Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Circulating tumor cells in breast cancer : functional heterogeneity, pathogenetic and clinical aspects / N. V. Cherdyntseva [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P2-11 : il. - 84 ref.

MeSH-головна:
МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS
ЛЕКАРСТВЕННАЯ ТЕРАПИЯ -- DRUG THERAPY
НОВООБРАЗОВАНИЙ ЦИРКУЛИРУЮЩИЕ КЛЕТКИ -- NEOPLASTIC CELLS, CIRCULATING
БИОПСИЯ -- BIOPSY
Анотація: Each patient has a unique history of cancer ecosystem development, resulting in intratumor heterogeneity. In order to effectively kill the tumor cells by chemotherapy, dynamic monitoring of driver molecular alterations is necessary to detect the markers for acquired drug resistance and find the new therapeutic targets. To perform the therapeutic monitoring, frequent tumor biopsy is needed, but it is not always possible due to small tumor size or its regression during the therapy or tumor inaccessibility in advanced cancer patients. Liquid biopsy appears to be a promising approach to overcome this problem, providing the testing of circulating tumor cells (CTC) and/or tumor-specific circulating nucleic acids. Their genomic characteristics make it possible to assess the clonal dynamics of tumors, comparing it with the clinical course and identification of driver mutation that confer resistance to therapy. The main attention in this review is paid to CTC. The biological behavior of the tumor is determined by specific cancerpromoting molecular and genetic alterations of tumor cells, and by the peculiarities of their interactions with the microenvironment that can result in the presence of wide spectrum of circulating tumor clones with various properties and potentialities to contribute to tumor progression and response to chemotherapy and prognostic value. Indeed, data on prognostic or predictive value of CTC are rather contradictory, because there is still no standard method of CTC identification, represented by different populations manifesting various biological behavior as well as different potency to metastasis. Circulating clasters of CTC appear to have essentially greater ability to metastasize in comparison with single CTC, as well as strong association with worse prognosis and chemoresistance in breast cancer patients. The Food and Drug Administration (USA) has approved the CTC-based prognostic test for clinical application in patients with advanced breast cancer. Prospective clinical trials have demonstrated that measuring changes in CTC numbers during treatment is useful for monitoring therapy response in breast cancer patients. Molecular and genetic analysis of CTC gives the opportunity to have timely information on emergence of resistant tumor clones and may shed light on the new targets for pathogenetic antitumor therapy
Дод.точки доступу:
Cherdyntseva, N.V.
Litviakov, N.V.
Denisov, E.V.
Gervas , P.A.
Cherdyntsev, E.S.

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Kashuba, E.
Do MRPS 18-2 and RB proteins cooperate to control cell stemness and differentiation, preventing cancer development? / E. Kashuba, M. Mushtag // Experimental Oncology. - 2017. - Том 39, N 1. - P12-16 : цв. ил. - 21 ref.

MeSH-головна:
МИТОХОНДРИАЛЬНЫЕ БЕЛКИ -- MITOCHONDRIAL PROTEINS
РЕТИНОБЛАСТОМЫ БЕЛОК -- RETINOBLASTOMA PROTEIN
СТВОЛОВЫЕ КЛЕТКИ -- STEM CELLS
Анотація: In childhood tumors, including retinoblastoma, osteosarcoma, and neuroblastoma, the RB-E2F1 pathway is inactivated, as a rule. These tumors arise from precursor cells that fail to undergo the terminal differentiation. Noteworthy, the RB1-encoded protein (RB) does not control the cell cycle in embryonic stem cells. It has not been yet well understood how RB controls cell stemness and differentiation. The question arises why “inactive” RB is required for the survival and stemness of cells? Recently, we have found that overexpression of the RB-binding protein MRPS18-2 (S18-2) in primary fibroblasts leads to their immortalization, which is accompanied by the induction of embryonic stem cell markers and, eventually, malignant transformation. We suggest that cell stemness may be associated with high expression levels of both proteins, RB and S18-2. There must be a strict regulation of the expression levels of S18-2 and RB during embryogenesis. Disturbances in the expression of these proteins would lead to the abnormalities in development. We think that the S18-2 protein, together with the RB, plays a crucial role in the control on cell stemness and differentiation. We hope to uncover the new mechanisms of the cell fate determination. The S18-2 may serve as a new target for anticancer medicines, which will help to improve human health
Дод.точки доступу:
Mushtag, M.

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Effect of antitumor drugs in low concentrations on the biological, immunophenotypic and cytogenetic characteristics of human colon cancer cells in vitro / N. Bezdieniezhnych [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P17-24 : цв. ил. - 27 ref.

