Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Komisarenko, S. V.
Prospects of genome editing using CRISPR/CAS or how to master genetic scissors. Nobel Prize in chemistry 2020 / S. V. Komisarenko, S. I. Romaniuk // The Ukrainian biochemical journal. - 2021. - Vol. 93, № 1. - P113-128

MeSH-главная:
ХИМИЯ -- CHEMISTRY (тенденции)
МОЛЕКУЛЯРНАЯ БИОЛОГИЯ -- MOLECULAR BIOLOGY (тенденции)
ГЕННАЯ ИНЖЕНЕРИЯ -- GENETIC ENGINEERING (тенденции)
НОБЕЛЕВСКАЯ ПРЕМИЯ -- NOBEL PRIZE
Аннотация: The Nobel Prize in Chemistry 2020 was awarded to two researchers in the field of molecular biology – the French Emmanuelle Charpentier, who now heads the Max Planck Unit for the Science of Pathogens in Berlin, and the American Jennifer Doudna from the University of California, Berkeley – for the ‘development of genome editing method’. A press release of the Nobel Committee states that the winners discovered one of the most powerful genetic technology tools – CRISPR/Cas9, or so-called ‘genetic scissors’. This method has contributed to many important results of basic research. In particular, plant researchers have managed to create crops resistant to mold, pests and drought. As for medicine, clinical trials of new cancer treatment techniques are underway, and a dream of curing hereditary diseases is about to become a reality. Genetic scissors have brought the life sciences to a new stage of development and greatly contributed to the benefit of mankind
Нобелівську премію з хімії у 2020 р. присуджено двом дослідницям у галузі молекулярної біології – француженці Еммануель Шарпантьє (Emmanuelle Charpentier), яка нині очолює Відділення наук про патогени при Товаристві Макса Планка в Берліні, та американці Дженніфер Дудні (Jennifer Doudna) з Каліфорнійського університету в Берклі – за «розвиток методу редагування геному». У пресрелізі Нобелівського комітету зазначено, що лауреатки відкрили один з найпотужніших інструментів генної технології – CRISPR/Cas9 – або так звані «генетичні ножиці». Цей метод сприяв отриманню у фундаментальних дослідженнях багатьох важливих результатів. Зокрема, дослідники рослин змогли створити культури, стійкі до цвілі, шкідників та посухи. У медицині тривають клінічні випробування нових методів лікування раку, а мрія про те, щоб вилікувати спадкові захворювання, ось-ось стане реальністю. «Генетичні ножиці» вивели науки про життя на новий етап розвитку і дають людству величезну користь
Доп.точки доступа:
Romaniuk, S. I.

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Шифр: УУ60/2021/93/1
Журнал

The Ukrainian biochemical journal [Текст]. - Выходит раз в два месяца
2021г. Т. 93 № 1

