Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Total number of found documents : 14
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Type of document : Magazine article
Edition cipher :
Author(s) : Uspenska K. R., Gergalova G. L., Lykhmus O. Yu., Skok M. V.
Title : The effect of amixin and agmatine on cytochrome c release from isolated mitochondria
Parallel titles :Вплив аміксину та агматину на вивільнення цитохрому с з ізольованих мітохондрій
Place of publication : Український біохімічний журнал. - 2016. - Т. 88, № 1. - С. 5-10 (Cipher УУ1/2016/88/1)
Notes : Бібліогр.: в кінці ст.
MeSH-main: ТИЛОРОН -- TILORONE
РЕЦЕПТОРЫ НИКОТИНОВЫЕ -- RECEPTORS, NICOTINIC
ЦИТОХРОМОВ C ГРУППА -- CYTOCHROME C GROUP
МИТОХОНДРИИ -- MITOCHONDRIA
АГМАТИН -- AGMATINE
МОДЕЛИ НА ЖИВОТНЫХ -- MODELS, ANIMAL
Annotation: Mitochondrial nicotinic acetylcholine receptors (nAChRs) control permeability transition pore formation and cytochrome c release in the presence of apoptogenic factors. This study demonstrates that pharmacological agents amixin and agmatine affect mitochondrial nAChR functioning: they slightly suppress cytochrome c release from mouse brain and liver mitochondria stimulated with apoptogenic dose of Са2+ and prevent the effect of α7 nAChR agonist PNU282987. We conclude that mitochondria may be one of therapeutic targets of amixin and agmatine
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Type of document : Magazine article
Edition cipher :
Author(s) : Minchenko O. H., Tsymbal D. O., Minchenko D. O., Riabovol O. O., Ratushna O. O., Karbovskyi L. L.
Title : Hypoxic regulation of the expression of cell proliferation related genes in U87 glioma cells upon inhibition of IRE1 signaling enzyme
Parallel titles :Регуляція експресії генів, що мають відношення до проліферації клітин, за умов гіпоксії та пригнічення сигнального ензиму IRE1 у клітинах гліоми лінії U87
Place of publication : Український біохімічний журнал. - 2016. - Т. 88, № 1. - С. 11-21 (Cipher УУ1/2016/88/1)
Notes : Бібліогр.: в кінці ст.
MeSH-main: ГЕННОЙ ЭКСПРЕССИИ РЕГУЛЯЦИЯ -- GENE EXPRESSION REGULATION
ЭНДОПЛАЗМАТИЧЕСКОГО РЕТИКУЛУМА СТРЕСС -- ENDOPLASMIC RETICULUM STRESS
КЛЕТКИ ПРОЛИФЕРАЦИЯ -- CELL PROLIFERATION
ФЕРМЕНТЫ -- ENZYMES
КЛЕТОЧНАЯ ГИПОКСИЯ -- CELL HYPOXIA
ГЛИОМА -- GLIOMA
Annotation: We have studied the effect of inhibition of IRE1 (inositol requiring enzyme 1), which is a central mediator of endoplasmic reticulum stress and a controller of cell proliferation and tumor growth, on hypoxic regulation of the expression of different proliferation related genes in U87 glioma cells. It was shown that hypoxia leads to up-regulation of the expression of IL13RA2, CD24, ING1, ING2, ENDOG, and POLG genes and to down-regulation - of KRT18, TRAPPC3, TSFM, andMTIF2 genes at the mRNA level in control glioma cells. Changes for ING1 and CD24 genes were more significant. At the same time, inhibition of IRE1 modifies the effect of hypoxia on the expression of all studied genes. In particular, it increases sensitivity to hypoxia of the expression of IL13RA2, TRAPPC3, ENDOG, and PLOG genes and suppresses the effect of hypoxia on the expression of ING1 gene. Additionally, it eliminates hypoxic regulation of KRT18, CD24, ING2, TSFM, and MTIF2 genes expressions and introduces sensitivity to hypoxia of the expression of BET1 gene in glioma cells. The present study demonstrates that hypoxia, which often contributes to tumor growth, affects the expression of almost all studied genes. Additionally, inhibition of IRE1 can both enhance and suppress the hypoxic regulation of these gene expressions in a gene specific manner and thus possibly contributes to slower glioma growth, but several aspects of this regulation must be further clarified
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Type of document : Magazine article
Edition cipher :
Author(s) : Sandeep S., Mitra B., Priyanka A., Neeraj K., Aseem B., Neelam C.
