD44⁺CD24^- cells the assumed cancer stem cells in breast cancer? / D. E. Ryspayeva [та ін.]. - С.224-228. - Bibliogr. at the end of the art.
Other authors: Smolanka I. I., Dudnichenko A. S., Lyashenko A. A., Grinevich Yu. A., Gurianov V. G., Koshubarova M. V., Seleznev A. A.
Bezdieniezhnykh, N. O. Scientific-practical and legal problems of implementation of the personalized medicine / N. O. Bezdieniezhnykh, V. V. Reznikova, O. V. Rossylna. - С.229-233
Normal and cancer stem cells: discovery diagnosis and therapy international scientific conference. R. E. Kavetsky Institute of Experimental Pathology Oncology and Radiobiology National Academy of Sciences of Ukraine Kyiv October 5–6 2017. - С.234-256
The copies are in the departments: total 1
Free: 1

Find similar
Follow to the descriptions of the articles

Chekhun, V. F.
On the 40th anniversary of the international symposium “The role of stem cells in leukemo-and carcinogenesis»: in Kyiv again [Text] / V. F. Chekhun // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 162-163

MeSH-main:
ЮБИЛЕИ И ДРУГИЕ ВАЖНЫЕ СОБЫТИЯ -- ANNIVERSARIES AND SPECIAL EVENTS
СЪЕЗДЫ, СИМПОЗИУМЫ -- CONVENTIONS, SYMPOSIUMS
СТВОЛОВЫЕ КЛЕТКИ -- STEM CELLS
НЕОПЛАСТИЧЕСКИЕ ПРОЦЕССЫ -- NEOPLASTIC PROCESSES
There are no free copies

Find similar

Kalynychenko, T. O.
Umbilical cord blood banking in the worldwide hematopoietic stem cell transplantation system: perspectives for Ukraine [Text] / T. O. Kalynychenko // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 164-170. - Bibliogr. at the end of the art.

MeSH-main:
БЕЛКИ КРОВИ -- BLOOD PROTEINS (анализ, снабжение и распределение)
ПЛОДА КРОВЬ -- FETAL BLOOD (трансплантация)
КРОВЕТВОРНЫЕ СТВОЛОВЫЕ КЛЕТКИ -- HEMATOPOIETIC STEM CELLS
ТЕХНОЛОГИЙ РАСПРОСТРАНЕНИЕ -- TECHNOLOGY TRANSFER
Annotation: Significant progress in the promotion of procedural technologies associated with the transplantation of hematopoietic stem cells caused a rapid increase in activity. The exchange of hematopoietic stem cells for unrelated donor transplantations is now much easier due to the relevant international professional structures and organizations established to support cooperation and standard setting, as well as rules for the functioning of both national donor registries and cord blood banks. These processes are increasing every year and are contributing to the outpacing rates of development in this area. Products within their country should be regulated by the competent government authorities. This study analyzes the work of international and national levels of support for transplantation activity in the field of unrelated hematopoietic stem cell transplantation, the standardization order of technologies, as well as data that justify the need to create a network of donated umbilical cord blood banks in Ukraine as a factor in the development of allogeneic transplantation. This will promote the accessibility of international standards for the treatment of serious diseases for Ukrainian citizens
There are no free copies

Find similar

Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications [Text] / R. G. Vasyliev [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 171-180. - Bibliogr. at the end of the art.

