Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Total number of found documents : 29
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1-20 21-29

Type of document : Magazine article
Edition cipher :
Author(s) : Lykhova O., Strokovska L., Kovaleva O., Bezdieniezhnych N., Semesiuk N., Adamenko I., Vorontsova A., Kudryavets Yu.
Title : Alternative direction of inhibition of malignant properties in tumor cells in vitro and in vivo by gene therapy with INF-beta gene in recombinant baculovirus vector
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 90 (Cipher ЕУ12/2017/39/1)
MeSH-main: НОВООБРАЗОВАНИЯ -- NEOPLASMS
ГЕННАЯ ТЕРАПИЯ -- GENETIC THERAPY
BACULOVIRIDAE -- BACULOVIRIDAE
Annotation: The aim of the study was to investigate the influence of recombinant baculovirus containing the interferon-β gene (rBV/IFN) on phenotypic characteristics of tumor cells in vitro: morphology, growth, cytogenetic characteristics and expression of proteins associated with proliferative activity, cell cycle regulation, epithelial-mesenchymal transition (EMT), invasiveness, and the migration potential
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Type of document : Magazine article
Edition cipher :
Author(s) : Sidorenko S.P., Romanets-Korbut O., Gordiienko I., Kovalevska L., Shlapatska L.
Title : CD150/SLAMF1 as a new potential target for anti-tumor therapy
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 90-91 (Cipher ЕУ12/2017/39/1)
MeSH-main: НОВООБРАЗОВАНИЯ -- NEOPLASMS
ПРОТИВООПУХОЛЕВЫЕ СРЕДСТВА -- ANTINEOPLASTIC AGENTS
Annotation: CD150/SLAMF1 is a prototype member of SLAM family within the immunoglobulin superfamily of surface receptors that are widely expressed on cells within hematopoietic system. Six of nine SLAMF receptors have a paired unique immunoreceptor tyrosine-based switch motif (ITSM) that serves as a docking site for SH2-containing proteins. In T and B lymphocytes, natural killer cells, macrophages and dendritic cells CD150 is a co-receptor molecule that mediates different signal transduction pathways depending on the availability of downstream signaling elements, especially, the adaptor protein SH2D1A/SAP. Due to highly glycosylated and sialylated extracellular Ig domains, CD150 is involved in homotypic interactions and could be considered as a pattern-recognizing receptor. It is a major entry receptor for several Morbilliviruses, including measles virus, and also a bacterial sensor that control the killing of Gram-negative bacteria. Functionally it serves as a bridge between innate and adaptive immunity
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Type of document : Magazine article
Edition cipher :
Author(s) : Gordiienko I., Shlapatska L., Kholodniuk V., Sklyarenko L., Sidorenko S.
Title : CD150/SLAMF1 antigen in molecular pathobiology of chronic lymphocytic leukemia
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 91-92 (Cipher ЕУ12/2017/39/1)
MeSH-main: ЛЕЙКОЗ ЛИМФОЦИТАРНЫЙ ХРОНИЧЕСКИЙ B-КЛЕТОЧНЫЙ -- LEUKEMIA, LYMPHOCYTIC, CHRONIC, B-CELL
АНТИГЕНЫ -- ANTIGENS
Annotation: Recent finding indicate that CD150 cell surface expression can be used as a surrogate prognostic marker of chronic lymphocytic leukemia (CLL) favorable outcome. It was shown that CLL patients with high CD150 expression level on malignant B cells has longer treatment free survival and overall survival compared to patients, which lost CD150 cell surface expression. However, the mechanisms that underlie dependent on CD150 expression biological properties of CLL B cells are not fully understood. Present study was focused on characterization of CD150 topology and isoforms expression, as well as study of CD150 mediated signaling pathways in CLL B cells
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Type of document : Magazine article
Edition cipher :
Author(s) : Bezdieniezhnych N., Lykhova O., Kocherga R., Kudryavets Yu.
