Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Komisarenko S. V., Romaniuk S. I.
Заглавие : Prospects of genome editing using CRISPR/CAS or how to master genetic scissors. Nobel Prize in chemistry 2020
Место публикации : The Ukrainian biochemical journal. - Київ, 2021. - Vol. 93, № 1. - С. 113-128 (Шифр УУ60/2021/93/1)
MeSH-главная: ХИМИЯ -- CHEMISTRY
МОЛЕКУЛЯРНАЯ БИОЛОГИЯ -- MOLECULAR BIOLOGY
ГЕННАЯ ИНЖЕНЕРИЯ -- GENETIC ENGINEERING
НОБЕЛЕВСКАЯ ПРЕМИЯ -- NOBEL PRIZE
Аннотация: The Nobel Prize in Chemistry 2020 was awarded to two researchers in the field of molecular biology – the French Emmanuelle Charpentier, who now heads the Max Planck Unit for the Science of Pathogens in Berlin, and the American Jennifer Doudna from the University of California, Berkeley – for the ‘development of genome editing method’. A press release of the Nobel Committee states that the winners discovered one of the most powerful genetic technology tools – CRISPR/Cas9, or so-called ‘genetic scissors’. This method has contributed to many important results of basic research. In particular, plant researchers have managed to create crops resistant to mold, pests and drought. As for medicine, clinical trials of new cancer treatment techniques are underway, and a dream of curing hereditary diseases is about to become a reality. Genetic scissors have brought the life sciences to a new stage of development and greatly contributed to the benefit of mankindНобелівську премію з хімії у 2020 р. присуджено двом дослідницям у галузі молекулярної біології – француженці Еммануель Шарпантьє (Emmanuelle Charpentier), яка нині очолює Відділення наук про патогени при Товаристві Макса Планка в Берліні, та американці Дженніфер Дудні (Jennifer Doudna) з Каліфорнійського університету в Берклі – за «розвиток методу редагування геному». У пресрелізі Нобелівського комітету зазначено, що лауреатки відкрили один з найпотужніших інструментів генної технології – CRISPR/Cas9 – або так звані «генетичні ножиці». Цей метод сприяв отриманню у фундаментальних дослідженнях багатьох важливих результатів. Зокрема, дослідники рослин змогли створити культури, стійкі до цвілі, шкідників та посухи. У медицині тривають клінічні випробування нових методів лікування раку, а мрія про те, щоб вилікувати спадкові захворювання, ось-ось стане реальністю. «Генетичні ножиці» вивели науки про життя на новий етап розвитку і дають людству величезну користь
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Заглавие журнала :The Ukrainian biochemical journal -2021г. т.93,N 1
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Zaloilo I. Coronavirus viroporins: structure and function/ I. Zaloilo [et al.] (стр.5-17)
Meftahi G. H. A vicious circle between oxidative stress and cytokine storm in acute respiratory distress syndrome pathogenesis at Covid-19 infection/ G. H. Meftahi [et al.] (стр.18-29)
Kirana A. N. Protein intake and loss of proteostasis in the eldery/ A. N. Kirana, E. Prafiantini, N. S. Hardiany (стр.30-39)
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Grabovsky S. S. Male and female rats differ in homeostatic shifts during pre-slaughter fear stress/ S. S. Grabovsky [et al.] (стр.68-74)
Smirnov O. E. Organo-specific accumulation of phenolic compounds in a buckwheat seedlings under alum-acid stressinium/ O. E. Smirnov [et al.] (стр.75-81)
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Komisarenko S. V. Prospects of genome editing using CRISPR/CAS or how to master genetic scissors. Nobel Prize in Chemistry 2020/ S. V. Komisarenko, S. I. Romaniuk (стр.113-128)
Матишевська О. П. Відкриття механізмів біологічного синтезу нуклеїнових кислот: нобелівські лауреати 1959 р. С. Очоа і А. Корнберг/ О. П. Матишевська, В. М. Данилова, С. В. Комісаренко (стр.129-138)
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Danylova T. V., Komisarenko S. V.
