Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Osadchuk T. V., Shybyryn O. V., Kibirev V. K.
Заглавие : Chemical structure and properties of low-molecular furin inhibitors
Параллельн. заглавия :Хімічна будова та властивості низькомолекулярних інгібіторів фурину
Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 6. - С. 5-25 (Шифр УУ1/2016/88/6)
Примечания : Bibliogr. at the end of the art.
MeSH-главная: ПЕПТИДОМИМЕТИКИ -- PEPTIDOMIMETICS
ФУРИН -- FURIN
ОБЗОР -- REVIEW
ОНКОЛОГИЯ МЕДИЦИНСКАЯ -- MEDICAL ONCOLOGY
БИОДОСТУПНОСТЬ -- BIOLOGICAL AVAILABILITY
Аннотация: The review is devoted to the analysis of the relationship between a chemical structure and properties of low-molecular weight inhibitors of furin, the most studied proprotein convertase, which is involved in the development of some pathologies, such as oncologic diseases, viral and bacterial infections, etc. The latest data concerning the influence of peptides, pseudo-peptides, aromatic and heterocyclic compounds, some natural ones such as flavonoids, coumarins, and others on enzyme inactivation are considered. The power of furin inhibition is shown to rise with the increasing number of positively charged groups in the structure of these compounds. Peptidomimetics (Ki = 5-8 pM) are shown to be the most effective furin inhibitors. The synthesized substances, however, have not been used in practical application yet. Nowadays it is very important to find more selective inhibitors, improve their stability, bioavailability and safety for the human organism
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Моlodchenkova О. О., Adamovskaya V. G., Ciselskaya L. Y., Bezkrovnaya L. Ya., Kаrtuzova T. V., Iablonska V. B.
Заглавие : Purification and properties of lipoxygenase from wheat seedlings infected by Fusarium graminearum and treated by salicylic acid
Параллельн. заглавия :Очистка та властивості ліпоксигенази із проростків пшениці, інфікованих Fusarium graminearum та оброблених саліциловою кислотою
Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 6. - С. 26-34 (Шифр УУ1/2016/88/6)
Примечания : Bibliogr. at the end of the art.
MeSH-главная: ЛИПОКСИГЕНАЗА -- LIPOXYGENASE
ФУЗАРИУМ -- FUSARIUM
САЛИЦИЛОВАЯ КИСЛОТА -- SALICYLIC ACID
АГГЛЮТИНИНЫ ПРОРОСТКОВ ПШЕНИЦЫ -- WHEAT GERM AGGLUTININS
Аннотация: Lipoxygenase from wheat seedlings in normal conditions, infected by Fusarium graminearum and treated by salicylic acid was isolated. The isolated enzyme was purified by the methods of salting-out (60% ammonium sulphate), dialysis, gel-filtration and ion-exchange chromatography. Specific activity of the purified enzyme was 8.0-12.5 ΔЕ234/mg of protein, degree of purification – 11.6-15.3 times. The enzyme yield was 18.3-27.9%. Molecular mass of lipoxygenase is 90 kDa, amino acid composition is distinguished by a high content of glutamic acid, proline, valine, isoleucine, leucine and low level of histidine, tyrosine, phenylalanine, threonine, tryptophan, cystein. Research of lipoxygenase substrate dependence indicated that the enzyme catalysed with the maximum velocity of the reaction of arachidonic acid oxidation at a substrate concentration of 4.5 mM at pH 7.2, the reaction of linoleic acid oxidation at a substrate concentration of 4.5 mM at pH 7.2 and the reaction of linolenic acid oxidation at a substrate concentration of 9.0 mM at pH 8.0. The change of wheat lipoxygenase activity depending on genotype resistance to Fusarium graminearum and millieu of germination was shown. One of the manifestations of the protective effect of salicylic acid is its ability to induce changes of lipoxygenase activity
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Holota Yu. V., Olefir Ya. A., Dovbynchuk T. V., Tolstanova G. M.
Заглавие : Carbohydrate composition of rat intestine surface mucus layer after ceftriaxone treatment
Параллельн. заглавия :Вуглеводний склад поверхневого слизу кишечника щурів після введення цефтриаксону
Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 6. - С. 35-44 (Шифр УУ1/2016/88/6)
Примечания : Bibliogr. at the end of the art.
