Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Sandor, G. Vari
Knowledge sharing is the key for the progress of science / G. Vari Sandor // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 2. - P5-7

MeSH-головна:
БИОХИМИЯ -- BIOCHEMISTRY (обучение, организация и управление, тенденции)
ИННОВАЦИЙ РАСПРОСТРАНЕНИЕ -- DIFFUSION OF INNOVATION
Анотація: Scientists should share their raw data in ways that are easily accessible and digestible, and it is necessary that the published findings can be reanalyzed or replicated by others. Scientists need to publish the methods and findings more completely and science should be more transparent. Production of new knowledge in medicine is immeasurably facilitated by the enrichment of opportunities provided by efficient trans-national multidisciplinary scientific partnerships and collaborations like the RECOOP HST Association. The RECOOP HST Association enables the opportunity for development of diverse talents in different specialties, all geared towards sharing and integrating knowledge
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Simple two-step covalent protein conjugation to PEG-coated nanocrystals / S. Ya. Paryzhak [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 2. - P8-13

MeSH-головна:
НАНОЧАСТИЦЫ -- NANOPARTICLES
НАНОКОМПОЗИТЫ -- NANOCOMPOSITES
КВАНТОВЫЕ ТОЧКИ -- QUANTUM DOTS
Анотація: Covering of nanocrystals (NC) with a polyethylene glycol (PEG) envelop is a common way to increase their hydrophilicity, and compatibility with bio-systems, including increased retention time in the body. Colloidal semiconductor NC, also known as quantum dots (QD), particularly benefit from covering with PEG due to passivation of the inorganic core, while maintaining physical properties of the core. Despite many advantages of covering the surface with PEG, the covalent attachment of protein to hydroxyls of PEG is complicated. Here we propose a simple two-step approach for modification of PEG residues with subsequent covalent attachment of proteins. We were able to achieve specific NC targeting by means of attached protein as well as preserve their optical parameters (fluorescence intensity) in chemical reaction conditions. In the optimized protocol, ensuring removal of chemical byproducts by dialysis, we were able to omit the need for centrifugation (usually a limiting step due to particle size). The obtained NC-protein conjugate solutions contained 0.25x of initial unmodified NC amount, ensuring a low dilution of the sample. During all reactions the pH range was optimized to be between 6 to 8. The proposed approach can be easily modified for covalent targeting of different PEG-covered nanocomposites with proteins
Дод.точки доступу:
Paryzhak, S. Ya.
Dumych, T. I.
Karmash, O. I.
Bila, E. E.
Stachowiak, D.
Banski, M.
Podhorodecki, A.
Bilyy, R. O.

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Blood coagulation and aortic wall integrity in rats with obesity-induced insulin resistance / O. S. Dziuba [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 2. - P14-24

