Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Biochemical mechanisms of free-radical damage to the nuclear genome by cadmium / I. M. Trakhtenberg [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P5-16

MeSH-главная:
КАДМИЙ -- CADMIUM (токсичность)
ХРОМАТИН -- CHROMATIN (патология)
ДНК ПОВРЕЖДЕНИЕ -- DNA DAMAGE
Аннотация: Cadmium, has been used for many years in industry and agriculture. Its active use is associated with a danger to human health due to a significant toxic effect on the body. The purpose of the review was to analyze the literature data and the results of our own experimental research on the biochemical mechanisms of the toxic effect of cadmium on the nuclear chromatin of cells, primarily the liver. Based on this analysis, the concept that the toxicity of cadmium is associated with damage caused by cadmium-induced free radicals was confirmed. The reactive oxygen species formed in this way are active damaging agents that cause toxic effects, including mutagenic, which ultimately leads to apoptosis and cell death
Доп.точки доступа:
Trakhtenberg, I. M.
Gubsky, Y. I.
Levitsky, E. L.
Belenichev, I. F.

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Examining c-di-GMP and possible quorum sensing regulation in Pseudomonas fluorescens SBW25: links between intra- and inter-cellular regulation benefits community cooperative activities such as biofilm formation / O. V. Mochynets [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P17-31

MeSH-главная:
БИОПЛЕНКИ МИКРООРГАНИЗМОВ -- BIOFILMS
PSEUDOMONAS FLUORESCENS -- PSEUDOMONAS FLUORESCENS
ГУАНОЗИНМОНОФОСФАТ -- GUANOSINE MONOPHOSPHATE
Аннотация: Bacterial success in colonizing complex environments requires individual response to micro-scale conditions as well as community-level cooperation to produce large-scale structures such as biofilms. Connecting individual and community responses could be achieved by linking the intracellular sensory and regulatory systems mediated by bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) and other compounds of individuals with intercellular quorum sensing (QS) regulation controlling populations. There is growing evidence to suggest that biofilm formation by many pseudomonads is regulated by both intra and intercellular systems, though in the case of the model Pseudomonas fluorescens SBW25 Wrinkly Spreader in which mutations increasing c-di-GMP levels result in the production of a robust cellulose-based air-liquid interface biofilm, no evidence for the involvement of QS regulation has been reported. However, our recent review of the P. fluorescens SBW25 genome has identified a potential QS regulatory pathway and other QS–associated genes linked to c-di-GMP homeostasis, and QS signal molecules have also been identified in culture supernatants. These findings suggest a possible link between c-di-GMP and QS regulation in P. fluorescens SBW25 which might allow a more sophisticated and responsive control of cellulose production and biofilm formation when colonising the soil and plant-associated environments P. fluorescens SBW25 normally inhabits
Доп.точки доступа:
Moshynets, O. V.
Foster, D.
Karakhim, S. A.
McLaughlin, K.
Rogalsky, S. P.
Rymar, S. Y.
Volynets, G. P.
Spiers, A. J.

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The relationship between the ionized Ca concentration and mitochondrial functions / L. G. Babich [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P32-40