MeSH-main:
СТВОЛОВЫХ КЛЕТОК ИССЛЕДОВАНИЕ -- STEM CELL RESEARCH
СТРОМАЛЬНЫЕ КЛЕТКИ МЕЗЕНХИМНЫЕ -- MESENCHYMAL STROMAL CELLS (физиология)
НЕРВНЫЙ ГРЕБЕШОК -- NEURAL CREST (ультраструктура, физиология)
ВОЛОСЯНОЙ ФОЛЛИКУЛ -- HAIR FOLLICLE (анатомия и гистология, ультраструктура)
ЧЕЛОВЕК -- HUMANS (анатомия и гистология)
ЦИТОФЛЮОРОМЕТРИЯ -- FLOW CYTOMETRY (использование, тенденции)
МОРФОЛОГИЧЕСКИЕ И МИКРОСКОПИЧЕСКИЕ ПОКАЗАТЕЛИ -- MORPHOLOGICAL AND MICROSCOPIC FINDINGS
ФЕНОТИП -- PHENOTYPE
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS
Annotation: The purpose of this work was to obtain, multiply and characterize the adult neural crest-derived multipotent stem cells from human hair follicle for their further clinical use. Materials and Methods: Adult neural crest-derived multipotent stem cells were obtained from human hair follicle by explant method and were expanded at large-scale up to a clinically significant number. The resulted cell cultures were examined by flow cytometry and immunocytochemical analysis. Their clonogenic potential, ability to self-renewal and directed multilineage differentiation were also investigated. Results: Cell cultures were obtained from explants of adult human hair follicles. Resulted cells according to morphological, phenotypic and functional criteria satisfied the definition of neural crest-derived multipotent stem cells. They had the phenotype Sox2⁺Sox10⁺Nestin⁺CD73⁺CD90⁺CD105⁺CD140a⁺CD 140b⁺CD146⁺CD166⁺CD271⁺CD349⁺ CD34⁻CD45⁻CD56⁻HLA⁻DR⁻, showed high clonogenic potential, ability to self-renewal and directed differentiation into the main derivatives of the neural crest: neurons, Schwann cells, adipocytes and osteoblasts. Conclusion: The possibility of a large-scale expansion of adult neural crest-derived multipotent stem cells up to 40–200·106 cells from minimal number of hair follicles with retention of their phenotype and functional properties are the significant step towards their translation into the clinical practice
Additional Access Points:
Vasyliev, R. G.
Rodnichenko, A. E.
Gubar, O. S.
Zlatska, A. V.
Gordiienko, I. M.
Novikova, S. N.
Zubov, D. O.

There are no free copies

Find similar

Comparison of various tissue and cell therapy approaches when restoring ovarian, hepatic and kidney’s function after chemotherapy-induced ovarian failure [Text] / M. M. Kozub [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 181-185. - Bibliogr. at the end of the art.

MeSH-main:
БОЛЕЗНЬ, МОДЕЛИ НА ЖИВОТНЫХ -- DISEASE MODELS, ANIMAL
ПЕРВИЧНАЯ ЯИЧНИКОВАЯ НЕДОСТАТОЧНОСТЬ -- PRIMARY OVARIAN INSUFFICIENCY (патофизиология, химически вызванный, этиология)
КЛЕТОЧНАЯ И ТКАНЕВАЯ ТЕРАПИЯ -- CELL- AND TISSUE-BASED THERAPY (использование, статистика, тенденции)
СТВОЛОВЫХ КЛЕТОК МЕЗЕНХИМНЫХ ТРАНСПЛАНТАЦИЯ -- MESENCHYMAL STEM CELL TRANSPLANTATION (использование, методы, статистика, тенденции)
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS
Annotation: About 1% of women suffer from premature ovarian failure, which leads to a significant deterioration in the life quality. Most often this condition is caused by performed chemotherapy, autoimmune diseases, surgery performed on ovaries, uterus, or fallopian tubes. The aim of this study was to compare different approaches of tissue and cell therapy in restoring the sexual function in case of ovarian failure induced by chemotherapy. Materials and Methods: The study was carried out in BALB/c mice with the modeled ovarian failure, induced by cyclophosphamide and busulfan. The restoration dynamics of ovarian and sexual function, liver and kidneys after application of cryopreserved explants, cryoextract, placental mesenchymal stem cells, those of adipose tissue has been studied. Results: It has been shown that the use of various methods of cell and tissue therapy has comparable efficacy when treating an ovarian failure induced by chemotherapy. The most rapid and complete restoration of the reproductive system, liver and kidneys was observed when using the placental explants, extract and cells, but not with the use of mesenchymal stem cells of adipose tissue. No recovery of fertility in this experiment was observed. Conclusion: Various methods of cellular and tissue therapy are perspective in treatment of the chemotherapy complications. More effective are placental derivates
Additional Access Points:
Kozub, M. M.
Prokopiuk, V. Y.
Skibina, K. R.
Prokopiuk, О. V.
Kozub, N. I.