MeSH-головна:
ОБОДОЧНОЙ КИШКИ НОВООБРАЗОВАНИЯ -- COLONIC NEOPLASMS (лекарственная терапия)
ПРОТИВООПУХОЛЕВЫЕ СРЕДСТВА -- ANTINEOPLASTIC AGENTS
ЭПИТЕЛИАЛЬНО-МЕЗЕНХИМНЫЙ ПЕРЕХОД -- EPITHELIAL-MESENCHYMAL TRANSITION (генетика)
Анотація: To estimate the impact of the low-dose anticancer drugs (ACD) with the different mechanisms of action and human interferon (IFN) alpha 2b on the biological properties, immunophenotypic and cytogenetic characteristics of colon cancer cells in vitro. Materials and Methods: The study was performed on human colon cancer cell lines COLO 205, HT-29 and 3C-P treated with ACD and IFN in subtoxic concentrations. Expression of CD44, N-cadherin, vimentin, β-catenin, ERCC1 and Slug was assessed by immunocytochemical method. Using cytogenetic analysis, the numbers of mitoses, cells with micronuclei, apoptotic cells and cells with nuclear protrusions were studied. Results: The prolonged exposure (up to 30 days) of colon cancer cells to low-dose ACD (0.2–0.5 µg/ml cisplatin and 0.1–0.2 µg/ml irinotecan) in combination with IFN (500–1000 IU/ml) led to 37-fold decreased colony-forming activity of these cell and 10-fold reduction of the number of cells expressing mesenchymal protein markers (N-cadherin, vimentin). Also, in COLO 205 cells treated with ACD and IFN the number of SLUG- and CD44-positive cells decreased by 92 and by 85%, respectively. Long-term cultivation of HT-29 cells in the presence of cisplatin and IFN resulted in 5-fold suppression of ERCC1 expression. The cytogenetic analysis has shown that the ACD, IFN and their combinations in subtoxic concentrations caused significant genotoxic effect, suppression of cell proliferation and accumulation of cells with micronuclei. The sensitivity of colon cancer cells to ACD in standard cytotoxic concentrations did not change after prolonged low-dose exposure. Conclusion: The data showed that the prolonged action of the low doses of ACD on human colon cancer cells resulted in the suppression of cell proliferation, colony-forming activity in soft agar, expression of epithelialmesenchymal transition-associated markers and significant cytogenetic changes
Дод.точки доступу:
Bezdieniezhnych, N.
Kovalova, O.
Lykhova, O.
Kocherga, R.
Vorontsova, A.
Zhylchuk, V.
Maksimyak, G.
Kudryavets, Yu.

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Artemisinin modulating effect on human breast cancer cell lines with different sensitivity to cytostatics / V. F. Chekhun [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P25-29 : табл. - 21 ref.

MeSH-головна:
МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS (лекарственная терапия)
АРТЕМИЗИНИНЫ -- ARTEMISININS
ЛЕКАРСТВЕННАЯ УСТОЙЧИВОСТЬ -- DRUG RESISTANCE
Анотація: To explore effects of Artemisinin on a series of breast cancer cells with different sensitivity to typical cytotoxic drugs (doxorubicin — Dox; cisplatin — DDP) and to investigate possible artemisinin-induced modification of the mechanisms of drug resistance. Materials and Methods: The study was performed on wild-type breast cancer MCF-7 cell line (MCF-7/S) and its two sublines MCF-7/Dox and MCF-7/DDP resistant to Dox and DDP, respectively. The cells were treated with artemisinin and iron-containing magnetic fluid. The latter was added to modulate iron levels in the cells and explore its role in artemisinin-induced effects. The MTT assay was used to monitor cell viability, whereas changes of expression of selected proteins participating in regulation of cellular iron homeostasis were estimated using immunocytochemical methods. Finally, relative expression levels of miRNA-200b, -320a, and -34a were examined by using qRT-PCR. Results: Artemisinin affects mechanisms of the resistance of breast cancer cells towards both Dox and DDP at sub-toxic doses. The former drug induces changes of expression of iron-regulating proteins via different mechanisms, including epigenetic regulation. Particularly, the disturbances in ferritin heavy chain 1, lactoferrin, hepcidin (decrease) and ferroportin (increase) expression (р ≤ 0.05) were established. The most enhanced increase of miRNA expression under artemisinin influence were found for miRNA-200b in MCF-7/DDP cells (7.1 ± 0.98 fold change), miRNA-320a in MCF-7/Dox cells (2.9 ± 0.45 fold change) and miRNA-34a (1.7 ± 0.15 fold change) in MCF-7/S cells. It was observed that the sensitivity to artemisinin can be influenced by changing iron levels in cells. Conclusions: Artemisinin can modify iron metabolism of breast cancer cells by its cytotoxic effect, but also by inducing changes in expression of iron-regulating proteins and microRNAs (miRNAs), involved in their regulation. This modification affects the mechanisms that are implicated in drug-resistance, that makes artemisinin a perspective modulator of cell sensitivity towards chemotherapeutic agents in cancer treatment
Дод.точки доступу:
Chekhun, V.F.
Lukianova, N.Y.
Borikun, T.V.
Zadvornyi, T.V.
Mokhir, A.