MeSH-главная: МОДЕЛИ НА ЖИВОТНЫХ -- MODELS, ANIMAL
КИШЕЧНИКА СЛИЗИСТАЯ ОБОЛОЧКА -- INTESTINAL MUCOSA
ЦЕФТРИАКСОН -- CEFTRIAXONE
ГЛИКОПРОТЕИНЫ -- GLYCOPROTEINS
КРЫСЫ ИНБРЕДНОЙ ЛИНИИ BB -- RATS, INBRED BB
УГЛЕВОДНЫЙ ОБМЕН -- CARBOHYDRATE METABOLISM
Аннотация: The epidemiological studies have shown that antibiotic treatment increases the susceptibility to inflammatory bowel disease development. The disturbance of mucus layer integrity might be one of the possible mechanisms. The aim of the present study was to investigate the effect of antibiotic ceftriaxone treatment on glycoproteins level and its carbohydrate composition in surface mucus layer of rat intestine. The study was done on male Wistar rats (140-160 g). Ceftriaxone (300 mg/kg, i.m.) was administered once a day for 14 days. The surface mucus from terminal ileum and colon were collected on the 15th, 29th and 72nd days of the experiment. Total level of mucus glycoproteins, hexoses, hexosamines, fucose and sialic acids were measured. Ceftriaxone administration did not affect the levels of glycoproteins in rat ileum. In the colon, the levels of glycoprotein were 1.3-fold decreased (Р 0.05) on the 72nd day of the experiment. These changes were accompanied by the 1.2-fold decrease of hexoses (Р 0.05) and 3.1-fold (Р 0.05) decrease of fucose level and 1.5-fold (Р 0.05) increase of the levels of sialic acids in the surface mucus of the rat colon. Thus, ceftriaxone administration induces the long-term changes in the levels of glycoproteins and carbohydrates composition in the rat colon surface mucus. This could potentially explain the susceptibility to inflammatory bowel diseases development
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Afanasieva K. S., Chopei M. I., Lozovik A. V., Rushkovsky S. R., Sivolob A. V.
Заглавие : Redistribution of DNA loop domains in human lymphocytes under blast transformation with interleukin 2
Параллельн. заглавия :Перерозподіл петельних доменів ДНК у лімфоцитах людини у разі бласттрансформації за впливу інтерлейкіну 2
Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 6. - С. 45-51 (Шифр УУ1/2016/88/6)
Примечания : Bibliogr. at the end of the art.
MeSH-главная: ТРАНСКРИПЦИЮ АКТИВИРУЮЩИЕ ФАКТОРЫ -- ACTIVATING TRANSCRIPTION FACTORS
ЛИМФОЦИТЫ -- LYMPHOCYTES
ИНТЕРЛЕЙКИН-2 -- INTERLEUKIN-2
ДНК-КОМЕТ МЕТОД -- COMET ASSAY
КЛЕТКИ ЯДРО -- CELL NUCLEUS
Аннотация: At higher order levels chromatin fibers in interphase nuclei are organized into loop domains. Gene regulatory elements (promoters and enhancers) are often located near the sites of loop attachments. Therefore, loop domains play a key role in regulation of cell transcriptional activity. We investigated the kinetics of DNA loop exit during single cell gel electrophoresis (the comet assay) of nucleoids obtained from two cell types that differ in their synthetic activity – human lymphocytes and lymphoblasts. Lymphocyte activation and transformation into lymphoblasts (blast transformation) was performed with interleukin 2. The results obtained suggest that a rearrangement of the loops occurs after lymphocyte activation. After blast transformation we observed an increase of the amount of loop domains on the surface of nucleoids against a decrease of the inner loop fraction. Therefore, the comet assay can be used for detection of large-scale changes in the cell nucleus that follow changes in cell functional state
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Minchenko O. H., Tsymbal D. O., Minchenko D. O., Kubaychuk O. O.
Заглавие : Hypoxic regulation of MYBL1, MEST, TCF3, TCF8, GTF2B, GTF2F2 and SNAI2 genes expression in U87 glioma cells upon IRE1 inhibition
Параллельн. заглавия :Гіпоксична регуляція експресії генів MYBL1, MEST, TCF3, TCF8, GTF2B, GTF2F2 та SNAI2 у клітинах гліоми U87 за інгібування IRE1
Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 6. - С. 52-62 (Шифр УУ1/2016/88/6)
Примечания : Bibliogr. at the end of the art.