There are no free copies

Find similar

Postnatal extra-embryonic tissues as a source of multiple cell types for regenerative medicine applications [Text] / O. S. Gubar [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 186-190. - Bibliogr. at the end of the art.

MeSH-main:
СТРОМАЛЬНЫЕ КЛЕТКИ МЕЗЕНХИМНЫЕ -- MESENCHYMAL STROMAL CELLS (ультраструктура)
ХОРИОНАЛЬНЫЕ ВОРСИНКИ -- CHORIONIC VILLI (эмбриология)
ПЛОДА КРОВЬ -- FETAL BLOOD
АЛЛОТРАНСПЛАНТАЦИЯ -- TRANSPLANTATION, HOMOLOGOUS (использование, методы, тенденции)
ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
Annotation: We aimed to isolate and characterize the cell types which could be obtained from postnatal extra-embryonic tissues. Materials and Methods: Fresh tissues (no more than 12 h after delivery) were used for enzymatic or explants methods of cell isolation. Obtained cultures were further maintained at 5% oxygen. At P3 cell phenotype was assessed by fluorescence-activated cell sorting, population doubling time was calculated and the multilineage differentiation assay was performed. Results: We have isolated multiple cell types from postnatal tissues. Namely, placental mesenchymal stromal cells from placenta chorionic disc, chorionic membrane mesenchymal stromal cells (ChM-MSC) from free chorionic membrane, umbilical cord MSC (UC-MSC) from whole umbilical cord, human umbilical vein endothelial cells (HUVEC) from umbilical vein, amniotic epithelial cells (AEC) and amniotic MSC (AMSC) from amniotic membrane. All isolated cell types displayed high proliferation rate together with the typical MSC phenotype: CD73⁺CD90⁺CD105⁺CD146⁺CD166⁺CD34⁻CD45⁻HLA⁻DR⁻. HUVEC constitutively expressed key markers CD31 and CD309. Most MSC and AEC were capable of osteogenic and adipogenic differentiation. Conclusion: We have shown that a wide variety of cell types can be easily isolated from extra-embryonic tissues and expanded ex vivo for regenerative medicine applications. These cells possess typical MSC properties and can be considered an alternative for adult MSC obtained from bone marrow or fat, especially for allogeneic use
Additional Access Points:
Gubar, O. S.
Rodnichenko, A. E.
Vasyliev, R. G.
Zlatska, A. V.
Zubov, D. O.

There are no free copies

Find similar

Tissue-engineered bone for treatment of combat related limb injuries [Text] / R. G. Vasyliev [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 191-196. - Bibliogr. at the end of the art.

