Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Заглавие журнала :The Ukrainian biochemical journal -2021г. т.93,N 2
Интересные статьи :
Vari S. G. Inflammation is the common mechanism of diseases (CMD) in COVID-19 disease during pregnancy and in gestational diabetes mellitus/ S. G. Vari (стр.5)
Kobylinska L. In silico identification and biochemical validation of plausible molecular targets of 4-thiazolidinone derivative les-3833 as a potential anticancer agent/ L. Kobylinska [et al.] (стр.7-23)
Shevchuk O. O. Locally delivered lovastatin-containing chitosan nanoparticles promote bone regeneration in rats/ O. O. Shevchuk, Ya. V. Panasiuk, M. M. Korda (стр.24-38)
Finiuk N. S. Effect of a novel thiazole derivative and its complex with a polymeric carrier on stability of DNA in human breast cancer cells/ N. S. Finiuk [et al.] (стр.39-52)
Poshyvak O. B. Oxidative stress suppression contributes to antiseizure action of axitinib and rapamycin in pentylenetetrazol-induced kindling/ O. B. Poshyvak, O. R. Pinyazhko, L. S. Godlevsky (стр.53-60)
Korolova D. S. Thromboelastographic study of fibrin clot and molecular basis of maximum clot firmness/ D. S. Korolova [et al.] (стр.62-70)
Pavlyshyn H. A. The association between vitamin D status and cardiometabolic disorders in overweight and obese adolescents/ H. A. Pavlyshyn, A.-M. A. Shulha (стр.72-81)
Pavlyshyn H. A. Investigating the connections between night eating syndrome and metabolic syndrome in children/ H. A. Pavlyshyn, K. V. Kozak, K. T. Hlushko (стр.83-94)
Hlushko K. T. Clinical and immunological peculiarities of parasitic infections in children with digestive system disorders in Western Ukraine/ K. T. Hlushko, H. A. Pavlyshyn, K. V. Kozak (стр.96-105)
Kucher S. V. Inclusion of enterosorbents in anti-inflammatory therapy improve treatment effectiveness in COPD patients during exacerbations/ S. V. Kucher, O. V. Lototska (стр.107-114)
Danylova T. V. Double Nobel prize winner: Frederick Sanger - the father of genomics/ T. V. Danylova, S. V. Komisarenko (стр.116-122)
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Korolova D. S., Stohnii Y. M., Gryshchuk V. I., Zhuk S. I., Us I. V., Chernyshenko T. M., Kostiuchenko O. P., Klymenko K. P., Platonov O. M., Ivashchenko O. I., Chernyshenko V. O.
Заглавие : Thromboelastographic study of fibrin clot and molecular basis of maximum clot firmness
Место публикации : The Ukrainian biochemical journal. - Київ, 2021. - Vol. 93, № 2. - С. 62-70 (Шифр УУ60/2021/93/2)
MeSH-главная: ФИБРИН -- FIBRIN
ТРОМБОЭЛАСТОГРАФИЯ -- THROMBELASTOGRAPHY
Аннотация: Maximum clot firmness (MCF) is the main parameter of thromboelastography (TEG) reflecting the stability of a clot. In this work, we looked for markers that can influence the enhancement of MCF and detected molecular markers and blood clotting parameters that can be involved in such mechanisms. Blood samples of pregnant women with placental disorders were collected in the Kyiv Perinatal Center. TEG was performed on whole blood in EXTEM and INTEM tests. APTT, INR, fibrinogen concentration and platelet aggregation were measured using traditional laboratory approaches. D-dimer was detected in sandwich ELISA using monoclonal antibodies III-3B and II-4D. The relative cross-linking activity of factor XIIIa was measured by the direct quantification of the cross-linked γ-chain of fibrin using Western-Blotting with monoclonal antibody II-4D. D-dimer and fibrinogen concentrations, clotting time in the APTT test, INR and rate of platelet aggregation did not correlate with the MCF. However, we found positive correlations of MCF with factor XIIIa activity: 0.51 and 0.87 for EXTEM and INTEM, respectively. These data indicate that for normal and slightly increased fibrinogen concentrations, fibrin clot firmness will depend mostly on the activity of factor XIIIa. Thus the direct determination of factor XIIIa activity in blood plasma of patients can be relevant for predicting the risk of intravascular coagulation. Evaluation of the content and activity of individual clotting factors or other components of the coagulation system can be useful additions to the TEG diagnostics and should not be neglected
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Stohnii Y. M., Ryzhykova M. V., Rebriev A. V., Kuchma M. D., Marunych R. Y., Chernyshenko V. O., Shablii V. A., Lypova N. M., Slominskyi O. Yu., Garmanchuk L. V., Platonova T. M., Komisarenko S. V.
Заглавие : Aggregation of platelets, proliferation of endothelial cells and motility of cancer cells are mediated by the Bβ1(15)-42 residue of fibrin(ogen)
Место публикации : The Ukrainian biochemical journal. - Київ, 2020. - Vol. 92, № 2. - С. 72-85 (Шифр УУ60/2020/92/2)
MeSH-главная: ФИБРИНОГЕН -- FIBRINOGEN
НОВООБРАЗОВАНИЙ ИНВАЗИВНОСТЬ -- NEOPLASM INVASIVENESS
Аннотация: The fibrinogen molecule contains multiple binding motifs for different types of cellular receptors, acting as a molecular link between coagulation and cell adhesion. In this study we generated a truncated form of the fibrinogen molecule lacking the Bβ1-42 sequence by site-specific proteolysis and evaluated the role of the fragment in adhesive capabilities of platelets, endothelial and cancer cells. Fibrinogen with the removed Bβ1-42 sequence and fibrin without the Bβ15-42 fragment (desβ1-42 fibrinogen and desABβ15-42 fibrin) were obtained by proteolysis using the specific protease from the venom of Echis multisquamatis. The cleaved fragment was purified by HPLC and was identified using MALDI-TOF. ADP- and collagen-induced aggregation of washed platelets in the presence of fibrinogen desBβ1-42 was studied using an aggregometer. Proliferation of mice aortic endothelial cells (MAEC) and human umbilical vein endothelial cells (HUVEC) was studied using the fibrin desABβ15-42 as the scaffold. Cell viability was quantified by the MTT test (MAEC). Generation time was calculated for the estimation of proliferative activity of HUVEC. Lung cancer cell line Н1299 was used to evaluate cancer cell motility in vitro using the scratch assay. Direct comparison of cellular behavior in the presence of truncated vs native forms demonstrated attenuated cell adhesion in the presence of fibrinogen desBβ1-42 and fibrin desBβ15-42. The platelet aggregation rate was only slightly decreased in the presence of fibrinogen desBβ1-42 but resulted in 15-20% disaggregation of adhered platelets. We also observed the substantial decrease of generation time of HUVEC and inhibition of viability of MAEC cells grown on scaffolds of a desABβ15-42 matrix. Finally, desBβ1-42 modulated the motility of H1299 cells in vitro and suppressed the wound healing by 20% compared to the full-length fibrinogen. We postulate that fragment 1-42 of the BβN-domain of fibrinogen is not sufficient for platelet aggregation, however it may contribute to platelet clot formation in later stages. At the same time, this fragment may be important for establishing proper cell-to-cell contacts and cell viability of endothelial cells. Also, 1-42 amino acid fragment of the BβN-domain supported the migration of cancer cells suggesting that interactions of fibrinogen with cancer cells could be a target for anticancer therapy. The Bβ1-42 fragment of fibrinogen contributes to efficient intracellular interactions of different types of cells, including platelets, endothelial cells and cancer cells
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tkachenko O. S., Hudz Ie. A., Kosiakova H. V., Klymenko P. P., Stohnii Y. M., Didkivskyi V. A., Chernyshenko T. M., Chernyshenko V. O., Platonova T. M.
