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Total number of found documents : 16
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1.


    Karaman, O. M.
    Macrophages - a perspective target for antineoplastic immunotherapy / O. M. Karaman, A. V. Ivanchenko, V. F. Chekhun // Experimental Oncology. - 2019. - Том 41, N 4. - P282-290

Annotation: The review discusses the data on the functional/phenotypic M1/M2 types of macrophages and their chimeric forms, the molecular mechanisms of polarization of these cells, and their role in the development of malignant tumors. Information on the prognostic value of the presence (density and location) of M1/M2 cells in tumor tissue is analyzed. Our own results evidence on the necessity of determination of the functional/phenotypic state of M1/M2 macrophages from different biological niches in the dynamics of tumor growth, in particular in terms of NO level and arginase activity
Additional Access Points:
Ivanchenko, A. V.
Chekhun, V. F.

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2.


   
    Nоn-linear dynamics theory and malignant melanoma / V. E. Orel [et al.] // Experimental Oncology. - 2019. - Том 41, N 4. - P291-299

Annotation: Chaos theory (nonlinear dynamics) defines cancer as a complex adaptive system in which each cyclic point corresponds to the bifurcation at which changes in signaling pathways emerge. Quantitative assessment of chaos in digital medical images such as electron microscopy, histology and cytology sections collected from patients with malignant cutaneous melanoma employed the following calculation parameters: the irregularity of external contours, internal heterogeneity based on brightness distribution of macromo­lecules, chromosomes, organelles, inclusion bodies, cells and tissues, kurtosis, entropy and the asymmetry coefficient. The present study undertook a nonlinear analysis of the chaotic hierarchy of malignant melanoma. However, considerably more studies will need to be carried out to determine the exact interrelationship between different levels of the hierarchy in the biological system. Multidisciplinary collaborations are therefore essential to find evidence to answer questions that remain open to researchers and oncologists
Additional Access Points:
Orel, V. E.
Korovin, S. I.
Molnair, J.
Orel, V. B.

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3.


   
    Cytomegaloviruses and malignant brain tumors / N. I. Lisyany [et al.] // Experimental Oncology. - 2019. - Том 41, N 4. - P300-303

Annotation: The review analyzes in detail the data on cytomegalovirus (CMV) as the cause of the development of malignant brain tumors. The use of modern methods of immunohistochemistry and polymerase chain reaction makes it possible to detect both individual proteins and CMV genes in tumor tissue, while virus cannot be isolated from tumor tissue using classical virological methods. The paper discusses the theories of “hit-and-run” and “microinfections”, which explain the mechanism of action of CMV. The data on various molecular mechanisms of transformation of normal cells into tumor cells under the action of CMV are presented. The presence of CMV was shown not only in tumor cells, but also in neural stem cells, monocytes and macrophages. The possibility of using immunotherapy with T-lymphocytes and CMV-based dendritic cellular vaccines for the treatment of cancer patients is discussed in detail. Clinical data on their effectiveness are presented. Three possible mechanisms of the action of immunotherapeutic drugs containing CMV antigens are considered
Additional Access Points:
Lisyany, N. I.
Klyuchnikova, A. A.
Belskaya, L. N.
Lisyany, A. A.
Gnedkova, I. A.

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4.


   
    Proteomics of transforming growth factor β1 (TGF β1) signaling in 184A1 human breast epithelial cells suggests the involvement of casein kinase 2α in TGF β1-dependent p53 phosphorylation at Ser392 / H. Woksepp [et al.] // Experimental Oncology. - 2019. - Том 41, N 4. - P304-311

Annotation: Transforming growth factor β1 (TGF β1) is a potent regulator of breast tumorigenesis. It inhibits proliferation of carcinoma cells, but the strength of its inhibitory action varies for cells from benigh, non-metastatic or metastatic tumors. The aim of this work was to generate a proteome profile of TGF β1 action on non-tumorigenic human breast epithelial cells 184A1, and validate predicted involvement of casein kinase 2α (CK2α), p53 and structure-specific recognition protein-1 (SSRP1). Materials and Methods: Two-dimensional gel electrophoresis and mass spectrometry were used to identify TGF β1-regulated proteins in 184A1 human breast immortalized non-tumorigenic cells. 184A1 cells may serve as a model of benign breast neoplasia. These cells were obtained from normal mammary tissue, were immortalized but are not malignant, and were obtained from the American Type Culture Collection. The systemic analysis was performed by using the Cytoscape tool. Transfection of cells with CK2α construct and small interfering RNAs to CK2α and SSRP1 were used to assess an impact of CK2α and SSRP1 on phosphorylation of the p53 and cell proliferation. Results: Proliferation of 184A1 cells was transiently inhibited by TGF β1. We identified 100 and 47 unique proteins which changed their expression and/or 35S-incorporation, respectively, upon treatment with TGF β1 for 2 h, 8 h or 24 h. Cell proliferation, death, migration, and metabolism were among the biological regulatory processes retrieved by the network analysis as affected by the identified proteins. The network analysis suggested that TGF β1 may affect the phosphorylation of p53 at Ser392 by engaging CK2α. This was confirmed by the immunoblotting and cell proliferation assays. Conclusion: We report here the list of 147 TGF β1-regulated proteins in immortalized non-tumorigenic human breast epithelial cells, and show involvement of CK2α in the regulation of p53 Ser392 phosphorylation
Additional Access Points:
Woksepp, H.
Saini, R. K. R.
Zakharchenko, O.
Gautier, A.
Souchelnytskyi, N.
Souchelnytskyi, S.