MeSH-головна:
ОЖИРЕНИЕ -- OBESITY
АТЕРОСКЛЕРОЗ -- ATHEROSCLEROSIS
ИНСУЛИНОРЕЗИСТЕНТНОСТЬ -- INSULIN RESISTANCE
ЭУКАРИОТЫ -- EUKARYOTA
Анотація: Obesity is an important factor in pathogenesis of disorders caused by chronic inflammation. Diet-induced obesity leads to dyslipidemia and insulin resistance (IR) that in turn provoke the development of type 2 diabetes and cardiovascular diseases. Thus, the aim of this work was to investigate the possible pro-atherogenic effects in the blood coagulation system and aortic wall of rats with obesity-induced IR. The experimental model was induced by a 6-month high-fat diet (HFD) in white rats. Blood samples were collected from 7 control and 14 obese IR rats. Prothrombin time (PT) and partial activated thromboplastin time (APTT) were performed by standard methods using Coagulometer Solar СТ 2410. Fibrinogen concentration in the blood plasma was determined by the modified spectrophotometric method. Levels of protein C (PC), prothrombin and factor X were measured using specific chromogenic substrates and activating enzymes from snake venoms. Platelet aggregation was measured and their count determined using Aggregometer Solar AP2110. The aorta samples were stained by hematoxylin and eosin according to Ehrlich. Aortic wall thickness was measured using morphometric program Image J. Statistical analysis was performed using Mann-Whitney U Test. The haemostasis system was characterized by estimation of the levels of individual coagulation factors, anticoagulant system involvement and platelet reactivity. PT and APTT demonstrated that blood coagulation time strongly tended to decrease in obese IR rats in comparison to the control group. It was also detected that 30% of studied obese IR rats had decreased factor X level, 40% had decreased level of prothrombin whereas fibrinogen concentration was slightly increased up to 3 mg/ml in 37% of obese IR rats. A prominent decrease of anticoagulant PC in blood plasma of obese rats was detected. Obese IR rats also had increased platelet count and higher rate of platelet aggregation in comparison to control animals. Histological analysis identified the disruption of aorta endothelium and tendency for the thickening of the aorta wall in the group with obesity-induced IR compared to the group of control rats. Changes of individual coagulation factors were assumed as the evidence of imbalance in the blood coagulation system. Increase of fibrinogen level, drop in PC concentration and pathological platelet reactivity were taken to corroborate the development of low-grade inflammation in obese IR rats. Instant generation of small amounts of thrombin in their blood plasma is expected. Since the aorta morphology assay detected the trend of its wall to thicken and the emergence of disruptions, we assumed there were initial stages of atherosclerosis and the danger of developing atherothrombosis. We detected an increase of blood coagulability and changes in aorta morphology in rats with obesity-induced IR which we assume indicate early development of atherosclerosis
Дод.точки доступу:
Dziuba, O. S.
Chernyshenko, V. O.
Hudz, Ie. A.
Kasatkina, L. O.
Chernyshenko, T. M.
Klymenko, P. P.
Kosiakova, H. V.
Platonova, T. M.
Hula, N. M.
Lugovskoy, E. V.

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Evaluation of antiproliferative activity of pyrazolothiazolopyrimidine derivatives / N. S. Finiuk [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 2. - P25-33

MeSH-головна:
ПИРИМИДИНЫ -- PYRIMIDINES (фармакология)
ДОКСОРУБИЦИН -- DOXORUBICIN (фармакология)
IN VITRO МЕТОДЫ -- IN VITRO TECHNIQUES
Анотація: The research aim was to test cytotoxic effects in vitro of seven novel pyrazolothiazolopyrimidine derivatives in targeting several lines of tumor and pseudo-normal mammalian cells. We demonstrated that cytotoxic effects of these derivatives depended on the tissue origin of targeted cells. Leukemia cells were found to be the most sensitive to the action of compounds 2 and 7. Compound 2 demonstrated approximately two times higher toxicity towards the multidrug-resistant sub-line of HL-60/ADR cells compared to the Doxorubicin effect. Antiproliferative action of compounds 2 and 7 dropped in the order: leukemia melanoma hepatocarcinoma glioblastoma colon carcinoma breast and ovarian carcinoma cells. These compounds were less toxic than Doxorubicin towards the non-tumor cells. The novel pyrazolothiazolopyrimidine, compound 2, demonstrated high toxicity towards human leukemia and, of special importance, towards multidrug-resistant leukemia cells, and low toxicity towards pseudo-normal cells
Дод.точки доступу:
Finiuk, N. S.
Ostapiuk, Yu. V.
Hreniukh, V. P.
Shalai, Ya. R.
Matiychuk, V. S.
Obushak, M. D.
Stoika, R. S.
Babsky, A. M.

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Biological and analytical studies of peritoneal dialysis solutions / N. Hudz [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 2. - P34-45

