Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Stohnii Y. M., Ryzhykova M. V., Rebriev A. V., Kuchma M. D., Marunych R. Y., Chernyshenko V. O., Shablii V. A., Lypova N. M., Slominskyi O. Yu., Garmanchuk L. V., Platonova T. M., Komisarenko S. V.
Заглавие : Aggregation of platelets, proliferation of endothelial cells and motility of cancer cells are mediated by the Bβ1(15)-42 residue of fibrin(ogen)
Место публикации : The Ukrainian biochemical journal. - Київ, 2020. - Vol. 92, № 2. - С. 72-85 (Шифр УУ60/2020/92/2)
MeSH-главная: ФИБРИНОГЕН -- FIBRINOGEN
НОВООБРАЗОВАНИЙ ИНВАЗИВНОСТЬ -- NEOPLASM INVASIVENESS
Аннотация: The fibrinogen molecule contains multiple binding motifs for different types of cellular receptors, acting as a molecular link between coagulation and cell adhesion. In this study we generated a truncated form of the fibrinogen molecule lacking the Bβ1-42 sequence by site-specific proteolysis and evaluated the role of the fragment in adhesive capabilities of platelets, endothelial and cancer cells. Fibrinogen with the removed Bβ1-42 sequence and fibrin without the Bβ15-42 fragment (desβ1-42 fibrinogen and desABβ15-42 fibrin) were obtained by proteolysis using the specific protease from the venom of Echis multisquamatis. The cleaved fragment was purified by HPLC and was identified using MALDI-TOF. ADP- and collagen-induced aggregation of washed platelets in the presence of fibrinogen desBβ1-42 was studied using an aggregometer. Proliferation of mice aortic endothelial cells (MAEC) and human umbilical vein endothelial cells (HUVEC) was studied using the fibrin desABβ15-42 as the scaffold. Cell viability was quantified by the MTT test (MAEC). Generation time was calculated for the estimation of proliferative activity of HUVEC. Lung cancer cell line Н1299 was used to evaluate cancer cell motility in vitro using the scratch assay. Direct comparison of cellular behavior in the presence of truncated vs native forms demonstrated attenuated cell adhesion in the presence of fibrinogen desBβ1-42 and fibrin desBβ15-42. The platelet aggregation rate was only slightly decreased in the presence of fibrinogen desBβ1-42 but resulted in 15-20% disaggregation of adhered platelets. We also observed the substantial decrease of generation time of HUVEC and inhibition of viability of MAEC cells grown on scaffolds of a desABβ15-42 matrix. Finally, desBβ1-42 modulated the motility of H1299 cells in vitro and suppressed the wound healing by 20% compared to the full-length fibrinogen. We postulate that fragment 1-42 of the BβN-domain of fibrinogen is not sufficient for platelet aggregation, however it may contribute to platelet clot formation in later stages. At the same time, this fragment may be important for establishing proper cell-to-cell contacts and cell viability of endothelial cells. Also, 1-42 amino acid fragment of the BβN-domain supported the migration of cancer cells suggesting that interactions of fibrinogen with cancer cells could be a target for anticancer therapy. The Bβ1-42 fragment of fibrinogen contributes to efficient intracellular interactions of different types of cells, including platelets, endothelial cells and cancer cells
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Siromolot A. A., Krynina O. I., Kolybo D. V., Komisarenko S. V.
Заглавие : Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro
Место публикации : Experimental Oncology. - К., 2020. - Том 42, N 1. - С. 25-30 (Шифр ЕУ12/2020/42/1)
MeSH-главная: АНТИТЕЛА НЕЙТРАЛИЗУЮЩИЕ -- ANTIBODIES, NEUTRALIZING
НОВООБРАЗОВАНИЯ -- NEOPLASMS
Аннотация: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a member of the epidermal growth factor family and has a variety of physiological and pathophysiological functions. Also, HB-EGF plays a pivotal role in progression of different tumors. So, HB-EGF seems to be a target molecule for the treatment of some cancer types. Aim: To obtain HB-EGF neutralizing polyclonal antibodies and test their anti-proliferative properties in vitro. Materials and Methods: Lab rabbits and mice were used for immunization with recombinant HB-EGF. The effect of generated polyclonal antibodies on viability and apoptosis of human epidermoid carcinoma derived A431 cell line was assessed using MTT and Annexin V-propidium iodide assays. Results: Rabbit polyclonal anti-HB-EGF serum could block binding of soluble HB-EGF to epidermal growth factor receptor/human epidermal growth factor receptor. Also, anti-HB-EGF antibodies could bind to surface of A431 cells which express abnormally high levels of membrane bound proHB-EGF and its receptor. It has been shown that immune serum with polyclonal antibodies against HB-EGF was able to block the mitogenic activation of the cells with HB-EGF and cause apoptotic cell death. Conclusion: Inhibition of HB-EGF activity with neutralizing polyclonal antibodies can effectively inhibit mitogenic activation and cause apoptosis of cancer cells with significant epidermal growth factor receptor overexpression
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Danylova T. V., Komisarenko S. V.