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5.


   
    Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB 4 leukemia cells via down-regulation of BIRC 5 expression / Nayeri M. J. Dehghan [et al.] // Experimental Oncology. - 2019. - Том 41, N 4. - P312-317

Annotation: It has been reported that spiroaminopyrimidine derivatives inhibited the growth and proliferation of various cancer cell lines. In the present study, we evaluated cytotoxic and apoptosis-inducing effects of 2,4-diamino-1,3-diazaspiro[5.5]-9-tert-butyl-2, 4-diene-5-carbonitril (9-tBAP) on NB4 acute promyelocytic leukemia (APL) cells. Materials and Methods: The cells were treated with 10–100 µM of 9-tBAP. Cytotoxic activity of the compound was measured using the MTT assay. Apoptosis was investigated by Hoechst 33258 staining as well as by Annexin V/PI double staining. Results: The compound under study was found to be highly active cell growth inhibitor with IC50 of 30.0 ± 3.5 µM inducing apoptosis in NB4 cells. Cell cycle analysis by flow cytometry showed a time-dependent increase in sub-G1 cell population. Real-time polymerase chain reaction analysis revealed that the treatment with the compound down-regulated the BIRC5 expression in a time-dependent manner. Conclusion: 9-tBAP displayed potent anti-leukemic activity in vitro thus warranting further investigation
Additional Access Points:
Dehghan, Nayeri M. J.
Mahdavi, M.
Fazeli, H.
Hosseinpour, Feizi M. A.

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6.


   
    Fut3 expression in human breast cancer cells under hypoxia and serum deprivation / A. P. B. Albuquerque [et al.] // Experimental Oncology. - 2019. - Том 41, N 4. - P318-322

Annotation: This study aimed to investigate the effects of hypoxia and serum deprivation on regulation of fucosyltransferase-3 (FUT3) expression in breast cancer cells. Materials and Methods: FUT3 expression was evaluated in T47D and MCF7 cells. Transcriptional and protein analysis was performed under hypoxia and serum deprivation conditions after 6 and 24 hours; and after 24 and 48 hours, respectively. Results: In T47D cells, experimental conditions induced a significant decrease in FUT3 expression at both, transcriptional and protein levels, while in MCF7 cells the same conditions induced a significant increase of FUT3 expression. Conclusions: Regulation of FUT3 expression under hypoxic and serum deprivation conditions may be involved in the acquisition of advantages related to apoptosis resistance and metastasis promotion, according to the intrinsic differences presented by T47D and MCF7 cells
Additional Access Points:
Albuquerque, A. P. B.
Silva, A. L. V.
Lima, C. A. D.
Beltrao, E. I. C.

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7.


   
    Deletions in metastatic colorectal cancer with chromothripsis / E. Skuja [et al.] // Experimental Oncology. - 2019. - Том 41, N 4. - P323-327