MeSH-главная: ГЕННАЯ ЭКСПРЕССИЯ -- GENE EXPRESSION
ГЛИОМА -- GLIOMA
ПРОТООНКОГЕНЫ -- PROTO-ONCOGENES
ГИПОКСИЯ-ИНДУЦИРУЕМЫЙ ФАКТОР 1 -- HYPOXIA-INDUCIBLE FACTOR 1
КЛЕТОК КОНТАКТНОЕ ИНГИБИРОВАНИЕ -- CONTACT INHIBITION
Аннотация: We investigated the impact of IRE1/ERN1 (inositol requiring enzyme 1/endoplasmic reticulum to nucleus signaling 1) knockdown on hypoxic regulation of the expression of a subset of proliferation and migration-related genes in U87 glioma cells. It was shown that hypoxia leads to up-regulation of the expression of MEST and SNAI2, to down-regulation – of MYBL1, TCF8 and GTF2F2 genes at the mRNA level in control glioma cells. At the same time hypoxia did not affect the expression of TCF3 and GTF2B transcription factor genes. In turn, inhibition of IRE1 modified the effect of hypoxia on the expression of all studied genes, except MYBL1 and GTF2B. For instance, IRE1 knockdown decreased sensitivity to hypoxia of the expression of MEST, TCF8 and SNAI2 genes and increased sensitivity to hypoxia of GTF2F2 expression. At the same time, IRE1 inhibition introduced sensitivity to hypoxia of the expression of TCF3 gene in glioma cells. The present study demonstrated that the inhibition of IRE1 in glioma cells affected the hypoxic regulation of the expression of studied genes in various directions, though hypoxic conditions did not abolish the effect of IRE1 inhibition on the expression of respective genes. To the contrary, in case of SNAI2, GTF2F2 and MEST hypoxic conditions magnified the effect of IRE1 inhibition on the expression of respective genes in glioma cells
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Myronovskij S. L., Boiko N. M., Chumak V. V., Shorobura M. S., Lootsyk M. D., Stoika R. S., Kit Yu. Ya.
Заглавие : The characteristics of antibodies of mice immunized by human unconventional myosin 1c
Параллельн. заглавия :Характеристика антитіл мишей, імунізованих неконвенційним міозином людини
Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 6. - С. 63-69 (Шифр УУ1/2016/88/6)
Примечания : Bibliogr. at the end of the art.
MeSH-главная: МОДЕЛИ НА ЖИВОТНЫХ -- MODELS, ANIMAL
МИОЗИНЫ -- MYOSINS
АНТИГЕН-АНТИТЕЛО РЕАКЦИИ -- ANTIGEN-ANTIBODY REACTIONS
АСЦИТИЧЕСКАЯ ЖИДКОСТЬ -- ASCITIC FLUID
АУТОАНТИТЕЛА -- AUTOANTIBODIES
Аннотация: Specific antibodies produced against a protein of interest are invaluable tools for monitoring the protein structure, intracellular location and biological activity. Inoculation of murine lymphoma cells into the peritoneal cavity of immunized mice provides generation of ascitic fluid containing a significant amount of antibody with desired antigen specificity. Here we demonstrated that the intraperitoneal administration of murine lymphoma NK/Ly cells in mice immunized with 48 kDa isoform of human blood serum unconventional myosin 1c leads to generation of ascitic fluid that contained specific IgG-antibodies. These antibodies were capable of binding of the unconventional myosin 1c isolated from blood serum of patients with multiple sclerosis, rheumatoid arthritis and systemic lupus erythematosis, and could be used for diagnostics of several autoimmune diseases, the multiple sclerosis in particular
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Akimov O. Ye., Kostenko V. O.
Заглавие : Functioning of nitric oxide cycle in gastric mucosa of rats under excessive combined intake of sodium nitrate and fluoride
Параллельн. заглавия :Функціонування циклу оксиду азоту в слизовій оболонці шлунка щурів за надмірного комбінованого надходження нітрату та фториду натрію
Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 6. - С. 70-75 (Шифр УУ1/2016/88/6)
Примечания : Bibliogr. at the end of the art.