MeSH-main:
СЛОЖНЫЕ ТКАНЕВЫЕ АЛЛОТРАНСПЛАНТАТЫ -- COMPOSITE TISSUE ALLOGRAFTS (трансплантация)
КОСТНОГО МОЗГА ТРАНСПЛАНТАЦИЯ -- BONE MARROW TRANSPLANTATION (методы, тенденции)
ВОЕННОСЛУЖАЩИЕ -- MILITARY PERSONNEL
ПЕРЕЛОМЫ КОСТИ -- FRACTURES, BONE (патофизиология, этиология)
СТВОЛОВЫХ КЛЕТОК МЕЗЕНХИМНЫХ ТРАНСПЛАНТАЦИЯ -- MESENCHYMAL STEM CELL TRANSPLANTATION (использование, методы, тенденции)
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS
Annotation: Based on our preliminary positive clinical results with use of cultured bone marrow-derived multipotent mesenchymal stem/stromal cells in traumatology, our aim was to develop living three-dimensional tissue-engineered bone equivalent transplantation technology for restoration of critical sized bone defects caused by combat related high energy trauma. Materials and Methods: To fabricate bone equivalent we used devitalized allogeneic bone scaffolds (blocks and chips) seeded with cultured autologous cells: bone marrow-derived multipotent mesenchymal stem/stromal cells in mix with periosteal progenitor cells and endothelial progenitor cells. Quality/identity of cell cultures was assured by donor and cell culture infection screening (immunofluorescence assay, polymerase chain reaction), flow cytometry (cell phenotype), karyotyping (GTG banding), functional assays (colony forming units analysis, multilineage differentiation assay). Bone defect treatment with bone equivalent application was fully completed in 39 combat-injured with 42 defects. New bone formation was assessed by the radiographic examination. Results: Casualties were included in a treatment program an average of 10.1 months after injury, provided the ineffectiveness of conventional surgery methods. All cell type cultures had a normal karyotype and appropriate phenotype, differentiation potential and functional properties, ~30% colony forming units frequency and hadn’t any signs of cell senescence. The fluorescein diacetate/propidium iodide combined staining and histology analysis of graft samples before transplantation showed their regular seeding with viable cells. Pathomorphological analysis of bone equivalent specimens 3–6 months post-op revealed the active remodeling processes and immature bone tissue formation. Bone defect restoration was observed 5–6 months post-op. Conclusion: The developed biotechnology of living three-dimensional tissue-engineered bone equivalent transplantation with overall effectiveness 90.4% allows restoring the bone integrity, forming new bone tissue in a site of bone defect, and significantly reducing the rehabilitation period of a patient
Additional Access Points:
Vasyliev, R. G.
Oksymets, V. M.
Rodnichenko, A. E.
Zlatska, A. V.
Gubar, O. S.
Gordiienko, I. M.
Zubov, D. O.

There are no free copies

Find similar

Endometrial stromal cells: isolation, expansion, morphological and functional properties [Text] / A. V. Zlatska [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 197-202. - Bibliogr. at the end of the art.

MeSH-main:
СТРОМАЛЬНЫЕ КЛЕТКИ -- STROMAL CELLS (ультраструктура, физиология)
IN VITRO МЕТОДЫ -- IN VITRO TECHNIQUES (использование, тенденции)
ЭНДОМЕТРИЙ -- ENDOMETRIUM (анатомия и гистология)
КОЛОНИЕОБРАЗУЮЩИХ ЕДИНИЦ АНАЛИЗ -- COLONY-FORMING UNITS ASSAY (методы, тенденции)
МЕДИЦИНА РЕГЕНЕРАТИВНАЯ -- REGENERATIVE MEDICINE (методы, организация и управление, тенденции)
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS
Annotation: We aimed to study biological properties of human endometrial stromal cells in vitro. Materials and methods: The endometrium samples (n = 5) were obtained by biopsy at the first phase of the menstrual cycle from women with endometrial hypoplasia. In all cases, a voluntary written informed consent was obtained from the patients. Endometrial fragments were dissociated by enzymatic treatment. The cells were cultured in DMEM/F12 supplemented with 10% FBS, 2 mМ L-glutamine and 1 ng/ml FGF-2 in a multi-gas incubator at 5% CO2 and 5% O2. At P3 the cells were subjected to immunophenotyping, multilineage differentiation, karyotype stability and colony forming efficiency. The cell secretome was assessed by BioRad Multiplex immunoassay kit. Results: Primary population of endometrial cells was heterogeneous and contained cells with fibroblast-like and epithelial-like morphology, but at P3 the majority of cell population had fibroblast-like morphology. The cells possessed typical for MSCs phenotype CD90+CD105+CD73+CD34-CD45-HLA-DR-. The cells also expressed CD140a, CD140b, CD146, and CD166 antigents; and were negative for CD106, CD184, CD271, and CD325. Cell doubling time was 29.6 ± 1.3 h. The cells were capable of directed osteogenic, adipogenic and chondrogenic differentiation. The cells showed 35.7% colony forming efficiency and a tendency to 3D spheroid formation. The GTG-banding assay confirmed the stability of eMSC karyotype during long-term culturing (up to P8). After 48 h incubation period in serum-free medium eMSC secreted anti-inflammatory IL-1ra, as well as IL-6, IL-8 and IFNγ, angiogenic factors VEGF, GM-CSF and FGF-2, chemokines IP-10 and MCP-1. Conclusion: Thus, cultured endometrial stromal cells meet minimal ISCT criteria for MSC. Proliferative potential, karyotype stability, multilineage plasticity and secretome profile make eMSC an attractive object for the regenerative medicine use
Additional Access Points:
Zlatska, A. V.
Rodnichenko, A. E.
Gubar, O. S.
Zubov, D. O.
Novikova, S. N.
Vasyliev, R. G.