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Parthenolide reduces gene transcription of prosurvival mediators in U937 cells / S. Mohammadi [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P30-35 : il. - 55 ref.

MeSH-головна:
ОСТЕОПОНТИН -- OSTEOPONTIN
ТРАНСКРИПЦИИ ФАКТОР RELB -- TRANSCRIPTION FACTOR RELB
БЕТА-КАТЕНИН -- BETA CATENIN
Анотація: In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway or β-catenin have been considered. Aim: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN and β-catenin genes. Materials and Methods: The U937 cells were treated with PTL at concentrations of 4 μM (IC25) or 6 μM (IC50) and with OPN siRNA for MTT assay and colony forming assay. Western blot analysis using antibodies against OPN was performed with lysates of PTL-treated cells. Quantitative real-time polymerase chain reaction was performed using primers for OPN siRNA, RelB, AKT1, mTOR, PTEN and β-catenin. Results: PTL reduces OPN protein level and down-regulates RelB mRNA in U937 cell line. Suppression of OPN with siRNA increases the cytotoxic effects of PTL. Also, mRNA expression of AKT1, mTOR, PTEN, and β-catenin decreases with PTL or OPN siRNA. Conclusion: Sensitivity of U937 cells to PTL can be associated with the reduction in expression of prosurvival mediators
Дод.точки доступу:
Mohammadi, S.
Zahedpanah, M.
Nikbakht, M.
Shaiegan, M.
Hamidollah Ghaffari, S.
Nikugoftar, M.
Rahmani, B.
Asl, D.

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Anti-histone H1 IgGs prossess proliferative activity towards human T-leukemia CEM cells / Yu. Kit [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P36-41 : il. - 34 ref.

MeSH-головна:
ЛЕЙКОЗ T-КЛЕТОЧНЫЙ -- LEUKEMIA, T-CELL
ВОЛЧАНКА КРАСНАЯ СИСТЕМНАЯ -- LUPUS ERYTHEMATOSUS, SYSTEMIC
КРОВИ СЫВОРОТКА -- SERUM
КЛЕТКИ ПРОЛИФЕРАЦИЯ -- CELL PROLIFERATION
ПЕРЕКРЕСТНЫЕ РЕАКЦИИ -- CROSS REACTIONS
АУТОАНТИТЕЛА -- AUTOANTIBODIES
Анотація: The aim of this study was to characterize the proliferative activity of the anti-histone H1 IgGs towards human T-leukaemia CEM cells. Materials and Methods: Anti-histone H1 IgGs were purified from blood serum of systemic lupus erythematosus patients by precipitation of serum proteins with 50% ammonium sulfate followed by a sequential affinity chromatography on Protein GSepharose and histone H1-Sepharose columns. To avoid contamination with other proteins, anti-histone H1 IgGs were subjected to strongly acidic pH 2.0 during gel filtration through HPLC column. The effects of the anti-histone H1 IgGs on cell viability and cell cycle were tested by MTS-assay and flow cytometry, correspondingly. The cross-reactivity of the anti-histone H1 antibodies towards heterogenetic and cellular antigens was evaluated by Western-blot analysis. Results: It was found that incubation of CEM cells with the HPLC-purified anti-histone H1 IgGs resulted in significant stimulation of cell growth by 46% after 48 h of incubation. These IgGs possess an antigenic poly-specificity to positively charged heterogenetic antigens and different cellular antigens. FITC-labeled and biotinylated anti-histone H1 IgGs are internalized by CEM cells and preferentially accumulated in the cytoplasm. Conclusion: The anti-histone H1 IgGs are shown to internalize human T-leukemia CEM and stimulate their proliferation. These IgGs are polyspecific toward cellular antigens
Дод.точки доступу:
Kit, Yu.
Magorivska, I.
Bilyi, R.
Myronovskij, S.
Stoika, R.

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Anticancer effect and immunologic response to xenogeneic embryonic proteins in mice bearing Ehrlich solid carcinoma / T. V. Symchych [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P42-48 : табл. - 27 ref.