Заглавие : Born in Ukraine: Nobel prize Winners Ilya Mechnikov, Selman Waksman, Roald Hoffmann, and Georges Charpak
Параллельн. заглавия :Народжені в Україні лауреати Нобелівської премії: Ілля Мечников, Зельман Ваксман, Роалд Гоффман і Жорж Шарпак
Место публикации : The Ukrainian Biochemical Journal. - 2019. - Т. 91, № 3. - С. 127-137 (Шифр УУ60/2019/91/3)
Примечания : Bibliogr. at the end of the art.
MeSH-главная: ЛИЧНОСТИ ВЫДАЮЩИЕСЯ -- FAMOUS PERSONS
НОБЕЛЕВСКАЯ ПРЕМИЯ -- NOBEL PRIZE
УКРАИНА -- UKRAINE
Аннотация: Our country has not yet gained recognition from a Nobel Committee, however, some Nobel Prize winners were born in the territory, which belongs to present-day Ukraine. Among them are the father of innate cellular immunity Ilya Mechnikov; the famous microbiologist and biochemist Selman Waksman, whose studies had led to the discovery of streptomycin; the outstanding chemist, poet and playwright Roald Hoffmann, and the prominent physicist Georges Charpak who invented and developed particle detectors, in particular, the multiwire proportional chamber. This paper aims to outline briefly the main stages of their scientific activityНаша країна ще не отримала визнання від Нобелівського комітету, проте деякі лауреати Нобелівської премії народилися на території, яка належить сучасній Україні. Серед них батько клітинного вродженого імунітету Ілля Мечников; відомий мікробіолог і біохімік Зельман Ваксман, чиї дослідження привели до відкриття стрептоміцину; видатний хімік, поет і драматург Роалд Гоффман і славетний фізик Жорж Шарпак, який розробив і вдосконалив детектори елементарних частинок, зокрема багатодротову пропорційну камеру. У цій статті представлено короткий огляд основних етапів їхньої наукової діяльності
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Grigorieva M. V., Danilova V. M., Komisarenko S. V.
Заглавие : Brownian motion, electrophoresis, chromatography, and macromolecular chemistry: how it all unites Nobel laureates of the first half of the 20th century – T. Svedberg, A. Tiselius, R. Synge and H. Staudinger
Параллельн. заглавия :Броунівський рух, електрофорез, хроматографія та макромолекулярна хімія: як все це об’єднує нобелівських лауреатів першої половини ХХ ст. – Т. Сведберга, А. Тізеліуса, Р. Сінга і Г. Штаудінгера
Место публикации : The Ukrainian Biochemical Journal. - 2019. - Т. 91, № 5. - С. 70-79 (Шифр УУ60/2019/91/5)
Примечания : Bibliogr. at the end of the art.
MeSH-главная: НОБЕЛЕВСКАЯ ПРЕМИЯ -- NOBEL PRIZE
БИОМЕДИЦИНСКИЕ ИССЛЕДОВАНИЯ -- BIOMEDICAL RESEARCH
ХРОМАТОГРАФИЯ -- CHROMATOGRAPHY
ЭЛЕКТРОФОРЕЗ -- ELECTROPHORESIS
ЛИЧНОСТИ ВЫДАЮЩИЕСЯ -- FAMOUS PERSONS
ЭЛЕМЕНТАРНЫХ ЧАСТИЦ ВЗАИМОДЕЙСТВИЕ -- ELEMENTARY PARTICLE INTERACTIONS
Аннотация: It is hard to imagine how chemistry, biology and medicine would develop without such techniques as ultracentrifugation, electrophoresis and chromatography. At present innovative hi-tech laboratory ultracentrifuges are widely used in various fields of fundamental science and practice, including colloid chemistry, biochemical analysis, virology, clinical diagnostics, pharmacy, nanotechnology, to name a few. Electrophoresis enables to detect protein abnormalities with high probability, and, therefore, has wide application for the diagnosis of infectious-inflammatory diseases, genetic and immune disorders, malignant tumors and others. Chromatography is widely used for biochemical research and analytical detection and control of drugs and food. But where did these methods, which have had a significant impact on the development of various fields of scientific and economic activity, come from? Who were the pioneers in this field and whose work influenced the formation of the next generation of researchers? These are the questions we address in this articleВажко уявити як би розвивалися хімія, біологія і медицина без таких аналітичних методів дослідження як ультрацентрифугування, електрофорез та хроматографія. Наразі інноваційні високотехнологічні лабораторні ультрацентрифуги широко застосовуються в самих різних напрямах наукової і практичної діяльності, зокрема в колоїдній хімії, біохімічному аналізі, вірусології, клінічній діагностиці, фармації, нанотехнології та ін. Електрофорез із високою ймовірністю дозволяє виявити протеїнові аномалії, а тому широко використовується в багатьох медичних центрах різних країн для діагностики інфекційно-запальних захворювань, генетичних та імунних порушень, злоякісних пухлин тощо. Хроматографія широко застосовується для біохімічних досліджень, контролю лікарських препаратів, харчових продуктів. Але як виникли ці методи досліджень, які мали величезний вплив на розвиток не тільки різних галузей науки, але багатьох напрямів людської діяльності? Хто були ті особистості, роботи яких стали піонерськими і вплинули на дослідження наступних поколінь науковців? У цій статті ми намагаємося відповісти на ці запитання
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Komisarenko S. V., Kosterin S. O., Lugovskoy E. V., Kalchenko V. I.