MeSH-главная:
МИТОХОНДРИИ ПЕЧЕНИ -- MITOCHONDRIA, LIVER
МИОМЕТРИЙ -- MYOMETRIUM
КАТИОНЫ -- CATIONS
КАЛЬЦИЙ -- CALCIUM
Аннотация: The aim of the study was to show the relationships between ionized Ca concentration ([Ca2+]m) in the mitochondria matrix and functional activity of this organelle. [Ca2+]m was determined using the fluorescent probe Fluo-4, AM. Total level of Ca2+ accumulation in mitochondria was monitored using 45Ca2+ as radioactive tracer. It was shown that incubation of myometrium mitochondria with 3 mM Mg2+ resulted in the low level of [Ca2+]m. Subsequent addition of 100 µM Ca2+ resulted in 8 times increase of [Ca2+]m but in low level of total calcium accumulation. Normalized fluorescence of Ca2+-sensitive probe Fluo-4 in response to the Ca2+ addition was higher than 2.5. At the same time, [Ca2+]m was considerably higher in the medium containing 3 mМ АТР and 3 mМ Mg2+. Subsequent addition of 100 µM Ca2+ to the incubation medium resulted in only 2.4 times increase of [Ca2+]m but considerably higher level of total calcium accumulation was observed. Normalized fluorescence of Fluo-4 in response to the Ca2+ addition was lower than 1.3. In liver mitochondria higher rate of oxygen consumption was detected in the presence of an oxidative substrate succinate than of pyruvate or α-ketoglutarate. At the presence of an oxidative substrate succinate normalized fluorescence of Fluo-4 in liver mitochondria in response to the Ca2+ addition was lower than 1.3. It was concluded that low level of [Ca2+]m was correlated with low functional activity of this organelle and, vise versa, high level of [Ca2+]m was correlated with high functional activity. It was suggested that normalized fluorescence changes in response to the Са2+ addition could be used as a test of the mitochondrial functional activity: lower normalized fluorescence values − higher functional activity
Доп.точки доступа:
Babich, L. G.
Shlykov, S. G.
Kushnarova-Vakal, A. M.
Kupynyak, N. I.
Manko, V. V.
Fomin, V. P.
Kosterin, S. O.

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Activation of store - operated Ca2+ entry in cisplatin resistant leukemic cells after treatment with photoexcited fullerene C60 and cisplatin / D. V. Franskevych [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P41-48

MeSH-главная:
КЛЕТОК ТРАНСФОРМАЦИЯ НЕОПЛАСТИЧЕСКАЯ -- CELL TRANSFORMATION, NEOPLASTIC
ЛЕКАРСТВЕННАЯ УСТОЙЧИВОСТЬ -- DRUG RESISTANCE
ЦИСПЛАТИН -- CISPLATIN
ФУЛЛЕРЕНЫ -- FULLERENES
Аннотация: Ca2+-regulating system in cancer cells is suggested to be remodulated particularly by reduced store-operated Ca2+ entry (SOCE) through plasma membrane in order to maintain moderately reduced cytosolic Ca2+ concentration and to avoid apoptosis. The endoplasmic reticulum (ER) Ca2+ pool content and the size of SOCE in leukemic wild type (L1210) and resistant to cisplatin (L1210R) cells in control, after treatment with either cisplatin (1 µg/ml) or photoexcited fulleren C60 (10-5 M) alone, or their combination were estimated with the use of Indo-1 AM. The SOCE in resistant to cisplatin L1210R cells was found to be lower than in the wild-type cells. After treatment with cisplatin the decrease of thapsigargin (TG)-sensitive ER Ca2+ pool with no significant increase of SOCE was observed in L1210 cells, while no changes were detected in L1210R cells. Photoexcitation of intracellular accumulated fullerene C60 in the visible range of spectrum (410-700 nm) was accompanied by increase of SOCE not only in sensitive, but in resistant cells as well. In resistant L1210R cells treated with photoexcited C60 essential effect of cisplatin on Ca2+ homeostasis became obvious: the size of SOCE proved to be higher than after treatment with photoexcited C60 alone. The data obtained allow suggesting the influence of photoexcited C60 not only on Ca2+-regulating system, but on those involved in controlling cisplatin entry into drug resistant cancer cells
Доп.точки доступа:
Franskevych, D. V.
Grynyuk, I. I.
Prylutska, S. V.
Matyshevska, O. P.

