Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Association of CD44⁺CD24^-/low with markers of aggressiveness and plasticity of cell lines and tumors of patients with breast cancer [Текст] / V. F. Chekhun [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 203-211. - Bibliogr. at the end of the art.

MeSH-главная:
МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS (осложнения, патофизиология, этиология)
НОВООБРАЗОВАНИЙ МАРКЕРЫ БИОЛОГИЧЕСКИЕ -- TUMOR MARKERS, BIOLOGICAL (анализ)
ЦИТОСТАТИЧЕСКИЕ СРЕДСТВА -- CYTOSTATIC AGENTS (анализ, терапевтическое применение)
ЛЕКАРСТВЕННАЯ УСТОЙЧИВОСТЬ НОВООБРАЗОВАНИЙ -- DRUG RESISTANCE, NEOPLASM (физиология)
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
Аннотация: To search for additional molecular-biological markers of cancer stem cell (CSC) involved in the development of intra-tumor heterogeneity for the detection of features of the breast cancer (BC) pathogenesis. Materials and methods: Expression of estrogen receptors (ER), progesterone receptors (PR), Her2/neu, E- and N-cadherin, CD24, CD44, Bcl-2, Bax, Slug, P-gp, glutathione-S-transferase (GST) and metallothionein in cell lines was determined by the immunocytochemical method. Expression of ER, PR, Her2/neu, CD24 and CD44 in the surgical material of BC patients were determined by the immunohistochemical method. The levels of the miRNA were determined using real-time polymerase chain reaction. Results: Cells of high-grade malignancy (HGM), MDA-MB-231 and MDA-MB-468 are characterized by high expression of stem cell markers compared to the cells of low-grade malignancy (LGM), T47D and MCF-7: CD44 levels in T47D and MCF-7 cells were in range of 72-79 points, which is significantly lower than in HGM cells (p 0.05). Also, HGM cells with the properties of CSC were characterized by high expression of antiapoptotic proteins, the transcription factor Slug, and low levels of proapoptotic protein Bax (p 0.05) compared to LGM cells. In cells with CSC characteristics an increased expression of transferrin and its receptor, ferritin, fentorin and hepcidin was revealed indicating activation of the endogenous iron metabolism. The characteristic feature of HGM cells with CSC phenotype were the increased levels of oncogenic miR-221, -155 and -10b by 60%, 92% and 78%, respectively, and decreased levels of oncosuppressive miR-29b, -34a and -200b by 8.4 ± 0.3, 4.6 ± 0.2, and 3.4 ± 0.6 times compared to MCF-7 line cells. It has been established that the development of resistance to cytostatics is accompanied by increased aggressiveness of tumor cells, loss of expression of hormonal receptors and acquiring of stem phenotype. In particular, increased expression of P-gp was observed in BC cells during the development of resistance to doxorubicin, of GST during the development of resistance to cisplatin along with increased CD44 expression (p 0.05). We have revealed the relation between the presence of cells with the CSC phenotype (CD44+CD24-/low) and clinical and pathological characteristics of BC patients, their survival and BC sensitivity to neoadjuvant therapy (p > 0.05). Conclusions: The dependence between the expression of CSC markers and the degree of malignancy of tumor cells, development of resistance to cytostatics in vitro was established as well as the predictive value of the detection of the CSC for the individual prognosis of the BC course and sensitivity of the tumors to the treatment
Доп.точки доступа:
Chekhun, V. F.
Lukianova, N. Y.
Chekhun, S. V.
Bezdieniezhnykh, N. O.
Zadvomiy, T. V.
Borikun, T. V.
Polishchuk, L. Z.
Klyusov, O. M.

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Bezdieniezhnykh, N. O.
Scientific-practical and legal problems of implementation of the personalized medicine [Текст] / N. O. Bezdieniezhnykh, V. V. Reznikova, O. V. Rossylna // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 229-233

MeSH-главная:
ЗДОРОВЬЯ ИНДИВИДУАЛЬНОГО ОХРАНА -- PERSONAL HEALTH SERVICES (законодательство и юриспруденция, методы, организация и управление, тенденции)
МЕДИЦИНСКОЙ ПОМОЩИ ПЛАНИРОВАНИЕ ИНДИВИДУАЛЬНОЕ -- ADVANCE CARE PLANNING (законодательство и юриспруденция, использование, организация и управление, статистика, тенденции)
МЕЖДУНАРОДНОЕ СОТРУДНИЧЕСТВО -- INTERNATIONAL COOPERATION (законодательство и юриспруденция)
ЛЕЧЕБНО-ДИАГНОСТИЧЕСКАЯ ТАКТИКА -- CRITICAL PATHWAYS (законодательство и юриспруденция, организация и управление, тенденции)
Аннотация: The article is devoted to the comprehensive analysis of scientific, practical and legal issues of personalized medicine that is a rapidly developing science-driven approach to healthcare. It is concluded that there is lack of general legal framework for the encouragement of scientific researches and practical implementation in this field. The article shows foreign experience and prospects for the introduction of personalized medicine as a key concept of healthcare system, which is based on a selection of diagnostic, therapeutic and preventive measures that would be the most effective for a particular person in view of individual characteristics. The conclusions and proposals to improve the current legislation and development of personalized medicine in Ukraine are suggested
Доп.точки доступа:
Reznikova, V. V.
Rossylna, O. V.