MeSH-головна:
КАРЦИНОМА, ЭРЛИХА ОПУХОЛЬ -- CARCINOMA, EHRLICH TUMOR (терапия)
ВАКЦИНЫ ПРОТИВООПУХОЛЕВЫЕ -- CANCER VACCINES (иммунология)
Анотація: To investigate anticancer and immunologic effects of chicken embryonic proteins (CEP) in mice bearing Ehrlich solid carcinoma. Materials and Methods: The study was carried out on male Balb/c mice bearing Ehrlich solid carcinoma. The immunizations were performed after the tumor transplantation. The immune status was assessed on days 7, 14, 21 and 28 after the tumor challenge. Cytotoxic activity (CAT) of macrophages (Mph), natural killer cells (NK), cytotoxic T-lymphocytes (CTL) and blood serum, as well as the influence of the blood serum on immune cells activity was checked in MTT-assay; Mph’s cytochemical activity was tested in NBT-assay; Ehrlich antigen-specific or CEP-specific antibodies were detected in ELISA-assay; medium size circulating immune complexes (CIC) were detected in reaction of 4.5% polyethylene glycol precipitation. Results: The immunization resulted in tumor growth suppression and significant 25.64% prolongation of the survival time. In both control and immunized mice with transplanted tumors antibodies specific to Ehrlich carcinoma antigens and to CEP were detected, but antibody response was more balanced in the treatment group. In the treatment group both cytochemical and CAT of Mph was moderately activated and well preserved until late stages of tumor development; CAT of NK and CTL remained in the range of the intact mice until day 28 after the tumor transplantation. The immunized mice were well protected from accumulation of CIC and suppressive activity of autologous blood serum. Conclusion: Collectively, our data indicate that CEP can elicit immunomodulating and immunoprotecting effects sufficient to provide tumor growth inhibition. The further elaboration of a xenogeneic anticancer vaccine based on CEP is warranted
Дод.точки доступу:
Symchych, T.V.
Fedosova, N.I.
Karaman, O.M.
Yevstratieva, L.M.
Voyeykova, I.M.
Potebnia, H.P.

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EPR spectroscopy studies of changes in erythrocyte membranes in patients with laryngeal cancer / Y. B. Burlaka [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P49-52 : табл. - 18 ref.

MeSH-головна:
ГОРТАНИ НОВООБРАЗОВАНИЯ -- LARYNGEAL NEOPLASMS (диагностика)
ЭРИТРОЦИТЫ -- ERYTHROCYTES
ЭРИТРОЦИТА МЕМБРАНА -- ERYTHROCYTE MEMBRANE
ЭЛЕКТРОННОГО ПАРАМАГНИТНОГО РЕЗОНАНСА СПЕКТРОСКОПИЯ -- ELECTRON SPIN RESONANCE SPECTROSCOPY
Анотація: To evaluate microviscosity and sorption capacity of erythrocyte membranes (SCEM) from patients with laryngeal cancer (LC). Materials and Methods: Samples from 35 patients with LC of stages II and III and 20 healthy volunteers were investigated by electron paramagnetic resonance with Bis(1-oxyl-2,2,6,6-tetramethylpiperidinyl-4)-ester of 5,7-dimethyladamantane-1,3-dicarbonic acid (AdTEMPO) probe. SCEM was evaluated by amount of unabsorbed methylene blue. Results: Microviscosity of erythrocyte membranes was determined by the effective rotational diffusion correlation times (τeff) and a decrease in radical spectrum signal intensity per hour. The most apparent decrease in mobility of the AdTEMPO in erythrocytes was observed prior to washing of erythrocytes with 0.9% NaCl for 5 min after probe insertion. The deceleration after 60 min was observed only in stage II LC. τeff was at control values after washing of erythrocytes of stage II LC 5 min after probe insertion and was significantly reduced in stage III LC in comparison to control. Radical spectrum signal intensity per hour in samples of stage II and III patients prior to and after washing of erythrocytes was on average 1.5-fold higher than that of control. SCEM in samples of stage II and III LC was found in 40 and 33% cases, respectively and was on average significantly reduced in comparison to control. Conclusions: The initial interaction of AdTEMPO with erythrocyte membranes of stage II and III LC patients is accompanied by an increase in τeff, indicating deceleration of probe rotation. τeff of the probe in membranes remains unchanged in 60 min, indicating changes in the structural organization of lipid bilayer and its associated proteins in particular. The similarity of SCEM for both studied groups reflects the pathological changes in function of erythrocyte membranes
Дод.точки доступу:
Burlaka, Y.B.
Sukhoveev, O.V.
Grin, N.V.
Khilchevskyi, O.M.
Verevka, S.V.

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Auzina, D.
Prognostic value of the bone turnover markers in multiple myeloma / D. Auzina, R. Erts, S. Lejniece // Experimental Oncology. - 2017. - Том 39, N 1. - P53-56 : табл. - 25 ref.