Заглавие : Double Nobel prize winner: Frederick Sanger - the father of genomics
Место публикации : The Ukrainian biochemical journal. - Київ, 2021. - Vol. 93, № 2. - С. 116-122 (Шифр УУ60/2021/93/2)
MeSH-главная: НОБЕЛЕВСКАЯ ПРЕМИЯ -- NOBEL PRIZE
Аннотация: This paper aims to outline briefly the main stages of Frederick Sanger’s scientific activity – the only person to have won two Nobel Prizes in Chemistry (1958, 1980). His work on the structure of proteins, especially that of insulin, and the determination of base sequences in nucleic acids made an immense impact on the development of biochemistry and especially on the development of a new scientific field – molecular biology. His methods for determining the primary structure of proteins and nucleic acids helped biochemists and molecular biologists to determine the structure of many proteins and nucleic acids and laid the basis for genetic engineering
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Заглавие журнала :The Ukrainian biochemical journal -2021г. т.93,N 2
Интересные статьи :
Vari S. G. Inflammation is the common mechanism of diseases (CMD) in COVID-19 disease during pregnancy and in gestational diabetes mellitus/ S. G. Vari (стр.5)
Kobylinska L. In silico identification and biochemical validation of plausible molecular targets of 4-thiazolidinone derivative les-3833 as a potential anticancer agent/ L. Kobylinska [et al.] (стр.7-23)
Shevchuk O. O. Locally delivered lovastatin-containing chitosan nanoparticles promote bone regeneration in rats/ O. O. Shevchuk, Ya. V. Panasiuk, M. M. Korda (стр.24-38)
Finiuk N. S. Effect of a novel thiazole derivative and its complex with a polymeric carrier on stability of DNA in human breast cancer cells/ N. S. Finiuk [et al.] (стр.39-52)
Poshyvak O. B. Oxidative stress suppression contributes to antiseizure action of axitinib and rapamycin in pentylenetetrazol-induced kindling/ O. B. Poshyvak, O. R. Pinyazhko, L. S. Godlevsky (стр.53-60)
Korolova D. S. Thromboelastographic study of fibrin clot and molecular basis of maximum clot firmness/ D. S. Korolova [et al.] (стр.62-70)
Pavlyshyn H. A. The association between vitamin D status and cardiometabolic disorders in overweight and obese adolescents/ H. A. Pavlyshyn, A.-M. A. Shulha (стр.72-81)
Pavlyshyn H. A. Investigating the connections between night eating syndrome and metabolic syndrome in children/ H. A. Pavlyshyn, K. V. Kozak, K. T. Hlushko (стр.83-94)
Hlushko K. T. Clinical and immunological peculiarities of parasitic infections in children with digestive system disorders in Western Ukraine/ K. T. Hlushko, H. A. Pavlyshyn, K. V. Kozak (стр.96-105)
Kucher S. V. Inclusion of enterosorbents in anti-inflammatory therapy improve treatment effectiveness in COPD patients during exacerbations/ S. V. Kucher, O. V. Lototska (стр.107-114)
Danylova T. V. Double Nobel prize winner: Frederick Sanger - the father of genomics/ T. V. Danylova, S. V. Komisarenko (стр.116-122)
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Stohnii Y. M., Ryzhykova M. V., Rebriev A. V., Kuchma M. D., Marunych R. Y., Chernyshenko V. O., Shablii V. A., Lypova N. M., Slominskyi O. Yu., Garmanchuk L. V., Platonova T. M., Komisarenko S. V.
Заглавие : Aggregation of platelets, proliferation of endothelial cells and motility of cancer cells are mediated by the Bβ1(15)-42 residue of fibrin(ogen)
Место публикации : The Ukrainian biochemical journal. - Київ, 2020. - Vol. 92, № 2. - С. 72-85 (Шифр УУ60/2020/92/2)
MeSH-главная: ФИБРИНОГЕН -- FIBRINOGEN
НОВООБРАЗОВАНИЙ ИНВАЗИВНОСТЬ -- NEOPLASM INVASIVENESS
Аннотация: The fibrinogen molecule contains multiple binding motifs for different types of cellular receptors, acting as a molecular link between coagulation and cell adhesion. In this study we generated a truncated form of the fibrinogen molecule lacking the Bβ1-42 sequence by site-specific proteolysis and evaluated the role of the fragment in adhesive capabilities of platelets, endothelial and cancer cells. Fibrinogen with the removed Bβ1-42 sequence and fibrin without the Bβ15-42 fragment (desβ1-42 fibrinogen and desABβ15-42 fibrin) were obtained by proteolysis using the specific protease from the venom of Echis multisquamatis. The cleaved fragment was purified by HPLC and was identified using MALDI-TOF. ADP- and collagen-induced aggregation of washed platelets in the presence of fibrinogen desBβ1-42 was studied using an aggregometer. Proliferation of mice aortic endothelial cells (MAEC) and human umbilical vein endothelial cells (HUVEC) was studied using the fibrin desABβ15-42 as the scaffold. Cell viability was quantified by the MTT test (MAEC). Generation time was calculated for the estimation of proliferative activity of HUVEC. Lung cancer cell line Н1299 was used to evaluate cancer cell motility in vitro using the scratch assay. Direct comparison of cellular behavior in the presence of truncated vs native forms demonstrated attenuated cell adhesion in the presence of fibrinogen desBβ1-42 and fibrin desBβ15-42. The platelet aggregation rate was only slightly decreased in the presence of fibrinogen desBβ1-42 but resulted in 15-20% disaggregation of adhered platelets. We also observed the substantial decrease of generation