Содержание:
Zaloilo, I. Coronavirus viroporins: structure and function / I. Zaloilo [et al.]. - P.5-17
Другие авторы: Rud Y., Zaloilo O., Buchatskyi L.
Meftahi, G. H. A vicious circle between oxidative stress and cytokine storm in acute respiratory distress syndrome pathogenesis at Covid-19 infection / G. H. Meftahi [et al.]. - P.18-29
Другие авторы: Bahari Z., Jangravi Z., Iman M.
Kirana, A. N. Protein intake and loss of proteostasis in the eldery / A. N. Kirana, E. Prafiantini, N. S. Hardiany. - P.30-39
Sultanova, A. S. Stromal-vascular fraction of adipose tissue as an alternative source of cellular material for regenerative medicine / A. S. Sultanova, O. Ya. Bespalova, O. Yu. Galkin. - P.40-50
Ragy, M. M. Leptin and curcumin affect renal ischemia-reperfusion injury via modulation of P65 and bax genes expression / M. M. Ragy, M. M. Ramzy. - P.51-58
Gholami, M. Effect of N-acetyl cysteine on oxidative stress and Bax and Bcl2 expression in the kidney tissue of rats exposed to lead / M. Gholami [et al.]. - P.59-67
Другие авторы: Harchegani A. B., Saeedian S., Owrang M., Parvizi M. R.
Grabovsky, S. S. Male and female rats differ in homeostatic shifts during pre-slaughter fear stress / S. S. Grabovsky [et al.]. - P.68-74
Другие авторы: Panchuk R. R., Skorokhyd N. R., Stoika R. S.
Smirnov, O. E. Organo-specific accumulation of phenolic compounds in a buckwheat seedlings under alum-acid stressinium / O. E. Smirnov [et al.]. - P.75-81
Другие авторы: Kosyan A. M., Pryimak Yu. V., Kosyk O. I., Taran N. Yu.
Orcun, Avsar. MicroRNAs hsa-mir-34a and hsa-mir-124 as biomarkers for predicting and monitoring the lithium treatment in bipolar disorder: in silico analysis / Avsar Orcun. - P.82-87
Skachkova, O. V. Comparative characteristic of lung cancer stem-like cells generated in vitro under different culture conditions / O. V. Skachkova [et al.]. - P.88-95
Другие авторы: Gorbach O. I., Inomistova M. V., Garmanchuk L. V., Khranovska N. M.
Harasym, N. P. Quercetin and histamine effects on free radical reactions in rat erythrocytes / N. P. Harasym [et al.]. - P.96-103
Другие авторы: Booklyv M. Y., Zyn A. R., Mandzynets S. M., Bezkorovainy A. O., Sanahursky D. I.
Ostapiv, D. D. Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases / D. D. Ostapiv [et al.]. - P.104-112
Другие авторы: Kuz’mina N. V., Kozak M. R., Hu Shan, Vlizlo V. V., Kotsiumbas I. Ya., Varvarenko S. M., Samaryk V. Ya., Nosova N. G., Yakoviv M. V.
Komisarenko, S. V. Prospects of genome editing using CRISPR/CAS or how to master genetic scissors. Nobel Prize in Chemistry 2020 / S. V. Komisarenko, S. I. Romaniuk. - P.113-128
Матишевська, О. П. Відкриття механізмів біологічного синтезу нуклеїнових кислот: нобелівські лауреати 1959 р. С. Очоа і А. Корнберг / О. П. Матишевська, В. М. Данилова, С. В. Комісаренко. - P.129-138
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Danylova, T. V.
Double Nobel prize winner: Frederick Sanger - the father of genomics / T. V. Danylova, S. V. Komisarenko // The Ukrainian biochemical journal. - 2021. - Vol. 93, № 2. - P116-122

MeSH-главная:
НОБЕЛЕВСКАЯ ПРЕМИЯ -- NOBEL PRIZE
Аннотация: This paper aims to outline briefly the main stages of Frederick Sanger’s scientific activity – the only person to have won two Nobel Prizes in Chemistry (1958, 1980). His work on the structure of proteins, especially that of insulin, and the determination of base sequences in nucleic acids made an immense impact on the development of biochemistry and especially on the development of a new scientific field – molecular biology. His methods for determining the primary structure of proteins and nucleic acids helped biochemists and molecular biologists to determine the structure of many proteins and nucleic acids and laid the basis for genetic engineering
Доп.точки доступа:
Komisarenko, S. V.
Sanger, Frederick (13.08.1918-19.11.2013) \о нем\

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Шифр: УУ60/2021/93/2
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The Ukrainian biochemical journal [Текст]. - Выходит раз в два месяца
2021г. Т. 93 № 2