Заглавие : Protective action of N-stearoylethanolamine on blood coagulation and arterial changes in spontaneously hypertensive rats fed cholesterol-rich diet
Место публикации : The Ukrainian biochemical journal. - Київ, 2020. - Vol. 92, № 2. - С. 60-71 (Шифр УУ60/2020/92/2)
MeSH-главная: ГИПЕРТЕНЗИЯ -- HYPERTENSION
ХОЛЕСТЕРИН -- CHOLESTEROL
ЭУКАРИОТЫ -- EUKARYOTA
Аннотация: In this work we aimed to test the atherosclerotic changes in the aortic wall and pro-coagulant response of the blood coagulation system of spontaneously hypertensive rats (SHR) fed cholesterol-rich diet (CRD) and to study the effect of the anti-inflammatory agent N-stearoylethanolamine (NSE) on the development of atherosclerosis in this model. Female rats (n = 30) with genetically determined hypertension proven by direct measurement of blood pressure were fed CRD (5% cholesterol) for 2 months. Control group of SHR (n = 10) received standard pellet diet, 10 were fed CRD and 10 received CRD with daily per os application of NSE at a dose of 50 mg/kg of body weight. Histological analysis detected swelling and detachment of endothelial cells, huge edema of the subendothelial layer and a disruption of the middle shell integrity. CRD rats had higher fibrinogen concentration, increased rate of platelet aggregation and decreased level of anticoagulant PC. Platelet aggregation speed increased in CRD-fed rats (52.5±4.1%/min) was slightly normalized under the action of NSE (40±8.3 vs 35±9%/min in controls). Fibrinogen concentration was slightly increased in CRD-fed rats (2.75±0.7 vs 1.9±0.5 mg/ml in controls). However, the level of anticoagulant PC that was decreased in CRD-fed rats (65±16 vs 100±11% in controls) was normalized under the action of NSE (92±17%). NSE also influenced the aorta architecture, however normalizing the thickness of the aorticwall did not change the cholesterol-induced inclusions within aorta media. NSE anti-inflammatory action changes the atherogenic processes in CRD-fed rats mainly protecting PC from consumption during the inflammatory process and reducing edema of the aorta. However hematological parameters (including clotting time in the APTT test and fibrinogen concentration) changed independently on NSE application. Anti-aggregatory action of NSE on platelets can be a result of direct action on platelets or the consequence of its anti-inflammatory action. During atherogenesis induced by CRD in the model, NSE demonstrated valuable anti-inflammatory action protecting the organism during atherogenesis, however it cannot be assumed as an antithrombotic or antiatherogenic agent because it is unable to influence hemostasis directly
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Заглавие журнала :The Ukrainian biochemical journal -2020г. т.92,N 2
Интересные статьи :
Vari S. G. Homo scientist can lead HOMO SAPIENS to the next evolutionary stage, to HOMO ingenious/ S. G. Vari (стр.5-6)
Korolyuk O. Ya. Hypertriclyceridemia is associated with long-term risk of cardiovascular events and specific comorbidity in very high-risk hypertensive patients/ O. Ya. Korolyuk, O. M. Radchenko (стр.8-19)
Bozhenko M. Freezing influences, the exposure of IgG glycans in sera from multiple sclerosis patients/ M. Bozhenko [et al.] (стр.21-31)
Nasadyuk C. M. Lectinocytochemical study of rat stomach mucosa under the conditions of cyclooxygenase-1/-2 blockage and pretreatment with H-GLU-ASP-GLY-OH/ C. M. Nasadyuk [et al.] (стр.33-43)
Onopchenko O. V. The effect of N-stearoylethanolamine on the lipid composition of the rat testes and testosterone level during the early stages of streptozotocin-induced diabetes/ O. V. Onopchenko [et al.] (стр.45-59)
Tkachenko O. S. Protective action of N-stearoylethanolamine on blood coagulation and arterial changes in spontaneously hypertensive rats fed cholesterol-rich diet/ O. S. Tkachenko [et al.] (стр.60-71)
Stohnii Y. M. Aggregation of platelets, proliferation of endothelial cells and motility of cancer cells are mediated by the Bβ1(15)-42 residue of fibrin(ogen)/ Y. M. Stohnii [et al.] (стр.72-85)
Revenko O. Exogenous hydrogen sulfide for the treatment of mesenteric damage associated with fructose-induced malfunctions via inhibition of oxidative stress/ O. Revenko [et al.] (стр.86-98)
Bila G. Novel approach for discrimination of eosinophilic granulocytes and evaluation of their surface receptors in a multicolor fluorescent histological assessment/ G. Bila [et al.] (стр.99-107)
Kryshchyshyn A. Synthesis and anti-leukemic activity of pyrrolidinedione-thiazolidinone hybrids/ A. Kryshchyshyn [et al.] (стр.108-120)
Hreniukh V. P. Effects of thiazole derivatives on intracellular structure and functions in murine lymphoma cells/ V. P. Hreniukh [et al.] (стр.121-131)
Chaban T. I. Development of effective anti-inflammatory drug candidates among novel thiazolopyridines/ T. I. Chaban [et al.] (стр.132-139)
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chernyshenko V. O.