MeSH-головна:
МИЕЛОМА МНОЖЕСТВЕННАЯ -- MULTIPLE MYELOMA (диагностика)
Анотація: Multiple myeloma (MM) is characterized by osteolytic bone disease resulting from increased osteoclast activity and reduced osteoblast function. Aim: The aim of our research was to determine connection between bone turnover markers and presence of bone lesions, their degree of severity, to monitor MM bone disease and to assess effectiveness of anti-myeloma treatment. Materials and Methods: Serum samples and clinical data from 123 patients with newly diagnosed MM were collected at Riga East Clinical University Hospital (Riga, Latvia) from June 2014 to June 2016. Bone lesions detected by radiography, CT scans, MRI, and PET/CT were divided into degrees from 0 to 3 (0 — no bone involvement, 1 — ≤ 3 bone lesions, 2 — ≥ 3 bone lesions, 3 — fracture). Staging was performed applying Durie/Salmon (DS) and International Staging System classifications. Progressive disease was defined as development of one or more new bone lesions. The levels of bone metabolic markers β-isomerized C-terminal telopeptide of collagen type I (β-CTX) and bone-specific alkaline phosphatase (bALP) were monitored regularly in the year. Results: Bone lesions were found in 86 (69%) patients. From these 6 (4%) patients had 1st degree, 11 (9%) had 2nd degree and 69 (56%) had 3rd degree bone lesions. Level of the bone resorption marker β-CTX in the control group was 0.41 ng/ml, which is lower than in MM patients (p 0.001). Spearman correlation coefficient analysis found a positive and statistically significant correlation (rs 0.05). However, β-CTX was found to be an excellent diagnostic marker for MM (AUC 0.91; 95% confidence interval, 0.88–0.94; p 0.001). Conclusions: Patients with MM and bone lesions have increased value of bone resorption marker β-CTX. There is a correlation between bone resorption marker and degree of bone lesions. Changes in β-CTX levels may be used to monitor the effectiveness of myeloma treatment
Дод.точки доступу:
Erts, R.
Lejniece, S.

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10.

Aberrant promoter hypermethylation of selected apoptotic genes in childhood acute lymphoblastic leukemia among North Indian population / M. Nikbakht [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P57-64 : табл. - 51 ref.

MeSH-головна:
ЛЕЙКОЗ-ЛИМФОМА ПРЕ-КЛЕТОЧНЫЙ ЛИМФОБЛАСТНЫЙ -- PRECURSOR CELL LYMPHOBLASTIC LEUKEMIA-LYMPHOMA (генетика)
ЭПИГЕНОМИКА -- EPIGENOMICS
ДНК ФРАГМЕНТАЦИЯ -- DNA FRAGMENTATION
Анотація: Promoter hypermethylation mediates gene silencing in many neoplasms. Acute leukemia has been reported to harbor multiple genes aberrantly silenced by hypermethylation. Aim: In present study, we investigated the prevalence of hypermethylation of caspase-8 (CASP8), TMS1 and DAPK genes in correlation with clinicopathological factors in childhood acute lymphoblastic leukemia (ALL). Materials and Methods: A case-control study has been conducted based on bone marrow and peripheral blood samples from 125 ALL patients and 100 sex-age matched healthy controls. Methylation specific polymerase chain reaction (PCR) and bisulfite sequencing PCR was performed to analyze the methylation status of these genes. Reverse transcription PCR and real time PCR was carried out to determine changes in the mRNA expression level of the genes due to hypermethylation. Results: Hypermethylation of the 5´CpG islands of the CASP8, TMS1 and DAPK gene promoters was found in 3.2, 6.4, and 13.6% of 125 childhood ALL samples from north Indian population, respectively. There were significant differences in pattern of hypermethylation of TMS1 (p = 0.045) and DAPK (p 0.001) between patients and healthy controls. Down-regulation of mRNA expression was found in cases in which CASP8, TMS1 and DAPK were hypermethylated. Conclusions: The present study indicated the impact of hypermethylation-mediated inactivation of CASP8, TMS1 and DAPK genes, which is associated with risk of childhood ALL. This abnormality occurs in leukemogenesis and it may be used as a biomarker and for predicting the prognosis of ALL
Дод.точки доступу:
Nikbakht, M.
Jha, A.K.
Malekzadeh, K.
Askari, M.
Mohammadi, S.
Marwaha, R.K.
Kaul, D.
Kaur, J.

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11.

Chekhun, V. F.
Significance of iodine symporter for prognosis of the disease course and efficacy of neoadjuvant chemotherapy in patients with breast cancer of luminal and basal subtypes / V. F. Chekhun, A. V. Andriiv, N. Yu. Lukianova // Experimental Oncology. - 2017. - Том 39, N 1. - P65-68 : табл. - 16 ref.

