Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Загальна кількість знайдених документів : 8
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Danylovych, G. V.
The biosynthesis of nitric oxide from L-arginine. Nitric oxide formation features and its functional role in mitochondria / G. V. Danylovych, T. V. Bohach, Yu. V. Danylovych // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 1. - P3-24

MeSH-головна:
АЗОТА ОКСИДЫ -- NITROGEN OXIDES (химия)
МИТОХОНДРИИ МЫШЦ -- MITOCHONDRIA, MUSCLE (ультраструктура, физиология)
КЛЕТОЧНАЯ КОМПАРТМЕНТАЦИЯ -- CELL COMPARTMENTATION
ЭУКАРИОТЫ -- EUKARYOTA
Анотація: Modern data on biochemical patterns of nitric oxide biosynthesis in mammal cells from L-arginine in normoxic conditions is described. The attention of the authors is given to the results of the recent years on the structure and regulation features isoforms of NO-synthase. The emphasis is put on the latest conception of the compartmentalization of certain isoforms of these enzymes in cells and on the possibility of the directed transport of nitric oxide in the vascular wall. The central place in the review is devoted to issues on the endogenous formation of NO in mitochondria and its potential physiological significance. Our own results on the identification of NO in mitochondria of the uterine smooth muscle, biochemical characteristics of this process and NO possible role in Са2+ transport regulation by organelles are presented and discussed
Дод.точки доступу:
Bohach, T. V.
Danylovych, Yu. V.

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Calix[4]arene C-956 is effective inhibitor of H+-Ca2+-exchanger in smooth muscle mitochondria / G. V. Danylovych [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 1. - P25-33

MeSH-головна:
КАЛИКСАРЕНЫ -- CALIXARENES (химия)
МИТОХОНДРИИ МЫШЦ -- MITOCHONDRIA, MUSCLE (ультраструктура, физиология)
ЭУКАРИОТЫ -- EUKARYOTA
Анотація: It was shown that calix[4]arene C-956 exhibited a pronounced concentration-dependent (10-100 μM) inhibitory effect on the H+-Ca2+-exchanger of the inner mitochondrial membrane of rat uterine myocytes (Ki 35.1 ± 7.9 μM). The inhibitory effect of calix[4]arene C-956 was accompanied by a decrease in the initial rate (V0) and an increase in the magnitude of the characteristic time (τ1/2) of the ΔрН-induced Са2+ release. At the same time, it did not affect the potential-dependent accumulation of Ca2+ in mitochondria. Thus, the action of calix[4]arene C-956 might be directed on increasing the concentration of Ca ions in the mitochondrial matrix. The calculation of basic kinetic parameters of the Ca2+ transport from isolated organelles (in the case of its non-zero stationary level), based on changes in fluorescence of Ca2+-sensitive dye Fluo-4 AM in mitochondria was performed. The proposed approach can be used for the kinetic analysis of the exponential decrease of the fluorescence response of any probes under the same experimental conditions
Дод.точки доступу:
Danylovych, G. V.
Kolomiets, O. V.
Danylovych, Yu. V.
Rodik, R. V.
Kalchenko, V. I.
Kosterin, S. O.

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Effects of Pistacia atlantica resin oil on the level of VEGF, hydroxyproline, antioxidant and wound healing activity in STZ-induced diabetic rats / B. Shahouzehi [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 1. - P34-41

MeSH-головна:
СМОЛЫ РАСТИТЕЛЬНЫЕ -- RESINS, PLANT (фармакология)
МАСЛА -- OILS (фармакология)
ДИАБЕТИЧЕСКАЯ СТОПА -- DIABETIC FOOT (лекарственная терапия)
РАНЫ ЗАЖИВЛЕНИЕ -- WOUND HEALING (действие лекарственных препаратов)
ЭУКАРИОТЫ -- EUKARYOTA
Анотація: Diabetes mellitus is a chronic metabolic disorder which is followed by many complications. One of these problems following diabetes is diabetic ulcers and 2.5% of diabetic patients develop foot ulcer annually. Natural resources are useful stock for finding traditional treatment. Studies which examined Pistacia atlantica properties showed that it has some beneficial effects including: antimicrobial, antifungal, antioxidant and wound healing. Yet, Pistacia atlantica resin oil effects on diabetic wound have not been examined. Therefore, we evaluate Pistacia atlantica resin oil antioxidant and wound healing activity. Sprague-Dawley male rats were entered to the study and randomly grouped (n = 10) as follows: group 1 – control group – burnt rats which received neither STZ nor Pistacia atlantica resin oil; group 2 – diabetic burnt rats; group 3 – diabetic burnt rats which received 250 µl/day Pistacia atlantica resin oil topically as an ointment for two weeks. At the end of the study antioxidant status, Vascular Endothelial Growth Factor (VEGF) and hydroxyproline contents were examined in the wound area. Our results showed that Pistacia atlantica resin oil has remarkable antioxidant activity in STZ-induced diabetic rats. Also, it promoted VEGF and hydroxyproline contents in the wound area which showed that it increases angiogenesis and collagen turnover in the diabetic wound. We concluded that Pistacia atlantica resin oil can be considered as a new therapeutic agent in diabetic wound healing, and also it is safe, available and cheap treatment of other wounds and skin injuries
Дод.точки доступу:
Shahouzehi, B.
Shabani, M.
Shahrokhi, N.
Sadeghiyan, S.
Masoumi-Ardakani, Y.

