Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Tissue-engineered bone for treatment of combat related limb injuries [Текст] / R. G. Vasyliev [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 191-196. - Bibliogr. at the end of the art.

MeSH-главная:
СЛОЖНЫЕ ТКАНЕВЫЕ АЛЛОТРАНСПЛАНТАТЫ -- COMPOSITE TISSUE ALLOGRAFTS (трансплантация)
КОСТНОГО МОЗГА ТРАНСПЛАНТАЦИЯ -- BONE MARROW TRANSPLANTATION (методы, тенденции)
ВОЕННОСЛУЖАЩИЕ -- MILITARY PERSONNEL
ПЕРЕЛОМЫ КОСТИ -- FRACTURES, BONE (патофизиология, этиология)
СТВОЛОВЫХ КЛЕТОК МЕЗЕНХИМНЫХ ТРАНСПЛАНТАЦИЯ -- MESENCHYMAL STEM CELL TRANSPLANTATION (использование, методы, тенденции)
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS
Аннотация: Based on our preliminary positive clinical results with use of cultured bone marrow-derived multipotent mesenchymal stem/stromal cells in traumatology, our aim was to develop living three-dimensional tissue-engineered bone equivalent transplantation technology for restoration of critical sized bone defects caused by combat related high energy trauma. Materials and Methods: To fabricate bone equivalent we used devitalized allogeneic bone scaffolds (blocks and chips) seeded with cultured autologous cells: bone marrow-derived multipotent mesenchymal stem/stromal cells in mix with periosteal progenitor cells and endothelial progenitor cells. Quality/identity of cell cultures was assured by donor and cell culture infection screening (immunofluorescence assay, polymerase chain reaction), flow cytometry (cell phenotype), karyotyping (GTG banding), functional assays (colony forming units analysis, multilineage differentiation assay). Bone defect treatment with bone equivalent application was fully completed in 39 combat-injured with 42 defects. New bone formation was assessed by the radiographic examination. Results: Casualties were included in a treatment program an average of 10.1 months after injury, provided the ineffectiveness of conventional surgery methods. All cell type cultures had a normal karyotype and appropriate phenotype, differentiation potential and functional properties, ~30% colony forming units frequency and hadn’t any signs of cell senescence. The fluorescein diacetate/propidium iodide combined staining and histology analysis of graft samples before transplantation showed their regular seeding with viable cells. Pathomorphological analysis of bone equivalent specimens 3–6 months post-op revealed the active remodeling processes and immature bone tissue formation. Bone defect restoration was observed 5–6 months post-op. Conclusion: The developed biotechnology of living three-dimensional tissue-engineered bone equivalent transplantation with overall effectiveness 90.4% allows restoring the bone integrity, forming new bone tissue in a site of bone defect, and significantly reducing the rehabilitation period of a patient
Доп.точки доступа:
Vasyliev, R. G.
Oksymets, V. M.
Rodnichenko, A. E.
Zlatska, A. V.
Gubar, O. S.
Gordiienko, I. M.
Zubov, D. O.

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Postnatal extra-embryonic tissues as a source of multiple cell types for regenerative medicine applications [Текст] / O. S. Gubar [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 186-190. - Bibliogr. at the end of the art.

MeSH-главная:
СТРОМАЛЬНЫЕ КЛЕТКИ МЕЗЕНХИМНЫЕ -- MESENCHYMAL STROMAL CELLS (ультраструктура)
ХОРИОНАЛЬНЫЕ ВОРСИНКИ -- CHORIONIC VILLI (эмбриология)
ПЛОДА КРОВЬ -- FETAL BLOOD
АЛЛОТРАНСПЛАНТАЦИЯ -- TRANSPLANTATION, HOMOLOGOUS (использование, методы, тенденции)
ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
Аннотация: We aimed to isolate and characterize the cell types which could be obtained from postnatal extra-embryonic tissues. Materials and Methods: Fresh tissues (no more than 12 h after delivery) were used for enzymatic or explants methods of cell isolation. Obtained cultures were further maintained at 5% oxygen. At P3 cell phenotype was assessed by fluorescence-activated cell sorting, population doubling time was calculated and the multilineage differentiation assay was performed. Results: We have isolated multiple cell types from postnatal tissues. Namely, placental mesenchymal stromal cells from placenta chorionic disc, chorionic membrane mesenchymal stromal cells (ChM-MSC) from free chorionic membrane, umbilical cord MSC (UC-MSC) from whole umbilical cord, human umbilical vein endothelial cells (HUVEC) from umbilical vein, amniotic epithelial cells (AEC) and amniotic MSC (AMSC) from amniotic membrane. All isolated cell types displayed high proliferation rate together with the typical MSC phenotype: CD73⁺CD90⁺CD105⁺CD146⁺CD166⁺CD34⁻CD45⁻HLA⁻DR⁻. HUVEC constitutively expressed key markers CD31 and CD309. Most MSC and AEC were capable of osteogenic and adipogenic differentiation. Conclusion: We have shown that a wide variety of cell types can be easily isolated from extra-embryonic tissues and expanded ex vivo for regenerative medicine applications. These cells possess typical MSC properties and can be considered an alternative for adult MSC obtained from bone marrow or fat, especially for allogeneic use
Доп.точки доступа:
Gubar, O. S.
Rodnichenko, A. E.
Vasyliev, R. G.
Zlatska, A. V.
Zubov, D. O.