Содержание:
Vari, S. G. Inflammation is the common mechanism of diseases (CMD) in COVID-19 disease during pregnancy and in gestational diabetes mellitus / S. G. Vari. - P.5
Kobylinska, L. In silico identification and biochemical validation of plausible molecular targets of 4-thiazolidinone derivative les-3833 as a potential anticancer agent / L. Kobylinska [et al.]. - P.7-23
Другие авторы: Khylyuk D., Subtelna I., Kitsera M., Lesyk R.
Shevchuk, O. O. Locally delivered lovastatin-containing chitosan nanoparticles promote bone regeneration in rats / O. O. Shevchuk, Ya. V. Panasiuk, M. M. Korda. - P.24-38
Finiuk, N. S. Effect of a novel thiazole derivative and its complex with a polymeric carrier on stability of DNA in human breast cancer cells / N. S. Finiuk [et al.]. - P.39-52
Другие авторы: Klyuchivska O. Yu., Ivasechko I. I., Mitina N. E., Ostapiuk Yu. V., Obushak M. D., Zaichenko O. S., Babsky A. M., Stoika R. S.
Poshyvak, O. B. Oxidative stress suppression contributes to antiseizure action of axitinib and rapamycin in pentylenetetrazol-induced kindling / O. B. Poshyvak, O. R. Pinyazhko, L. S. Godlevsky. - P.53-60
Korolova, D. S. Thromboelastographic study of fibrin clot and molecular basis of maximum clot firmness / D. S. Korolova [et al.]. - P.62-70
Другие авторы: Stohnii Y. M., Gryshchuk V. I., Zhuk S. I., Us I. V., Chernyshenko T. M., Kostiuchenko O. P., Klymenko K. P., Platonov O. M., Ivashchenko O. I., Chernyshenko V. O.
Pavlyshyn, H. A. The association between vitamin D status and cardiometabolic disorders in overweight and obese adolescents / H. A. Pavlyshyn, A.-M. A. Shulha. - P.72-81
Pavlyshyn, H. A. Investigating the connections between night eating syndrome and metabolic syndrome in children / H. A. Pavlyshyn, K. V. Kozak, K. T. Hlushko. - P.83-94
Hlushko, K. T. Clinical and immunological peculiarities of parasitic infections in children with digestive system disorders in Western Ukraine / K. T. Hlushko, H. A. Pavlyshyn, K. V. Kozak. - P.96-105
Kucher, S. V. Inclusion of enterosorbents in anti-inflammatory therapy improve treatment effectiveness in COPD patients during exacerbations / S. V. Kucher, O. V. Lototska. - P.107-114
Danylova, T. V. Double Nobel prize winner: Frederick Sanger - the father of genomics / T. V. Danylova, S. V. Komisarenko. - P.116-122
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Aggregation of platelets, proliferation of endothelial cells and motility of cancer cells are mediated by the Bβ1(15)-42 residue of fibrin(ogen) / Y. M. Stohnii [et. al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 2. - P72-85

MeSH-главная:
ФИБРИНОГЕН -- FIBRINOGEN (метаболизм)
НОВООБРАЗОВАНИЙ ИНВАЗИВНОСТЬ -- NEOPLASM INVASIVENESS (патофизиология)
Аннотация: The fibrinogen molecule contains multiple binding motifs for different types of cellular receptors, acting as a molecular link between coagulation and cell adhesion. In this study we generated a truncated form of the fibrinogen molecule lacking the Bβ1-42 sequence by site-specific proteolysis and evaluated the role of the fragment in adhesive capabilities of platelets, endothelial and cancer cells. Fibrinogen with the removed Bβ1-42 sequence and fibrin without the Bβ15-42 fragment (desβ1-42 fibrinogen and desABβ15-42 fibrin) were obtained by proteolysis using the specific protease from the venom of Echis multisquamatis. The cleaved fragment was purified by HPLC and was identified using MALDI-TOF. ADP- and collagen-induced aggregation of washed platelets in the presence of fibrinogen desBβ1-42 was studied using an aggregometer. Proliferation of mice aortic endothelial cells (MAEC) and human umbilical vein endothelial cells (HUVEC) was studied using the fibrin desABβ15-42 as the scaffold. Cell viability was quantified by the MTT test (MAEC). Generation time was calculated for the estimation of proliferative activity of HUVEC. Lung cancer cell line Н1299 was used to evaluate cancer cell motility in vitro using the scratch assay. Direct comparison of cellular behavior in the presence of truncated vs native forms demonstrated attenuated cell adhesion in the presence of fibrinogen desBβ1-42 and fibrin desBβ15-42. The platelet aggregation rate was only slightly decreased in the presence of fibrinogen desBβ1-42 but resulted in 15-20% disaggregation of adhered platelets. We also observed the substantial decrease of generation time of HUVEC and inhibition of viability of MAEC cells grown on scaffolds of a desABβ15-42 matrix. Finally, desBβ1-42 modulated the motility of H1299 cells in vitro and suppressed the wound healing by 20% compared to the full-length fibrinogen. We postulate that fragment 1-42 of the BβN-domain of fibrinogen is not sufficient for platelet aggregation, however it may contribute to platelet clot formation in later stages. At the same time, this fragment may be important for establishing proper cell-to-cell contacts and cell viability of endothelial cells. Also, 1-42 amino acid fragment of the BβN-domain supported the migration of cancer cells suggesting that interactions of fibrinogen with cancer cells could be a target for anticancer therapy. The Bβ1-42 fragment of fibrinogen contributes to efficient intracellular interactions of different types of cells, including platelets, endothelial cells and cancer cells
Доп.точки доступа:
Stohnii, Y. M.
Ryzhykova, M. V.
Rebriev, A. V.
Kuchma, M. D.
Marunych, R. Y.
Chernyshenko, V. O.
Shablii, V. A.
Lypova, N. M.
Slominskyi, O. Yu.
Garmanchuk, L. V.
Platonova, T. M.
Komisarenko, S. V.