MeSH-головна:
МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS (лекарственная терапия)
ХИМИОТЕРАПИЯ АДЪЮВАНТНАЯ -- CHEMOTHERAPY, ADJUVANT
СИМПОРТЕРЫ -- SYMPORTERS
Анотація: The aim of the research was to study the relation between expression of Na⁺/I⁻symporter (NIS) in breast cancer (BC) of different molecular subtypes and sensitivity of BC cells to neoadjuvant chemotherapy (NACT) and to assess whether NIS expression may be used as a predictive marker of treatment efficacy. Materials and Methods: The study included 148 women with BC of stage II–III who were treated at the Precarpathian Clinical Oncology Center during 2012–2017. All patients were treated with NACT that included 2–6 cycles of chemotherapy by FAC, AC scheme with 21 day intervals. NACT efficacy was evaluated every 2 cycles by mammography according to RECIST criteria. Morphological and immunohistochemical study of NIS expression was performed by the standard methods on paraffin sections of surgically resected tumors. Results: The heterogeneity of different molecular BC subtypes regarding response to the NACT has been found. Her2/neu-positive and basal BC subtypes were the least susceptible to the NACT (p 0.05). It was shown that NIS expression is related to the sensitivity of luminal B and basal BC subtypes to the NACT. The highest expression of NIS and impairment of its functional activity was registered in the group of patients with tumors resistant to NACT (stabilization of the disease or its progression) of luminal B (220 ± 8.6 points) and basal subtypes (290 ± 11.3 points) (p 0.05). It was revealed that the disease-free survival of patients with BC of luminal B and basal subtypes was higher in the absence of NIS expression in tumor cells (p 0.05). Conclusions: The results indicate that NIS can be used as an objective criterion for predicting the sensitivity of luminal B and basal BC subtypes to NACT, which will provide improved treatment outcomes in this group of patients
Дод.точки доступу:
Andriiv, A.V.
Lukianova, N.Yu.

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12.

Satinder, K.
Impact of single nucleotide polymorphism in chemical metabolizing genes and exposure to wood smoke on risk of cervical cancer in North-Indian women / K. Satinder, R. C. Sobti, K. Pushpinder // Experimental Oncology. - 2017. - Том 39, N 1. - P69-74 : табл. - 33 ref.

MeSH-головна:
МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS (радиотерапия, хирургия)
ВЫЖИВАНИЕ -- SURVIVAL
Анотація: n the present study, we investigated the hypothesis whether exposure to wood smoke increases the risk of cervical cancer (CC) in North-Indian women who inherit different polymorphic forms of chemical metabolizing genes (GSTM1, GSTT1, GSTP1 and CYP1A1). Materials and Methods: One hundred fifty histologically confirmed CC patients and equal number of cancer-free age and ethnicity matched controls were genotyped for genetic polymorphism in chemical metabolizing genes by using polymerase chain reaction/restriction fragment length polymorphism method. The association of the different genotypes and exposure to wood smoke with the risk of CC in North-Indian women was estimated by doing statistical analysis using Statistical Package for the Social Science. Results: It was observed that the variant genotypes of GSTM1, GSTT1, GSTP1 and CYP1A1 did not significantly increase the risk of CC. However, statistically significant increased risk (odds ratio 3.6; 95% confidence interval, 1.34–9.78; p = 0.008) was observed for women who used wood for cooking and had GSTM1 (null) genotype. Conclusions: The present study suggests that genetic differences in the metabolism of wood smoke carcinogens, particularly by GSTM1, may increase the risk of CC
Дод.точки доступу:
Sobti, R.C.
Pushpinder, K.

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13.

The impact of coloregional treatment on survival of patients with primary metastatic breast cancer / R. Liubota [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P75-77 : табл. - 13 ref.

MeSH-головна:
МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS (радиотерапия, хирургия)
ВЫЖИВАНИЕ -- SURVIVAL
Анотація: The aim of the study was to investigate the impact of primary tumor locoregional treatment (surgery or/and radiotherapy) on overall survival in patients with primary metastatic breast cancer (PMBC). Materials and Methods: This retrospective study included 295 wo men aged from 23 to 76 years with PMBC. Among the 295 patients, the effect of locoregional treatment of primary tumor on survival outcomes was evaluated in 177 women with distant metastases at diagnosis of breast cancer. 35 patient received breast surgery (group 1), 95 patients with PMBC — radiotherapy (group 2) and 47 patients — combination of breast surgery and radiation (group 3). The remaining 118 patients didn’t receive surgery or/and radiotherapy (group 4). All patients received systemic cytotoxic chemotherapy. Results: The groups of patients with PMBC did not differ significantly by age, menstrual function, ER status, Her2 receptor status, site of metastasis and number of metastatic lesions. 2- and 5-year overall survival in patients of group 1 was 54 and 32%, group 2 — 47 and 8%, group 3 — 73 and 18%, whereas in patients from group 4 — 26 and 9%, respectively. The median survival of patients who underwent surgery was 36 months, patients with PMBC who received radiotherapy — 24 months, patients who obtained combination of breast surgery and radiation — 30 months vs 18 months in patients who did not undergo primary tumor locoregional treatment. Conclusions: The results of this study showed a favourable effect of locoregional treatment in patients with PMBC
Дод.точки доступу:
Liubota, R.
Cheshuk, V.
Vereshchako, R.
Zotov, O.
Zaichuk, V.
Anikusko, N.
Liubota, I.