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Normal and cancer stem cells: discovery diagnosis and therapy international scientific conference [Текст] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 234-256

MeSH-главная:
КЛИНИЧЕСКАЯ КОНФЕРЕНЦИЯ -- CLINICAL CONFERENCE
ОНКОЛОГИЯ МЕДИЦИНСКАЯ -- MEDICAL ONCOLOGY (организация и управление, статистика, тенденции)
РАДИАЦИОННАЯ ОНКОЛОГИЯ -- RADIATION ONCOLOGY (методы, организация и управление, тенденции)
КРАТКИЙ ОБЗОР -- OUTLINES
Аннотация: R. E. Kavetsky Institute of Experimental Pathology Oncology and Radiobiology National Academy of Sciences of Ukraine Kyiv October 5–6 2017
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Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications [Текст] / R. G. Vasyliev [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 171-180. - Bibliogr. at the end of the art.

MeSH-главная:
СТВОЛОВЫХ КЛЕТОК ИССЛЕДОВАНИЕ -- STEM CELL RESEARCH
СТРОМАЛЬНЫЕ КЛЕТКИ МЕЗЕНХИМНЫЕ -- MESENCHYMAL STROMAL CELLS (физиология)
НЕРВНЫЙ ГРЕБЕШОК -- NEURAL CREST (ультраструктура, физиология)
ВОЛОСЯНОЙ ФОЛЛИКУЛ -- HAIR FOLLICLE (анатомия и гистология, ультраструктура)
ЧЕЛОВЕК -- HUMANS (анатомия и гистология)
ЦИТОФЛЮОРОМЕТРИЯ -- FLOW CYTOMETRY (использование, тенденции)
МОРФОЛОГИЧЕСКИЕ И МИКРОСКОПИЧЕСКИЕ ПОКАЗАТЕЛИ -- MORPHOLOGICAL AND MICROSCOPIC FINDINGS
ФЕНОТИП -- PHENOTYPE
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS
Аннотация: The purpose of this work was to obtain, multiply and characterize the adult neural crest-derived multipotent stem cells from human hair follicle for their further clinical use. Materials and Methods: Adult neural crest-derived multipotent stem cells were obtained from human hair follicle by explant method and were expanded at large-scale up to a clinically significant number. The resulted cell cultures were examined by flow cytometry and immunocytochemical analysis. Their clonogenic potential, ability to self-renewal and directed multilineage differentiation were also investigated. Results: Cell cultures were obtained from explants of adult human hair follicles. Resulted cells according to morphological, phenotypic and functional criteria satisfied the definition of neural crest-derived multipotent stem cells. They had the phenotype Sox2⁺Sox10⁺Nestin⁺CD73⁺CD90⁺CD105⁺CD140a⁺CD 140b⁺CD146⁺CD166⁺CD271⁺CD349⁺ CD34⁻CD45⁻CD56⁻HLA⁻DR⁻, showed high clonogenic potential, ability to self-renewal and directed differentiation into the main derivatives of the neural crest: neurons, Schwann cells, adipocytes and osteoblasts. Conclusion: The possibility of a large-scale expansion of adult neural crest-derived multipotent stem cells up to 40–200·106 cells from minimal number of hair follicles with retention of their phenotype and functional properties are the significant step towards their translation into the clinical practice
Доп.точки доступа:
Vasyliev, R. G.
Rodnichenko, A. E.
Gubar, O. S.
Zlatska, A. V.
Gordiienko, I. M.
Novikova, S. N.
Zubov, D. O.