Заглавие : Men of the molecules
Место публикации : The Ukrainian biochemical journal. - Київ, 2020. - Vol. 92, № 3. - С. 86-90 (Шифр УУ60/2020/92/3)
MeSH-главная: БИОХИМИЯ -- BIOCHEMISTRY
Аннотация: In memoriam Eduard Lugovskoi and Russell Doolittle we are referring to several episodes of their life and work. Russell Doolittle an American biochemist and his friend and colleague Ukrainian scientist Eduard Lugovskoi, both studied fibrinogen structure and functions and finally united their efforts in the revealing of the new mechanism of intramolecular interactions of fibrin molecule through coiled-coil region. The results of their common work and discussions were included to the article “The fibrin Bβ125-135 site is involved in the lateral association of protofibrils”. Valuable part of the communication dedicated to the poetry of Eduard Lugovskoi that inspired both of scientists in work and life. We are providing some remembrance of their collaboration, their letters sent to each other, fragments of handwriting and common photo of Russell Doolittle and Eduard Lugovskoi
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lugovska N. E., Kolesnikova I. M., Stohnii Ye. M., Chernyshenko V. O., Rebriev A. V., Kostiuchenko O. P., Gogolinska G. K., Dziubliuk N. A., Varbanets L. D., Platonova T. M., Komisarenko S. V.
Заглавие : Novel monoclonal antibody to fibrin(ogen) αC-region for detection of the earliest forms of soluble fibrin
Место публикации : The Ukrainian biochemical journal. - Київ, 2020. - Vol. 92, № 3. - С. 58-70 (Шифр УУ60/2020/92/3)
MeSH-главная: ФИБРИН -- FIBRIN
Аннотация: Obtaining new monoclonal antibodies (mAbs) towards fibrin(ogen) and its fragments is an important task for studying mechanisms of blood clot formation, searching for novel antithrombotic agents and developing immunodiagnostics. The aim of the present work was to create and characterize a new mAb towards the fibrin(ogen) αС-region. We surmise that having a specific mAb towards this flexible part of the molecule will allow us to study the role of the αС-region in fibrin polymerization and also to develop an approach for detecting the earliest forms of soluble fibrin by sandwich ELISA. Using hybridoma technology we оbtained mAb 1-5A to the αC-region of fibrinogen. It was characterized using several variations of ELISA and Western blot. Application of specific proteases together with MALDI-TOF analysis allowed us to localize its epitope that is located in fragment 537-595 of the Aα-chain of fibrin(ogen). МAb 1-5A can be used as a detecting tag-antibody in sandwich ELISA for the quantification of the earliest forms of soluble fibrin which are uncleaved by plasmin and preserved C-terminal portions of αC-regions. These earliest forms of soluble fibrin are direct evidence of blood coagulation system activation, thrombin generation and the danger of intravascular thrombus formation. Their determination will provide additional, more accurate information about the state of the blood coagulation system and the risk of blood clotting, which is very important for the timely and correct selection of adequate antithrombotic therapy. MAb 1-5A effectively binds the αC-containing molecules of fibrinogen and fibrin in blood plasma. It also can be used for studying protein-protein and protein-cellular interactions of the αC-regions of fibrin(ogen)
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Заглавие журнала :The Ukrainian biochemical journal -2020г. т.92,N 3
Интересные статьи :
Chernyshenko V. Presenting thematic ’Molecular and clinical studies of hemostasis’ issue/ V. Chernyshenko, S. Komisarenko (стр.5)
Vari S. G. COVID-19 infection: disease mechanism, vascular dysfunction, immune responses, markers, multiorgan failure, treatments, and vaccination/ S. G. Vari
Medved L. Structure and function of fibrinogen BβN-Domains/ L. Medved, S. Yakovlev (стр.22-32)
Lugovskoi E. The fibrin Bβ125-135 site is involved in the lateral association of protofibrils/ E. Lugovskoi [et al.] (стр.33-45)
Kapustianenko L. G. Identification of the binding site for plasminogen kringle 5 in the α-chain of fibrin(ogen) D-fragment/ L. G. Kapustianenko [et al.] (стр.46-57)
Lugovska N. E. Novel monoclonal antibody to fibrin(ogen) αC-region for detection of the earliest forms of soluble fibrin/ N. E. Lugovska [et al.] (стр.58-70)
Mykhaloiko I. S. D-dimer as a potential predictor of thromboembolic and cardiovascular complications in patients with chronic kidney disease/ I. S. Mykhaloiko [et al.] (стр.71-76)
Panibratiuk O. A. Warfarin therapy in patients with coronary heart disease and atrial fibrillation: drug interactions and genetic sensitivity to warfarin/ O. A. Panibratiuk, O. A. Yakovleva (стр.77-85)
Chernyshenko V. O. Men of the molecules/ V. O. Chernyshenko (стр.86-90)
Григор’єва М. В. 20 років тому завершилися "перегони" секвенування геному людини/ М. В. Григор’єва ; М. В. Григор’єва, С. В. Комісаренко (стр.91-92)
Danylova T. V. Nobel prize winner erwin schrodinger: the physicist, philosopher, and godfather of molecular biology and genetics/ T. V. Danylova, S. V. Komisarenko (стр.93-100)
До 70-річчя від дня народження члена-кореспондента НАН України, професора Ростислава Стефановича Стойки (стр.101-102)
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gryshchenko V. A., Musiychuk V. V., Chernyshenko V. O., Gornytska O. V., Platonova T. M.
Заглавие : Evaluation of biochemical indicators in blood plasma of rats with tetracycline-induced hepatosis and their correction by milk phospholipids
Параллельн. заглавия :Оцінка біохімічних показників плазми крові щурів за тетрациклін¬індукованого гепатозу та їх коригування фосфоліпідами молока
Место публикации : The Ukrainian Biochemical Journal. - 2019. - Т. 91, № 1. - С. 92-99 (Шифр УУ60/2019/91/1)
Примечания : Bibliogr. at the end of the art.