Title : Multiple parametric approaches to assess acute radiation lung injury of rats
Parallel titles :Різні параметричні підходи з метою оцінювання гострого променевого ураження легенів у щурів
Place of publication : Український біохімічний журнал. - 2016. - Т. 88, № 1. - С. 22-30 (Cipher УУ1/2016/88/1)
Notes : Бібліогр.: в кінці ст.
MeSH-main: МОДЕЛИ НА ЖИВОТНЫХ -- MODELS, ANIMAL
ЛУЧЕВЫЕ ПОВРЕЖДЕНИЯ ЭКСПЕРИМЕНТАЛЬНЫЕ -- RADIATION INJURIES, EXPERIMENTAL
ЛЕГКИЕ -- LUNG
БРОНХОАЛЬВЕОЛЯРНЫЙ ЛАВАЖ -- BRONCHOALVEOLAR LAVAGE
БИОХИМИЧЕСКИЕ ПРОЦЕССЫ -- BIOCHEMICAL PROCESSES
Annotation: The effect ofwhole body gamma irradiation (WBI) in single fraction was studied, as well as its influence on the secretion of various biochemical markers and cellular component that could be used as acute radiation lung injury marker. Sprague dawley rats were treated with WBI (60Co) of radiation dose from 1 Gy to 5 Gy (dose rate - 0.95 Gy/min). Bronchoalveolar lavage fluid was retrieved from all animals in control and radiation treated groups up to 72 h post radiation. Bronchoalveolar lavage fluid (BALF) was analyzed for lactate dehydrogenase (LDH), acid phosphatase (AP), alkaline phosphatase (ALP), cell count and total protein. Intragroup and intergroup comparison of BALF parameters at different radiation doses showed significant difference. LDH was significantly increased as the dose increased from 1Gy to 5Gy (P = 0.00) after 2 h with effect size of difference (r 0.3). ALP was significantly altered after 3Gy and 4Gy (P 0.05). AP was significantly altered at 2Gy-5Gy (P 0.05). Total protein level changed significantly from 1Gy to 5Gy (P 0.00). Cellular content of BALF showed significant changes after radiation exposure. BALF parameters like LDH, AP, ALP, neutrophils, lymphocytes, total leukocyte count and total protein were sensitive to radiation exposure and their levels vary significantly up to 72 h after single whole body radiation exposure in Sprague dawley rats. It can be concluded that the biochemical indices in BALF have more wide application in evaluation of acute radiation induced lung injury
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Type of document : Magazine article
Edition cipher :
Author(s) : Danylovych H. V.
Title : Evaluation of functioning of mitochondrial electron transport chain with NADH and FAD autofluorescence
Parallel titles :Оцінка функціонування електронтранспортувального ланцюга мітохондрій міометрія за автофлуоресценцією NADH та FAD
Place of publication : Український біохімічний журнал. - 2016. - Т. 88, № 1. - С. 31-43 (Cipher УУ1/2016/88/1)
Notes : Бібліогр.: в кінці ст.