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Kalynychenko, T. O.
Umbilical cord blood banking in the worldwide hematopoietic stem cell transplantation system: perspectives for Ukraine [Текст] / T. O. Kalynychenko // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 164-170. - Bibliogr. at the end of the art.

MeSH-главная:
БЕЛКИ КРОВИ -- BLOOD PROTEINS (анализ, снабжение и распределение)
ПЛОДА КРОВЬ -- FETAL BLOOD (трансплантация)
КРОВЕТВОРНЫЕ СТВОЛОВЫЕ КЛЕТКИ -- HEMATOPOIETIC STEM CELLS
ТЕХНОЛОГИЙ РАСПРОСТРАНЕНИЕ -- TECHNOLOGY TRANSFER
Аннотация: Significant progress in the promotion of procedural technologies associated with the transplantation of hematopoietic stem cells caused a rapid increase in activity. The exchange of hematopoietic stem cells for unrelated donor transplantations is now much easier due to the relevant international professional structures and organizations established to support cooperation and standard setting, as well as rules for the functioning of both national donor registries and cord blood banks. These processes are increasing every year and are contributing to the outpacing rates of development in this area. Products within their country should be regulated by the competent government authorities. This study analyzes the work of international and national levels of support for transplantation activity in the field of unrelated hematopoietic stem cell transplantation, the standardization order of technologies, as well as data that justify the need to create a network of donated umbilical cord blood banks in Ukraine as a factor in the development of allogeneic transplantation. This will promote the accessibility of international standards for the treatment of serious diseases for Ukrainian citizens
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Endometrial stromal cells: isolation, expansion, morphological and functional properties [Текст] / A. V. Zlatska [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 197-202. - Bibliogr. at the end of the art.

MeSH-главная:
СТРОМАЛЬНЫЕ КЛЕТКИ -- STROMAL CELLS (ультраструктура, физиология)
IN VITRO МЕТОДЫ -- IN VITRO TECHNIQUES (использование, тенденции)
ЭНДОМЕТРИЙ -- ENDOMETRIUM (анатомия и гистология)
КОЛОНИЕОБРАЗУЮЩИХ ЕДИНИЦ АНАЛИЗ -- COLONY-FORMING UNITS ASSAY (методы, тенденции)
МЕДИЦИНА РЕГЕНЕРАТИВНАЯ -- REGENERATIVE MEDICINE (методы, организация и управление, тенденции)
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS
Аннотация: We aimed to study biological properties of human endometrial stromal cells in vitro. Materials and methods: The endometrium samples (n = 5) were obtained by biopsy at the first phase of the menstrual cycle from women with endometrial hypoplasia. In all cases, a voluntary written informed consent was obtained from the patients. Endometrial fragments were dissociated by enzymatic treatment. The cells were cultured in DMEM/F12 supplemented with 10% FBS, 2 mМ L-glutamine and 1 ng/ml FGF-2 in a multi-gas incubator at 5% CO2 and 5% O2. At P3 the cells were subjected to immunophenotyping, multilineage differentiation, karyotype stability and colony forming efficiency. The cell secretome was assessed by BioRad Multiplex immunoassay kit. Results: Primary population of endometrial cells was heterogeneous and contained cells with fibroblast-like and epithelial-like morphology, but at P3 the majority of cell population had fibroblast-like morphology. The cells possessed typical for MSCs phenotype CD90+CD105+CD73+CD34-CD45-HLA-DR-. The cells also expressed CD140a, CD140b, CD146, and CD166 antigents; and were negative for CD106, CD184, CD271, and CD325. Cell doubling time was 29.6 ± 1.3 h. The cells were capable of directed osteogenic, adipogenic and chondrogenic differentiation. The cells showed 35.7% colony forming efficiency and a tendency to 3D spheroid formation. The GTG-banding assay confirmed the stability of eMSC karyotype during long-term culturing (up to P8). After 48 h incubation period in serum-free medium eMSC secreted anti-inflammatory IL-1ra, as well as IL-6, IL-8 and IFNγ, angiogenic factors VEGF, GM-CSF and FGF-2, chemokines IP-10 and MCP-1. Conclusion: Thus, cultured endometrial stromal cells meet minimal ISCT criteria for MSC. Proliferative potential, karyotype stability, multilineage plasticity and secretome profile make eMSC an attractive object for the regenerative medicine use
Доп.точки доступа:
Zlatska, A. V.
Rodnichenko, A. E.
Gubar, O. S.
Zubov, D. O.
Novikova, S. N.
Vasyliev, R. G.