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Voloshchuk, O. M.
Activity of the mitochondrial isoenzymes of endogenous aldehydes catabolism under the conditions of acetaminophen-induced hepatitis / O. M. Voloshchuk, G. P. Kopylchuk, Y. I. Mishyna // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 1. - P42-47

MeSH-головна:
АЛЬДЕГИД-ДЕГИДРОГЕНАЗА -- ALDEHYDE DEHYDROGENASE
АЛЬДЕГИДРЕДУКТАЗА -- ALDEHYDE REDUCTASE
ПАРАЦЕТАМОЛ -- ACETAMINOPHEN
МИТОХОНДРИИ ПЕЧЕНИ -- MITOCHONDRIA, LIVER
ПЕЧЕНИ ЛЕКАРСТВЕННОЕ ПОВРЕЖДЕНИЕ -- DRUG-INDUCED LIVER INJURY
ЭУКАРИОТЫ -- EUKARYOTA
Анотація: The research deals with the determination of the activity of aldehyde dehydrogenase (EC 1.2.1.3), aldehyde reductase (EC 1.1.1.21) as well as the content of TBA reactive substances and protein carbonyl derivates in the rat liver cytosolic fraction under the conditions of acetaminophen-induced hepatitis and protein deficiency. The most pronounced decrease in the activity of enzymes utilizing endogenous aldehydes is observed in the liver cytosolic fraction of animals with toxic liver injury maintained under the conditions of alimentary protein deficiency. Meanwhile, the accumulation of TBA reactive substances and protein carbonyl-derivates in the liver cytosolic fraction of animals of this experimental group was established. The accumulation of aldehyde products of lipid and protein oxidative damage on the background of the reduction in the activity of enzymes providing aldehyde catabolism may be considered as a possible mechanism underlying hepatocyte dysfunction under the conditions of toxic damage in protein-deficient animals
Дод.точки доступу:
Kopylchuk, G. P.
Mishyna, Y. I.

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Responsiveness to progesterone and potassium channel blockers 4-aminopyridine, tetraethylammonium and free Ca2+ contentration in spermatozoa of patients with oligozoospermia/leucocytospermia / R. V. Fafula [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 1. - P48-57

MeSH-головна:
КАЛЬЦИЙ -- CALCIUM
СПЕРМАТОЗОИДЫ -- SPERMATOZOA
ПРОГЕСТЕРОН -- PROGESTERONE
КАЛЬЦИЕВЫХ КАНАЛОВ БЛОКАТОРЫ -- CALCIUM CHANNEL BLOCKERS
ОЛИГОСПЕРМИЯ -- OLIGOSPERMIA
ЭУКАРИОТЫ -- EUKARYOTA
Анотація: The present study was undertaken to evaluate [Ca2+]i signals that occur in human sperm cells exposed in vitro to three diverse compounds; progesterone, 4-aminopyridine (a highly effective inducer of hyperactivation in human sperm) and tetraethylammonium. The [Ca2+]i reached after the extracellular calcium treatment was always higher in normozoospermic samples pretreated with progesterone than in pathozoospermic samples pretreated with progesterone. There were no changes in calcium signal in spermatozoa pretreated with progesterone from patients with oligozoospermia and leucocytospermia compared to control samples (without progesterone). [Ca2+]i was always higher in pathozoospermic samples without 4-aminopyridine and always lower in pathozoospermic samples with 4-aminopyridine compared to these values in normozoospermic men. The 2 mM extracellular calcium administration to spermatozoa pretreated with tetraethylammonium did not result in a detectable increase in [Ca2+]i in normo- and pathozoospermic samples. The mechanisms of progesterone-dependent activation of the Ca2+-entry and the functioning of the voltage gated Ca2+-channels of plasmalemma are disturbed in pathologies – there was no increase in the Ca2+ level in the conditions of K+-depolarization (in the presence of inhibitors of K+-channels)
Дод.точки доступу:
Fafula, R. V.
Danylovych, G. V.
Besedina, A. S.
Melnyk, O. V.
Vorobets, Z. D.