Annotation: In our previously reported study, we found a correlation between DNA massive fragmentation and increased progression free survival (PFS) in metastatic colorectal cancer (mCRC), but not overall survival. The aim of this study is to find overlapping deleted genome regions in selected mCRC patients with chromothripsis and detect possible cause of increased PFS, and find new genes or combinations, involved in colorectal cancer oncogenesis. Materials and Methods: 10 mCRC patients with chromothripsis receiving 5-fluorouracil, oxaliplatin, leucovorin (FOLFOX) first-line palliative chemotherapy between August, 2011 and October, 2012 were selected for this study. Microarray analysis was performed using the Infinium HumanOmniExpress-12 v1.0 formalin-fixed paraffin-embedded (FFPE) BeadChip kit (Illumina). BeadChip was scaned on HiScan (Illumina). Analysis was performed by GenomeStudio software (Illumina) and R version 3.1.2. Copy number variation and breakpoints on the chromosomes were analyzed using the DNA copy package. Results: Eight deleted tumor suppressor genes (ROBO2, CADM2, FAT4, PCDH10, PCDH18, CDH18, TSG1, CTNNA3) and four deleted oncogenes (CDH12, GPM6A, ADAM29, COL11A1) were identified in more than half of patients. In 70% patients’ deletion in COL11A1 was detected. Deletion of MIR1269, MIR4465, MIR1261 and MIR4490 in patients with longer time to progression was observed. Four patients (40%) with PFS over 14 months, presented with NRG3 deletion (oncogene, еpidermal growth factor receptor (EGFR) ligand) what could possibly decrease proliferation of cancer cells via decreasing EGFR activation. Conclusions: Multiple chromosomal deletions (MIR1269, NRG3, ADK) in mCRC patients with chromothripsis are associated with better response to first line palliative FOLFOX-type chemotherapy and increased PFS
Additional Access Points:
Skuja, E.
Butane, D.
Nakazawa-Miklasevica, M.
Daneberga, Z.
Purkalne, G.
Miklasevics, E.

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8.


   
    Molecular mechanisms of oxidation damage and liver cell dysfunction in patients with metastatic colorectal cancer / A. P. Burlaka [et al.] // Experimental Oncology. - 2019. - Том 41, N 4. - P328-334

Annotation: Interaction between tumor cells and tumor microenvironment is critical for homeostasis of normal cells and tumor growth. Tumor cell — stroma interaction represents the potent factor able to initiate cancer and affect tumor progression and disease outcome. The tumors vary by their origin and microenvironment (proportion of stromal cells, their composition and activation state). The surgical stress and tumor microenvironment may potentiate acute hepatic failure in the patients with metastatic colorectal cancer (mCRC). Pathological effect of ischemia-reperfusion (I/R) consists in the increased generation of superoxide radicals (SR) and nitrogen oxide (NO) affecting the postresectional regeneration of liver tissue. Redox state of hepatic tissue in I/R setting upon resection of metastases may trigger the aggressiveness of residual cancer cells and regeneration or degradation of hepatic tissue. The aim of the study was to analyze redox state of hepatic tissue following surgery with Pringle maneuver (PM) in the patients with mCRC. Materials and Methods: mCRC samples from 145 patients treated at National Cancer Institute, Ministry of Health of Ukraine, were analyzed. The patients obtained chemotherapy according to the approved international and national standards as well as clinical protocols. Two groups of patients were delineated according to the duration of the interruption of blood inflow due to PM, namely ≤ 45 min and 45 min. The activity of FeS proteins in the electron transport chain (ETC) in mitochondria and lactoferrin (LF) level in the tissues were assessed by EPR (77К). The rates of SR and NO generation were determined with spin traps. The activity of matrix metalloproteinase (MMP)-2 and -9 was measured by gelatin zymography using SDS-polyacrylamide gel electrophoresis. Results: In tissue of liver resected in the setting of 45 min ischemia, ETC function in mitochondria was impaired (decreased activity of FeS protein of N-2 ETC complex I due to interaction with NO). This results in the hypoxia state and glycolysis with uncontrolled SR generation. In addition, the efficiency of detoxification system in hepatocytes is reduced substantially with increase in semiquinone and LF levels as well as MMP-2 and -9 activity as compared with liver without metastatic lesions that was not affected by I/R. Conclusions: The ischemic injury of liver in the setting of metastasis resection results from cell response to interruption of blood flow followed by reperfusion. The key factor in the genesis of reperfusion damage is uncontrolled increase of the levels of SR and their metabolites — reactive oxygen species as well as the increased MMP activity. Also, liver tissue affected by I/R contains high levels of xanthine oxidase metabolizing hypoxanthine and monoamine oxidase deaminizing biogenic amines. Both processes are the sources of SR
Additional Access Points:
Burlaka, A. P.
Burlaka, A. A.
Virko, S. V.
Ganusevich, I. I.

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9.


    Buchynska, L. G.
    Morphological characteristics and expression of adhesion markers in cells of low differentiated endometrial carcinoma / L. G. Buchynska, L. A. Naleskina, I. P. Nesina // Experimental Oncology. - 2019. - Том 41, N 4. - P335-341

Annotation: The aim of the study was to evaluate the morphological features of endometrioid carcinoma of the endometrium (ECE) of low differentiation grade with different invasive potential and to characterize their molecular phenotype by the expression of a number of adhesion markers. Materials and Methods: We have studied the samples of operation material of 37 patients with ECE of low differentiation grade with deep invasion ( ½ myometrium), n = 26, and with invasion ½ myometrium, n
Additional Access Points:
Naleskina, L. A.
Nesina, I. P.