MeSH-главная: МОДЕЛИ НА ЖИВОТНЫХ -- MODELS, ANIMAL
ЖЕЛУДКА СЛИЗИСТАЯ -- GASTRIC MUCOSA
АЗОТА ОКСИДЫ -- NITROGEN OXIDES
НИТРАТРЕДУКТАЗЫ -- NITRATE REDUCTASES
АРГИНАЗА -- ARGINASE
НАТРИЯ ФТОРИД -- SODIUM FLUORIDE
Аннотация: In the article the function of nitric oxide (·NO) cycle in rat’s gastric mucosa was assessed under excessive combined chronic fluoride and nitrate intake during 30 days. It was estimated that general nitric oxide synthase activity (NOS) was increased during excessive sodium fluoride intake meanwhile influence on general nitrate reduction was not statistically significant, but general nitrite reduction was increased. General arginase activity decreased. Excessive sodium nitrate intake decreased NOS activity, but increased nitrate, nitrite reduction and general arginase activity. Combined sodium nitrate and fluoride intake increased NOS by 18.9%, nitrate reduction by 71.7%, nitrite by 161.5%, arginase activity increase by 61.4%. The highest amounts of peroxynitrite were obtained from excessive sodium fluoride intake group, excessive sodium nitrate intake showed the lowest levels and combined excessive sodium nitrate and fluoride intake levels had intermediate results. Summarizing the data obtained, the authors have made a conclusion that combined excessive sodium nitrate and fluoride intake creates optimal conditions for functioning of nitrate-nitrite reductases
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Hamit Yasar Ellidag, Esin Eren, Mehmet Akdag, Ozlem, Kemal, Necat
Заглавие : The relationship between serum ferritin levels and serum lipids and HDL function with respect to age and gender
Параллельн. заглавия :Взаємозв’язок між рівнем сироваткових феритину, ліпідів і ліпопротеїнів високої щільності з урахуванням віку і статі людей
Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 6. - С. 76-86 (Шифр УУ1/2016/88/6)
Примечания : Bibliogr. at the end of the art.
MeSH-главная: ФЕРРИТИНЫ -- FERRITINS
ЛИПИДНЫЙ ОБМЕН -- LIPID METABOLISM
ВОЗРАСТНЫЕ ФАКТОРЫ -- AGE FACTORS
ОБМЕНА ВЕЩЕСТВ БОЛЕЗНИ -- METABOLIC DISEASES
ЛИПОПРОТЕИНЫ ВЫСОКОЙ ПЛОТНОСТИ -- LIPOPROTEINS, HDL
ЛИПОПРОТЕИНЫ НИЗКОЙ ПЛОТНОСТИ -- LIPOPROTEINS, LDL
Аннотация: Elevated serum ferritin (SFer) levels have been associated with chronic diseases such as coronary heart disease and diabetes mellitus type 2. The aim of this study was to examine the relationship between SFer levels and serum lipid parameters, and how this relation changes in terms of age and gender. Additionally, we investigated a possible relationship between SFer levels and high-density lipoprotein (HDL) function. SFer levels and lipid panel (total cholesterol (TC), triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C) and HDL-C) of 4205 people (3139 women, 1066 men) were examined retrospectively. Study population was classified according to age and gender. Separately, 100 subjects (52 women, 48 men) were randomly recruited to investigate the relation between SFer levels, and HDL dependent paraoxonase-1 (PON1) and arylesterase (ARE) activities. In all age groups, women’s SFer levels were found to be significantly lower and HDL-C levels significantly higher compared to men. In the 50-70 ages range, TC and LDL-C levels of women were found to be significantly higher than those of men (P 0.01). SFer levels tended to increase with age in women. Correlation analyses revealed a negative correlation between levels of SFer and HDL-C, while positive correlations existed between levels of SFer, and TC, TG and LDL-C. There was no significant correlation between SFer levels and PON1 or ARE activities. The finding that increased SFer levels are accompanied by increased serum TC, TG and LDL-C levels may help us to explain the increased risk of metabolic disorders and cardiovascular disease in postmenopausal women
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Malysheva Kh. V., Spasyuk I. M., Pavlenko O. K., Stoika R. S., Korchynskyi O. G.
Заглавие : Generation of optimized preparations of bone morphogenetic proteins for bone regeneration
Параллельн. заглавия :Створення оптимізованих препаратів морфогенних протеїнів кістки для регенерації кісткової тканини
Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 6. - С. 87-97 (Шифр УУ1/2016/88/6)
Примечания : Bibliogr. at the end of the art.
MeSH-главная: КОСТНАЯ РЕГЕНЕРАЦИЯ -- BONE REGENERATION
КОСТИ МОРФОГЕНЕТИЧЕСКИЕ БЕЛКИ -- BONE MORPHOGENETIC PROTEINS
ОСТЕОГЕНЕЗ -- OSTEOGENESIS
ФАРМАЦЕВТИЧЕСКИЕ ПРЕПАРАТЫ -- PHARMACEUTICAL PREPARATIONS
ИННОВАЦИЙ РАСПРОСТРАНЕНИЕ -- DIFFUSION OF INNOVATION
Аннотация: Correction of inherited skeletal abnormalities, traumas affecting wide bone areas and non-healing fractures require efficient bone formation and regeneration. Bone morphogenetic proteins (BMPs) are signaling molecules that play a crucial role in bone and cartilage formation and regeneration. Osteoinductive properties of existing hydroxyapatite-based osteoplastic materials are frequently insufficient for efficient bone regeneration, thus raising a requirement for novel matrices involving BMPs for highly efficient local induction of bone formation at the area of the bone defect. The aim of this study was conducting in vitro optimization of osteoinductive properties of recombinant BMPs preparations to be used in bone regenerative practice. Recombinant BMPs were produced in human embryonic kidney 293 cells upon their transfection or co-transfection with plasmids expressing BMP2 and BMP7 at different ratios. A quality of BMP preps was validated based on their ability to induce in vitro osteoblast differentiation of C2C12 cells. Alkaline phosphatase that is widely used as a marker of osteoblast differentiation was measured spectrophotometrically. We found that the most effective inducer of osteoblast differentiation was recombinant BMP preparation produced upon cotransfection of 85% of BMP2 and 15% of BMP7 plasmids, that is most likely due to generation of conditions most favorable for formation of BMP2/7 heterodimers. Frozen BMP2/7 preparations stored for 3 h in experimental setup and for several weeks in routine work do not lose their osteoinductive properties compared with freshly prepared BMP2/7 preparations and can be successfully used for generation of highly efficient bone regenerative matrices
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ezepchuk Yu. V., Kolybo D. V.