MeSH-main: МЕМБРАННОГО ТРАНСПОРТА БЕЛКИ МИТОХОНДРИАЛЬНЫЕ -- MITOCHONDRIAL MEMBRANE TRANSPORT PROTEINS
МОДЕЛИ НА ЖИВОТНЫХ -- MODELS, ANIMAL
МИОМЕТРИЙ -- MYOMETRIUM
НАД -- NAD
ФЛАВИНАДЕНИНДИНУКЛЕОТИД -- FLAVIN-ADENINE DINUCLEOTIDE
Annotation: We prove the feasibility of evaluation of mitochondrial electron transport chain function in isolated mitochondria of smooth muscle cells of rats from uterus using fluorescence of NADH and FAD coenzymes. We found the inversely directed changes in FAD and NADHfluorescence intensity under normal functioning of mitochondrial electron transport chain. The targeted effect of inhibitors of complex I, III and IV changed fluorescence of adenine nucleotides. Rotenone (5 μΜ) induced rapid increase in NADH fluorescence due to inhibition of complex I, without changing in dynamics of FAD fluorescence increase. Antimycin A, a complex III inhibitor, in concentration of 1 μg/ml caused sharp increase in NADH fluorescence and moderate increase in FAD fluorescence in comparison to control. NaN3 (5 mM), a complex IV inhibitor, and CCCP (10 μΜ), a protonophore, caused decrease in NADH and FAD fluorescence. Moreover, all the inhibitors caused mitochondria swelling. NO donors, e.g. 0.1 mM sodium nitroprusside and sodium nitrite similarly to the effects of sodium azide. Energy-dependent Ca2+ accumulation in mitochondrial matrix (in presence of oxidation substrates and Mg-ATP2- complex) is associated with pronounced drop in NADH and FAD fluorescence followed by increasedfluorescence of adenine nucleotides, which may be primarily due to Ca2+- dependent activation of dehydrogenases of citric acid cycle. Therefore, the fluorescent signal of FAD and NADH indicates changes in oxidation state of these nucleotides in isolated mitochondria, which may be used to assay the potential of effectors of electron transport chain
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Type of document : Magazine article
Edition cipher :
Author(s) : Franskevych D. V., Grynyuk I. I., Prylutska S. V., Matyshevska O. P.
Title : Modulation of cisplatin-induced reactive oxygen species production by fullerene C(60) in normal and transformed lymphoid cells
Parallel titles :Модуляція індукованого цисплатином продукування активних форм кисню фулереном С(60) у нормальних та трансформованих лімфоїдних клітинах
Place of publication : Український біохімічний журнал. - 2016. - Т. 88, № 1. - С. 44-50 (Cipher УУ1/2016/88/1)
Notes : Бібліогр.: в кінці ст.
MeSH-main: ЛЕЙКОЗ ЛИМФОИДНЫЙ -- LEUKEMIA, LYMPHOID
БОЛЕЗНЬ, МОДЕЛИ НА ЖИВОТНЫХ -- DISEASE MODELS, ANIMAL
ЦИСПЛАТИН -- CISPLATIN
ФУЛЛЕРЕНЫ -- FULLERENES
КИСЛОРОДА АКТИВНЫЕ ФОРМЫ -- REACTIVE OXYGEN SPECIES
Annotation: The early response of normal (Wistar rat thymocytes) and transformed (mice lymphoid leukemia L1210) cells to treatment with anticancer drug cisplatin or to combined treatment with cisplatin and carbon nanostructure fullerene C60 was studied. We demonstrated with fluorescent probes DCFH-DA and TMRE that cisplatin at concentration 1 μg/ml induced reactive oxygen species (ROS) production and decreased the value of mitochondrial membrane potential in both cell types. The combined treatment with cisplatin (1 μg/ml) and fullerene C60 (7.2 μg/ml) was shown to be followed by oppositely directed modulation of ROS production in thymocytes and L1210 cells. Cisplatin-induced ROS production was intensified in L1210 cells, while in thymocytes it was decreased. It is supposed that the different effects of combined treatment are associated with peculiarities of fullerene C60 accumulation and localization in normal and cancer cells
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Type of document : Magazine article
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Author(s) : Kоbylinska L. I., Havrylyuk D. Ya., Mitina N. E., Zаichenko A. S., Lesyk R. B., Zіmenkovsky B. S., Stoika R. S.
Title : Biochemical indicators of nephrotoxicity in blood serum of rats treated with novel 4-thiazolidinone derivatives or their complexes with polyethylene glycol-containing nanoscale polymeric carrier
Parallel titles :Біохімічні показники нефротоксичності у сироватці крові щурів за дії нових похідних 4-тіазолідинонів та їхніх комплексів із поліетиленглікольвмісним полімерним нанорозмірним носієм
Place of publication : Український біохімічний журнал. - 2016. - Т. 88, № 1. - С. 51-60 (Cipher УУ1/2016/88/1)
Notes : Бібліогр.: в кінці ст.