MeSH-главная: БОЛЕЗНЬ, МОДЕЛИ НА ЖИВОТНЫХ -- DISEASE MODELS, ANIMAL
ТЕТРАЦИКЛИН -- TETRACYCLINE
ПЕЧЕНИ БОЛЕЗНИ -- LIVER DISEASES
ФОСФОЛИПИДЫ -- PHOSPHOLIPIDS
МОЛОКА БЕЛКИ -- MILK PROTEINS
Аннотация: Tetracycline is a drug with direct cytotoxic action on the liver, and therefore it is widely used in pharmaceutical studies of therapeutic effectiveness of hepatoprotective preparations. The aim of the present work was to determine the biochemical indicators in blood plasma of rats with tetracycline-induced hepatosis and correction properties of milk phospholipids under tetracycline-induced hepatosis in rats. To achieve this, Wistar rats were administered 250 mg/kg of 4% tetracycline hydrochloride suspension once a day intragastrically. As the corrective therapy, 1% solution of BAS “FLP-MD” was administered in liposomal form based on milk phospholipids. Under modeled steatohepatitis, significant destructive changes were observed in the cell membranes of hepatocytes in experimental rats. It was confirmed by higher activity of transaminase (in particular, activity of АSТ increased 4 times, that of ALT 1.7 times and the AST/ALT ratio was increased 2.4 times in blood plasma). The synthesis of clotting factors in livers of animals with hepatosis was inhibited. The content of fibrinogen in blood plasma decreased by 21%, factor II (prothrombin) by 27.8%, Xa-factor by 27.9%, and protein C by 40.6%. The animals also had hypochromic anemia, azotemia and bilirubinemia. The calcium-phosphor metabolism and hyperkalemia were observed. The liposomal BAS “FLP-MD” based on milk phospholipids diminished harmful effects of tetracycline, in particular supporting blood coagulation factors’ level restoration, and also by the activity of transaminases. According to the results, it may be used in prophylactics and pharmaceutical correction of steatohepatitisТетрациклін виявляє виражений цитотоксичний ефект на печінку, відтак його широко використовують для тестування терапевтичної ефективності гепатопротекторних препаратів. Метою роботи було визначення біохімічних показників плазми крові щурів та коригувальних властивостей фосфоліпідів молока за тетрациклініндукованого гепатозу. Для моделювання гепатозу щурам щоденно вводили 4%-й розчин тетрацикліну гідрохлориду в дозі 250 мг/кг маси тіла внутрішньошлунково. Корекційну терапію проводили за допомогою 1%-ї суспензії фосфоліпідів молока у формі ліпосомальної біологічно активної добавки «FLP-MD». Експериментальний гепатоз зумовлював вірогідні деструктивні зміни в мембранах гепатоцитів. Це було підтверджено підвищеною амінотрансферазною активністю (зокрема, в плазмі крові активність АСТ зростала у 4,0 раза, АЛТ – в 1,7 раза та їх співвідношення – у 2,4 раза). У щурів із гепатозом відмічено зниження синтезу факторів зсідання крові. Зокрема, вміст фібриногену в плазмі крові на тлі гепатозу знижувався на 21%, протромбіну – на 27,8%, фактора Xa – на 27,9%, протеїну С – на 40,6%. У цих тварин відзначали гіпохромемію як прояв анемії за розвитку гіперазотемії та гіпербілірубінемії. Виявлено порушення фосфорно-кальцієвого обміну та гіперкаліємію. Показано, що введення хворим тваринам фосфоліпідів молока у формі ліпосомальної біологічно активної добавки «FLP-MD» зменшувало прояв негативних ефектів тетрацикліну, про що свідчить відновлення рівня коагуляційних факторів та амінотрансферазної активності. Результати біохімічних досліджень дозволяють рекомендувати «FLP-MD» як засіб гепатопротекції
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10.

Заглавие журнала :The Ukrainian biochemical journal -2019г. т.91,N 1
Интересные статьи :
Varbanets L. D. Рantoea agglomerans lipopolysaccharides: structure, functional and biological activity/ L. D. Varbanets [та ін.] (стр.5-20)
Guzyk M. M. Altered sirtuins 1 and 2 expression in the brain of rats induced by experimental diabetes and the ways of its correction/ M. M. Guzyk [та ін.] (стр.21-29)
Galkin O.Yu. Different mice inbred strains humoral immune response against human prostate-specific antigen/ O. Yu. Galkin, A. G. Komar, O. B. Besarab (стр.30-37)
Minaev B. F. Spin-orbit coupling effects in O₂ activation by cofactor-independent 2,4-dioxygenase/ B. F. Minaev, R. R. Valiev (стр.38-46)
Onoiko O. B. Influence of sulfite on ATPase activity of coupling factor CF₁ isolated from spinach chloroplasts/ O. B. Onoiko, A. P. Khomochkin, O. K. Zolotareva (стр.47-52)
Sorochynska O. M. Intermittent fasting causes metabolic stress and leucopenia in young mice/ O. M. Sorochynska [та ін.] (стр.53-64)
Makarenko M. V. HBD-2 interactions with erythrocyte membranes in vitro/ M. V. Makarenko [та ін.] (стр.65-73)
Buznytska O. V. Diagnostic significance of biochemical indicators of liver fibrogenesis in adolescents with obesity/ O. V. Buznytska (стр.74-79)
Yelins’ka A. M. Role of AP-1 transcriptional factor in development of oxidative and nitrosative stress in periodontal tissues during systemic inflammatory response/ A. M. Yelins’ka, O. Ye. Akimov, V. O. Kostenko (стр.80-85)
Gudzenko T. V. Cellular fatty acid composition of Aeromonas genus – destructor of aromatic xenobiotics/ T. V. Gudzenko [та ін.] (стр.86-91)
Gryshchenko V. A. Evaluation of biochemical indicators in blood plasma of rats with tetracycline-induced hepatosis and their correction by milk phospholipids/ V. A. Gryshchenko [та ін.] (стр.92-99)
Petrenko O. M. Levels of angiogenic regulators and MMP-2, -9 activities in Martorell ulcer: a case report/ O. M. Petrenko, A. A. Tykhomyrov (стр.100-107)
Данилова В. М. Внесок лауреатів Нобелівської премії в розвиток динамічної біохімії та біоенергетики. Е. Бухнер, А. Коссель, Р. Вільштеттер, О. Мейєргоф, А. Хілл, О. Варбург, А. Сент-Дьєрді/ В. М. Данилова, Р. П. Виноградова, С. В. Комісаренко (стр.108-126)
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11.