Заглавие : Nobel laureate Ilya I. Metchnikoff (1845-1916). Life story and scientific heritage
Параллельн. заглавия :Нобелівський лауреат Ілля Ілліч Мечников (1845–1916). Історія життя та наукова спадщина
Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 6. - С. 98-109 (Шифр УУ1/2016/88/6)
Примечания : Bibliogr. at the end of the art.
MeSH-главная: НОБЕЛЕВСКАЯ ПРЕМИЯ -- NOBEL PRIZE
ЛИЧНОСТИ ВЫДАЮЩИЕСЯ -- FAMOUS PERSONS
БИОГРАФИЯ КАК ТЕМА -- BIOGRAPHY AS TOPIC
Аннотация: A century of science after I. Metchnikoff’s death demonstrated the truth in many of his views and judgments in the field of immunology, pathology, bacteriology, zoology and comparative embryology. Today Metchnikoff is deservedly called the father of the theory of cellular immunity, and also a harbinger of the theory of natural immunity. His work in the field of lactic acid bacteria formed the basis for an entire industry of probiotics. Metchnikoff’s doctrine of the possibility of extending human life is still relevant. This publication is one more attempt to tie the biography of the scientist with his creative legacy and his contribution in the world treasury of biological science
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Данилова В. М., Виноградова Р. П., Черниш І. Г., Петренко Т. М.
Заглавие : Винахідницька діяльність відділу регуляції обміну речовин Інституту біохімії ім. О. В. Палладіна НАН України
Параллельн. заглавия :Inventive activity of the Department of Metabolism Regulation of the Palladin Institute of Biochemistry of NAS of Ukraine
Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 6. - С. 110-121 (Шифр УУ1/2016/88/6)
Примечания : Бібліогр.: в кінці ст.
MeSH-главная: НАУЧНЫЕ ИССЛЕДОВАНИЯ -- RESEARCH
УКРАИНА -- UKRAINE
БИОХИМИЯ -- BIOCHEMISTRY
ДОСТИЖЕНИЕ -- ACHIEVEMENT
АКАДЕМИИ И ИНСТИТУТЫ -- ACADEMIES AND INSTITUTES
Аннотация: Статтю присвячено винахідницькій діяльності відділу регуляції обміну речовин Інституту біохімії ім. О. В. Палладіна НАН України в контексті історії його створення, розвитку, а також наукової діяльності його засновника, академіка НАН України М. Ф. Гулого, його учнів і послідовників. Побіжно йдеться про практичні здобутки М. Ф. Гулого, які виявилися такими самими вагомими, масштабними та різноманітними, як і наукові. Детальніше проаналізовано практичні результати наукових розробок його учнів і послідовників, які спрямовано на вирішення практичних завдань медицини, харчової промисловості, сільського господарства і які, по суті, є продовженням ідей і задумів М. Ф. Гулого
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Philchenkov A.
Заглавие : Caspases, calpains, granzymes, cathepsins, HTRAs: far more than just killer enzymes
Параллельн. заглавия :Каспази, калпаїни, гранзими, катепсини, HTRAs: більш, ніж тільки ензими-вбивці
Место публикации : Український біохімічний журнал. - 2016. - Т. 88, № 6. - С. 122-124 (Шифр УУ1/2016/88/6)
MeSH-главная: КНИЖНЫЕ ОБЗОРЫ КАК ТЕМА -- BOOK REVIEWS AS TOPIC
АПОПТОЗ-РЕГУЛИРУЮЩИЕ БЕЛКИ -- APOPTOSIS REGULATORY PROTEINS
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