Title : Biological characteristics of tumor cells at the different stages of EMT upon exposure to anticancer drugs and cytokines
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 89-90 (Cipher ЕУ12/2017/39/1)
MeSH-main: ПРОТИВООПУХОЛЕВЫЕ СРЕДСТВА -- ANTINEOPLASTIC AGENTS
ЦИТОКИНЫ -- CYTOKINES
ЭПИТЕЛИАЛЬНО-МЕЗЕНХИМНЫЙ ПЕРЕХОД -- EPITHELIAL-MESENCHYMAL TRANSITION
Annotation: Formation of a highly malignant metastatic tumor cell phenotype and resistance to anticancer drugs are associated with the implementation of epithelial- mesenchymal transition (EMT). Thus, monitoring of EMT and the possible inhibition of this process could be helpful to inhibit the tumor progression. It is quite often that anti-tumor treatment is not effective, so new modalities and new targets to combat cancer should be developed. Established cell lines, primary cultures of malignant cells obtained from biopsies or ascites of patients with epithelial cancers (breast, colorectal and ovarian), immunohistochemistry, and statistical methods were used
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Type of document : Magazine article
Edition cipher :
Title : Materials of international vactrain/ 3-rd Swedish-Ukrainian conference on cancer diseases, January 16-17, 2017, Stockholm, Sweden
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 88-93 (Cipher ЕУ12/2017/39/1)
MeSH-main: ОНКОЛОГИЯ МЕДИЦИНСКАЯ -- MEDICAL ONCOLOGY
НОВООБРАЗОВАНИЯ -- NEOPLASMS
СЪЕЗДЫ, СИМПОЗИУМЫ -- CONVENTIONS, SYMPOSIUMS
Annotation: The scientific conference ‘‘International VACTRAIN/ 3-rd Swedish-Ukrainian conference on cancer diseases’’ was held on January 16–17, 2017 at Karolinska Institutet, Stockholm, Sweden. These 1.5 days were filled with the lectures by the invited speakers and oral presentations selected on the basis of submitted abstracts from younger researchers. The meeting was focused on what we have learnt over the recent times, on the current situation, and on the important challenges for the future with focus on combating cancer
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Type of document : Magazine article
Edition cipher :
Author(s) : Ratushna O.O., Minchenko D.O., Riabovol O.O., Luzina O.Y., Minchenko O.H.
Title : Endoplasmic reticulum stress as a key factor of genome reprogramming in cancer cells
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 88-89 (Cipher ЕУ12/2017/39/1)
MeSH-main: НОВООБРАЗОВАНИЯ -- NEOPLASMS
ЭНДОПЛАЗМАТИЧЕСКОГО РЕТИКУЛУМА СТРЕСС -- ENDOPLASMIC RETICULUM STRESS
Annotation: The endoplasmic reticulum (ER) stress represents the unfolded protein response to cope with the accumulation of unfolded or misfolded proteins. It is required to maintain the functional integrity of the ER, which is a dynamic intracellular organelle with exquisite sensitivity to alterations in homeostasis. The unfolded protein response is a key player in the development of different malignant tumors. Depending on the duration and severity of the ER stress, it leads to cell adaptation or demise. This stress is a fundamental phenomenon, which provides a secure protection of the cells from different environmental challenges and is transduced by three major ER resident stress sensors. Activation of these ER stress sensors leads to transcriptional reprogramming of the cells. The signaling pathways elicited by those stress sensors have connections with metabolic pathways and with other plasma membrane receptor signaling networks. As such, the ER has an essential position as a signal integrator in the cell and is instrumental in the different phases of tumor progression
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Type of document : Magazine article
Edition cipher :
Author(s) : Horak I., Shytikov D., Geraschenko D., Knopfova L., Borsig L., Drobot L.