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Шифр: УУ60/2020/92/3
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The Ukrainian biochemical journal [Текст]. - Выходит раз в два месяца
2020г. Т. 92 № 3

Содержание:
Chernyshenko, V. Presenting thematic ’Molecular and clinical studies of hemostasis’ issue / V. Chernyshenko, S. Komisarenko. - P.5
Vari, S. G. COVID-19 infection: disease mechanism, vascular dysfunction, immune responses, markers, multiorgan failure, treatments, and vaccination / S. G. Vari : 21
Medved, L. Structure and function of fibrinogen BβN-Domains / L. Medved, S. Yakovlev. - P.22-32
Lugovskoi, E. The fibrin Bβ125-135 site is involved in the lateral association of protofibrils / E. Lugovskoi [et al.]. - P.33-45
Другие авторы: Pydiura N., Makogonenko Y., Urvant L., Gritsenko P., Kolesnicova I., Lugovska N., Komisarenko S.
Kapustianenko, L. G. Identification of the binding site for plasminogen kringle 5 in the α-chain of fibrin(ogen) D-fragment / L. G. Kapustianenko [et al.]. - P.46-57
Другие авторы: Grinenko T. V., Rebriev A. V., Yusova O. I., Tykhomyrov A. A.
Lugovska, N. E. Novel monoclonal antibody to fibrin(ogen) αC-region for detection of the earliest forms of soluble fibrin / N. E. Lugovska [et al.]. - P.58-70
Другие авторы: Kolesnikova I. M., Stohnii Ye. M., Chernyshenko V. O., Rebriev A. V., Kostiuchenko O. P., Gogolinska G. K., Dziubliuk N. A., Varbanets L. D., Platonova T. M., Komisarenko D. V.
Mykhaloiko, I. S. D-dimer as a potential predictor of thromboembolic and cardiovascular complications in patients with chronic kidney disease / I. S. Mykhaloiko [et al.]. - P.71-76
Другие авторы: Dudar I. O., Mykhaloiko I. Ja., Mykhaloiko O. Ja.
Panibratiuk, O. A. Warfarin therapy in patients with coronary heart disease and atrial fibrillation: drug interactions and genetic sensitivity to warfarin / O. A. Panibratiuk, O. A. Yakovleva. - P.77-85
Chernyshenko, V. O. Men of the molecules / V. O. Chernyshenko. - P.86-90
Григор’єва, М. В. 20 років тому завершилися "перегони" секвенування геному людини / М. В. Григор’єва ; М. В. Григор’єва, С. В. Комісаренко. - P.91-92
Danylova, T. V. Nobel prize winner erwin schrodinger: the physicist, philosopher, and godfather of molecular biology and genetics / T. V. Danylova, S. V. Komisarenko. - P.93-100
До 70-річчя від дня народження члена-кореспондента НАН України, професора Ростислава Стефановича Стойки. - P.101-102
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Шифр: УУ60/2020/92/2
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The Ukrainian biochemical journal [Текст]. - Выходит раз в два месяца
2020г. Т. 92 № 2