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10.


    Zelinskaya, A.
    Immunocytochemical characteristics of thyrocytes in radioiodine refractory metastases of papillary thyroid cancer / A. Zelinskaya // Experimental Oncology. - 2019. - Том 41, N 4. - P342-345

Annotation: The aim of the work was to carry out an immunocytochemical (ICC) study of thyroid peroxidase (TPO) and thyroglobulin (Tg) expression in the punctates of the radioiodine (RI) refractory and RI uptake metastases of thyroid papillary carcinoma (PTC), on the basis of which it is possible to develop new methods of preoperative prediction of RI resistance and RI therapy efficiency for thyroid papillary carcinoma metastases. Materials and Methods: The ICC research was carried out on a fine needle aspiration biopsy material of 104 metastases that were found after thyroidectomy and RI therapy, i.e. in postoperative period (79 — radioiodine-refractory metastases, 25 — radioiodine-uptake metastases). The ICC analysis of TPO and Tg expression in PTC was performed with the use of monoclonal antibodies against TPO and Tg. Results: RI-refractory (RIRM) and RI-uptake metastases of PTC significantly differ by the percentage of cells expressing TPO and Tg (p 0.05, and p 0.05 respectively). The effectiveness of RI therapy was different for patients with different percentages of TPO-positive cells. Conclusion: A statistically significant difference was demonstrated in the expression of TPO in a punctates of RI-resistant and RI-sensitive metastatic TPC, based on which a new method for preoperative prediction of RI resistance and RI therapy efficiency was developed. It was shown that ICC determination of Tg expression in metastases is effective in preoperative monitoring of RI resistance of PTC if the part of Tg-positive cells in punctates is lower than 56%. The comprehensive study of the ICC profile of thyrocytes in RI-uptake metastases punctates allows us to develop a personified approach to prediction, monitoring and therapy of patients with PTC
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11.


    Moazeni-Roodi, A.
    Association bewtween XPO5 rs 1 1077 polymorphism and cancer susceptibility: a meta-analysis of 7284 cases and 85 1 1 controls / A. Moazeni-Roodi, M. Taheri, M. Hashemi // Experimental Oncology. - 2019. - Том 41, N 4. - P346-352

Annotation: Several studies evaluated the association between rs11077 polymorphism located in the 3’UTR of the XPO5 gene and cancer susceptibility. We conducted a meta-analysis to assess the impact of XPO5 rs11077 polymorphism on cancer risk. Materials and Methods: The online databases were searched for relevant case-control studies published up to July 2018. 15 articles of 16 studies, with totally 7284 cancer cases and 8511 healthy controls, were eligible for inclusion in the meta-analysis. The data were extracted from the eligible studies and were processed using Stata 14.1 and Revman 5.3 software. Pooled estimates of odds ratio with 95% confidence intervals were used to evaluate the strength of association between XPO5 rs11077 and cancer risk. Results: Overall, our finding showed no significant association between XPO5 rs11077 variant and overall cancer risk, either performed subgroup analysis by cancer types and ethnic groups in all genetic model. Conclusion: The findings did not support an association between rs11077 variant and cancer risk. Due to small sample sizes particularly in stratified analysis, further large-scale well designed studies between this polymorphism and cancer risk are warranted
Additional Access Points:
Taheri, M.
Hashemi, M.

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12.


   
    How to improve quality of life in patients with acute leukemia and comorbid ischemic heart disease treated with anthracycline-based induction chemotherapy / I. Skrypnyk [et al.] // Experimental Oncology. - 2019. - Том 41, N 4. - P353-356

Annotation: To evaluate the quality of life (QoL) parameters in patients with acute leukemia (AL) during standard induction chemotherapy, depending on the presence of concomitant ischemic heart disease and to improve them by the prevention of anthracycline cardiotoxicity with L-arginine. Materials and Methods: A total of 147 adult AL patients (72 males and 75 females with the mean age 54.7 ± 9.3 years) were enrolled in the study. QoL assessment was performed at baseline and after induction chemotherapy using SF-36 questionnaire. Both physical and mental parameters were evaluated. Results: The QoL analysis of patients with AL at the time of initial diagnosis showed extremely low QoL level in all subgroups compared with healthy individuals. It should be noted that the level of patients’ QoL after achieving remission remained significantly lower than those of practically healthy, which is primarily due to the need for further long-term treatment and, probably, the fear of the disease relapse development. It was found that the administration of L-arginine during induction chemotherapy in order to reduce the risk of anthracycline cardiotoxicity development has allowed improving the QoL in patients with concomitant ischemic heart disease. Conclusion: L-arginine decreases the risk of anthracycline-induced myocardial injury and improves QoL in AL patients
Additional Access Points:
Skrypnyk, I.
Maslova, G.
Lymanets, T.
Gusachenko, I.

