Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Загальна кількість знайдених документів : 15
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Biochemical aspects of the combined use of taxanes, irradiation and other antineoplastic agents for the treatment of anaplastic thyroid carcinoma [Text] / V. M. Pushkarev [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P5-18

MeSH-головна:
ЩИТОВИДНОЙ ЖЕЛЕЗЫ КАРЦИНОМА АНАПЛАСТИЧЕСКАЯ -- THYROID CARCINOMA, ANAPLASTIC (терапия)
Анотація: The review summarizes the results of the cycle of own research and literature data on biochemical mechanisms of combined action of taxanes with γ-irradiation and other antineoplastic agents on one of the most aggressive types of human cancer – anaplastic thyroid carcinoma. Antagonistic interplay between taxanes and irradiation at the level of apoptotic mechanisms and regulators of the cell cycle are discussed. The effectiveness and prospects of using low concentrations of taxanes and low doses of fractional γ-irradiation are substantiated. Attention is paid to the role of inflammation and its key factor – NF-κB in the genesis of thyroid carcinomas and their treatment. Directions for further research are outlined
Дод.точки доступу:
Pushkarev, V. M.
Kovzun, O. I.
Pushkarev, V. V.
Guda, B. B.
Tronko, M. D.

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Borzova, N. V.
Influence of chemical reagents and UV irradiation on the activity of Penicillium canescens α-galactosidase [Text] / N. V. Borzova, L. D. Varbanets // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P19-27

MeSH-головна:
PENICILLIUM -- PENICILLIUM (воздействие облучения, действие лекарственных препаратов)
Анотація: Investigations of the influence of chemical and physical factors on the conformational and functional properties of enzymes make a significant contribution to the study of the mechanism of action of industrially important proteins. The aim of the work was to evaluate the effect of chemical reagents and UV irradiation on the catalytic properties of Penicillium canescens α-galactosidase. Enzyme activity was assessed with p-nitrophenyl-α-D-galactopyranoside. Studies of the functionally active glycosidase groups were carried out on the basis of inhibitory and kinetic analysis using Dixon and Luinuiver-Burke methods with help of specific chemical reagents. A significant decrease in the activity of α-galactosidase in the presence of carbodiimides, diethylpyrocarbonate, the reagents on sulfhydryl groups was shown. A UV-induced decrease in enzyme activity in the dose range of 900-7200 J/m2 was noted. Based on the data obtained, the imidazole group of histidine, carboxyl groups of C-terminal amino acids and the SH-groups of cysteine are assumed to play an important role in the manifestation of the activity of P. canescens α-galactosidase
Дод.точки доступу:
Varbanets, L. D.

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Influence of organic solvents on the furin activity [Text] / T. V. Osadchuk [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P28-33

MeSH-головна:
ФУРИН -- FURIN (действие лекарственных препаратов)
Анотація: Furin belongs to a family of calcium-dependent serine proprotein convertases, which transform the inactive protein precursors into mature polypeptides. In model experiments, we studied the effect of organic solvents such as acetone, dimethyl sulfoxide (DMSO), dioxane, isopropanol and ethanol on the furin activity. Furin was found to retain up to 88% of its initial activity in the presence of DMSO, whereas in the presence of acetone only 30%. Organic solvents formed the following decreasing sequence of their effects on furin: acetone isopropanol ethanol dioxane dimethyl sulfoxide. The relationship between the residual furin activity and solvent parameters such as relative polarity, dipole moment and log P were investigated. The effect of the organic solvent appeared not to correlate with any of the listed characteristics. Laidler-Sсatchard’s graphs, which according to a theory must be linear, demostrated non-linearity. These results indicate that not only electrostatic interactions play an important role in the studied enzymatic reaction but also other factors, e.g. hydrophobic contacts, hydrogen bonds can influence furin catalysis. This seems relevant for further research in this area
Дод.точки доступу:
Osadchuk, T. V.
Shybyryn, O. V.
Semyroz, A. V.
Kibirev, V. K.