Заглавие журнала :The Ukrainian biochemical journal -2018г. т.90,N 3
Интересные статьи :
Trakhtenberg I. M. Biochemical mechanisms of free-radical damage to the nuclear genome by cadmium/ I. M. Trakhtenberg [et al.] (стр.5-16)
Moshynets O. V. Examining c-di-GMP and possible quorum sensing regulation in Pseudomonas fluorescens SBW25: links between intra- and inter-cellular regulation benefits community cooperative activities such as biofilm formation/ O. V. Moshynets [et al.] (стр.17-31)
Babich L. G. The relation between the availability of neonatal intensive care and neonatal mortality./ L. G. Babich [et al.] (стр.32-40)
Franskevych D. V. Activation of store - operated Ca2+ entry in cisplatin resistant leukemic cells after treatment with photoexcited fullerene C60 and cisplatin/ D. V. Franskevych [et al.] (стр.41-48)
Minchenko O. H. ERN1 modifies the effect of glutamine deprivation on tumor growth related factors expression in U87 glioma cells/ O. H. Minchenko [et al.] (стр.49-61)
Gerush I. V. The effect of melationin on lipid peroxide oxidation, oxidative modification of proteins and mitochondria swelling in the skeletal muscle tissue of rats under alloxan diabetes/ I. V. Gerush, V. V. Bevzo, Ye. O. Ferenchuk (стр.62-69)
Galkin O. Yu. Development and characterization of highly informative elisa for the detection of IgG and IgA antibodies to Chlamydia trachomatis/ O. Yu. Galkin [et al.] (стр.70-83)
Ohiri R. C. Nutriceutical potential of Pleurotus tuber-regium sclerotium/ R. C. Ohiri (стр.84-93)
Horak I. R. Adaptor protein Ruk/CIN85 modulates resistance to doxorubicin of murine 4T1 breast cancer cells/ I. R. Horak, D. S. Geraschenko, L. B. Drobot (стр.94-100)
Modern aspects of biochemistry and biotechnology - 2018 (стр.101-147)
Horak I. R. Cellular plasticity as a driving force in cancer progression: the regulatory role of adaptor protein RuK/CIN85/ I. R. Horak [et al.] (стр.102-103)
Mazanova A. O. Modulating effect of cholecalciferol on vitamin D endo/para/autocrine system in type 1 diabetes/ A. O. Mazanova [et al.] (стр.104-105)
Uspenska K. R. Biological role of nicotinic acetylcholine receptors in mitochondria/ K. R. Uspenska (стр.106)
Chukova A. M. G-quadruplex DNA binding and telomerase inhibition by pheophorbides/ A. M. Chukova [et al.] (стр.107)
Demenko D. Computer modeling of direct factor Xa inhibitors/ D. Demenko, V. Hurmach, V. Chernyshenko (стр.108)
Dziuba O. S. The effect of N-stearoylethanolamine on age-related and diet-induced changes of fatty acid profile of rat adipocytes/ O. S. Dziuba [et al.] (стр.109)
Garmash I. A. Regulation of glycolysis-related genes expression in U87 ERN1 knockdown glioma cells/ I. A. Garmash (стр.110)
Gerashchenko D. S. Deficiency of adaptor protein RuK/CIN85 in lewis lung carcinoma cells inhibits malignancy hallmarks in vitro as well as tumor growth and pulmonary metastasis in vivo/ D. S. Gerashchenko [et al.] (стр.111)
Gorshkova O. G. Effective method for cleaning of ore of ore-ferrous factory from ions of heavy metals based on bacteria association of the genus Pseudomonas/ O. G. Gorshkova (стр.112)
Gritsenko M. Analyses of the expression and products accumulation of CD-marker genes in cultures of lung and skin rats fibroblasts in ontogenesis/ M. Gritsenko (стр.113)
Halkin O. V. Expression of protease genes in IRE1 knockdown U87 glioma cells upon glutamine deprivation/ O. V. Halkin, D. O. Minchenko, D. О. Tsymbal (стр.114)
Holiar V. V. P60-S6K1 mRNA transcript expression profile in a panel of cell lines and breast cancer tissue samples/ V. V. Holiar, A. S. Sivchenko, I. V. Zaiets (стр.115)
Kalmukova O. O. Central serotonin and tryptophan levels in rats with diet-induced obesity at the different time of melatonin administration/ O. O. Kalmukova, A. V. Yurchenko, V. M. Kyryk (стр.116)
Kharchenko T. The cytotoxicity of cadmium ions small doses in long-term culture of bone marrow cells/ T. Kharchenko [et al.] (стр.117)
Kharchuk M. S. The composition of isolated volutin granules of Saccharomyces cerevisiae UCM Y-517/ M. S. Kharchuk (стр.118)
Klymets H. V. The effect of vanadium citrate on the biochemical parameters of the blood plasma of pregnant female rats/ H. V. Klymets (стр.119)
Kolesnikov Ya. S. The role of brassinosteroids in regulation of phospholipid signaling in plant cells/ Ya. S. Kolesnikov, S. V. Kretynin (стр.120)
Korchevska V. V. Expression profile of fatty acids in the liver and guerin’s carcinoma of rats under conditions of ?-3 polyunsaturated fatty acids administration/ V. V. Korchevska, O. V. Ketsa, V. M. Klimashevskyi (стр.121)
Kovalchuk Y. P. The myelin basic protein and S100b level in the different brain areas of rats affected by pituitrin and izadrin/ Y. P. Kovalchuk (стр.122)
Labudzynskyi D. O. Effects of cerium (IV) oxide nanoparticles on RAW 264.7 cells activity and rankl-stimulated osteoclastogenesis/ D. O. Labudzynskyi (стр.123)
Luzina O. Y. Effect of glutamine deprivation on the expression of DEK, TPD52, BRCA1, ADGRE5, LIF, GNPDA1, and COL6A1 genes in IRE1 knockdown U87 glioma cells/ O. Y. Luzina (стр.124)
Marenkov O. N. The effect of manganese, nickel and lead ions on LDH activity of the Procambarus virginalis (LYKO, 2017)/ O. N. Marenkov, Y. P. Kovalchuk (стр.125)
Melnyk T. Hepatoprotective effects of ?-3 polyunsaturated fatty acids on rats with transplanted guerin’s carcinoma/ T. Melnyk, O. Ketsa (стр.126)
Muraviova D. V. The neural cell adhesion molecules in the rat brain after water-immobilization stress and after the recovery period/ D. V. Muraviova, O. O. Dovban (стр.127)
Paliienko K. O. Dose-dependent action of polyhexamethylene guanidine hydrochloride on release of L-[14C]glutamate from rat brain nerve terminals/ K. O. Paliienko, A. O. Pastukhov (стр.128)
Pankiv T. M. Effects of intermittent fasting on respiratory capacity of mitochondria and activity of aconitase in cerebral cortex of C57BL/6 mice/ T. M. Pankiv (стр.129)
Revka O. V. Biochemical mechanisms of fibrin clot formation and subsequent lysis regulation by platelets/ O. V. Revka (стр.130)