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ERN1 modifies the effect of glutamine deprivation on tumor growth related factors expression in U87 glioma cells / O. H. Minchenko [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P49-61

MeSH-главная:
ГЛУТАМИН -- GLUTAMINE (дефицит)
ГЕННАЯ ЭКСПРЕССИЯ -- GENE EXPRESSION
КЛЕТОЧНЫЕ ЛИНИИ НОВООБРАЗОВАНИЙ -- CELL LINE, TUMOR
Аннотация: The expression of a subset of genes encoding important tumor growth related factors in U87 glioma cells with ERN1 (endoplasmic reticulum to nucleus signaling 1) loss of function as well as upon glutamine deprivation was studied. It was shown that glutamine deprivation down-regulated the expression level of ATF6 (activating transcription factor 6), EIF2AK3/PERK (eukaryotic translation initiation factor 2 alpha kinase 3), GLO1 (glyoxalase I), BIRC5 (baculoviral IAP repeat-containing 5), and RAB5C (RAB5C, a member of RAS oncogene family) mRNAs in control glioma cells. At the same time, the expression level of HSPB8 (heat shock 22kDa protein 8) and HSPA5/GRP78 (heat shock protein family A (Hsp70) member 5) mRNAs was resistant to glutamine withdrawal in these glioma cells. It was also shown that inhibition of ERN1, which controled cell proliferation and tumor growth, modified the effect of glutamine deprivation on the expression levels of most studied genes in U87 glioma cells: up-regulated the expression of ATF6 and HSPA5 genes and enhanced sensitivity of EIF2AK3 and BIRC5 genes to glutamine withdrawal. Furthermore, the expression of all studied genes, except EIF2AK3, was down-regulated in ERN1 knockdown glioma cells in the presence of glutamine. It was demonstrated that glutamine deprivation affected the expression of most studied genes in ERN1 dependent manner and that these changes possibly contributed to the suppression of glioma growth from cells without ERN1 signaling enzyme function
Доп.точки доступа:
Minchenko, O. H.
Kharkova, A. P.
Hnatiuk, O. S.
Luzina, O. Y.
Kryvdiuk, I. V.
Kuznetsova, A. Y.

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Gerush, I. V.
The effect of melationin on lipid peroxide oxidation, oxidative modification of proteins and mitochondria swelling in the skeletal muscle tissue of rats under alloxan diabetes / I. V. Gerush, V. V. Bevzo, Ye. O. Ferenchuk // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P62-69

MeSH-главная:
МЕЛАТОНИН -- MELATONIN
ПЕРЕКИСНОЕ ОКИСЛЕНИЕ ЛИПИДОВ -- LIPID PEROXIDATION
МИТОХОНДРИЙ НАБУХАНИЕ -- MITOCHONDRIAL SWELLING
МЫШЦА СКЕЛЕТНАЯ -- MUSCLE, SKELETAL
ДИАБЕТ САХАРНЫЙ ЭКСПЕРИМЕНТАЛЬНЫЙ -- DIABETES MELLITUS, EXPERIMENTAL
Аннотация: The aim of the investigation was to study the effect of melatonin on of peroxide lipid oxidation, the oxidative modification of proteins and the mitochondria swelling in skeletal muscle tissue of rats under alloxan diabetes. The intensification of free radical processes in the mitochondria skeletal of muscle tissue of the diabetic rats was accompanied by an increase in the content of thiobarbiturate-active products and in the oxidation process of proteins mitochondria. It was manifested by the decrease in the content of free SH-groups and the accumulation of carbonyl derivatives. The light scattering of the suspension of mitochondria decreases that evidences for their swelling. So, the mitochondria swelling was found to be associated with the increase of thiobarbiturate-active products and the decreased content of free SH-groups in the mitochondrial fraction of the muscle tissue of diabetic rats. Melatonin has a corrective effect on the processes of free radical oxidation of lipids and proteins of mitochondrial fractions and on the intensity of swelling of mitochondria.
Доп.точки доступа:
Bevzo, V. V.
Ferenchuk, Ye. O.