There are no free copies

Find similar

Association of CD44⁺CD24^-/low with markers of aggressiveness and plasticity of cell lines and tumors of patients with breast cancer [Text] / V. F. Chekhun [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 203-211. - Bibliogr. at the end of the art.

MeSH-main:
МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS (осложнения, патофизиология, этиология)
НОВООБРАЗОВАНИЙ МАРКЕРЫ БИОЛОГИЧЕСКИЕ -- TUMOR MARKERS, BIOLOGICAL (анализ)
ЦИТОСТАТИЧЕСКИЕ СРЕДСТВА -- CYTOSTATIC AGENTS (анализ, терапевтическое применение)
ЛЕКАРСТВЕННАЯ УСТОЙЧИВОСТЬ НОВООБРАЗОВАНИЙ -- DRUG RESISTANCE, NEOPLASM (физиология)
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
Annotation: To search for additional molecular-biological markers of cancer stem cell (CSC) involved in the development of intra-tumor heterogeneity for the detection of features of the breast cancer (BC) pathogenesis. Materials and methods: Expression of estrogen receptors (ER), progesterone receptors (PR), Her2/neu, E- and N-cadherin, CD24, CD44, Bcl-2, Bax, Slug, P-gp, glutathione-S-transferase (GST) and metallothionein in cell lines was determined by the immunocytochemical method. Expression of ER, PR, Her2/neu, CD24 and CD44 in the surgical material of BC patients were determined by the immunohistochemical method. The levels of the miRNA were determined using real-time polymerase chain reaction. Results: Cells of high-grade malignancy (HGM), MDA-MB-231 and MDA-MB-468 are characterized by high expression of stem cell markers compared to the cells of low-grade malignancy (LGM), T47D and MCF-7: CD44 levels in T47D and MCF-7 cells were in range of 72-79 points, which is significantly lower than in HGM cells (p 0.05). Also, HGM cells with the properties of CSC were characterized by high expression of antiapoptotic proteins, the transcription factor Slug, and low levels of proapoptotic protein Bax (p 0.05) compared to LGM cells. In cells with CSC characteristics an increased expression of transferrin and its receptor, ferritin, fentorin and hepcidin was revealed indicating activation of the endogenous iron metabolism. The characteristic feature of HGM cells with CSC phenotype were the increased levels of oncogenic miR-221, -155 and -10b by 60%, 92% and 78%, respectively, and decreased levels of oncosuppressive miR-29b, -34a and -200b by 8.4 ± 0.3, 4.6 ± 0.2, and 3.4 ± 0.6 times compared to MCF-7 line cells. It has been established that the development of resistance to cytostatics is accompanied by increased aggressiveness of tumor cells, loss of expression of hormonal receptors and acquiring of stem phenotype. In particular, increased expression of P-gp was observed in BC cells during the development of resistance to doxorubicin, of GST during the development of resistance to cisplatin along with increased CD44 expression (p 0.05). We have revealed the relation between the presence of cells with the CSC phenotype (CD44+CD24-/low) and clinical and pathological characteristics of BC patients, their survival and BC sensitivity to neoadjuvant therapy (p > 0.05). Conclusions: The dependence between the expression of CSC markers and the degree of malignancy of tumor cells, development of resistance to cytostatics in vitro was established as well as the predictive value of the detection of the CSC for the individual prognosis of the BC course and sensitivity of the tumors to the treatment
Additional Access Points:
Chekhun, V. F.
Lukianova, N. Y.
Chekhun, S. V.
Bezdieniezhnykh, N. O.
Zadvomiy, T. V.
Borikun, T. V.
Polishchuk, L. Z.
Klyusov, O. M.