Title : The adaptor protein Ruk/CIN85 paradoxically enhances EMT of triple negative mouse breast adenocarcinoma 4T1 cells
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 89 (Cipher ЕУ12/2017/39/1)
MeSH-main: АДЕНОКАРЦИНОМА -- ADENOCARCINOMA
МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS
АДАПТЕРНЫЕ БЕЛКИ ВЕЗИКУЛЯРНОГО ТРАНСПОРТА -- ADAPTOR PROTEINS, VESICULAR TRANSPORT
ЭПИТЕЛИАЛЬНО-МЕЗЕНХИМНЫЙ ПЕРЕХОД -- EPITHELIAL-MESENCHYMAL TRANSITION
МЫШИ -- MICE
Annotation: To coordinate cellular responses, cell surface receptors employ receptor-associated adaptor proteins that are composed exclusively of domains and motives involved in intermolecular interactions. The assembling of adaptor proteins-mediated supramolecular complexes is regulated in dynamic and selective fashion, thereby influencing processing of information through signaling networks
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Type of document : Magazine article
Edition cipher :
Author(s) : Brieieva O., Nesina I., Buchynska L.
Title : Genotoxic sensitivity to 4-hydroxyestradiol in peripheral blood lymphocytes of endometrial cancer patients
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 93 (Cipher ЕУ12/2017/39/1)
MeSH-main: ЭНДОМЕТРИЯ НОВООБРАЗОВАНИЯ -- ENDOMETRIAL NEOPLASMS
ГИДРОКСИЭСТРОНЫ -- HYDROXYESTRONES
ЛИМФОЦИТЫ -- LYMPHOCYTES
Annotation: The endometrial cancer (EC) pathogenesis to a large extent may be determined by genotoxic effects of estrogen metabolites, among which the 4-hydroxyestradiol (4OHE2) is characterized by the most prominent DNA-damaging properties. Genomic instability and sensitivity to genotoxic estrogen metabolites in cells of EC patients depend on the DNA repair efficiency. The aim of the study was to analyze the level of DNA damage in peripheral blood lymphocytes (PBL) of EC patients after treatment with the 4OHE2 and to evaluate the repair efficiency of induced DNA damag
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Type of document : Magazine article
Edition cipher :
Title : Kyiv, May 11-12, 2017 Workshop with international participation. New trends in cancer research and innovative tumor vaccines
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 94 (Cipher ЕУ12/2017/39/1)
MeSH-main: НОВООБРАЗОВАНИЯ -- NEOPLASMS
ВАКЦИНЫ ПРОТИВООПУХОЛЕВЫЕ -- CANCER VACCINES
СЪЕЗДЫ, СИМПОЗИУМЫ -- CONVENTIONS, SYMPOSIUMS
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10.

Type of document : Magazine article
Edition cipher :
Title : International scientific conference "Normal and and cancer stem cells: discovery, diagnosis and therapy", October 5-6, 2017, Kyiv, Ukraine
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 95 (Cipher ЕУ12/2017/39/1)
MeSH-main: НОВООБРАЗОВАНИЙ СТВОЛОВЫЕ КЛЕТКИ -- NEOPLASTIC STEM CELLS
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11.

Type of document : Magazine article
Edition cipher :
Author(s) : Mankovska O., Kashuba V.
Title : Identification of novel molecular and genetic markers for early detection and prognosis of epithelial tumors
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 93 (Cipher ЕУ12/2017/39/1)
MeSH-main: НОВООБРАЗОВАНИЯ ЖЕЛЕЗИСТЫЕ И ЭПИТЕЛИАЛЬНЫЕ -- NEOPLASMS, GLANDULAR AND EPITHELIAL
ГЕНЕТИЧЕСКИЕ МАРКЕРЫ -- GENETIC MARKERS
Annotation: The main topic of our research is the identification of molecular and genetic markers for the early detection of epithelial tumors and prognosis of the course of disease. Early detection of carcinogenic process can lead to more successful treatment, thus, significantly increasing the chances for recovery. The investigation of non-invasive diagnostic tools, which can be used in clinical practice, is now the main focus of oncology. This problem can be solved by using of molecular nucleic acid markers that are present in biological fluids (serum, urine, semen etc)
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12.