Заглавие : Calixarene methylene bisphosphonic acids as promising effectors of biochemical processes
Место публикации : Український біохімічний журнал: Науч.-теорет.журн./ НАН України, Ін-т біохімії ім. О.В.Палладіна. - К., 2013. - Т. 85, № 6. - С. 106-128. - ISSN 0201-8470 (Шифр УУ1/2013/85/6). - ISSN 0201-8470
Примечания : Библиогр. в конце ст.
MeSH-главная: КАЛИКСАРЕНЫ -- CALIXARENES
АТФАЗА-CA(2+) MG(2+) -- CA(2+) MG(2+)-ATPASE
ФИБРИН -- FIBRIN
ПОЛИМЕРИЗАЦИЯ -- POLYMERIZATION
ФИБРИНОГЕН -- FIBRINOGEN
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chudina T. O., Labintsev A. J., Romaniuk S. I., Kolybo D. V., Komisarenko S. V.
Заглавие : Changes in proHB-EGF expression after functional activation of the immune system cells
Место публикации : The Ukrainian Biochemical Journal. - Київ, 2017. - Том 89, N 6. - С. 31-38 (Шифр УУ60/2017/89/6)
MeSH-главная: ИММУНОГЕНЕТИЧЕСКИЕ ПРОЦЕССЫ -- IMMUNOGENETIC PROCESSES
ЛИПОПОЛИСАХАРИДЫ -- LIPOPOLYSACCHARIDES
Аннотация: The level of proHB-EGF expression on J774, Raji, KG-1 cells derived from different types of human and mouse immune system cells under the standard in vitro culture conditions and during functional activation of these cells was investigated. Changes in the proHB-EGF expression on the cell surface were found to depend on the density of cell population, the content of fetal bovine serum in the culture medium, the effect of mitogenic factors – bacterial lipopolysaccharide, an inactive full-size form of diphtheria toxin (CRM197) and recombinant soluble HB-EGF – rsHB-EGF. The results obtained are important for the understanding of the functional role of proHB-EGF receptor on the surface of macrophage-like cells and B lymphocytes and indicate the involvement of this receptor in immune response regulation in an organism
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Danylova T. V., Komisarenko S. V.
Заглавие : Double Nobel prize winner: Frederick Sanger - the father of genomics
Место публикации : The Ukrainian biochemical journal. - Київ, 2021. - Vol. 93, № 2. - С. 116-122 (Шифр УУ60/2021/93/2)
MeSH-главная: НОБЕЛЕВСКАЯ ПРЕМИЯ -- NOBEL PRIZE
Аннотация: This paper aims to outline briefly the main stages of Frederick Sanger’s scientific activity – the only person to have won two Nobel Prizes in Chemistry (1958, 1980). His work on the structure of proteins, especially that of insulin, and the determination of base sequences in nucleic acids made an immense impact on the development of biochemistry and especially on the development of a new scientific field – molecular biology. His methods for determining the primary structure of proteins and nucleic acids helped biochemists and molecular biologists to determine the structure of many proteins and nucleic acids and laid the basis for genetic engineering
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Labyntsev А. J., Kolybo D. V., Yurchenko E. S., Korotkevych N. V., Komisarenko S. V.
Заглавие : Effect of the t-domain on intracellular transport of diphtheria toxin
Место публикации : Український біохімічний журнал: Науч.-теорет.журн./ НАН України, Ін-т біохімії ім. О.В.Палладіна. - К., 2014. - Т. 86, № 3. - С. 77-87. - ISSN 0201-8470 (Шифр УУ1/2014/86/3). - ISSN 0201-8470
Примечания : Библиогр. в конце ст.
MeSH-главная: ДИФТЕРИЙНЫЙ ТОКСИН -- DIPHTHERIA TOXIN
ЭНДОЦИТОЗ -- ENDOCYTOSIS
МИКРОСКОПИЯ КОНФОКАЛЬНАЯ -- MICROSCOPY, CONFOCAL
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bobrovnik S. A., Demchenko M. O., Komisarenko S. V.