There are no free copies

Find similar

10.

Anti-cancer efficiency of natural killer cells differentiated from human adipose tissue-derived mesenchymal stem cells and transfected with miRNA150 [Text] / A. Karlitepe [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 212-218. - Bibliogr. at the end of the art.

MeSH-main:
СТРОМАЛЬНЫЕ КЛЕТКИ МЕЗЕНХИМНЫЕ -- MESENCHYMAL STROMAL CELLS (секреция, ультраструктура)
ИММУНОГИСТОХИМИЯ -- IMMUNOHISTOCHEMISTRY (использование, тенденции)
КЛЕТКИ-КИЛЛЕРЫ ЕСТЕСТВЕННЫЕ -- KILLER CELLS, NATURAL (ультраструктура)
ПОДЖЕЛУДОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- PANCREATIC NEOPLASMS (патофизиология, этиология)
ГЕНЫ СУПРЕССОРЫ ОПУХОЛЕВОГО РОСТА -- GENES, TUMOR SUPPRESSOR (физиология)
ИММУНОТЕРАПИЯ -- IMMUNOTHERAPY (тенденции)
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS
Annotation: The aim of this study is to investigate the effects of miR150 transfection on NK-like cells differentiated from adipose tissue derived mesenchymal stem cells (AD-MSCs). Methods: NK-like cells were differentiated from AD-MSCs and activated by miR150 transfection. Transfected/non-transfected NK-like cells were characterized by immunohistochemical and RTPCR analyzes. Apoptotic efficiency of the transfected/non-transfected NK-like cells on pancreatic cancer cells PANC1 were determined by TUNEL and RT-PCR. Results: In miR150-transfected cells, the increased expression of NK cell-specific genes such as GZMB, KIR2DL2, CD16, CD56, NKG2D, NKp46 and increased immunoreactivity of NK cell-specific surface marker CD314 (NKG2D) were evident. TUNEL assays showed that NK-like cells with/without transfection induced apoptosis in PANC1 cells in the same manner. The decrease in oncogene expression and the increase in the tumor suppressor gene expression in PANC1 cells upon co-culture with NK-like cells differentiated from AD-MSCs were more prominent following miRNA150 transfection. Conclusion: It was shown in vitro that NK-like cells could be obtained by differentiation from AD-MSCs and their efficiency could be increased via miR150 transfection. The results are encouraging for further clinical studies in improvement of immunotherapeutic approaches for cancer therapy
Additional Access Points:
Karlitepe, A.
Kabadayi, H.
Vatansever, S.
Gurdal, M.
Gunduz, C.
Ercan, G.

There are no free copies

Find similar

1-10 11-20 21-30 31-40 41-50 51-60

Standard

Advanced

Professional

Through the dictionary

UDC-navigator

Thematic navigator

Access statistics

© International Association of users and developers of electronic libraries and new information technology
(ELNIT Association)