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Calix[4]arene C-956 selectively inhibits plasma membrane Ca2+,Mg2+-ATPase in myometrial cells [Text] / T. O. Veklich [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P34-42

MeSH-головна:
МИОМЕТРИЙ -- MYOMETRIUM (действие лекарственных препаратов, химия)
Анотація: Using enzymatic assays and kinetic analysis, we demonstrated that 100 µM calix[4]arene C-956 (5,11,17,23-tetra(trifluoro)methyl-(phenylsulfonylimino) methylamino-25,27-dioctyloxy-26,28-dipropoxycalix[4]arene) had the most significant inhibitory effect on the plasma membrane Са2+,Mg2+-АТРase activity compared to effects of other calix[4]arenes, and had no effect on specific activities of other membrane ATPases. Using confocal microscopy and fluorescent probe fluo-4, we observed an increase of the intracellular level of Ca2+ after application of calix[4]arene C-956 to immobilized myocytes. Analysis of the effect of calix[4]arene C-956 on the hydrodynamic diameter of myocytes demonstrated that application of calix[4]arene C-956 solution decreased this parameter by 45.5 ± 9.4% compared to control value similarly to the action of uterotonic drug oxytocin
Дод.точки доступу:
Veklich, T. O.
Skrabak, O. A.
Nikonishyna, Yu. V.
Rodik, R. V.
Kalchenko, V. I.
Kosterin, S. O.

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Afanasieva, K. S.
DNA loops after cell lysis resemble chromatin loops in an intact nucleus [Text] / K. S. Afanasieva, V. V. Olefirenko, A. V. Sivolob // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P43-49

MeSH-головна:
ФИБРИНОВОГО СГУСТКА ЛИЗИСА ВРЕМЯ -- FIBRIN CLOT LYSIS TIME (стандарты, тенденции)
ДНК -- DNA (действие лекарственных препаратов, физиология)
Анотація: The comet assay has proved itself to be not only a method of detection of DNA damages at the level of individual cells but also an approach for investigation of spatial organization of DNA loop domains in nucleoids. Usually, those nucleoids are obtained after cell lysis in high-salt buffer (e. g. 2.5 M NaCl) with a detergent: these conditions ensure the removal of cell membranes and most of the chromatin proteins, while supercoiled DNA loop domains remain untouched. In this work, we tested the comet assay applied to nucleoids obtained in low-salt solution (1 M NaCl). These nucleoids keep most of the histones and thus contain the loops resembling the chromatin loops to a greater extent. It was shown that, despite some quantitative differences, the most general features of the kinetics of DNA exit are about the same for nucleoids obtained in high- and low-salt conditions. It can be concluded that the DNA loops in high-salt nucleoids can be efficiently used to investigate the spatial DNA organization in chromatin
Дод.точки доступу:
Olefirenko, V. V.
Sivolob, A. V.

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Action of methyl jasmonate and salt stress on antioxidant system of Arabidopsis plants defective in jasmonate signaling genes [Text] / T. O. Yastreb [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P50-59