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14.

Diagnostic challenges with intraoral myeloid sarcoma : report of two cases & review of world literature / P. Kumar [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P78-85 : цв. ил. - 64 ref.

MeSH-головна:
САРКОМА МИЕЛОИДНАЯ -- SARCOMA, MYELOID (диагностика)
ЛЕЙКОЗЫ -- LEUKEMIA
ГРАНУЛОЦИТЫ -- GRANULOCYTES
ИММУНОГИСТОХИМИЯ -- IMMUNOHISTOCHEMISTRY (методы)
Анотація: Myeloid sarcomas (MS) are rare extramedullary tumors composed of blasts of myeloid lineage that either precede, follow or present concomitantly with acute myeloid leukaemia (AML) or myeloproliferative neoplasms. The diagnosis of MS is especially challenging in patients without an antecedent history of leukemia. Methods: We present 2 cases of intraoral MS that presented as de novo lesions. A detailed review of cases of intraoral MS that either preceded or presented along with leukemia has been done with emphasis on diagnostic criteria used. Results: Two male patients aged 28 and 5 years presented with MS with one patient presenting with concomitant AML. A combination of morphological and immunohistochemical methods was used for diagnosis. A thorough review of world literature revealed 44 cases of intraoral MS that presented as de novo lesions. Conclusion: Intraoral MS is a rare tumor with poor prognosis. It may be diagnostically challenging due to its protean clinical manifestations and histological overlap with other tumors
Дод.точки доступу:
Kumar, P.
Singh, H.
Khurana, N.
Urs, A.B.
Augustine, J.
Tomar, R.

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15.

Trichilemmal cystis in metastatic melanoma : a case report / I. Savarese [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P86-87 : цв. ил. - 8 ref.

MeSH-головна:
МЕЛАНОМА -- MELANOMA
НОВООБРАЗОВАНИЙ МЕТАСТАЗЫ -- NEOPLASM METASTASIS
ПАРАНЕОПЛАСТИЧЕСКИЕ СИНДРОМЫ -- PARANEOPLASTIC SYNDROMES
КИСТЫ -- CYSTS
Анотація: The malignant melanoma is a neoplasm associated with a wide variety of cutaneous paraneoplastic syndromes, as dermatomyositis, systemic sclerosis, paraneoplastic pemphigus. We describe a case of four multiple trichilemmal cystis arising on frontal region in the same patient with brain metastasis and unknown primary melanoma and discuss their relationship
Дод.точки доступу:
Savarese, I.
Grazzini, M.
Gori, A.
D’Errico, A.
Doni, L.
Scarfi, F.
Covarelli, P.
Di Costanzo, F.
De Giorgi, V.

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16.

Materials of international vactrain/ 3-rd Swedish-Ukrainian conference on cancer diseases, January 16-17, 2017, Stockholm, Sweden // Experimental Oncology. - 2017. - Том 39, N 1. - P88-93

MeSH-головна:
ОНКОЛОГИЯ МЕДИЦИНСКАЯ -- MEDICAL ONCOLOGY (тенденции)
НОВООБРАЗОВАНИЯ -- NEOPLASMS
СЪЕЗДЫ, СИМПОЗИУМЫ -- CONVENTIONS, SYMPOSIUMS
Анотація: The scientific conference ‘‘International VACTRAIN/ 3-rd Swedish-Ukrainian conference on cancer diseases’’ was held on January 16–17, 2017 at Karolinska Institutet, Stockholm, Sweden. These 1.5 days were filled with the lectures by the invited speakers and oral presentations selected on the basis of submitted abstracts from younger researchers. The meeting was focused on what we have learnt over the recent times, on the current situation, and on the important challenges for the future with focus on combating cancer
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17.