Type of document : Magazine article
Edition cipher :
Author(s) : Borikun T., Lukianova N., Chekhun V.
Title : Changes in expression of miRNA-122, -200b, and -320a as prognostic biomarkers for breast cancer
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 92 (Cipher ЕУ12/2017/39/1)
MeSH-main: МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS
БИОМАРКЕРЫ ФАРМАКОЛОГИЧЕСКИЕ -- BIOMARKERS, PHARMACOLOGICAL
Annotation: High heterogeneity and diversity of breast tumors make the molecular characterization based on new biological markers very important. Recent studies have shown that not only genes and proteins are important players in tumorigenesis, but also a new class of potential epigenetic tumor markers such as microRNAs (miRNAs). An important advantage of miRNA molecules is their stability in both plasma and tumor tissues
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13.

Type of document : Magazine article
Edition cipher :
Author(s) : Mints M., Mushtag M., Iurchenko N., Kovalevska L., Buchynska L., Kashuba E.
Title : The MRPS 18-2 protein as a putative marker of carcinogenesis
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 92 (Cipher ЕУ12/2017/39/1)
MeSH-main: ЭНДОМЕТРИЯ НОВООБРАЗОВАНИЯ -- ENDOMETRIAL NEOPLASMS
НОВООБРАЗОВАНИЙ МАРКЕРЫ БИОЛОГИЧЕСКИЕ -- TUMOR MARKERS, BIOLOGICAL
БЕЛКИ НОВООБРАЗОВАНИЙ -- NEOPLASM PROTEINS
Annotation: Endometrial cancer (EC) is one of the most frequent causes of cancer death among women in developed countries. Histopathological diagnosis and imaging techniques for EC are limited, thus new prognostic markers are needed to offer patients the best treatment and follow-up
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14.

Type of document : Magazine article
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Author(s) : Mushtag M., Kovalevska L., Klein G., Kashuba E.
Title : High expression levels of MRPS 18-2 and presence of the RB protein are required for the maintenance of stem cell phenotype
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 92-93 (Cipher ЕУ12/2017/39/1)
MeSH-main: СТВОЛОВЫЕ КЛЕТКИ -- STEM CELLS
БЕЛКИ -- PROTEINS
Annotation: We have found that mitochondrial ribosomal protein MRPS18-2 (S18-2) is involved in regulation of the RB-dependent pathway. It binds to both hypo- and hyperphosphorylated RB. The binding between RB and S18-2 is promoted when cytoplasmic S18-2 is targeted to the nucleus, and this disrupts the association of E2F1 with RB, as indicated by the increased level of free E2F1 in the nucleus. This presumably enhances the RB-dependent block to S-phase entry in the cell cycle. We have also found overexpression of the human S18-2 in immortalized primary rat embryonic fibroblasts (REFs) that showed properties of embryonic stem cells. Elevated expression of S18-2 in stem cells (our findings and analysis of published microarray data) raises the question of whether this protein co-operates with the RB protein in differentiation and carcinogenesis
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15.

Type of document : Magazine article
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Author(s) : Savarese I., Grazzini M., Gori A., D’Errico A., Doni L., Scarfi F., Covarelli P., Di Costanzo F., De Giorgi V.
Title : Trichilemmal cystis in metastatic melanoma : A case report
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 86-87: цв. ил. (Cipher ЕУ12/2017/39/1)
Notes : 8 ref.
MeSH-main: МЕЛАНОМА -- MELANOMA
НОВООБРАЗОВАНИЙ МЕТАСТАЗЫ -- NEOPLASM METASTASIS
ПАРАНЕОПЛАСТИЧЕСКИЕ СИНДРОМЫ -- PARANEOPLASTIC SYNDROMES
КИСТЫ -- CYSTS
Annotation: The malignant melanoma is a neoplasm associated with a wide variety of cutaneous paraneoplastic syndromes, as dermatomyositis, systemic sclerosis, paraneoplastic pemphigus. We describe a case of four multiple trichilemmal cystis arising on frontal region in the same patient with brain metastasis and unknown primary melanoma and discuss their relationship
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16.