time of HUVEC and inhibition of viability of MAEC cells grown on scaffolds of a desABβ15-42 matrix. Finally, desBβ1-42 modulated the motility of H1299 cells in vitro and suppressed the wound healing by 20% compared to the full-length fibrinogen. We postulate that fragment 1-42 of the BβN-domain of fibrinogen is not sufficient for platelet aggregation, however it may contribute to platelet clot formation in later stages. At the same time, this fragment may be important for establishing proper cell-to-cell contacts and cell viability of endothelial cells. Also, 1-42 amino acid fragment of the BβN-domain supported the migration of cancer cells suggesting that interactions of fibrinogen with cancer cells could be a target for anticancer therapy. The Bβ1-42 fragment of fibrinogen contributes to efficient intracellular interactions of different types of cells, including platelets, endothelial cells and cancer cells
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Заглавие журнала :The Ukrainian biochemical journal -2020г. т.92,N 3
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Chernyshenko V. Presenting thematic ’Molecular and clinical studies of hemostasis’ issue/ V. Chernyshenko, S. Komisarenko (стр.5)
Vari S. G. COVID-19 infection: disease mechanism, vascular dysfunction, immune responses, markers, multiorgan failure, treatments, and vaccination/ S. G. Vari
Medved L. Structure and function of fibrinogen BβN-Domains/ L. Medved, S. Yakovlev (стр.22-32)
Lugovskoi E. The fibrin Bβ125-135 site is involved in the lateral association of protofibrils/ E. Lugovskoi [et al.] (стр.33-45)
Kapustianenko L. G. Identification of the binding site for plasminogen kringle 5 in the α-chain of fibrin(ogen) D-fragment/ L. G. Kapustianenko [et al.] (стр.46-57)
Lugovska N. E. Novel monoclonal antibody to fibrin(ogen) αC-region for detection of the earliest forms of soluble fibrin/ N. E. Lugovska [et al.] (стр.58-70)
Mykhaloiko I. S. D-dimer as a potential predictor of thromboembolic and cardiovascular complications in patients with chronic kidney disease/ I. S. Mykhaloiko [et al.] (стр.71-76)
Panibratiuk O. A. Warfarin therapy in patients with coronary heart disease and atrial fibrillation: drug interactions and genetic sensitivity to warfarin/ O. A. Panibratiuk, O. A. Yakovleva (стр.77-85)
Chernyshenko V. O. Men of the molecules/ V. O. Chernyshenko (стр.86-90)
Григор’єва М. В. 20 років тому завершилися "перегони" секвенування геному людини/ М. В. Григор’єва ; М. В. Григор’єва, С. В. Комісаренко (стр.91-92)
Danylova T. V. Nobel prize winner erwin schrodinger: the physicist, philosopher, and godfather of molecular biology and genetics/ T. V. Danylova, S. V. Komisarenko (стр.93-100)
До 70-річчя від дня народження члена-кореспондента НАН України, професора Ростислава Стефановича Стойки (стр.101-102)
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Заглавие журнала :The Ukrainian biochemical journal -2020г. т.92,N 2
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Vari S. G. Homo scientist can lead HOMO SAPIENS to the next evolutionary stage, to HOMO ingenious/ S. G. Vari (стр.5-6)
Korolyuk O. Ya. Hypertriclyceridemia is associated with long-term risk of cardiovascular events and specific comorbidity in very high-risk hypertensive patients/ O. Ya. Korolyuk, O. M. Radchenko (стр.8-19)
Bozhenko M. Freezing influences, the exposure of IgG glycans in sera from multiple sclerosis patients/ M. Bozhenko [et al.] (стр.21-31)
Nasadyuk C. M. Lectinocytochemical study of rat stomach mucosa under the conditions of cyclooxygenase-1/-2 blockage and pretreatment with H-GLU-ASP-GLY-OH/ C. M. Nasadyuk [et al.] (стр.33-43)
Onopchenko O. V. The effect of N-stearoylethanolamine on the lipid composition of the rat testes and testosterone level during the early stages of streptozotocin-induced diabetes/ O. V. Onopchenko [et al.] (стр.45-59)
Tkachenko O. S. Protective action of N-stearoylethanolamine on blood coagulation and arterial changes in spontaneously hypertensive rats fed cholesterol-rich diet/ O. S. Tkachenko [et al.] (стр.60-71)
Stohnii Y. M. Aggregation of platelets, proliferation of endothelial cells and motility of cancer cells are mediated by the Bβ1(15)-42 residue of fibrin(ogen)/ Y. M. Stohnii [et al.] (стр.72-85)
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Заглавие журнала :Экспериментальная онкология -2020г. т.42,N 1
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Siromolot A. A. Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro/ A. A. Siromolot [et al.] (стр.25-30)
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Sarnatskaya V. V. Structural changes of serum albumin in response to oxidative stress caused by Walker-256 carcinosarcoma growth/ V. V. Sarnatskaya [et al.] (стр.40-45)
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Bilous N. MYC copy number and mRNA expression in chronic lymphocytic leukemia patients exposed to ionizing radiation due to the Chornobyl NPP accident/ N. Bilous [et al.] (стр.60-65)
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In commemoration of Dmitriy Vladimirovich Myasoyedov (стр.80)
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Siromolot A. A., Krynina O. I., Kolybo D. V., Komisarenko S. V.