MeSH-головна:
КРЕСТОЦВЕТНЫЕ -- BRASSICACEAE (генетика, действие лекарственных препаратов)
Анотація: Role of jasmonate signaling in the regulation of stress-protective systems in Arabidopsis under salt stress remains insufficiently studied. For its clarification, comparative studies with mutants lacking various protein components of jasmonate signaling are advisable. In this connection, effects of methyl jasmonate (MJ, 50 μM) and salt stress (NaCl, 150 mM) on functioning of antioxidant and osmoprotective systems of wild-type Arabidopsis plants (Col-0) and ones defective in jasmonate signaling, namely coi1 (mutant for gene coding the protein COI1, which participates in removal of repressor proteins of transcription factors of jasmonate signaling) and jin1 (mutant defective in gene encoding the transcription factor JIN1/MYC2, one of the key in jasmonate signaling), were investigated. Salt stress inhibited growth of plants of all three genotypes. Treatment with MJ before salt stress positively influenced only the growth of wild-type plants. In contrast to mutants coi1 and jin1, Col-0 plants treated with MJ, under conditions of salt stress, kept close to the control values of water and total chlorophylls content, and the content of carotenoids increased. The coi1 plants under normal conditions differed from wild-type plants and jin1 mutants by reduced activity of guaiacol peroxidase and catalase and increased proline content. Treatment with MJ did not affect the activity of antioxidant enzymes and proline content in both mutants defective in jasmonate signaling. Under salt stress, the activity of superoxide dismutase, catalase and guaiacol peroxidase, as well as the content of proline and anthocyanins, in wild-type plants treated with MJ, were significantly higher than in control plants. The role of jasmonate-dependent protective systems in resistance of Arabidopsis plants to salt stress is discussed
Дод.точки доступу:
Yastreb, T. O.
Kolupaev, Yu. E.
Shvidenko, N. V.
Dmitriev, A. P.

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Overall hemostasis potential of the blood plasma and its relation to some molecular markers of the hemostasis system in patients with chronic renal disease of stage VD [Text] / B. G. Storozhuk [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P60-70

MeSH-головна:
ПОЧЕК БОЛЕЗНИ -- KIDNEY DISEASES (кровь)
Анотація: The values of the coagulation, overall and fibrinolysis potentials were estimated by the method of the global potential of Blomback M., as well as the values of concentrations of molecular markers of the hemostasis system: soluble fibrin (sf), D-dimer, fibrinogen (Fg) and protein C (88 patients, 52 of them men, 36 women). It was shown that hemostasis system activity in women plasma is higher than that in men plasma. The division of patients into 3 groups, depending on the concentration of sf: less than normal – sf ≤ 3, about norm – 3 sf 4 and more than norm – sf 4 μg/ml, allowed establishing the growth of the parameters of both the hemostatic potential and concentrations of molecular markers in accordance with concentration of sf in the groups of patients. Paerson’s correlation analysis of the relationship between the parameters of the hemostasis potential and concentrations of molecular markers revealed an increase in the correlation relationship to the strong and very strong between the parameters of coagulation, fibrinolysis and protein C systems with an increase in the concentration of soluble fibrin in plasma of patients
Дод.точки доступу:
Storozhuk, B. G.
Pyrogova, L. V.
Chernyshenko, T. M.
Kostiuchenko, O. P.
Kolesnikova, I. M.
Platonova, T. M.
Storozhuk, O. B.
Storozhuk, L. O.
Bereznitsky, G. K.
Tsap, P. Yu.
Masenko, O. O.
Makogonenko, E. M.
Lugovskoy, E. V.

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Evaluation of metallothioneins, oxidative stress and signs of cytotoxicity in young obese women [Text] / H. I. Falfushynska [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P71-80

MeSH-головна:
ОЖИРЕНИЕ ПАТОЛОГИЧЕСКОЕ -- OBESITY, MORBID (патофизиология)
Анотація: Obesity is rapidly increasing all over the world and pretends to be the global medical and social problem. Thus, the understanding of early signs of obesity and suitable biomarkers is urgently needed for developing an adequate strategy of the obesity prevention and a decrease in its growth rate. The parameters of the lipids’ metabolism and oxidative stress, metallothioneins and signs of cytotoxicity have been investigated in blood samples of young obese women (O-group, 32 Body Mass Index (BMI) 37). With regard to persons of O-group they had higher catalase activity (by 435%), level of reactive oxygen species (by 129%), level of oxidised glutathione (by 55%), lipid peroxidation (by 26%) and protein carbonyls (by 345%) in the blood, when compared with control. The obesity was accompanied by an increase in concentration of metallothioneins which have a partial tread effect on radical processes and reduce manifestations of oxidative damage to biomolecules in obese patients. The obese women had the signs of cytotoxicity as higher lactate dehydrogenase activity (by 387%) and DNA fragmentation (by 42%). The principal component analysis revealed the set of biological traits which describes the obesity progress and it included metallothioneins, parameters of oxidative stress, cytotoxicity, BMI and a concentration of low density lipoproteins and total cholesterol. The BMI was in a good correlation with parameters of the lipid metabolism, oxidative injury and cytotoxicity (r |0.73|, P 0.001)
Дод.точки доступу:
Falfushynska, H. I.
Horyn, O. I.
Khoma, V. V.
Tereshchuk, G. V.
Osadchuk, D. V.
Rusnak, N. I.
Stoliar, O. B.