Type of document : Magazine article
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Author(s) : Mohammadi S., Zahedpanah M., Nikbakht M., Shaiegan M., Hamidollah Ghaffari S., Nikugoftar M., Rahmani B., Asl D.
Title : Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 30-35: il. (Cipher ЕУ12/2017/39/1)
Notes : 55 ref.
MeSH-main: ОСТЕОПОНТИН -- OSTEOPONTIN
ТРАНСКРИПЦИИ ФАКТОР RELB -- TRANSCRIPTION FACTOR RELB
БЕТА-КАТЕНИН -- BETA CATENIN
Annotation: In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway or β-catenin have been considered. Aim: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN and β-catenin genes. Materials and Methods: The U937 cells were treated with PTL at concentrations of 4 μM (IC25) or 6 μM (IC50) and with OPN siRNA for MTT assay and colony forming assay. Western blot analysis using antibodies against OPN was performed with lysates of PTL-treated cells. Quantitative real-time polymerase chain reaction was performed using primers for OPN siRNA, RelB, AKT1, mTOR, PTEN and β-catenin. Results: PTL reduces OPN protein level and down-regulates RelB mRNA in U937 cell line. Suppression of OPN with siRNA increases the cytotoxic effects of PTL. Also, mRNA expression of AKT1, mTOR, PTEN, and β-catenin decreases with PTL or OPN siRNA. Conclusion: Sensitivity of U937 cells to PTL can be associated with the reduction in expression of prosurvival mediators
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17.

Type of document : Magazine article
Edition cipher :
Author(s) : Kit Yu., Magorivska I., Bilyi R., Myronovskij S., Stoika R.
Title : Anti-histone H1 IgGs prossess proliferative activity towards human T-leukemia CEM cells
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 36-41: il. (Cipher ЕУ12/2017/39/1)
Notes : 34 ref.
MeSH-main: ЛЕЙКОЗ T-КЛЕТОЧНЫЙ -- LEUKEMIA, T-CELL
ВОЛЧАНКА КРАСНАЯ СИСТЕМНАЯ -- LUPUS ERYTHEMATOSUS, SYSTEMIC
КРОВИ СЫВОРОТКА -- SERUM
КЛЕТКИ ПРОЛИФЕРАЦИЯ -- CELL PROLIFERATION
ПЕРЕКРЕСТНЫЕ РЕАКЦИИ -- CROSS REACTIONS
АУТОАНТИТЕЛА -- AUTOANTIBODIES
Annotation: The aim of this study was to characterize the proliferative activity of the anti-histone H1 IgGs towards human T-leukaemia CEM cells. Materials and Methods: Anti-histone H1 IgGs were purified from blood serum of systemic lupus erythematosus patients by precipitation of serum proteins with 50% ammonium sulfate followed by a sequential affinity chromatography on Protein GSepharose and histone H1-Sepharose columns. To avoid contamination with other proteins, anti-histone H1 IgGs were subjected to strongly acidic pH 2.0 during gel filtration through HPLC column. The effects of the anti-histone H1 IgGs on cell viability and cell cycle were tested by MTS-assay and flow cytometry, correspondingly. The cross-reactivity of the anti-histone H1 antibodies towards heterogenetic and cellular antigens was evaluated by Western-blot analysis. Results: It was found that incubation of CEM cells with the HPLC-purified anti-histone H1 IgGs resulted in significant stimulation of cell growth by 46% after 48 h of incubation. These IgGs possess an antigenic poly-specificity to positively charged heterogenetic antigens and different cellular antigens. FITC-labeled and biotinylated anti-histone H1 IgGs are internalized by CEM cells and preferentially accumulated in the cytoplasm. Conclusion: The anti-histone H1 IgGs are shown to internalize human T-leukemia CEM and stimulate their proliferation. These IgGs are polyspecific toward cellular antigens
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18.