Заглавие : Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro
Место публикации : Experimental Oncology. - К., 2020. - Том 42, N 1. - С. 25-30 (Шифр ЕУ12/2020/42/1)
MeSH-главная: АНТИТЕЛА НЕЙТРАЛИЗУЮЩИЕ -- ANTIBODIES, NEUTRALIZING
НОВООБРАЗОВАНИЯ -- NEOPLASMS
Аннотация: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a member of the epidermal growth factor family and has a variety of physiological and pathophysiological functions. Also, HB-EGF plays a pivotal role in progression of different tumors. So, HB-EGF seems to be a target molecule for the treatment of some cancer types. Aim: To obtain HB-EGF neutralizing polyclonal antibodies and test their anti-proliferative properties in vitro. Materials and Methods: Lab rabbits and mice were used for immunization with recombinant HB-EGF. The effect of generated polyclonal antibodies on viability and apoptosis of human epidermoid carcinoma derived A431 cell line was assessed using MTT and Annexin V-propidium iodide assays. Results: Rabbit polyclonal anti-HB-EGF serum could block binding of soluble HB-EGF to epidermal growth factor receptor/human epidermal growth factor receptor. Also, anti-HB-EGF antibodies could bind to surface of A431 cells which express abnormally high levels of membrane bound proHB-EGF and its receptor. It has been shown that immune serum with polyclonal antibodies against HB-EGF was able to block the mitogenic activation of the cells with HB-EGF and cause apoptotic cell death. Conclusion: Inhibition of HB-EGF activity with neutralizing polyclonal antibodies can effectively inhibit mitogenic activation and cause apoptosis of cancer cells with significant epidermal growth factor receptor overexpression
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lugovska N. E., Kolesnikova I. M., Stohnii Ye. M., Chernyshenko V. O., Rebriev A. V., Kostiuchenko O. P., Gogolinska G. K., Dziubliuk N. A., Varbanets L. D., Platonova T. M., Komisarenko S. V.
Заглавие : Novel monoclonal antibody to fibrin(ogen) αC-region for detection of the earliest forms of soluble fibrin
Место публикации : The Ukrainian biochemical journal. - Київ, 2020. - Vol. 92, № 3. - С. 58-70 (Шифр УУ60/2020/92/3)
MeSH-главная: ФИБРИН -- FIBRIN
Аннотация: Obtaining new monoclonal antibodies (mAbs) towards fibrin(ogen) and its fragments is an important task for studying mechanisms of blood clot formation, searching for novel antithrombotic agents and developing immunodiagnostics. The aim of the present work was to create and characterize a new mAb towards the fibrin(ogen) αС-region. We surmise that having a specific mAb towards this flexible part of the molecule will allow us to study the role of the αС-region in fibrin polymerization and also to develop an approach for detecting the earliest forms of soluble fibrin by sandwich ELISA. Using hybridoma technology we оbtained mAb 1-5A to the αC-region of fibrinogen. It was characterized using several variations of ELISA and Western blot. Application of specific proteases together with MALDI-TOF analysis allowed us to localize its epitope that is located in fragment 537-595 of the Aα-chain of fibrin(ogen). МAb 1-5A can be used as a detecting tag-antibody in sandwich ELISA for the quantification of the earliest forms of soluble fibrin which are uncleaved by plasmin and preserved C-terminal portions of αC-regions. These earliest forms of soluble fibrin are direct evidence of blood coagulation system activation, thrombin generation and the danger of intravascular thrombus formation. Their determination will provide additional, more accurate information about the state of the blood coagulation system and the risk of blood clotting, which is very important for the timely and correct selection of adequate antithrombotic therapy. MAb 1-5A effectively binds the αC-containing molecules of fibrinogen and fibrin in blood plasma. It also can be used for studying protein-protein and protein-cellular interactions of the αC-regions of fibrin(ogen)
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