MeSH-main: МОДЕЛИ НА ЖИВОТНЫХ -- MODELS, ANIMAL
КРОВИ СЫВОРОТКА -- SERUM
ПОЧЕЧНАЯ ЭЛИМИНАЦИЯ -- RENAL ELIMINATION
ТИАЗОЛИДИНЫ -- THIAZOLIDINES
ДОКСОРУБИЦИН -- DOXORUBICIN
ЛЕКАРСТВ ОЦЕНКА ДОКЛИНИЧЕСКАЯ -- DRUG EVALUATION, PRECLINICAL
ПОЛИЭТИЛЕНГЛИКОЛИ -- POLYETHYLENE GLYCOLS
НАНОТЕХНОЛОГИЯ -- NANOTECHNOLOGY
Annotation: The aim of this study was to compare the effect of new synthetic 4-thiazolidinone derivatives (potential anticancer compounds denoted as 3882, 3288 and 3833) and doxorubicin (positive control) in free form and in their complexes with synthetic polyethylene glycol-containing nanoscale polymeric carrier on the biochemical indicators of nephrotoxicity in blood serum of rats. The concentration of total protein, urea, creatinine, glucose, ions ofsodium, potassium, calcium, iron and chloride was measured. It was found that after injection of the investigated compounds, the concentration of sodium cations and chloride anions in blood serum was increased compared with control (untreated animals). Doxorubicin’s injection was accompanied by a decrease in the concentration of iron cations. The concentration of total protein, urea and creatinine decreased under the influence of the studied compounds. Complexation of these antineoplastic substances with a synthetic polymeric nanocarrier lowered the concentration of the investigated metabolites substantially compared to the effect of these compounds in free form. The normalization of concentration of total protein, urea and creatinine in blood serum of rats treated with complexes of the studied compounds with the polymeric carrier comparing with increased concentration of these indicators at the introduction of such compounds in free form was found.
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Type of document : Magazine article
Edition cipher :
Author(s) : Rakhmetov A. D., Lee Sang Pil, Ostapchenko L. I., Chae
Title : Prx II and CKBB proteins interaction under physiological and thermal stress conditions in A549 and HeLa cells
Parallel titles :Взаємодія протеїнів Prx II та CKBB за умов теплового стресу в клітинах A549 та HeLa
Place of publication : Український біохімічний журнал. - 2016. - Т. 88, № 1. - С. 61-68 (Cipher УУ1/2016/88/1)
Notes : Бібліогр.: в кінці ст.
MeSH-main: ПЕРОКСИРЕДОКСИНЫ -- PEROXIREDOXINS
ТЕПЛОВОГО СТРЕССА СИНДРОМЫ -- HEAT STRESS DISORDERS
КРЕАТИНКИНАЗА -- CREATINE KINASE
ВЕСТЕРН-БЛОТТИНГ -- BLOTTING, WESTERN
Annotation: Peroxiredoxins (Prxs) are versatile enzymes that demonstrate various cell functions as peroxidases, protein chaperones, functions of signal modulators and binding partners. It is well established that Prxs can interact with multiple proteins in cells, such as ASK1, Cdk5-p35, JNK, MIF, PDGF, TKR4 and others. In this study, we attempted to evaluate a possible association between ubiquitous Prx II and ATP/ADP buffering enzyme - brain-type creatine kinase (CKBB). Our co-immunoprecipitation (Co-IP) results from the A549 and HeLa cell lysates with overexpressed HA-Prx II and Flag-CKBB have demonstrated strong association between two proteins under non-stressed conditions. This protein interaction was enhanced by the heat treatment with further HA-Prx IIprecipitation to the immobilized Flag-CKBB depending on the temperature increase. Temperature induced oligomerization of Prx II may contribute to the formation of Prx II conglomerates, which in turn, can associate with CKBB and increase signal intensities on the blotted membranes. Thus, such association and oligomerization of Prx II could take part in recovery and protection of the CKBB enzyme activity from inactivation during heat-induced stress
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Type of document : Magazine article
Edition cipher :
Author(s) : Olkhovych N. V.
Title : Chitotriosidase activity as additional biomarker in the diagnosis of lysosomal storage diseases
Parallel titles :Хітотріозидазна активність як додатковий біомаркер у діагностиці лізосомних хвороб накопичення
Place of publication : Український біохімічний журнал. - 2016. - Т. 88, № 1. - С. 69-78 (Cipher УУ1/2016/88/1)
Notes : Бібліогр.: в кінці ст.