Type of document : Magazine article
Edition cipher :
Author(s) : Symchych T.V., Fedosova N.I., Karaman O.M., Yevstratieva L.M., Voyeykova I.M., Potebnia H.P.
Title : Anticancer effect and immunologic response to xenogeneic embryonic proteins in mice bearing Ehrlich solid carcinoma
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 42-48: табл. (Cipher ЕУ12/2017/39/1)
Notes : 27 ref.
MeSH-main: КАРЦИНОМА, ЭРЛИХА ОПУХОЛЬ -- CARCINOMA, EHRLICH TUMOR
ВАКЦИНЫ ПРОТИВООПУХОЛЕВЫЕ -- CANCER VACCINES
Annotation: To investigate anticancer and immunologic effects of chicken embryonic proteins (CEP) in mice bearing Ehrlich solid carcinoma. Materials and Methods: The study was carried out on male Balb/c mice bearing Ehrlich solid carcinoma. The immunizations were performed after the tumor transplantation. The immune status was assessed on days 7, 14, 21 and 28 after the tumor challenge. Cytotoxic activity (CAT) of macrophages (Mph), natural killer cells (NK), cytotoxic T-lymphocytes (CTL) and blood serum, as well as the influence of the blood serum on immune cells activity was checked in MTT-assay; Mph’s cytochemical activity was tested in NBT-assay; Ehrlich antigen-specific or CEP-specific antibodies were detected in ELISA-assay; medium size circulating immune complexes (CIC) were detected in reaction of 4.5% polyethylene glycol precipitation. Results: The immunization resulted in tumor growth suppression and significant 25.64% prolongation of the survival time. In both control and immunized mice with transplanted tumors antibodies specific to Ehrlich carcinoma antigens and to CEP were detected, but antibody response was more balanced in the treatment group. In the treatment group both cytochemical and CAT of Mph was moderately activated and well preserved until late stages of tumor development; CAT of NK and CTL remained in the range of the intact mice until day 28 after the tumor transplantation. The immunized mice were well protected from accumulation of CIC and suppressive activity of autologous blood serum. Conclusion: Collectively, our data indicate that CEP can elicit immunomodulating and immunoprotecting effects sufficient to provide tumor growth inhibition. The further elaboration of a xenogeneic anticancer vaccine based on CEP is warranted
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19.

Type of document : Magazine article
Edition cipher :
Author(s) : Chekhun V.F., Lukianova N.Y., Borikun T.V., Zadvornyi T.V., Mokhir A.
Title : Artemisinin modulating effect on human breast cancer cell lines with different sensitivity to cytostatics
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 25-29: табл. (Cipher ЕУ12/2017/39/1)
Notes : 21 ref.