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Determination of plasminogen/plasmin system components and indicators of lipoproteins oxidative modification under arterial hypertension / O. I. Yusova [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 1. - P58-67

MeSH-головна:
ПЛАЗМИНОГЕНА АКТИВАТОР ТКАНЕВОГО ТИПА -- TISSUE PLASMINOGEN ACTIVATOR (метаболизм)
ЛИПОПРОТЕИНЫ -- LIPOPROTEINS (метаболизм)
ГИПЕРТЕНЗИЯ -- HYPERTENSION (диагностика, осложнения)
Анотація: The present study was investigated of levels of oxidative modification of lipoproteins and content of plasminogen/plasmin system components – tissue-type plasminogen activator (t-PA) and plasminogen activators inhibitor-1 (PAI-1), in patients with stage II arterial hypertension (AHT) and resistant form of AHT. It was established that t-PA level in blood plasma of the patients is 2 times lower under stage II hypertension than normal and 2.5 times lower under resistant AHT. The inhibitor activity is 1.5 and 2 times higher consequently. It is concluded that patients with AHT have a decreased fibrinolytic potential, which can cause thrombotic states. Our evaluation showed a significant accumulation of products of lipid and protein oxidation, decrease of activity of antioxidant enzymes and changes of the activity of high density-lipoproteins-associated enzymes (decrease of paraoxonase-1 activity, increase of myeloperoxidase activity). Oxidized lipoproteins, t-PA and PAI-1 can be used as prognostic markers of development of complications and for evaluating the efficacy of therapy in patients with arterial hypertension
Дод.точки доступу:
Yusova, O. I.
Savchuk, O. V.
Grinenko, T. V.
Kuchmenko, O. B.
Mhitaryan, L. S.
Kupchins’ka, O. H.
Yevstratova, I. N.
Matova, O. O.
Vasilinchuk, N. M.
Drobot’ko, T. F.

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Salmanian Tabasi, N.
Designing, docking and heterologous expression of an anti-her2 affibody molecule / N. Salmanian Tabasi, A. Gholizadeh, B. Baghban Kohnehrouz // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 1. - P68-76

MeSH-головна:
НОВООБРАЗОВАНИЯ -- NEOPLASMS (генетика, диагностика, иммунология, терапия, химия)
МОЛЕКУЛЯРНОЙ СТЫКОВКИ МОДЕЛИРОВАНИЕ -- MOLECULAR DOCKING SIMULATION
ЭПИДЕРМАЛЬНЫЙ ФАКТОР РОСТА -- EPIDERMAL GROWTH FACTOR
БЕЛКИ -- PROTEINS (химический синтез)
Анотація: Affibody molecules are small protein scaffolds mostly based on triple-helical bundle protein domains. Many triple helix-based affibody proteins have shown prominent properties for tumor imaging and therapy. In our opinion, the structural organizations and the sizes of affibody molecules could be modified to increase their recognition abilities and binding affinities to human epidermal growth factor receptor type 2 (HER2). Thereby, the purpose of this study was to design and characterize a novel platform of affibody molecule consisting of five separate helixes (encoding 99 amino acids with a duplicate of helixes 1 and 2 at N-terminus plus GGGC chelator peptide sequence at C-terminus) enable of binding to HER2 with higher avidity. Using in silico screening methods, the structure and the interactive potential of designed affibody was comparatively investigated. The molecular expression and production of the designed affibody in Escherichia coli cells was successfully examined and reported
Дод.точки доступу:
Gholizadeh, A.
Baghban Kohnehrouz, B.

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Луговська, Г. Г.
Інноваційна діяльність інституту біохімії ім. О. В. Палладіна НАН України: проблеми і перспективи / Г. Г. Луговська, В. М. Данилова, С. В. Комісаренко // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 1. - С. 77-99

MeSH-головна:
АКАДЕМИИ И ИНСТИТУТЫ -- ACADEMIES AND INSTITUTES (история, организация и управление)
БИОХИМИЯ -- BIOCHEMISTRY (история, организация и управление)
ИЗОБРЕТЕНИЯ -- INVENTIONS (использование, история)
ИННОВАЦИЙ РАСПРОСТРАНЕНИЕ -- DIFFUSION OF INNOVATION
ПАТЕНТЫ -- PATENTS
ИНТЕЛЛЕКТУАЛЬНАЯ СОБСТВЕННОСТЬ -- INTELLECTUAL PROPERTY
Анотація: Проведено історико-наукознавчий аналіз досягнень науковців Інституту біохімії ім. О. В. Палладіна НАН України у винахідницькій діяльності для потреб медицини, сільського господарства, екології, промисловості. Проаналізовано об’єкти права інтелектуальної власності Інституту; описано перспективні інноваційні розробки. На основі проведеного аналізу зроблено висновок, що винахідницька діяльність наукових установ, зокрема Інституту біохімії ім. О. В. Палладіна НАН України, та впровадження наукових розробок, новітніх технологій у виробництво може забезпечити як підприємствам, так і країні в цілому економічне зростання та економію ресурсів, що є особливо важливим в умовах ринку
Дод.точки доступу:
Данилова, В. М.
Комісаренко, С. В.

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