Заглавие : Effect of trifluoroethanol on antibody reactivity against corresponding and nonrealated antigens
Место публикации : The Ukrainian Biochemical Journal. - Київ, 2018. - Том 90, N 4. - С. 80-89 (Шифр УУ60/2018/90/4)
MeSH-главная: ТРИФТОРЭТАНОЛ -- TRIFLUOROETHANOL
ИММУНОГЛОБУЛИНЫ -- IMMUNOGLOBULINS
АНТИГЕН-АНТИТЕЛО РЕАКЦИИ -- ANTIGEN-ANTIBODY REACTIONS
Аннотация: The ability of antibodies to switch between specific and nonspecific recognition of antigens under various factors is the key issue. Here we demonstrate that 2,2,2-trifluoroethanol (TFE) is one of these factors influencing the ability of monoclonal antibodies to react specifically with corresponding antigen (ovalbumin) and transforming them into polyreactive immunoglobulins (PRIGs) that are strong but nonspecific binders with various antigens. Such switching of antibody reactivity is nonlinear and even nonmonotonous function of TFE concentration and depends strongly on incubation time and temperature. At room temperatures (25 °C) the specific antibodies under 30% TFE action are transformed into PRIGs. However, at 0 °C the variation of antibody reactivity is complicated. TFE is known as the alcohol with one of the strongest proton-donor abilities in hydrogen bonding and its effect is probably in binding to specific sites that switch the antibody recognition ability
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chernyshenko V. O., Savchuk O. V., Cherenok S. O., Silenko O. M., Negelia A. O., Kasatkina L. O., Pirogova L. V., Didkivskyi V. A., Yusova O. I., Kalchenko V. I., Garmanchuk L. V., Grinenko T. V., Lugovskoy E. V., Komisarenko S. V.
Заглавие : Haemostasis modulation by calix[4]arene methylenebisphosphonic acid C-145 and its sulfur-containing analogue
Место публикации : The Ukrainian Biochemical Journal. - Київ, 2018. - Том 90, N 6. - С. 21-30 (Шифр УУ60/2018/90/6)
MeSH-главная: ГЕМОСТАЗ -- HEMOSTASIS
ФИБРИНОЛИЗ -- FIBRINOLYSIS
СВЕРТЫВАНИЕ КРОВИ -- BLOOD COAGULATION
ФИБРИНОЛИТИЧЕСКИЕ СРЕДСТВА -- FIBRINOLYTIC AGENTS
Аннотация: C-145 (octasodium salt of calix[4]arene-tetra-methylenebisphosphonic acid) was previously considered as specific anti-сoagulant agent that affects fibrin polymerization and does not notably influence other parameters of coagulation system. C-145S (octasodium salt of thiacalix[4]arene-tetra-methylenebisphosphonic acid) possessing wider hydrophobic hole was expected to be more effective antithrombotic agent than C-145. The aim of present work was to compare the action of both organic compounds on fibrin polymerization, fibrinolysis, platelets and endothelial cells. The change of turbidity during fibrin clot formation induced by APTT-reagent and digestion induced by tPA was estimated. Turbidity study was used for the estimation of polymeric fibrin hydrolysis by plasmin in the presence of thiacalix[4]arene C-145S and calix[4]arene C-145. Effects of thiacalix[4]arene C-145S and calix[4]arene C-145 on the activation of Glu-plasminogen by streptokinase were studied using chromogenic substrate S2251. Platelet aggregation study was performed using aggregometry. Stimulated Ca2+ efflux from endoplasmic reticulum and cytoplasm were determined using specific Ca2+-sensitive probes targeted to endoplasmic reticulum (Mag-Fluo-4) and cytoplasm (FURA-2) by spectrofluorimetry. Both C-145 and C-145S decreased the final turbidity of clot and prolonged clot lysis time in blood plasma in comparison to control value. C-145 was shown to be the more effective fibrinolysis inhibitor when studied in model system of polymerized fibrin desAB. C-145S but not C-145 induced concentration changes of Ca2+ in cytoplasm of resting platelets and significantly inhibited (up to 30%) Ca2+ efflux from endoplasmic reticulum of platelets activated by ADP. Both C-145 and C-145S stimulated the proliferation of endothelial cells of PAE cell line. The effect of C-145S was more prominent. In conclusion, calix[4]arene C 145S proved to be the more potent inhibitor of fibrin polymerization in comparison to C-145, which suggested earlier as anticoagulant agent. C-145S proved to have much more outlined inhibitory action on Ca2+-signaling in platelets and stimulatory effect on endothelial cells proliferation. Thus C-145 remained the most prospective molecular platform for the development of antithrombotic agent
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