MeSH-main: ЛИЗОСОМАЛЬНОГО НАКОПЛЕНИЯ БОЛЕЗНИ, НЕРВНАЯ СИСТЕМА -- LYSOSOMAL STORAGE DISEASES, NERVOUS SYSTEM
БИОЛОГИЧЕСКИЕ МАРКЕРЫ -- BIOLOGICAL MARKERS
НИМАНА-ПИКА БОЛЕЗНИ -- NIEMANN-PICK DISEASES
МАКРОФАГИ -- MACROPHAGES
ЦИТОЛОГИЧЕСКИЕ МЕТОДЫ -- CYTOLOGICAL TECHNIQUES
Annotation: To date, several genetic variants that lead to a deficiency of chitotriosidase activity have been described. The duplication of 24 bp (dup24bp) in exon 10 of the CHIT1 gene, which causes a complete loss of enzymatic activity of the gene product, is the most common among the European population. The aim of the study was to evaluate the possibility of using chitotriosidase activity as an additional biomarker in diagnosis of lysosomal storage diseases (LSDs) in Ukraine, to determine this parameter in blood plasma of the patients with various lysosomal diseases and to assess the effect of the presence of dup24bp in the CHIT1 gene on this parameter. It has been shown that chitotriosidase activity in blood plasma is a convenient additional biochemical marker in the diagnosis of some LSDs, namely Gaucher disease, Niemann-Pick disease A, B, C and GM1-gangliosidosis. Reference ranges of the normal chitotriosidase activity were determined in blood plasma of Ukrainian population and found to be 8.0-53.1 nmol 4-methylumbelliferone/hml of plasma. The total allele frequency of the dup24bp in the CHIT1 gene in Ukrainian population was determined, which amounted to 0.26 (323/1244) that is higher than in European population. It was indicated that molecular- genetic screening of dup24bp in the CHIT1 gene is a necessary stage in a protocol for the laboratory diagnosis of Gaucher disease, Niemann-Pick disease A, B, C as well as GM1-gangliosidosis to avoid incorrect diagnosis
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Type of document : Magazine article
Edition cipher :
Author(s) : Gudkova O. O., Latyshko N. V., Shandrenko S. G.
Title : Amine oxidases as important agents of pathological processes of rhabdomyolysis in rats
Parallel titles :Роль аміноксидаз за розвитку рабдоміолізу в щурів
Place of publication : Український біохімічний журнал. - 2016. - Т. 88, № 1. - С. 79-87 (Cipher УУ1/2016/88/1)
Notes : Бібліогр.: в кінці ст.
MeSH-main: МОДЕЛИ НА ЖИВОТНЫХ -- MODELS, ANIMAL
БИОГЕННЫЕ АМИНЫ -- BIOGENIC AMINES
МОНОАМИНОКСИДАЗА -- MONOAMINE OXIDASE
ОКСИДАТИВНЫЙ СТРЕСС -- OXIDATIVE STRESS
РАБДОМИОЛИЗ -- RHABDOMYOLYSIS
РЕНАЛЬНАЯ НЕДОСТАТОЧНОСТЬ -- RENAL INSUFFICIENCY
Annotation: In this study we have tested an idea on the important role of amine oxidases (semicarbazide-sensitive amine oxidase, diamine oxidase, polyamine oxidase) as an additional source of oxidative/carbonyl stress under glycerol-induced rhabdomyolysis, since the enhanced formation of reactive oxygen species and reactive carbonyl species in a variety of tissues is linked to various diseases. In our experiments we used the sensitive fluorescent method devised for estimation of amine oxidases activity in the rat kidney and thymus as targeted organs under rhabdomyolysis. We have found in vivo the multiple rises in activity of semicarbazide-sensitive amine oxidase, diamine oxidase, polyamine oxidase (2-4.5 times) in the corresponding cell fractions, whole cells or their lysates at the 3-6th day after glycerol injection. aberrant antioxidant activities depended on rhabdomyolysis stage and had organ specificity. Additional treatment ofanimals with metal chelator ‘Unithiol ’ adjusted only the activity of antioxidant enzymes but not amine oxidases in both organs. Furthermore the in vitro experiment showed that Fenton reaction (hydrogen peroxide in the presence of iron) products alone had no effect on semicarbazide-sensitive amine oxidase activity in rat liver cell fraction whereas supplementation with methylglyoxal resulted in its significant 2.5-fold enhancement. Combined action of the both agents had additive effect on semicarbazide-sensitive amine oxidase activity. We can assume that biogenic amine and polyamine catabolism by amine oxidases is upregulated by oxidative and carbonyl stress factors directly under rhabdomyolysis progression, and the increase in catabolic products concentration contributes to tissue damage in glycerol-induced acute renal failure and apoptosis stimulation in thymus
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10.