Содержание:
Vari, S. G. Homo scientist can lead HOMO SAPIENS to the next evolutionary stage, to HOMO ingenious / S. G. Vari. - P.5-6
Korolyuk, O. Ya. Hypertriclyceridemia is associated with long-term risk of cardiovascular events and specific comorbidity in very high-risk hypertensive patients / O. Ya. Korolyuk, O. M. Radchenko. - P.8-19
Bozhenko, M. Freezing influences, the exposure of IgG glycans in sera from multiple sclerosis patients / M. Bozhenko [et al.]. - P.21-31
Другие авторы: Boichuk M., Bila G., Nehrych T., Bilyy R.
Nasadyuk, C. M. Lectinocytochemical study of rat stomach mucosa under the conditions of cyclooxygenase-1/-2 blockage and pretreatment with H-GLU-ASP-GLY-OH / C. M. Nasadyuk [et al.]. - P.33-43
Другие авторы: Sogomonyan E. A., Yashchenko A. M., Sklyarov A. Y.
Onopchenko, O. V. The effect of N-stearoylethanolamine on the lipid composition of the rat testes and testosterone level during the early stages of streptozotocin-induced diabetes / O. V. Onopchenko [et al.]. - P.45-59
Другие авторы: Horid’ko T. M., Kosiakova H. V., Berdyshev A. G., Klimashevsky V. M., Hula N. M.
Tkachenko, O. S. Protective action of N-stearoylethanolamine on blood coagulation and arterial changes in spontaneously hypertensive rats fed cholesterol-rich diet / O. S. Tkachenko [et al.]. - P.60-71
Другие авторы: Hydz Ie. A., Kosiakova H. V., Klymenko P. P., Stohnii Y. M., Didkivskyi V. A., Chernyshenko T. M., Chernyshenko V. O., Platonova T. M.
Stohnii, Y. M. Aggregation of platelets, proliferation of endothelial cells and motility of cancer cells are mediated by the Bβ1(15)-42 residue of fibrin(ogen) / Y. M. Stohnii [et al.]. - P.72-85
Другие авторы: Ryzhykova M. V., Rebriev A. V., Kuchma M. D., Marunych R. Y., Chernyshenko V. O., Shablii V. A., Lypova N. M., Slominskyi O. Yu., Garmanchhuk L. V., Platonova T. M., Komisarenko S. V.
Revenko, O. Exogenous hydrogen sulfide for the treatment of mesenteric damage associated with fructose-induced malfunctions via inhibition of oxidative stress / O. Revenko [et al.]. - P.86-98
Другие авторы: Zaichko N., Wallace J., Zayachkivska O.
Bila, G. Novel approach for discrimination of eosinophilic granulocytes and evaluation of their surface receptors in a multicolor fluorescent histological assessment / G. Bila [et al.]. - P.99-107
Другие авторы: Schneider M., Peshkova S., Krajnik B., Besh L., Lutsyk A., Matsyura O., Bilyy R.
Kryshchyshyn, A. Synthesis and anti-leukemic activity of pyrrolidinedione-thiazolidinone hybrids / A. Kryshchyshyn [et al.]. - P.108-120
Другие авторы: Kaminskyy D., Roman O., Kralovics R., Karpenko O., Lesyk R.
Hreniukh, V. P. Effects of thiazole derivatives on intracellular structure and functions in murine lymphoma cells / V. P. Hreniukh [et al.]. - P.121-131
Другие авторы: Finiuk N. S., Shalai Ya. R., Manko B. O., Manko B. V., Ostapiuk Yu. V., Kulachkovskyy O. R., Obushak M. D., Stoika R. S., Babsky A. M.
Chaban, T. I. Development of effective anti-inflammatory drug candidates among novel thiazolopyridines / T. I. Chaban [et al.]. - P.132-139
Другие авторы: Matiychuk V. S., Ogurtsov V. V., Chaban I. G., Nektegayev I. A.
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Шифр: ЕУ12/2020/42/1
Журнал