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Content of stem tumor CD133⁺ cells in brain neoplasms of different histological type [Текст] / N. I. Lisyaniy [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 219-223. - Bibliogr. at the end of the art.

MeSH-главная:
НОВООБРАЗОВАНИЙ МАРКЕРЫ БИОЛОГИЧЕСКИЕ -- TUMOR MARKERS, BIOLOGICAL (анализ)
МОЗГА ГОЛОВНОГО НОВООБРАЗОВАНИЯ -- BRAIN NEOPLASMS (диагностика, ультраструктура, этиология)
АНАПЛАЗИЯ -- ANAPLASIA (этиология)
ИММУНОГИСТОХИМИЯ -- IMMUNOHISTOCHEMISTRY (использование, тенденции)
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS
Кл.слова (ненормированные):
CD133⁺
Аннотация: Today, there are conflicting data on the content of cancer stem cells responsible for recurrence and resistance to chemotherapy in tumors of human brain. The aim of the study was to analyze the content of CD133⁺cells in different brain tumors by immunofluorescence assay and immunohistochemical method. Materials and Methods: The samples of different brain tumors removed during neurosurgical operations were studied for CD133 expression. Results: Immunofluorescence assay of tumor imprints revealed CD133⁺cells in 40–85% of tumors regardless of histological type. In malignant tumors, the count of CD133⁺cells was higher than in benign tumors. Immunohistochemical method used for detection of CD133⁺cells was less sensitive than immunofluorescence technique. The number of CD133⁺cells may vary even in tumors of the same histological type. In 20–30% of malignant tumors (glioblastomas, medulloblastomas), the content of CD133⁺cells was very low or not detected at all. Conclusions: In tumors of the brain of different genesis and degree of anaplasia CD133⁺cells are found out. In malignant tumors (glioblastomas and medulloblastomas), CD133⁺cells are much more frequently detected than in benign brain tumors. The content of CD133⁺cells in brain tumors is highly variable being small and some malignant tumors, indicating low predictive and diagnostic value of cancer stem cell content in clinical practice
Доп.точки доступа:
Lisyaniy, N. I.
Stanetskaya, D. N.
Lisyaniy, A. N.
Belskaya, L. N.

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Comparison of various tissue and cell therapy approaches when restoring ovarian, hepatic and kidney’s function after chemotherapy-induced ovarian failure [Текст] / M. M. Kozub [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 181-185. - Bibliogr. at the end of the art.

MeSH-главная:
БОЛЕЗНЬ, МОДЕЛИ НА ЖИВОТНЫХ -- DISEASE MODELS, ANIMAL
ПЕРВИЧНАЯ ЯИЧНИКОВАЯ НЕДОСТАТОЧНОСТЬ -- PRIMARY OVARIAN INSUFFICIENCY (патофизиология, химически вызванный, этиология)
КЛЕТОЧНАЯ И ТКАНЕВАЯ ТЕРАПИЯ -- CELL- AND TISSUE-BASED THERAPY (использование, статистика, тенденции)
СТВОЛОВЫХ КЛЕТОК МЕЗЕНХИМНЫХ ТРАНСПЛАНТАЦИЯ -- MESENCHYMAL STEM CELL TRANSPLANTATION (использование, методы, статистика, тенденции)
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS
Аннотация: About 1% of women suffer from premature ovarian failure, which leads to a significant deterioration in the life quality. Most often this condition is caused by performed chemotherapy, autoimmune diseases, surgery performed on ovaries, uterus, or fallopian tubes. The aim of this study was to compare different approaches of tissue and cell therapy in restoring the sexual function in case of ovarian failure induced by chemotherapy. Materials and Methods: The study was carried out in BALB/c mice with the modeled ovarian failure, induced by cyclophosphamide and busulfan. The restoration dynamics of ovarian and sexual function, liver and kidneys after application of cryopreserved explants, cryoextract, placental mesenchymal stem cells, those of adipose tissue has been studied. Results: It has been shown that the use of various methods of cell and tissue therapy has comparable efficacy when treating an ovarian failure induced by chemotherapy. The most rapid and complete restoration of the reproductive system, liver and kidneys was observed when using the placental explants, extract and cells, but not with the use of mesenchymal stem cells of adipose tissue. No recovery of fertility in this experiment was observed. Conclusion: Various methods of cellular and tissue therapy are perspective in treatment of the chemotherapy complications. More effective are placental derivates
Доп.точки доступа:
Kozub, M. M.
Prokopiuk, V. Y.
Skibina, K. R.
Prokopiuk, О. V.
Kozub, N. I.