MeSH-головна:
ДИАЛИЗИРУЮЩИЕ РАСТВОРЫ -- DIALYSIS SOLUTIONS (фармакология)
Анотація: The purpose of our work was to conduct biological and analytical studies of the peritoneal dialysis (PD) solutions containing glucose and sodium lactate and establish correlations between cell viability of the Vero cell line and values of analytical indexes of the tested solutions. The results of this study confirm the cytotoxicity of the PD solutions even compared with the isotonic solution of sodium chloride, which may be due to the low pH of the solutions, presence of glucose degradation products (GDPs) and high osmolarity of the solutions, and unphysiological concentrations of glucose and sodium lactate. However, it is not yet known what factors or their combination and to what extent cause the cytotoxicity of PD solutions. In the neutral red (NR) test the weak, almost middle (r = -0.496 and 0.498, respectively) and unexpected correlations were found between reduced viability of monkey kidney cells and increased pH of the PD solutions and between increased cell viability and increased absorbance at 228 nm of the tested PD solutions. These two correlations can be explained by a strong correlation (r = -0.948) between a decrease in pH and an increase in the solution absorbance at 228 nm. The opposite effect was observed in the MTT test. The weak, but expected correlations (r = 0.32 and -0.202, respectively) were found between increased cell viability and increased pH in the PD solutions and between decreased cell viability and increased absorbance at 228 nm of the tested PD solutions. The middle and weak correlations (r = 0.56 and 0.29, respectively) were detected between increased cell viability and increased lactate concentration in the NR test and MTT test. The data of these correlations can be partially explained by the fact that a correlation with a coefficient r = -0.34 was found between decreased pH in the solutions and increased lactate concentration. The very weak correlations (0.138 and 0.196, respectively) were found between increased cell viability and increased glucose concentration in the NR test and MTT test. These experimental data indicate that pH is the dominating factor, which determines almost all of the established correlations. However, the character of the correlations is quite different: the higher the pH, the greater was the cell viability in the MTT test, and conversely, the higher the pH, the lower was the cell viability in the NR test. Secondly, the unexpected correlation coefficient was determined as -0.473 between decreased cell viability in the MTT test and increased cell viability in the NR test. Moreover, this phenomenon indicates that the mitochondrial enzyme succinate dehydrogenase is more vulnerable to the action of PD solutions than membrane permeability. Finally, we conclude that the NR test is not suitable for comparative studies of PD solutions which differ in pH, as it is pH dependent and does not enable the comparison of plausible cell viability
Дод.точки доступу:
Hudz, N.
Kobylinska, L.
Dmytrukha, N.
Korytniuk, R.
Wieczorek, P. P.

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Impact of N-acetylcysteine on antitumor activity of doxorubicin and landomycin A in NK/Ly lymphoma-bearing mice / Yu. S. Kozak [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 2. - P46-55

MeSH-головна:
АЦЕТИЛЦИСТЕИН -- ACETYLCYSTEINE (фармакология)
ДОКСОРУБИЦИН -- DOXORUBICIN (фармакология)
ЛИМФОМА -- LYMPHOMA
ЭУКАРИОТЫ -- EUKARYOTA
Анотація: N-acetylcysteine (NAC) is a dietary supplement demonstrating antioxidant and liver protecting effects that is widely used in clinics. NAC is considered to possess potential therapeutic activity for health disorders characterized by generation of free oxygen radicals, as well as potential for decreasing negative side effects of various drugs. However, the mechanisms of such tissue-protective actions of NAC remain poorly understood. The main aim of this work was to study therapeutic effects of NAC applied together with the “gold standard” of chemotherapy doxorubicin (Dx) or the novel experimental drug landomycin A (LA) to mice bearing NK/Ly lymphoma. It was revealed that NAC significantly decreased the nephrotoxicity of Dx (measured as creatinine level), possessed moderate immunomodulating activity (as revealed by an increase in number of cytotoxic T-lymphocytes), and partially increased survival of NK/Ly lymphoma-bearing animals treated with Dx. On the contrary, there was little tissue-protective effect of NAC towards LA due to the weak side effects of this anticancer drug, however, the combined use of NAC and LA significantly increased survival (60+ days) of LA-treated animals with NK/Ly lymphoma. Summarizing, NAC possesses a moderate tissue-protective activity towards Dx action but lacks a major therapeutic effect. However, in the case of LA action, NAC significantly increases its anticancer activity with no impact on its negative side effects. Further studies of the molecular mechanisms underlying that activity of NAC towards the action of LA are in progress
Дод.точки доступу:
Kozak, Yu. S.
Panchuk, R. R.
Skorokhyd, N. R.
Lehka, L. V.
Stoika, R. S.