Экспериментальная онкология [Текст]
2020г. Т. 42 № 1

Содержание:
Bubnovskaya, L. Tumor microenvironment and metabolic factors: contribution to gastric cancer / L. Bubnovskaya, D. Osinsky. - С.2-10
Gong, Y. Rapamycin-induced autophagy plays a pro-survival role by enhancing up-regulation of intracellular ferritin expression in acute lymphoblastic leukemia / Y. Gong [et al.]. - С.11-15
Другие авторы: Wu J., Yang R., Zhang L., Ma Z.
Shcherbina, V. Sensitivity of chronic lymphocytic leukemia cells to chemotherapeutic drugs ex vivo depends on expression staus of cell surface receptors / V. Shcherbina [et al.]. - С.16-24
Другие авторы: Gordiienko I., Shlapatska L., Ivanivska T., Sidorenko S.
Siromolot, A. A. Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro / A. A. Siromolot [et al.]. - С.25-30
Другие авторы: Krynina O. I., Kolybo D. V., Komisarenko S. V.
Shvachko, L. P. Aberrant expression of placental-like alkaline phosphatase in chronic myeloid leukemia cells in vitro and its modulation by vitamin E / L. P. Shvachko [et al.]. - С.31-34
Другие авторы: Zavelevich M. P., Gluzman D. F., Telegeev G. D.
Kolesnik, D. L. Metformin enhances cytotoxic action of dichloroacetate against lewis lung carcinoma cells in vitro / D. L. Kolesnik [et al.]. - С.35-39
Другие авторы: Pyaskovskaya O. N., Gorbach O., Solyanik G. I.
Sarnatskaya, V. V. Structural changes of serum albumin in response to oxidative stress caused by Walker-256 carcinosarcoma growth / V. V. Sarnatskaya [et al.]. - С.40-45
Другие авторы: Yushko L. A., Prokopenko I. V., Hudenko N. V., Maslenny V. N., Paziuk L. M., Bubnovskaya L. N., Nikolaev V. G.
Burlaka, A. A. Drag reducing polymers attenuate adverse effects of ischemia-reperfusion upon resection of liver metastases modeled by MC38 mouse colon adenocarcinoma / A. A. Burlaka [et al.]. - С.46-50
Другие авторы: Burlaka A. P., Krotevych M. S., Rudiuk T. O., Orel V. E.
Lifsics, A. Survival analysis of oropharyngeal squamous cell carcinoma patients linked to histopathology, disease stage, tumor stage, risk factors, and received therapy / A. Lifsics [et al.]. - С.51-59
Другие авторы: Rate E., Ivanova A., Tars J., Murovska M., Groma V.
Bilous, N. MYC copy number and mRNA expression in chronic lymphocytic leukemia patients exposed to ionizing radiation due to the Chornobyl NPP accident / N. Bilous [et al.]. - С.60-65
Другие авторы: Abramenko I., Chumak A., Dyagil I., Martina Z.
Fedorenko, Z. P. Incidence and long-term effects of treatment of malignant germ cell neoplasms in Ukraine / Z. P. Fedorenko [et al.]. - С.66-74
Другие авторы: Mikchailovich Yu. I., Goulak L. O., Ryzhov A. Yu., Gorokh Ye. L., Soumkina O. V.
Kozirovskis, V. High expression of GLI1 is associated with better survival in advanced SCLC / V. Kozirovskis [et al.]. - С.75-77
Другие авторы: Zandberga E., Magone M., Purkalne G., Line A., Vikmanis U.
Kearney, L. Frequency and spectrum of atypical BCR-ABL1 transcripts in chronic myeloid leukemia / L. Kearney, M. Grampe, S. E. Langabeer. - С.78-79
In commemoration of Dmitriy Vladimirovich Myasoyedov. - С.80
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Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro / A. A. Siromolot [et al.] // Experimental Oncology. - 2020. - Том 42, N 1. - P25-30