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Chekhun, V. F.
On the 40th anniversary of the international symposium “The role of stem cells in leukemo-and carcinogenesis»: in Kyiv again [Текст] / V. F. Chekhun // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 162-163

MeSH-главная:
ЮБИЛЕИ И ДРУГИЕ ВАЖНЫЕ СОБЫТИЯ -- ANNIVERSARIES AND SPECIAL EVENTS
СЪЕЗДЫ, СИМПОЗИУМЫ -- CONVENTIONS, SYMPOSIUMS
СТВОЛОВЫЕ КЛЕТКИ -- STEM CELLS
НЕОПЛАСТИЧЕСКИЕ ПРОЦЕССЫ -- NEOPLASTIC PROCESSES
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10.

Bezdieniezhnykh, N. O.
Scientific-practical and legal problems of implementation of the personalized medicine [Текст] / N. O. Bezdieniezhnykh, V. V. Reznikova, O. V. Rossylna // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 229-233

MeSH-главная:
ЗДОРОВЬЯ ИНДИВИДУАЛЬНОГО ОХРАНА -- PERSONAL HEALTH SERVICES (законодательство и юриспруденция, методы, организация и управление, тенденции)
МЕДИЦИНСКОЙ ПОМОЩИ ПЛАНИРОВАНИЕ ИНДИВИДУАЛЬНОЕ -- ADVANCE CARE PLANNING (законодательство и юриспруденция, использование, организация и управление, статистика, тенденции)
МЕЖДУНАРОДНОЕ СОТРУДНИЧЕСТВО -- INTERNATIONAL COOPERATION (законодательство и юриспруденция)
ЛЕЧЕБНО-ДИАГНОСТИЧЕСКАЯ ТАКТИКА -- CRITICAL PATHWAYS (законодательство и юриспруденция, организация и управление, тенденции)
Аннотация: The article is devoted to the comprehensive analysis of scientific, practical and legal issues of personalized medicine that is a rapidly developing science-driven approach to healthcare. It is concluded that there is lack of general legal framework for the encouragement of scientific researches and practical implementation in this field. The article shows foreign experience and prospects for the introduction of personalized medicine as a key concept of healthcare system, which is based on a selection of diagnostic, therapeutic and preventive measures that would be the most effective for a particular person in view of individual characteristics. The conclusions and proposals to improve the current legislation and development of personalized medicine in Ukraine are suggested
Доп.точки доступа:
Reznikova, V. V.
Rossylna, O. V.

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11.

Association of CD44⁺CD24^-/low with markers of aggressiveness and plasticity of cell lines and tumors of patients with breast cancer [Текст] / V. F. Chekhun [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 203-211. - Bibliogr. at the end of the art.