Endoplasmic reticulum stress as a key factor of genome reprogramming in cancer cells / O. O. Ratushna [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P88-89

MeSH-головна:
НОВООБРАЗОВАНИЯ -- NEOPLASMS
ЭНДОПЛАЗМАТИЧЕСКОГО РЕТИКУЛУМА СТРЕСС -- ENDOPLASMIC RETICULUM STRESS
Анотація: The endoplasmic reticulum (ER) stress represents the unfolded protein response to cope with the accumulation of unfolded or misfolded proteins. It is required to maintain the functional integrity of the ER, which is a dynamic intracellular organelle with exquisite sensitivity to alterations in homeostasis. The unfolded protein response is a key player in the development of different malignant tumors. Depending on the duration and severity of the ER stress, it leads to cell adaptation or demise. This stress is a fundamental phenomenon, which provides a secure protection of the cells from different environmental challenges and is transduced by three major ER resident stress sensors. Activation of these ER stress sensors leads to transcriptional reprogramming of the cells. The signaling pathways elicited by those stress sensors have connections with metabolic pathways and with other plasma membrane receptor signaling networks. As such, the ER has an essential position as a signal integrator in the cell and is instrumental in the different phases of tumor progression
Дод.точки доступу:
Ratushna, O.O.
Minchenko, D.O.
Riabovol, O.O.
Luzina, O.Y.
Minchenko, O.H.

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18.

The adaptor protein Ruk/CIN85 paradoxically enhances EMT of triple negative mouse breast adenocarcinoma 4T1 cells / I. Horak [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P89

MeSH-головна:
АДЕНОКАРЦИНОМА -- ADENOCARCINOMA
МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS
АДАПТЕРНЫЕ БЕЛКИ ВЕЗИКУЛЯРНОГО ТРАНСПОРТА -- ADAPTOR PROTEINS, VESICULAR TRANSPORT
ЭПИТЕЛИАЛЬНО-МЕЗЕНХИМНЫЙ ПЕРЕХОД -- EPITHELIAL-MESENCHYMAL TRANSITION
МЫШИ -- MICE
Анотація: To coordinate cellular responses, cell surface receptors employ receptor-associated adaptor proteins that are composed exclusively of domains and motives involved in intermolecular interactions. The assembling of adaptor proteins-mediated supramolecular complexes is regulated in dynamic and selective fashion, thereby influencing processing of information through signaling networks
Дод.точки доступу:
Horak, I.
Shytikov, D.
Geraschenko, D.
Knopfova, L.
Borsig, L.
Drobot, L.

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19.

Biological characteristics of tumor cells at the different stages of EMT upon exposure to anticancer drugs and cytokines / N. Bezdieniezhnych [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P89-90

MeSH-головна:
ПРОТИВООПУХОЛЕВЫЕ СРЕДСТВА -- ANTINEOPLASTIC AGENTS
ЦИТОКИНЫ -- CYTOKINES (действие лекарственных препаратов)
ЭПИТЕЛИАЛЬНО-МЕЗЕНХИМНЫЙ ПЕРЕХОД -- EPITHELIAL-MESENCHYMAL TRANSITION (действие лекарственных препаратов)
Анотація: Formation of a highly malignant metastatic tumor cell phenotype and resistance to anticancer drugs are associated with the implementation of epithelial- mesenchymal transition (EMT). Thus, monitoring of EMT and the possible inhibition of this process could be helpful to inhibit the tumor progression. It is quite often that anti-tumor treatment is not effective, so new modalities and new targets to combat cancer should be developed. Established cell lines, primary cultures of malignant cells obtained from biopsies or ascites of patients with epithelial cancers (breast, colorectal and ovarian), immunohistochemistry, and statistical methods were used
Дод.точки доступу:
Bezdieniezhnych, N.
Lykhova, O.
Kocherga, R.
Kudryavets, Yu.

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20.

Alternative direction of inhibition of malignant properties in tumor cells in vitro and in vivo by gene therapy with INF-beta gene in recombinant baculovirus vector / O. Lykhova [et al.] // Experimental Oncology. - 2017. - Том 39, N 1. - P90

MeSH-головна:
НОВООБРАЗОВАНИЯ -- NEOPLASMS (терапия)
ГЕННАЯ ТЕРАПИЯ -- GENETIC THERAPY
BACULOVIRIDAE -- BACULOVIRIDAE (генетика)
Анотація: The aim of the study was to investigate the influence of recombinant baculovirus containing the interferon-β gene (rBV/IFN) on phenotypic characteristics of tumor cells in vitro: morphology, growth, cytogenetic characteristics and expression of proteins associated with proliferative activity, cell cycle regulation, epithelial-mesenchymal transition (EMT), invasiveness, and the migration potential
Дод.точки доступу:
Lykhova, O.
Strokovska, L.
Kovaleva, O.
Bezdieniezhnych, N.
Semesiuk, N.
Adamenko, I.
Vorontsova, A.
Kudryavets, Yu.

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(Асоціація ЕБНІТ)