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Шифр: ЕУ12/2017/39/3
Журнал

Экспериментальная онкология [Текст]
2017г. Т. 39 № 3 . - 34.76, р.

Содержание:
Chekhun, V. F. On the 40th anniversary of the international symposium “The role of stem cells in leukemo-and carcinogenesis»: in Kyiv again / V. F. Chekhun. - С.162-163
Kalynychenko, T. O. Umbilical cord blood banking in the worldwide hematopoietic stem cell transplantation system: perspectives for Ukraine / T. O. Kalynychenko. - С.164-170. - Bibliogr. at the end of the art.
Vasyliev, R. G. Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications / R. G. Vasyliev [та ін.]. - С.171-180. - Bibliogr. at the end of the art.
Другие авторы: Rodnichenko A. E., Gubar O. S., Zlatska A. V., Gordiienko I. M., Novikova S. N., Zubov D. O.
Kozub, M. M. Comparison of various tissue and cell therapy approaches when restoring ovarian, hepatic and kidney’s function after chemotherapy-induced ovarian failure / M. M. Kozub [та ін.]. - С.181-185. - Bibliogr. at the end of the art.
Другие авторы: Prokopiuk V. Y., Skibina K. R., Prokopiuk О. V., Kozub N. I.
Gubar, O. S. Postnatal extra-embryonic tissues as a source of multiple cell types for regenerative medicine applications / O. S. Gubar [та ін.]. - С.186-190. - Bibliogr. at the end of the art.
Другие авторы: Rodnichenko A. E., Vasyliev R. G., Zlatska A. V., Zubov D. O.
Vasyliev, R. G. Tissue-engineered bone for treatment of combat related limb injuries / R. G. Vasyliev [та ін.]. - С.191-196. - Bibliogr. at the end of the art.
Другие авторы: Oksymets V. M., Rodnichenko A. E., Zlatska A. V., Gubar O. S., Gordiienko I. M., Zubov D. O.
Zlatska, A. V. Endometrial stromal cells: isolation, expansion, morphological and functional properties / A. V. Zlatska [та ін.]. - С.197-202. - Bibliogr. at the end of the art.
Другие авторы: Rodnichenko A. E., Gubar O. S., Zubov D. O., Novikova S. N., Vasyliev R. G.
Chekhun, V. F. Association of CD44⁺CD24^-/low with markers of aggressiveness and plasticity of cell lines and tumors of patients with breast cancer / V. F. Chekhun [та ін.]. - С.203-211. - Bibliogr. at the end of the art.
Другие авторы: Lukianova N. Y., Chekhun S. V., Bezdieniezhnykh N. O., Zadvomiy T. V., Borikun T. V., Polishchuk L. Z., Klyusov O. M.
Karlitepe, A. Anti-cancer efficiency of natural killer cells differentiated from human adipose tissue-derived mesenchymal stem cells and transfected with miRNA150 / A. Karlitepe [та ін.]. - С.212-218. - Bibliogr. at the end of the art.
Другие авторы: Kabadayi H., Vatansever S., Gurdal M., Gunduz C., Ercan G.
Lisyaniy, N. I. Content of stem tumor CD133⁺ cells in brain neoplasms of different histological type / N. I. Lisyaniy [та ін.]. - С.219-223. - Bibliogr. at the end of the art.
Другие авторы: Stanetskaya D. N., Lisyaniy A. N., Belskaya L. N.
Ryspayeva, D. E. Are CD44⁺CD24^- cells the assumed cancer stem cells in breast cancer? / D. E. Ryspayeva [та ін.]. - С.224-228. - Bibliogr. at the end of the art.
Другие авторы: Smolanka I. I., Dudnichenko A. S., Lyashenko A. A., Grinevich Yu. A., Gurianov V. G., Koshubarova M. V., Seleznev A. A.
Bezdieniezhnykh, N. O. Scientific-practical and legal problems of implementation of the personalized medicine / N. O. Bezdieniezhnykh, V. V. Reznikova, O. V. Rossylna. - С.229-233
Normal and cancer stem cells: discovery diagnosis and therapy international scientific conference. R. E. Kavetsky Institute of Experimental Pathology Oncology and Radiobiology National Academy of Sciences of Ukraine Kyiv October 5–6 2017. - С.234-256
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