Riabovol O. O. The expression of cox and NDUF genes in U87 glioma cells with IRE1 knockdown/ O. O. Riabovol (стр.131)
Rutska A. V. The influence of monosodium glutamate on reactive oxygen species production in rats/ A. V. Rutska (стр.132)
Rybak M. Yu. The role of 2’- and 3’-hydroxyl groups of A76 tRNATyr at the first steps of translation quality control/ M. Yu. Rybak (стр.133)
Gorshkova O. G. Features of fatty strength profile of strain Brevibacillus centrosporus F14 - destructor of phenolic compounds/ O. G. Gorshkova [et al.] (стр.134)
Si W. The hemolytic effect of oxidative stress caused by chronic exposure to low concentrations of cadmium on the body/ W. Si (стр.135)
Sishchuk L. O. The activity of glycolytic and antiglycative enzymes in mouse brain under intermittent fasting/ L. O. Sishchuk, A. O. Semchuk (стр.136)
Spirina V. A. Biochemical parameters of blood with increasing concentration of nitric oxide/ V. A. Spirina, O. O. Galinskij (стр.137)
Stohniy Y. M. The development of new method of the determination of bacterial transglutaminase activity using fibrinogen as a substrate/ Y. M. Stohniy [et al.] (стр.138)
Stravska M. Redox balance in white rats’ spleen in the dynamics of experimentally developed carcinogenesis/ M. Stravska [et al.] (стр.139)
Suleimanova R. R. Fatty acids composition in blood serum lipids of sterlets of different age/ R. R. Suleimanova (стр.140)
Sushko O. Glutathione status in rat’s liver experimentally induced under influence chromium citrate/ O. Sushko, L. Ponkalo (стр.141)
Tsap P. Y. Mapping of residues of fibribogen ?C-region cleaved by protease from the venom of Agkistrodon halys halys/ P. Y. Tsap [et al.] (стр.142)
Umanska A. O. Changes of the performance parameters of the heart of rats for artificial hypobyosis/ A. O. Umanska (стр.143)
Vasheniuk O. S. Adaptor protein Ruk/CIN85 induces epithelial-to-mesenchymal transition in human A549 lung adenocarcinoma cells/ O. S. Vasheniuk [et al.] (стр.144)
Zhuk V. V. Signal function of endogenous hydrogen peroxide in response of plants to ultraviolet radiation/ V. V. Zhuk (стр.145)
Zhuk I. V. The combination of no donor and ferulic acid effect on the elicitation of wheat tolerance against biotic stress/ I. V. Zhuk, L. O. Kucherova (стр.146)
Zhyvolozhnyi A. Y. Adaptor protein Ruk/CIN85 is a novel molecular component of extracellular vesicles produced by tumor cells/ A. Y. Zhyvolozhnyi [et al.] (стр.147)
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dziuba O. S., Chernyshenko V. O., Hudz Ie. A., Kasatkina L. O., Chernyshenko T. M., Klymenko P. P., Kosiakova H. V., Platonova T. M., Hula N. M., Lugovskoy E. V.
Заглавие : Blood coagulation and aortic wall integrity in rats with obesity-induced insulin resistance
Место публикации : The Ukrainian Biochemical Journal. - Київ, 2018. - Том 90, N 2. - С. 14-24 (Шифр УУ60/2018/90/2)
MeSH-главная: ОЖИРЕНИЕ -- OBESITY
АТЕРОСКЛЕРОЗ -- ATHEROSCLEROSIS
ИНСУЛИНОРЕЗИСТЕНТНОСТЬ -- INSULIN RESISTANCE
ЭУКАРИОТЫ -- EUKARYOTA
Аннотация: Obesity is an important factor in pathogenesis of disorders caused by chronic inflammation. Diet-induced obesity leads to dyslipidemia and insulin resistance (IR) that in turn provoke the development of type 2 diabetes and cardiovascular diseases. Thus, the aim of this work was to investigate the possible pro-atherogenic effects in the blood coagulation system and aortic wall of rats with obesity-induced IR. The experimental model was induced by a 6-month high-fat diet (HFD) in white rats. Blood samples were collected from 7 control and 14 obese IR rats. Prothrombin time (PT) and partial activated thromboplastin time (APTT) were performed by standard methods using Coagulometer Solar СТ 2410. Fibrinogen concentration in the blood plasma was determined by the modified spectrophotometric method. Levels of protein C (PC), prothrombin and factor X were measured using specific chromogenic substrates and activating enzymes from snake venoms. Platelet aggregation was measured and their count determined using Aggregometer Solar AP2110. The aorta samples were stained by hematoxylin and eosin according to Ehrlich. Aortic wall thickness was measured using morphometric program Image J. Statistical analysis was performed using Mann-Whitney U Test. The haemostasis system was characterized by estimation of the levels of individual coagulation factors, anticoagulant system involvement and platelet reactivity. PT and APTT demonstrated that blood coagulation time strongly tended to decrease in obese IR rats in comparison to the control group. It was also detected that 30% of studied obese IR rats had decreased factor X level, 40% had decreased level of prothrombin whereas fibrinogen concentration was slightly increased up to 3 mg/ml in 37% of obese IR rats. A prominent decrease of anticoagulant PC in blood plasma of obese rats was detected. Obese IR rats also had increased platelet count and higher rate of platelet aggregation in comparison to control animals. Histological analysis identified the disruption of aorta endothelium and tendency for the thickening of the aorta wall in the group with obesity-induced IR compared to the group of control rats. Changes of individual coagulation factors were assumed as the evidence of imbalance in the blood coagulation system. Increase of fibrinogen level, drop in PC concentration and pathological platelet reactivity were taken to corroborate the development of low-grade inflammation in obese IR rats. Instant generation of small amounts of thrombin in their blood plasma is expected. Since the aorta morphology assay detected the trend of its wall to thicken and the emergence of disruptions, we assumed there were initial stages of atherosclerosis and the danger of developing atherothrombosis. We detected an increase of blood coagulability and changes in aorta morphology in rats with obesity-induced IR which we assume indicate early development of atherosclerosis
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13.