Type of document : Magazine article
Edition cipher :
Author(s) : Korneeva K. L., Rodriguez R. R., Ralchenko S. V., Martunovska O. V., Frolova A. О., Martsenyuk O. P., Manzhula L. V., Melnyk V. T., Shkoropad O. Y., Obolenska M. Yu.
Title : Expression of genes, encoding the enzymes of cysteine metabolism in human placenta in the first and third trimesters of uncomplicated pregnancy
Parallel titles :Eкспресія генів, що кодують ензими метаболізму цистеїну в плаценті людини в першому і третьому триместрах неускладненої вагітності
Place of publication : Український біохімічний журнал. - 2016. - Т. 88, № 1. - С. 88-98 (Cipher УУ1/2016/88/1)
Notes : Бібліогр.: в кінці ст.
MeSH-main: ГЕННАЯ ЭКСПРЕССИЯ -- GENE EXPRESSION
ПЛАЦЕНТА -- PLACENTA
БЕРЕМЕННОСТИ ФИЗИОЛОГИЧЕСКИЕ МЕХАНИЗМЫ ПОДДЕРЖАНИЯ -- PREGNANCY MAINTENANCE
ЧЕЛОВЕК -- HUMANS
ФЕРМЕНТЫ -- ENZYMES
ЦИСТЕИН -- CYSTEINE
Annotation: The cellular cysteine is highly regulated in a narrow range of concentrations due to its cyto- and neurtoxicity when it is overwhelmed or its deficiency for protein synthesis and other vital metabolic reactions when its amount is restricted. The regulation of cysteine content and its metabolic products, glutathione, taurine and inorganic sulfur compounds, is scarcely explored in human placenta though cysteine metabolism is closely related to the maintenance of redox status and protection from free radical oxidation, elimination of homocysteine and detoxification. These processes are particularly important for placenta which meets substantial changes of oxygen supply during its development, and is the last metabolically active organ between mother and fetus. The abundance of CDo, CSAD, ADo, SUoX, GCLC and GCLM mRNAs was estimated by RT-qPCR and compared with the computationally analyzed microarray gene expression data from GEo, while the level of individual protein - by western-blot analysis, both in placental samples from first and third trimesters of uncomplicated pregnancies. The abundance of CDO mRNA is significantly up-regulated at term compared to the first trimester, the level of GCLM and GCLC mRNAs remains almost unchanged while the abundance of other mRNAs reduces to varying degrees. overall, the changes of gene expression in third trimester in comparison to the first one estimated by RT-qPCR and microarray coincide while the former data are more informative for the limited group of genes. The data provide the basis for further research of these genes expression and phenotype of human placenta in health and disease
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11.

Type of document : Magazine article
Edition cipher :
Author(s) : Chekhun V. F., Lozovska Yu. V., Burlaka A. P., Ganusevich I. I., Shvets Yu. V., Lukyanova N. Yu., Todor I. M., Tregubova N. A., Naleskina L. A.
Title : Remodulating effect of doxorubicin on the state of iron-containing proteins, and redox characteristics of tumor with allowance for its sensitivity to cytostatic agents
Parallel titles :Ремодулювальний вплив доксорубіцину на кількісні показники залізовмісних протеїнів та редоксзалежні характеристики пухлини з урахуванням її чутливості до цитостатика
Place of publication : Український біохімічний журнал. - 2016. - Т. 88, № 1. - С. 99-108 (Cipher УУ1/2016/88/1)
Notes : Бібліогр.: в кінці ст.