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13.


   
    Association of elevated vitamin B12 with oncohematological diseases in a cohort of 79, 524 patients from Lanvia / D. Gavars [et al.] // Experimental Oncology. - 2019. - Том 41, N 4. - P357-362

Annotation: Currently there are some large-scale studies of elevated total vitamin B12 in relation to diseases and their prognosis. Aim of this retrospective study was to determine association of increased B12 as an additional diagnostic marker of oncohematological diseases by a statistical analysis of clinical data of 79,524 patients. Materials and Methods: Overall Latvian population representative data on B12 testing in 79,524 patients were obtained from laboratory database. The following exclusion criteria were applied: fluctuating B12 results within a three-month period, elevated ( 100 U/L) alanine transaminase or aspartate transaminase, hepatitis (HAV, HBV, and HCV) infection, reduced glomerular filtration rate ( 45 mL/min/1.73 m2). As a control group, individuals with normal B12 level and any oncologic diagnosis (solid cancer or hematological malignancies) were selected. Results: After application of step-by-step exclusion filters, 1,373 patients were left with significantly increased level of plasma B12 ( 1,700 pg/mL). Odds ratios for oncohematological diseases in total and myeloid leukemia (including acute, chronic and unspecified) in patient group with elevated B12 were found to be 6.0 (95% CI 4.7–7.6; p 0.0001) and 19.2 (95% CI 13.1–28.0; p 0.0001), respectively, as compared to the control group. Conclusion: Elevated total B12 could be considered as a potential marker for oncohematological disorders
Additional Access Points:
Gavars, D.
Perminov, D.
Tauckels, E.
Lindenberga, I.
Auce, A.
Lejniece, S.

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14.


    Prylutskyi, M. P.
    Detection of polyamines using immunobiosensor based on zinc oxide nanoparticles / M. P. Prylutskyi, M. F. Starodub // Experimental Oncology. - 2019. - Том 41, N 4. - P363-365

Annotation: To analyze the performance of biosensor based on nanoparticles of zinc oxide for the detection of spermine and spermidine in solution and in cell culture. Materials and Methods: Zinc oxide nanoparticles were used for preparing biosensor containing antibodies to spermine and spermidine. Polyamine concentration is solutions of spermine and spermidine as well as in lyophilisate of MCF-7 cells was measured by luminescence of the samples excited by laser beam at 380 nm. Results: The minimum concentration for the detection of polyamines in model solutions is 10 ng/ml, and maximum one is 100 ng/ml. A higher level of luminescence intensity of nanoparticles was found during analysis the polyamines in MCF-7 lyophilisate allowing for detecting polyamines at concentrations from 100 cells/ml to 100,000 cells/ml. Conclusions: The proposed biosensor system for determining the level of biogenic polyamines in cell lyophilisate using the optical properties of zinc oxide nanoparticles is promising for further improvement of the methodology and its implementation for detection and measurement of polyamines in biological systems
Additional Access Points:
Starodub, M. F.

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15.


    Shkatula, P. M.
    International scientific conference "Tumor and Host: novel aspects of old problem" / P. M. Shkatula // Experimental Oncology. - 2019. - Том 41, N 4. - P366-367

Annotation: On November 21–22, 2019, the R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of National Academy of Sciences of Ukraine held the Second International Conference “Tumor and Host: Novel Aspects of Old Problem” in the Great Conference Hall of the National Academy of Sciences of Ukraine. The problem of the tumor-host relationship is a traditional priority for the Institute, the leading institution in the field of cancer research in Ukraine: the concept of the relationship between the tumor and the host has been put forward by the first director of the Institute, Academician R.E. Kavetsky, whose 120th anniversary is celebrated by the Ukrainian scientific community.
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16.


   
    To the 120th birthday anniversary of academician R. E. Kavetsky // Experimental Oncology. - 2019. - Том 41, N 4. - P368-369

Annotation: On December 1, 2019, the academic community commemorated the 120th birthday anniversary of Academician Rostislav Evge­nievich Kavetsky, the famous oncologist and pathophysiologist, the founder of the Ukrainian school of experimental oncology, the organizer and the first director of the Institute that now bears his name
Additional Access Points:
Kavetsky, Rostislav Evgenievich (physiologist ; 1899-1978) \о нем\

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