MeSH-main: МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS
АРТЕМИЗИНИНЫ -- ARTEMISININS
ЛЕКАРСТВЕННАЯ УСТОЙЧИВОСТЬ -- DRUG RESISTANCE
Annotation: To explore effects of Artemisinin on a series of breast cancer cells with different sensitivity to typical cytotoxic drugs (doxorubicin — Dox; cisplatin — DDP) and to investigate possible artemisinin-induced modification of the mechanisms of drug resistance. Materials and Methods: The study was performed on wild-type breast cancer MCF-7 cell line (MCF-7/S) and its two sublines MCF-7/Dox and MCF-7/DDP resistant to Dox and DDP, respectively. The cells were treated with artemisinin and iron-containing magnetic fluid. The latter was added to modulate iron levels in the cells and explore its role in artemisinin-induced effects. The MTT assay was used to monitor cell viability, whereas changes of expression of selected proteins participating in regulation of cellular iron homeostasis were estimated using immunocytochemical methods. Finally, relative expression levels of miRNA-200b, -320a, and -34a were examined by using qRT-PCR. Results: Artemisinin affects mechanisms of the resistance of breast cancer cells towards both Dox and DDP at sub-toxic doses. The former drug induces changes of expression of iron-regulating proteins via different mechanisms, including epigenetic regulation. Particularly, the disturbances in ferritin heavy chain 1, lactoferrin, hepcidin (decrease) and ferroportin (increase) expression (р ≤ 0.05) were established. The most enhanced increase of miRNA expression under artemisinin influence were found for miRNA-200b in MCF-7/DDP cells (7.1 ± 0.98 fold change), miRNA-320a in MCF-7/Dox cells (2.9 ± 0.45 fold change) and miRNA-34a (1.7 ± 0.15 fold change) in MCF-7/S cells. It was observed that the sensitivity to artemisinin can be influenced by changing iron levels in cells. Conclusions: Artemisinin can modify iron metabolism of breast cancer cells by its cytotoxic effect, but also by inducing changes in expression of iron-regulating proteins and microRNAs (miRNAs), involved in their regulation. This modification affects the mechanisms that are implicated in drug-resistance, that makes artemisinin a perspective modulator of cell sensitivity towards chemotherapeutic agents in cancer treatment
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20.

Type of document : Magazine article
Edition cipher :
Author(s) : Cherdyntseva N.V., Litviakov N.V., Denisov E.V., Gervas P.A., Cherdyntsev E.S.
Title : Circulating tumor cells in breast cancer : functional heterogeneity, pathogenetic and clinical aspects
Place of publication : Experimental Oncology. - К., 2017. - Том 39, N 1. - С. 2-11: il. (Cipher ЕУ12/2017/39/1)
Notes : 84 ref.
MeSH-main: МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS
ЛЕКАРСТВЕННАЯ ТЕРАПИЯ -- DRUG THERAPY
НОВООБРАЗОВАНИЙ ЦИРКУЛИРУЮЩИЕ КЛЕТКИ -- NEOPLASTIC CELLS, CIRCULATING
БИОПСИЯ -- BIOPSY
Annotation: Each patient has a unique history of cancer ecosystem development, resulting in intratumor heterogeneity. In order to effectively kill the tumor cells by chemotherapy, dynamic monitoring of driver molecular alterations is necessary to detect the markers for acquired drug resistance and find the new therapeutic targets. To perform the therapeutic monitoring, frequent tumor biopsy is needed, but it is not always possible due to small tumor size or its regression during the therapy or tumor inaccessibility in advanced cancer patients. Liquid biopsy appears to be a promising approach to overcome this problem, providing the testing of circulating tumor cells (CTC) and/or tumor-specific circulating nucleic acids. Their genomic characteristics make it possible to assess the clonal dynamics of tumors, comparing it with the clinical course and identification of driver mutation that confer resistance to therapy. The main attention in this review is paid to CTC. The biological behavior of the tumor is determined by specific cancerpromoting molecular and genetic alterations of tumor cells, and by the peculiarities of their interactions with the microenvironment that can result in the presence of wide spectrum of circulating tumor clones with various properties and potentialities to contribute to tumor progression and response to chemotherapy and prognostic value. Indeed, data on prognostic or predictive value of CTC are rather contradictory, because there is still no standard method of CTC identification, represented by different populations manifesting various biological behavior as well as different potency to metastasis. Circulating clasters of CTC appear to have essentially greater ability to metastasize in comparison with single CTC, as well as strong association with worse prognosis and chemoresistance in breast cancer patients. The Food and Drug Administration (USA) has approved the CTC-based prognostic test for clinical application in patients with advanced breast cancer. Prospective clinical trials have demonstrated that measuring changes in CTC numbers during treatment is useful for monitoring therapy response in breast cancer patients. Molecular and genetic analysis of CTC gives the opportunity to have timely information on emergence of resistant tumor clones and may shed light on the new targets for pathogenetic antitumor therapy
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