Заглавие журнала :The Ukrainian biochemical journal -2018г. т.90,N 2
Интересные статьи :
Sandor G. Vari. Knowledge sharing is the key for the progress of science/ G. Vari Sandor (стр.5-7)
Paryzhak S. Ya. Simple two-step covalent protein conjugation to PEG-coated nanocrystals/ S. Ya. Paryzhak [et al.] (стр.8-13)
Dziuba O. S. Blood coagulation and aortic wall integrity in rats with obesity-induced insulin resistance/ O. S. Dziuba [et al.] (стр.14-24)
Finiuk N. S. Evaluation of antiproliferative activity of pyrazolothiazolopyrimidine derivatives/ N. S. Finiuk [et al.] (стр.25-33)
Hudz N. Biological and analytical studies of peritoneal dialysis solutions/ N. Hudz [et al.] (стр.34-45)
Kozak Yu. S. Impact of N-acetylcysteine on antitumor activity of doxorubicin and landomycin A in NK/Ly lymphoma-bearing mice/ Yu. S. Kozak [et al.] (стр.46-55)
Labudzynskyi D. O. Osteoprotective effects of vitamin D(3) in diabetic mice is VDR-mediated and regulated via RANKL/RANK/OPG axis/ D. O. Labudzynskyi [et al.] (стр.56-66)
Patalakh I. I. Clot formation and lysis in platelet rich plasma of healthy donors and patients with resistant hypertension/ I. I. Patalakh [et al.] (стр.67-77)
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chernyshenko V. O., Savchuk O. V., Cherenok S. O., Silenko O. M., Negelia A. O., Kasatkina L. O., Pirogova L. V., Didkivskyi V. A., Yusova O. I., Kalchenko V. I., Garmanchuk L. V., Grinenko T. V., Lugovskoy E. V., Komisarenko S. V.
Заглавие : Haemostasis modulation by calix[4]arene methylenebisphosphonic acid C-145 and its sulfur-containing analogue
Место публикации : The Ukrainian Biochemical Journal. - Київ, 2018. - Том 90, N 6. - С. 21-30 (Шифр УУ60/2018/90/6)
MeSH-главная: ГЕМОСТАЗ -- HEMOSTASIS
ФИБРИНОЛИЗ -- FIBRINOLYSIS
СВЕРТЫВАНИЕ КРОВИ -- BLOOD COAGULATION
ФИБРИНОЛИТИЧЕСКИЕ СРЕДСТВА -- FIBRINOLYTIC AGENTS
Аннотация: C-145 (octasodium salt of calix[4]arene-tetra-methylenebisphosphonic acid) was previously considered as specific anti-сoagulant agent that affects fibrin polymerization and does not notably influence other parameters of coagulation system. C-145S (octasodium salt of thiacalix[4]arene-tetra-methylenebisphosphonic acid) possessing wider hydrophobic hole was expected to be more effective antithrombotic agent than C-145. The aim of present work was to compare the action of both organic compounds on fibrin polymerization, fibrinolysis, platelets and endothelial cells. The change of turbidity during fibrin clot formation induced by APTT-reagent and digestion induced by tPA was estimated. Turbidity study was used for the estimation of polymeric fibrin hydrolysis by plasmin in the presence of thiacalix[4]arene C-145S and calix[4]arene C-145. Effects of thiacalix[4]arene C-145S and calix[4]arene C-145 on the activation of Glu-plasminogen by streptokinase were studied using chromogenic substrate S2251. Platelet aggregation study was performed using aggregometry. Stimulated Ca2+ efflux from endoplasmic reticulum and cytoplasm were determined using specific Ca2+-sensitive probes targeted to endoplasmic reticulum (Mag-Fluo-4) and cytoplasm (FURA-2) by spectrofluorimetry. Both C-145 and C-145S decreased the final turbidity of clot and prolonged clot lysis time in blood plasma in comparison to control value. C-145 was shown to be the more effective fibrinolysis inhibitor when studied in model system of polymerized fibrin desAB. C-145S but not C-145 induced concentration changes of Ca2+ in cytoplasm of resting platelets and significantly inhibited (up to 30%) Ca2+ efflux from endoplasmic reticulum of platelets activated by ADP. Both C-145 and C-145S stimulated the proliferation of endothelial cells of PAE cell line. The effect of C-145S was more prominent. In conclusion, calix[4]arene C 145S proved to be the more potent inhibitor of fibrin polymerization in comparison to C-145, which suggested earlier as anticoagulant agent. C-145S proved to have much more outlined inhibitory action on Ca2+-signaling in platelets and stimulatory effect on endothelial cells proliferation. Thus C-145 remained the most prospective molecular platform for the development of antithrombotic agent
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15.

Заглавие журнала :The Ukrainian biochemical journal -2018г. т.90,N 6
Интересные статьи :
Asmaa Missoum. The role of gene GJB2 and connexin 26 in hearing impairment/ Missoum Asmaa (стр.5-11)
Kolupaev Yu. E. Phenylalanine ammonia-lyase activity and content of flavonoid compounds in wheat seedlings at the action of hypothermia and hydrogen sulfide donor/ Yu. E. Kolupaev [et al.] (стр.12-20)
Chernyshenko V. O. Haemostasis modulation by calix[4]arene methylenebisphosphonic acid C-145 and its sulfur-containing analogue/ V. O. Chernyshenko [et al.] (стр.21-30)
Tykhomyrov A. A. Plasminogen modulates formation of reactive oxygen species in human platelets/ A. A. Tykhomyrov [et al.] (стр.31-40)
Siromolot A. A. Specificity and sensitivity of the new test for serological evaluation of tuberculosis using MPT83-MPT63 fusion antigen/ A. A. Siromolot [et al.] (стр.41-48)
Lylyk M. P. Effects of alpha-ketoglutarate on lifespan and functional aging. Of Drosophila melanogaster flies/ M. P. Lylyk [et al.] (стр.49-61)
Purkan P. Immunogenicity assay of KatG protein from Mycobacterium tuberculosis in mice: preliminary screening of TB vaccine/ P. Purkan [et al.] (стр.62-69)
Zavodovskyi D. O. Influence of C60 fullerene on the ischemia-reperfusion injury in the skeletal muscle of rat limb: mechanokinetic and biochemical analysis/ D. O. Zavodovskyi [et al.] (стр.70-81)
Nakonechna O. A. Disturbance of the transmembrane phosphatidylserine asymmetry in hepatocytes as an apoptosis marker under the action of xenobiotics on rats/ O. A. Nakonechna, L. A. Babijchuk, A. I. Bezrodna (стр.82-88)
Aminizadeh S. The effects of PDK4 inhibition on AMPK protein levels and PGC-1α gene expression following endurance training in skeletal muscle of Wistar rats/ S. Aminizadeh, Y. Masoumi-Ardakani, B. Shahouzehi (стр.89-96)
Horid’ko T. M. Preventive effect of N-stearoylethanolamine on memory disorders, blood and brain biochemical parameters in rats with experimental scopolamine-induced cognitive impairment/ T. M. Horid’ko [et al.] (стр.97-109)
Hurmach Y. The effect of intranasally administered TLR3 agonist larifan on metabolic profile of microglial cells in rat with C6 glioma/ Y. Hurmach [et al.] (стр.110-119)
Marounek M. Long-term hypocholesterolemic effect of amidated alginate in rats/ M. Marounek [et al.] (стр.120-125)
Данилова В. М. Внесок Нобелівських лауреатів початку ХХ ст. в розвиток молекулярної імунології: Е. Берінг, І. І. Мечников, П. Ерліх, Ш. Ріше, Ж. Борде, К. Ландштейнер/ В. М. Данилова, Р. П. Виноградова, С. В. Комісаренко (стр.126-142)
Дмитро Олексійович Мельничук. До 75-річчя з дня народження (стр.143-144)
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Stohniy Y. M., Gryshchuk V. I., Chernyshenko V. O., Chernyshenko T. M., Hrushetska I. V.