MeSH-главная:
МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS (осложнения, патофизиология, этиология)
НОВООБРАЗОВАНИЙ МАРКЕРЫ БИОЛОГИЧЕСКИЕ -- TUMOR MARKERS, BIOLOGICAL (анализ)
ЦИТОСТАТИЧЕСКИЕ СРЕДСТВА -- CYTOSTATIC AGENTS (анализ, терапевтическое применение)
ЛЕКАРСТВЕННАЯ УСТОЙЧИВОСТЬ НОВООБРАЗОВАНИЙ -- DRUG RESISTANCE, NEOPLASM (физиология)
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
Аннотация: To search for additional molecular-biological markers of cancer stem cell (CSC) involved in the development of intra-tumor heterogeneity for the detection of features of the breast cancer (BC) pathogenesis. Materials and methods: Expression of estrogen receptors (ER), progesterone receptors (PR), Her2/neu, E- and N-cadherin, CD24, CD44, Bcl-2, Bax, Slug, P-gp, glutathione-S-transferase (GST) and metallothionein in cell lines was determined by the immunocytochemical method. Expression of ER, PR, Her2/neu, CD24 and CD44 in the surgical material of BC patients were determined by the immunohistochemical method. The levels of the miRNA were determined using real-time polymerase chain reaction. Results: Cells of high-grade malignancy (HGM), MDA-MB-231 and MDA-MB-468 are characterized by high expression of stem cell markers compared to the cells of low-grade malignancy (LGM), T47D and MCF-7: CD44 levels in T47D and MCF-7 cells were in range of 72-79 points, which is significantly lower than in HGM cells (p 0.05). Also, HGM cells with the properties of CSC were characterized by high expression of antiapoptotic proteins, the transcription factor Slug, and low levels of proapoptotic protein Bax (p 0.05) compared to LGM cells. In cells with CSC characteristics an increased expression of transferrin and its receptor, ferritin, fentorin and hepcidin was revealed indicating activation of the endogenous iron metabolism. The characteristic feature of HGM cells with CSC phenotype were the increased levels of oncogenic miR-221, -155 and -10b by 60%, 92% and 78%, respectively, and decreased levels of oncosuppressive miR-29b, -34a and -200b by 8.4 ± 0.3, 4.6 ± 0.2, and 3.4 ± 0.6 times compared to MCF-7 line cells. It has been established that the development of resistance to cytostatics is accompanied by increased aggressiveness of tumor cells, loss of expression of hormonal receptors and acquiring of stem phenotype. In particular, increased expression of P-gp was observed in BC cells during the development of resistance to doxorubicin, of GST during the development of resistance to cisplatin along with increased CD44 expression (p 0.05). We have revealed the relation between the presence of cells with the CSC phenotype (CD44+CD24-/low) and clinical and pathological characteristics of BC patients, their survival and BC sensitivity to neoadjuvant therapy (p > 0.05). Conclusions: The dependence between the expression of CSC markers and the degree of malignancy of tumor cells, development of resistance to cytostatics in vitro was established as well as the predictive value of the detection of the CSC for the individual prognosis of the BC course and sensitivity of the tumors to the treatment
Доп.точки доступа:
Chekhun, V. F.
Lukianova, N. Y.
Chekhun, S. V.
Bezdieniezhnykh, N. O.
Zadvomiy, T. V.
Borikun, T. V.
Polishchuk, L. Z.
Klyusov, O. M.

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12.

Are CD44⁺CD24^- cells the assumed cancer stem cells in breast cancer? [Текст] / D. E. Ryspayeva [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 224-228. - Bibliogr. at the end of the art.

MeSH-главная:
МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS (патофизиология, этиология)
СТВОЛОВЫХ КЛЕТОК ИССЛЕДОВАНИЕ -- STEM CELL RESEARCH
ГИСТОЦИТОХИМИЯ -- HISTOCYTOCHEMISTRY (использование, тенденции)
АНТИГЕНЫ CD24 -- ANTIGENS, CD24 (анализ)
АНТИГЕНЫ CD44 -- ANTIGENS, CD44 (анализ)
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS
Кл.слова (ненормированные):
раковые стволовые клетки
Аннотация: Identification and characterization of the population of cancer stem cells (CSC) depends on several cellular markers, which combination is specific for the phenotype of CSC in the corresponding tumor. Several markers of CSC have already been identified in breast cancer (BC), but there are no universal indicators that could specifically identify the CSC in BC. Aims: To determine the validation of the CSC model for cell surface markers such as CD44 and CD24 and their clinical significance. Materials and methods: Primary tumor samples of 45 patients with invasive BC without chemotherapy prior to surgery exposure were examined in paraffin blocks. CD44 and CD24 antigens expression was evaluated by the percentage of positive cells using different chromogens and the MultiVision detection system by immunohistochemical method. In this research the evaluation was determined by the following criteria: (-), negative - expression in < 10% of tumor cells; (+), positive - expression in ≥10% of cells. The same scoring system was applied for the expression of CD44+/CD24-. Results: 62.2% of investigated patients are patients older than 50 years and most of them with stage II of disease (71.0%) and luminal tumor subtypes (68.9%). We analysed the expression of CD44, CD24 and CD44+/CD24- for diffe-rent patients with dividing them into two groups. The group A consists of patients with unfavorable prognosis (relapses and metastases have occurred in the first three years after diagnosis), and the group B - with a favourable prognosis (the development of metastases after three years). Median disease-free survival in the group A is 19 months, in the group B - 46 months. The difference between the overall survival (OS) curves in the groups A and B is statistically significant (p < 0.001), the risk of death was higher in the group A (hazard ratio (HR) 5.9; confidence interval (CI) 2.3-15.2). The content of CD44 cells did not differ statistically between groups A and B (p = 0.18), but there was a tendency for increasing in OS with the existence of CD44+ cells (p = 0.056). The distribution of the expression of CD24 marker did not differ between the groups (p = 0.36) as well as the OS curves (p = 0.59). Analysis of the expression of CD44+/CD24- which were considered as possible CSC, revealed a paradoxical increase (p = 0.03) of the frequency in patients of the group B (40.9%) compared to the group A (8.7%). Nevertheless, the comparison of the clinical outcomes did not reveal a statistically significant difference in the survival curves in the groups with existence and absence of CD44+/CD24- expression (p = 0.08). The analysis showed the increasing of the risk of worse clinical outcomes in the cases of expression absence of CD44+/CD24- (HR 2.8; CI 1.1-6.8). Conclusions: As a result of our research, the analysis of the quantity of assumed stem cells of the BC, which were identified by immunohistochemistry as CD44 and CD24 cells, failed to detect a statistically significant relation between groups of patients with different prognosis, and the identification of their expression is not enough for the characteristics of CSC. The obtained data demonstrating the worst clinical outcome in the cases of absence of CD44+/CD24- expression apparently require further investigations and the validation of the immunohistochemical method with the determination of the cut-off line in defining of CD44 and CD24 status
Доп.точки доступа:
Ryspayeva, D. E.
Smolanka, I. I.
Dudnichenko, A. S.
Lyashenko, A. A.
Grinevich, Yu. A.
Gurianov, V. G.
Koshubarova, M. V.
Seleznev, A. A.