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The effect of N-stearoylethanolamine on adipocytes free cholesterol content and phospholipid composition in rats with obesity-induced insulin resistance [Text] / O. S. Dziuba [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P81-90

MeSH-головна:
ОЖИРЕНИЕ -- OBESITY (осложнения)
ИНСУЛИНОРЕЗИСТЕНТНОСТЬ -- INSULIN RESISTANCE
Анотація: Obesity induces molecular changes that promote associated disorders, such as insulin resistance (IR) and type 2 diabetes. Low insulin sensitivity occurs primarily due to defects in the pathway of insulin action in target tissues, and there is a hypothesis that IR may originate in adipose tissue and is followed by dyslipidemia. In this study using methods of thin-layer and gas-liquid chromatography we investigated free cholesterol content and phospholipid composition of adipocytes of obesity-induced IR rats and its changes induced by the N-stearoylethanolamine (NSE) administration. The results we obtained demonstrated that free cholesterol content significantly increased in adipocytes of IR rats compared to control. The analysis of phospholipid composition indicated a reduction of phosphatidylcholine and the total content of phosphatidylinositol with phosphatidylserine, whereas the content of lysophosphatidylcholine, sphingomyelin and phosphatidylethanolamine increased in IR group compared to control. NSE administration caused a statistically significant decrease in total cholesterol level and had a considerable effect on normalization of individual phospholipids content. As far as NSE administration caused a statistically significant decrease in free cholesterol level and had a considerable effect on normalization of individual phospholipids content of adipocytes, we can consider NSE as a prospective compound worthy more complex investigation of its action under the pathological conditions
Дод.точки доступу:
Dziuba, O. S.
Hudz, Ie. A.
Kosiakova, H. V.
Horid’ko, T. M.
Klimashevsky, V. M.
Hula, N. M.

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10.

Krynytska, I. Ya.
The indices of nitrogen (II) oxide system in experimental hepatopulmonary syndrome [Text] / I. Ya. Krynytska, M. I. Marushchak // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P91-97

MeSH-головна:
ГЕПАТОПУЛЬМОНАЛЬНЫЙ СИНДРОМ -- HEPATOPULMONARY SYNDROME (патофизиология)
Анотація: Hepatopulmonary syndrome (HPS) is a pulmonary complication of liver disease characterized by arterial hypoxemia. Altered nitrogen (II) oxide (NO) production has also been implicated in the pathogenesis of the HPS. The present study was designed to evaluate the indices of NO system in the blood serum and lung tissue of animals with different models of hepatopulmonary syndrome. The total NOS activity was performed by monitoring the rate of conversion of L-arginine into citrulline. The total contents of NO metabolites was assessed by evaluation of their amount, which included nitrite ions that were previously presented in the sample (NO2–) and also nitrate ions reducted to nitrites (NO3–). We found a significant increase in total NOS activity in the lung tissue of Rats of both experimental groups as compared to control animals, but it was greater in the rats on the 28th day after the common bile duct ligation. The total concentration of NO2– + NO3– in the lung tissue of the rats in the experimental group N 1 also significantly increased (5.8 times) and in the rats of the experimental group with carbon – 4.5 times (P 0.001) vs the control group. Thus, in rats with different models of hepatopulmonary syndrome the activation of nitroxydergic process by a significant increase in nitrogen (II) oxide metabolites contents and total NO synthases activity has been established. Herewith a more pronounced intensification of nitroxydergic processes was observed in rats on the 28th day after the common bile duct ligation
Дод.точки доступу:
Marushchak, M. I.