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Osteoprotective effects of vitamin D(3) in diabetic mice is VDR-mediated and regulated via RANKL/RANK/OPG axis / D. O. Labudzynskyi [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 2. - P56-66

MeSH-головна:
ВИТАМИН D -- VITAMIN D (фармакология)
ДИАБЕТ САХАРНЫЙ ЭКСПЕРИМЕНТАЛЬНЫЙ -- DIABETES MELLITUS, EXPERIMENTAL
Анотація: There is growing evidence that vitamin D3 deficiency could be a contributing factor in the development of different chronic diseases and their complications. A better understanding of how diabetes influences bone tissue metabolism may become an underlying basis for effective prevention and treatment of skeletal disorders in diabetes. This study was performed to define diabetes-associated impairments in bone tissue remodeling in relation to vitamin D bioavailability and to estimate the effects of cholecalciferol treatment. We established that chronic hyperglycemia in diabetes was accompanied by a 2.15-fold decrease of 25OHD content in the serum. Vitamin D deficiency correlated with impairments of tibia biomechanical properties (decline of bone maximal load and stiffness values). µCT analysis of tibia showed respectively 3.0-, 2.1- and 1.3-fold decreases in trabecular bone volume per tissue volume, trabecular number and cortical thickness in diabetes indicating the development of secondary osteoporosis. Diabetes led to up-regulation of NF-κB/phosho-p65, RANKL, RANK (2.3-, 1.51-, 1.72-fold respectively) and down-regulation of OC, OPG and VDR (1.5-, 1.6- and 1.8-fold respectively) in tibial tissue of diabetic mice. Diabetes-associated abnormalities in the serum levels of RANKL, OPG and TRAP were also detected. Restoration of circulatory 25OHD content was achieved due to cholecalciferol treatment. Better vitamin D availability and increased VDR expression resulted in normalization of RANKL/RANK/OPG- and NF-κB-associated pathways that attenuated diabetes-induced structural and biomechanical abnormalities in bone tissue
Дод.точки доступу:
Labudzynskyi, D. O.
Shymanskyi, I. O.
Lisakovska, O. O.
Veliky, M. M.

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Clot formation and lysis in platelet rich plasma of healthy donors and patients with resistant hypertension / I. I. Patalakh [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 2. - P67-77

MeSH-головна:
ФИБРИНОВОГО СГУСТКА ЛИЗИСА ВРЕМЯ -- FIBRIN CLOT LYSIS TIME
ДОНОРЫ КРОВИ -- BLOOD DONORS
ГИПЕРТЕНЗИЯ -- HYPERTENSION
Анотація: Hemostatic balance in blood is affected by numerous factors, including coagulation and fibrinolytic proteins, the wide spectrum of their inhibitors, and blood cells. Since platelets can participate in contradictory processes, they significantly complicate the whole picture. Therefore, nowadays the development of global assays of hemostasis, which can reflect the physiological process of hemostasis and can be used for point-of-care diagnosis of thrombosis, is crucial. This paper outlines a new approach we used to analyze the capabilities of clot waveform analysis tools to distinguish the response of platelet-rich plasma from healthy donors and patients with arterial hypertension caused by stimulation of coagulation and lysis (with exogenous thrombin and recombinant tissue-type plasminogen activator, respectively). In donor plasma, when the clot degradation was accompanied by 40 IU/ml of recombinant tissue-type plasminogen activator, platelets potentiated fibrinolysis more than coagulation, which ultimately shifts the overall balance to a profibrinolytic state. At the same time, for patients with hypertension, platelets, embedded in clot obtained from platelet-rich plasma, showed a weaker ability to stimulate fibrinolysis. The obtained data gives the evidence that platelets can act not only as procoagulants but also as profibrinolytics. By simultaneously amplifying coagulation and fibrinolysis, making their rates comparable, platelets would control plasma procoagulant activity, thereby regulating local hemostatic balance, the size and lifetime of the clot. Moreover, clot waveform analysis may be used to distinguish the effects of platelet-rich plasma on clotting or lysis of fibrin clots in healthy donors and patients with essential hypertension.
Дод.точки доступу:
Patalakh, I. I.
Revka, O. V.
Kuchmenko, O. B.
Matova, O. O.
Drobotko, T. F.
Grinenko, T. V.

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