MeSH-main: БОЛЕЗНЬ, МОДЕЛИ НА ЖИВОТНЫХ -- DISEASE MODELS, ANIMAL
КАРЦИНОМА 256 УОКЕРА -- CARCINOMA 256, WALKER
ДОКСОРУБИЦИН -- DOXORUBICIN
ФЕРРИТИНЫ -- FERRITINS
ТРАНСФЕРРИНЫ -- TRANSFERRINS
КИСЛОРОДА АКТИВНЫЕ ФОРМЫ -- REACTIVE OXYGEN SPECIES
Annotation: The study was aimed at determining the changes of metal-containing proteins in blood serum and tumor tissue of animals with parental and doxorubicin-resistant strains of Walker-256 carcinosarcoma before and after the cytostatic administration. It has been shown that upon doxorubicin action the levels of total iron and transferrin in the tissues from the both groups of animals decreased while that offerritine simultaneously increased with more pronounced pattern in the group of animals with resistant tumor strain. It has been shown that upon the action of doxorubicin in tumor tissue of animals with different sensitivity to the cytostatic there could be observed oppositely directed changes in the redox state of these cells that in turn determined the content of “free iron” complexes, ROS generation and concentration of active forms of matrix metalopro- teinase-2 and matrix metaloproteinase-9, namely, the increase of these indexes in animals with parental strain and their decrease in animals with the resistant one. So, our study has demonstrated the remodulating effect of doxorubicin on the state of metal-containing proteins and redox characteristics of tumor dependent on its sensitivity to cytostatic, at the levels of the tumor and an organism. These data may serve as a criterion for the development of programs for the correction of malfunction of iron metabolism aimed at elevating tumor sensitivity to cytostatic agents
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12.

Type of document : Magazine article
Edition cipher :
Author(s) : Sokolik V. V., Koliada O. K., Shulga S. M.
Title : Effect of β-amyloid peptide 42 on the dynamics of expression and formation of Аβ(40), IL-1β, TNFα, IL-6, IL-10 by peripheral blood mononuclear cells in vitro and its correction by curcumin
Parallel titles :Вплив β-амілоїдного пептиду 42 на динаміку експресії і утворення Аβ(40), IL-1β, TNFα, IL-6, IL-10 мононуклеарами периферійної крові in vitro та його корекція куркуміном
Place of publication : Український біохімічний журнал. - 2016. - Т. 88, № 1. - С. 109-118 (Cipher УУ1/2016/88/1)
Notes : Бібліогр.: в кінці ст.
MeSH-main: АМИЛОИДА БЕТА-ПЕПТИДЫ -- AMYLOID BETA-PEPTIDES
КУРКУМИН -- CURCUMIN
МОНОНУКЛЕАРНАЯ ФАГОЦИТАРНАЯ СИСТЕМА -- MONONUCLEAR PHAGOCYTE SYSTEM
ЦИТОКИН-ИНДУЦИРОВАННЫЕ КЛЕТКИ-КИЛЛЕРЫ -- CYTOKINE-INDUCED KILLER CELLS
ЗДОРОВЫЕ ДОБРОВОЛЬЦЫ -- HEALTHY VOLUNTEERS
Annotation: The toxic effect of Αβ-oligomers accompanies chronic inflammation, with cytokines as main mediators. Therefore, the cytokine link of inflammation becomes a new target on the way to restrain amyloidosis. The aim of the study was the effect ofaggregated Аβ42 on the dynamics of expression andformation of endogenous Αβ and cytokines (IL-φ, TNFα, IL-6, IL-10) by peripheral blood mononuclear cells in vitro and its correction by curcumin. A suspension of mononuclear cells isolated ex tempore using ficoll-urografin gradient from venous blood samples of healthy volunteers were used to study the effects of Αβ (15 nM), curcumin (54pM), and their combined action (at similar concentrations) in time dynamics: 0, 1, 3, 6 and 24 h incubation at 37 °C. Polymerase chain reaction with appropriate primers was used to determine the relative expression of mRNA for ΑβΡΡ, TNFa, IL-Ιβ, IL-6, IL-10 and enzyme-linked immunosorbent assay - to determine the content of Αβ40 and cytokines in mononuclear suspension during all periods of incubation. The individual dynamics ΑβΡΡ and cytokine expression was shown under the action of the Αβ2 which had influence on the content of Αβ0 TNFa, IL-1ff IL-6 and IL-10 in mononuclear suspension. Curcumin displayed the inhibitory effect on gene expression of ΑβΡΡ, TNFa and IL6, which resulted in the decrease of the level of these two cytokines and Αβ40. Thus, the dynamics of anti-inflammatory effect of curcumin in vitro for transcriptional and translational levels of cytokine’s formation by mononuclear cells was shown in the work. Direct inhibitory effect of curcumin on the concentration of endogenous Αβ40 during the 24 h incubation in conditions of toxic action of Αβ aggregates was established
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13.