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11.

Purification procedure and assay for the activity of lysyl oxidase [Text] / O. O. Gudkova [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P98-105

MeSH-головна:
ПРОТЕИН-ЛИЗИН-6-ОКСИДАЗА -- PROTEIN-LYSINE 6-OXIDASE (терапевтическое применение)
Анотація: The goal of the present study was to extract and purify lysyl oxidase from rodent’s tissues by a fast, simple, effective and inexpensive method and to develop a sensitive, time-saving lysyl oxidase specific activity assay for routine in vitro experiments. Lysyl oxidase was purified by elaborated purification procedure which relies on negative adsorption principle, that is, an effective decrease in the concentration of ballast components by the polar hydrophilic adsorbent and increasing the concentration of the protein of interest. Peroxide-coupled lysyl oxidase activity quantification methods based on luminol chemiluminescence in the presence of horseradish peroxidase as a catalyst and fluorescent detection using folic acid and Cu(II) with 1,5-diaminopentane as the substrate, were designed. Lysyl oxidase was partially purified from urea extracts of rodent’s tissues. Used purification procedure ensures the fast release of 93% of ballast proteins as shown by polyacrylamide gel electrophoresis. Lysyl oxidase specific activity after purification was 10-22-fold higher than that of the original extract. The molecular mass of murine lysyl oxidase from lung and heart was estimated to be ~32 kDa. We elaborated two sensitive methods for lysyl oxidase activity quantification and fast inexpensive procedure for partial enzyme purification useful in bulky in vitro experiments
Дод.точки доступу:
Gudkova, O. O.
Latyshko, N. V.
Zaitseva, O. V.
Shandrenko, S. G.

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12.

Kosterin, S. O.
Founder of molecular immunology in Ukraine, well-known political and public figure. On the 75th birthday of Academician of the NAS of Ukraine S. V. Komisarenko [Text] / S. O. Kosterin, V. M. Danilova // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P106-116

MeSH-головна:
АЛЛЕРГОЛОГИЯ И ИММУНОЛОГИЯ -- ALLERGY AND IMMUNOLOGY (история, кадры)
Анотація: July 9 marks the 75th birthday of Serhiy Vasylyovych Komisarenko – the well-known scientist-biochemist, founder of the scientific school in molecular immunology, diplomat, state and public figure, laureate of the State Prize of Ukraine in the Field of Science and Technology (1979), Honored Science and Technology Worker of Ukraine (2008), Winner of the O.V. Palladin Prize (2003) and I.I. Mechnikov Prize (2012) of the NAS of Ukraine, Academician-Secretary of the Division of Biochemistry, Physiology and Molecular Biology of the NAS of Ukraine, director of the O.V. Palladin Institute of Biochemistry of the NAS of Ukraine, Doctor of sciences (biology), Professor, Member of the NAS and NAMS of Ukraine.
Дод.точки доступу:
Danilova, V. M.

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13.

Matyshevska, O. P.
The FEBS3+ Meeting - XIth Parnas Conference - Young Scientists Forum ’Biochemistry and Molecular Biology for Innovative Medicine’ [Text] : 3-5 september 2018, Kyiv, Ukraine / O. P. Matyshevska // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P117-119

MeSH-головна:
БИОХИМИЯ -- BIOCHEMISTRY (тенденции)
МОЛЕКУЛЯРНАЯ БИОЛОГИЯ -- MOLECULAR BIOLOGY (тенденции)
МЕДИЦИНА -- MEDICINE (тенденции)
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14.

The Nobel Prize in Physiology or Medicine 2018 [Text] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P120

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15.

The Nobel Prize in Chemistry 2018 [Text] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P121

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© Міжнародна Асоціація користувачів і розробників електронних бібліотек і нових інформаційних технологій
(Асоціація ЕБНІТ)