Заглавие : The development of new method of the determination of bacterial transglutaminase activity using fibrinogen as a substrate
Место публикации : The Ukrainian Biochemical Journal. - Київ, 2018. - Том 90, N 3. - С. 138 (Шифр УУ60/2018/90/3)
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17.

Заглавие журнала :Український біохімічний журнал -2016г. т.88,N 3
Интересные статьи :
Toptikov V. A. Hydrolytic enzymes expressivity in different parts of the Rapana digestive system/ V. A. Toptikov [и др.] (стр.5-17)
Nidialkova N. А. Isolation and purification of Bacillus thuringiensis var. israelensis IМV В-7465 peptidase with specificity toward elastin and collagen/ N. А. Nidialkova, L. D. Varbanets, V. O. Chernyshenko (стр.18-28)
Bobrovnik S. A. Kinetic parameters of polyreactive immunoglobulins interaction with antigens in the presence of protamine/ S. A. Bobrovnik, M. O. Demchenko, S. V. Komisarenko (стр.29-35)
Grinenko T. V. Plasminogen fragments K 1-3 and K 5 bind to different sites in fibrin fragment DD/ T. V. Grinenko [и др.] (стр.36-45)
Bezkorovaynyj A. O. Loach embryos prooxidant-antioxidant status under the influence of amide derivatives of 1,4-naphthoquinone/ A. O. Bezkorovaynyj [и др.] (стр.46-53)
Minchenko O. H. Effect of hypoxia on the expression of nuclear genes encoding mitochondrial proteins in U87 glioma cells/ O. H. Minchenko [и др.] (стр.54-65)
Minchenko O. H. Expression of IGFBP6, IGFBP7, NOV, CYR61, WISP1 and WISP2 genes in U87 glioma cells in glutamine deprivation condition/ O. H. Minchenko [и др.] (стр.66-77)
Zagayko A. L. The impact of hydroxycitric acid on the lipid metabolism profile under experimental insulin resistance syndrome of Syrian hamsters/ A. L. Zagayko [и др.] (стр.78-82)
Sokolik V. V. Effect of curcumin on accumulation in mononuclear cells and secretion in incubation medium of Аβ(40) and cytokines under local excess of Аβ(42)-homoaggregates/ V. V. Sokolik, S. M. Shulga (стр.83-91)
Khyzhnyak S. V. Fatty acids composition of inner mitochondrial membrane of rat cardiomyocytes and hepatocytes during hypoxia-hypercapnia/ S. V. Khyzhnyak [и др.] (стр.92-98)
Shmarakov I. O. Hepatotoxicity of bisphenol A under conditions of differential supplementation with retinoids/ I. O. Shmarakov [и др.] (стр.99-105)
Луговська Н. Е. Винахідницька діяльність відділів структури і функції білка та молекулярної імунології Інституту біохімії ім. О. В. Палладіна НАН України. Частина ІІ. Вітчизняний прорив у дослідженні та діагностиці системи гемостазу людини/ Н. Е. Луговська (стр.106-118)
Надія Максимівна ГУЛА. До 80-річчя з дня народження (стр.119-120)
Олександр Григорович МІНЧЕНКО. До 70-річчя з дня народження (стр.121-122)
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nidialkova N. А., Varbanets L. D., Chernyshenko V. O.
Заглавие : Isolation and purification of Bacillus thuringiensis var. israelensis IМV В-7465 peptidase with specificity toward elastin and collagen
Параллельн. заглавия :Виділення та очистка пептидази Bacillus thuringiensis var. israelensis ІМВ В-7465 зі специфічністю до еластину і колагену
Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 3. - С. 18-28 (Шифр УУ1/2016/88/3)
Примечания : Bibliogr. at the end of the art.
MeSH-главная: КОЛЛАГЕН -- COLLAGEN
BACILLUS THURINGIENSIS -- BACILLUS THURINGIENSIS
ФИЗИКО-ХИМИЧЕСКИЕ ПРОЦЕССЫ -- PHYSICOCHEMICAL PROCESSES
ЭЛАСТИН -- ELASTIN
Аннотация: Peptidase of Bacillus thuringiensis var. israelensis IМV В-7465 was isolated from culture supernatant using consecutive fractionations by an ammonium sulphate (60% saturation), ion-exchange chromatography and gel-filtration on the TSK-gels Toyoperl HW-55 and DEAE 650(M). Specific elastase (442 U∙mg of protein-1) and collagenase (212.7 U∙mg of protein-1) activities of the purified enzyme preparation were 8.0- and 6.1-fold, respectively higher than ones of the culture supernatant. Peptidase yields were 33.5% for elastase activity and 30.1% for collagenase activity. It was established that the enzyme is serine metal-dependent alkaline peptidase with Mr about 37 kDa. Maximal hydrolysis of elastin and collagen occurs at the optimum pH 8.0 and t° – 40 and 50 °С, respectively. The purified preparation has high stability at pH in the range of 7.0 to 10.0 and 40-50 °С. Пептидаза Bacillus thuringiensis var. israelensis ІМВ В-7465 виділена із супернатанта культуральної рідини методами послідовного фракціонування сульфатом амонію (60% насичення), іонообмінної хроматографії та гель-фільтрації на TSK-гелях – Toyoperl HW-55 і DEAE 650(M). Питома еластазна (442 од∙мг протеїну-1) і колагеназна (212,7 од∙мг протеїну-1) активність очищеного ензимного препарату була відповідно в 8,0 і 6,1 раза вищою порівняно із супернатантом культуральної рідини. При цьому вихід пептидази за еластазною активністю сягав 33,5%, за колагеназною – 30,1%. Показано, що ензим є сериновою металозалежною лужною пептидазою з Мм ~ 37 кДа. Максимальний гідроліз еластину і колагену відбувається за оптимальних значень рН – 8,0 і температури – 40 і 50 °С відповідно. Показано високу стабільність очищеного препарату у разі рН від 7,0 до 10,0 і 40–50 °С протягом 2 годин
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