Type of document : Magazine article
Edition cipher :
Author(s) : Bazalii A. V., Horak I. R., Pasichnyk G. V., Komisarenko S. V., Drobot L. B.
Title : Transcriptional regulation of NOX genes expression in human breast adenocarcinoma MCF-7 cells is modulated by adaptor protein Ruk/CIN85
Parallel titles :Регулювання експресії генів NOX на рівні транскрипції в аденокарциномних клітинах грудної залози людини лінії MCF-7 модулюється за участю адаптерного протеїну Ruk/CIN85
Place of publication : Український біохімічний журнал. - 2016. - Т. 88, № 1. - С. 119-125 (Cipher УУ1/2016/88/1)
Notes : Бібліогр.: в кінці ст.
MeSH-main: ТРАНСКРИПЦИЯ ГЕНЕТИЧЕСКАЯ -- TRANSCRIPTION, GENETIC
АДЕНОКАРЦИНОМА -- ADENOCARCINOMA
ПЕПТИДЫ И БЕЛКИ СИГНАЛЬНЫЕ МЕЖКЛЕТОЧНЫЕ -- INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS
ЧЕЛОВЕК -- HUMANS
НАДH-, НАДФH-ЗАВИСИМЫЕ ОКСИДОРЕДУКТАЗЫ -- NADH, NADPH OXIDOREDUCTASES
ВЕСТЕРН-БЛОТТИНГ -- BLOTTING, WESTERN
Annotation: NADPH oxidases are key components of redox-dependent signaling networks involved in the control of cancer cell proliferation, survival and invasion. The data have been accumulated that demonstrate specific expression patterns and levels of NADPH oxidase homologues (NoXs) and accessory genes in human cancer cell lines and primary tumors as well as modulation of these parameters by extracellular cues. our previous studies revealed that RDSproduction by human colorectal adenocarcinoma HT-29 cells is positively correlated with adaptor protein Ruk/CIN85 expression while increased levels of Ruk/CIN85 in weakly invasive human breast adenocarcinoma MCF-7 cells contribute to their malignant phenotype through the constitutive activation of Src/Akt pathway. In this study, to investigate whether overexpression of Ruk/CIN85 in MCF-7 cells can influence transcriptional regulation of NOXs genes, the subclones of MCF-7 cells with different levels of Ruk/CIN85 were screened for NoX1, NoX2, NoX3, NoX4, NoX5, DUoXl and DUoX2 as well as for regulatory subunitp22Phox mRNA contents by quantitative RT-PCR (qPCR). Systemic multidirectional changes in mRNA levels for NoXl, NoX2, NoX5, DUoX2 and p22Phox were revealed in Ruk/CIN85 overexpressing cells in comparison to control WT cells. Knocking down of Ruk/CIN85 using technology of RNA-interference resulted in the reversion of these changes. Further studies are necessary to elucidate, by which molecular mechanisms Ruk/CIN85 could affect transcriptional regulation of NoXs genes
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14.

Type of document : Magazine article
Edition cipher :
Author(s) : Данилова В. М., Черниш І. Г., Виноградова Р. П., Луговська Г. Г., Юрасова С. П.
Title : Аналіз винахідницької діяльності відділу біохімії вітамінів і коензимів Інституту біохімії ім. О. В. Палладіна НАН України в 1980–2015 рр.
Parallel titles :Analysis of inventive activity of the department of vitamin and coenzyme biochemistry of Palladin Institute of Biochemistry NAS of Ukraine in the period of 1980-2015
Place of publication : Український біохімічний журнал. - 2016. - Т. 88, № 1. - С. 126-140 (Cipher УУ1/2016/88/1)
Notes : Бібліогр.: в кінці ст.
MeSH-main: АКАДЕМИИ И ИНСТИТУТЫ -- ACADEMIES AND INSTITUTES
БИОМЕДИЦИНСКИЕ ИССЛЕДОВАНИЯ -- BIOMEDICAL RESEARCH
КОФЕРМЕНТЫ -- COENZYMES
ВИТАМИНЫ -- VITAMINS
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