MeSH-главная:
АНТИТЕЛА НЕЙТРАЛИЗУЮЩИЕ -- ANTIBODIES, NEUTRALIZING (физиология)
НОВООБРАЗОВАНИЯ -- NEOPLASMS (патофизиология, терапия)
Аннотация: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a member of the epidermal growth factor family and has a variety of physiological and pathophysiological functions. Also, HB-EGF plays a pivotal role in progression of different tumors. So, HB-EGF seems to be a target molecule for the treatment of some cancer types. Aim: To obtain HB-EGF neutralizing polyclonal antibodies and test their anti-proliferative properties in vitro. Materials and Methods: Lab rabbits and mice were used for immunization with recombinant HB-EGF. The effect of generated polyclonal antibodies on viability and apoptosis of human epidermoid carcinoma derived A431 cell line was assessed using MTT and Annexin V-propidium iodide assays. Results: Rabbit polyclonal anti-HB-EGF serum could block binding of soluble HB-EGF to epidermal growth factor receptor/human epidermal growth factor receptor. Also, anti-HB-EGF antibodies could bind to surface of A431 cells which express abnormally high levels of membrane bound proHB-EGF and its receptor. It has been shown that immune serum with polyclonal antibodies against HB-EGF was able to block the mitogenic activation of the cells with HB-EGF and cause apoptotic cell death. Conclusion: Inhibition of HB-EGF activity with neutralizing polyclonal antibodies can effectively inhibit mitogenic activation and cause apoptosis of cancer cells with significant epidermal growth factor receptor overexpression
Доп.точки доступа:
Siromolot, A. A.
Krynina, O. I.
Kolybo, D. V.
Komisarenko, S. V.

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10.

Novel monoclonal antibody to fibrin(ogen) αC-region for detection of the earliest forms of soluble fibrin / N. E. Lugovska [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 3. - P58-70

MeSH-главная:
ФИБРИН -- FIBRIN (анализ, аналоги и дериваты, биосинтез, выделение и очистка)
Аннотация: Obtaining new monoclonal antibodies (mAbs) towards fibrin(ogen) and its fragments is an important task for studying mechanisms of blood clot formation, searching for novel antithrombotic agents and developing immunodiagnostics. The aim of the present work was to create and characterize a new mAb towards the fibrin(ogen) αС-region. We surmise that having a specific mAb towards this flexible part of the molecule will allow us to study the role of the αС-region in fibrin polymerization and also to develop an approach for detecting the earliest forms of soluble fibrin by sandwich ELISA. Using hybridoma technology we оbtained mAb 1-5A to the αC-region of fibrinogen. It was characterized using several variations of ELISA and Western blot. Application of specific proteases together with MALDI-TOF analysis allowed us to localize its epitope that is located in fragment 537-595 of the Aα-chain of fibrin(ogen). МAb 1-5A can be used as a detecting tag-antibody in sandwich ELISA for the quantification of the earliest forms of soluble fibrin which are uncleaved by plasmin and preserved C-terminal portions of αC-regions. These earliest forms of soluble fibrin are direct evidence of blood coagulation system activation, thrombin generation and the danger of intravascular thrombus formation. Their determination will provide additional, more accurate information about the state of the blood coagulation system and the risk of blood clotting, which is very important for the timely and correct selection of adequate antithrombotic therapy. MAb 1-5A effectively binds the αC-containing molecules of fibrinogen and fibrin in blood plasma. It also can be used for studying protein-protein and protein-cellular interactions of the αC-regions of fibrin(ogen)
Доп.точки доступа:
Lugovska, N. E.
Kolesnikova, I. M.
Stohnii, Ye. M.
Chernyshenko, V. O.
Rebriev, A. V.
Kostiuchenko, O. P.
Gogolinska, G. K.
Dziubliuk, N. A.
Varbanets, L. D.
Platonova, T. M.
Komisarenko, S. V.

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