Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

Главная

Упрощенный режим

Видео-инструкция

Описание

Базы данных

Периодические издания- результаты поиска

Вид поиска

Книги

Периодические издания

Область поиска

Найдено в других БД:

Книги (2)

Формат представления найденных документов:
полный	информационный	краткий

Отсортировать найденные документы по:
автору	заглавию	году издания	типу документа

Поисковый запрос: (<.>A=Kuchma, M.$<.>)

Общее количество найденных документов : 2
Показаны документы с 1 по 2

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Stohnii Y. M., Ryzhykova M. V., Rebriev A. V., Kuchma M. D., Marunych R. Y., Chernyshenko V. O., Shablii V. A., Lypova N. M., Slominskyi O. Yu., Garmanchuk L. V., Platonova T. M., Komisarenko S. V.
Заглавие : Aggregation of platelets, proliferation of endothelial cells and motility of cancer cells are mediated by the Bβ1(15)-42 residue of fibrin(ogen)
Место публикации : The Ukrainian biochemical journal. - Київ, 2020. - Vol. 92, № 2. - С. 72-85 (Шифр УУ60/2020/92/2)
MeSH-главная: ФИБРИНОГЕН -- FIBRINOGEN
НОВООБРАЗОВАНИЙ ИНВАЗИВНОСТЬ -- NEOPLASM INVASIVENESS
Аннотация: The fibrinogen molecule contains multiple binding motifs for different types of cellular receptors, acting as a molecular link between coagulation and cell adhesion. In this study we generated a truncated form of the fibrinogen molecule lacking the Bβ1-42 sequence by site-specific proteolysis and evaluated the role of the fragment in adhesive capabilities of platelets, endothelial and cancer cells. Fibrinogen with the removed Bβ1-42 sequence and fibrin without the Bβ15-42 fragment (desβ1-42 fibrinogen and desABβ15-42 fibrin) were obtained by proteolysis using the specific protease from the venom of Echis multisquamatis. The cleaved fragment was purified by HPLC and was identified using MALDI-TOF. ADP- and collagen-induced aggregation of washed platelets in the presence of fibrinogen desBβ1-42 was studied using an aggregometer. Proliferation of mice aortic endothelial cells (MAEC) and human umbilical vein endothelial cells (HUVEC) was studied using the fibrin desABβ15-42 as the scaffold. Cell viability was quantified by the MTT test (MAEC). Generation time was calculated for the estimation of proliferative activity of HUVEC. Lung cancer cell line Н1299 was used to evaluate cancer cell motility in vitro using the scratch assay. Direct comparison of cellular behavior in the presence of truncated vs native forms demonstrated attenuated cell adhesion in the presence of fibrinogen desBβ1-42 and fibrin desBβ15-42. The platelet aggregation rate was only slightly decreased in the presence of fibrinogen desBβ1-42 but resulted in 15-20% disaggregation of adhered platelets. We also observed the substantial decrease of generation time of HUVEC and inhibition of viability of MAEC cells grown on scaffolds of a desABβ15-42 matrix. Finally, desBβ1-42 modulated the motility of H1299 cells in vitro and suppressed the wound healing by 20% compared to the full-length fibrinogen. We postulate that fragment 1-42 of the BβN-domain of fibrinogen is not sufficient for platelet aggregation, however it may contribute to platelet clot formation in later stages. At the same time, this fragment may be important for establishing proper cell-to-cell contacts and cell viability of endothelial cells. Also, 1-42 amino acid fragment of the BβN-domain supported the migration of cancer cells suggesting that interactions of fibrinogen with cancer cells could be a target for anticancer therapy. The Bβ1-42 fragment of fibrinogen contributes to efficient intracellular interactions of different types of cells, including platelets, endothelial cells and cancer cells
Найти похожие

Заглавие журнала :The Ukrainian biochemical journal -2020г. т.92,N 2
Интересные статьи :
Vari S. G. Homo scientist can lead HOMO SAPIENS to the next evolutionary stage, to HOMO ingenious/ S. G. Vari (стр.5-6)
Korolyuk O. Ya. Hypertriclyceridemia is associated with long-term risk of cardiovascular events and specific comorbidity in very high-risk hypertensive patients/ O. Ya. Korolyuk, O. M. Radchenko (стр.8-19)
Bozhenko M. Freezing influences, the exposure of IgG glycans in sera from multiple sclerosis patients/ M. Bozhenko [et al.] (стр.21-31)
Nasadyuk C. M. Lectinocytochemical study of rat stomach mucosa under the conditions of cyclooxygenase-1/-2 blockage and pretreatment with H-GLU-ASP-GLY-OH/ C. M. Nasadyuk [et al.] (стр.33-43)
Onopchenko O. V. The effect of N-stearoylethanolamine on the lipid composition of the rat testes and testosterone level during the early stages of streptozotocin-induced diabetes/ O. V. Onopchenko [et al.] (стр.45-59)
Tkachenko O. S. Protective action of N-stearoylethanolamine on blood coagulation and arterial changes in spontaneously hypertensive rats fed cholesterol-rich diet/ O. S. Tkachenko [et al.] (стр.60-71)
Stohnii Y. M. Aggregation of platelets, proliferation of endothelial cells and motility of cancer cells are mediated by the Bβ1(15)-42 residue of fibrin(ogen)/ Y. M. Stohnii [et al.] (стр.72-85)
Revenko O. Exogenous hydrogen sulfide for the treatment of mesenteric damage associated with fructose-induced malfunctions via inhibition of oxidative stress/ O. Revenko [et al.] (стр.86-98)
Bila G. Novel approach for discrimination of eosinophilic granulocytes and evaluation of their surface receptors in a multicolor fluorescent histological assessment/ G. Bila [et al.] (стр.99-107)
Kryshchyshyn A. Synthesis and anti-leukemic activity of pyrrolidinedione-thiazolidinone hybrids/ A. Kryshchyshyn [et al.] (стр.108-120)
Hreniukh V. P. Effects of thiazole derivatives on intracellular structure and functions in murine lymphoma cells/ V. P. Hreniukh [et al.] (стр.121-131)
Chaban T. I. Development of effective anti-inflammatory drug candidates among novel thiazolopyridines/ T. I. Chaban [et al.] (стр.132-139)
Интересные статьи :
Найти похожие

Тематический навигатор

Статистика обращений

© Международная Ассоциация пользователей и разработчиков электронных библиотек и новых информационных технологий
(Ассоциация ЭБНИТ)