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13.

Anti-cancer efficiency of natural killer cells differentiated from human adipose tissue-derived mesenchymal stem cells and transfected with miRNA150 [Текст] / A. Karlitepe [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 212-218. - Bibliogr. at the end of the art.

MeSH-главная:
СТРОМАЛЬНЫЕ КЛЕТКИ МЕЗЕНХИМНЫЕ -- MESENCHYMAL STROMAL CELLS (секреция, ультраструктура)
ИММУНОГИСТОХИМИЯ -- IMMUNOHISTOCHEMISTRY (использование, тенденции)
КЛЕТКИ-КИЛЛЕРЫ ЕСТЕСТВЕННЫЕ -- KILLER CELLS, NATURAL (ультраструктура)
ПОДЖЕЛУДОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- PANCREATIC NEOPLASMS (патофизиология, этиология)
ГЕНЫ СУПРЕССОРЫ ОПУХОЛЕВОГО РОСТА -- GENES, TUMOR SUPPRESSOR (физиология)
ИММУНОТЕРАПИЯ -- IMMUNOTHERAPY (тенденции)
СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL
ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS
Аннотация: The aim of this study is to investigate the effects of miR150 transfection on NK-like cells differentiated from adipose tissue derived mesenchymal stem cells (AD-MSCs). Methods: NK-like cells were differentiated from AD-MSCs and activated by miR150 transfection. Transfected/non-transfected NK-like cells were characterized by immunohistochemical and RTPCR analyzes. Apoptotic efficiency of the transfected/non-transfected NK-like cells on pancreatic cancer cells PANC1 were determined by TUNEL and RT-PCR. Results: In miR150-transfected cells, the increased expression of NK cell-specific genes such as GZMB, KIR2DL2, CD16, CD56, NKG2D, NKp46 and increased immunoreactivity of NK cell-specific surface marker CD314 (NKG2D) were evident. TUNEL assays showed that NK-like cells with/without transfection induced apoptosis in PANC1 cells in the same manner. The decrease in oncogene expression and the increase in the tumor suppressor gene expression in PANC1 cells upon co-culture with NK-like cells differentiated from AD-MSCs were more prominent following miRNA150 transfection. Conclusion: It was shown in vitro that NK-like cells could be obtained by differentiation from AD-MSCs and their efficiency could be increased via miR150 transfection. The results are encouraging for further clinical studies in improvement of immunotherapeutic approaches for cancer therapy
Доп.точки доступа:
Karlitepe, A.
Kabadayi, H.
Vatansever, S.
Gurdal, M.
Gunduz, C.
Ercan, G.

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