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Development and characterization of highly informative elisa for the detection of IgG and IgA antibodies to Chlamydia trachomatis / O. Yu. Galkin [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P70-83

MeSH-главная:
ИММУНОФЕРМЕНТНЫЕ МЕТОДЫ -- IMMUNOENZYME TECHNIQUES
АНТИТЕЛА МОНОКЛОНАЛЬНЫЕ -- ANTIBODIES, MONOCLONAL
ИММУНОГЛОБУЛИН G -- IMMUNOGLOBULIN G
CHLAMYDIA TRACHOMATIS -- CHLAMYDIA TRACHOMATIS
Аннотация: The goal of this work was developing of highly informative an enzyme-linked immunosorbent assay (ELISA) for the detection of IgG and IgA antibodies against to Chlamydia trachomatis, as well as comparative characterization of developed assay using standardized control materials. The study was conducted using: monoclonal antibodies (McAbs) to human IgA and IgG; recombinant Ch. trachomatis proteins – Pgp3, major outer membrane protein (MOMP); two panels of characterized sera and four reference ELISA kits. The study of immunochemical activity of peroxidase conjugates of McAbs was performed in comparison with conjugates of commercial analogues: anti-IgG McAb 2A11 and anti-IgA McAb AD3. About half of the conjugates from the received McAbs panel were more active compared to the reference antibody conjugates. It was quite justified to use the conjugates of antibodies that interact with different antigenic determinants. When IgG antibodies were detected to MOMP, it was justified 1.14-1.56 times more; when IgA antibodies were detected to MOMP, it was justified 1.16-1.37 times more. ELISA for detecting IgG/IgA antibodies to MOMP and Pgp3 of Ch. trachomatis were evaluated using appropriately described serum panels OCO-42-28-313-00 and OCO-42-28-314-00. Comparative studies of the developed ELISA for the detection of IgG and IgA antibodies to the MOMP and Pgp3 of Ch. trachomatis showed their prominent advantage over the commercial analogues, which more clearly demonstrates the difference in the ratio of average values of optical density of positive and negative samples of the described panel of sera: this indicator for commercial kits was 1.36-3.59 times less
Доп.точки доступа:
Galkin, O. Yu.
Gorshunov, Yu. Y.
Besarab, O. B.
Ivanova, O. M.

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Ohiri, R. C.
Nutriceutical potential of Pleurotus tuber-regium sclerotium / R. C. Ohiri // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P84-93

MeSH-главная:
PLEUROTUS -- PLEUROTUS
МИКРОЭЛЕМЕНТЫ -- TRACE ELEMENTS
АМИНОКИСЛОТЫ -- AMINO ACIDS
ЛЕТУЧИЕ ОРГАНИЧЕСКИЕ СОЕДИНЕНИЯ -- VOLATILE ORGANIC COMPOUNDS
ФАРМАКОГНОЗИЯ -- PHARMACOGNOSY
Аннотация: The aim of the study was to determine the composition of the sclerotium of Pleurotus tuber-regium and to analyze its nutritional potential. Major minerals and micronutrients content of the P. tuber-regium sclerotium were determined. The study has shown fairly high concentrations of potassium and magnesium as major minerals with values of 60.66 ± 4.13 and 41.79 ± 3.14 mg/kg, while manganese and zinc were micronutrients with the highest values of 1.20 ± 0.10 and 0.95 ± 0.07 mg/kg. Glutamic acid and aspartic acid were also observed in high concentrations with values of 11.51 ± 1.01 and 5.52 ± 0.86 mg/kg. The mushroom powder of P. tuber-regium was a source for production of oil, which was analyzed by GC-MS method. Benzenedicarboxylic acid mono-(2-ethylhexyl) ester and benzenedicarboxylic acid butyl-cyclohexyl ester were volatile constituents predominating with percentage total of 78.7 and 5.2, respectively. It is concluded that the presence of mineral elements, amino acids and volatile components observed in this fungus indicated the presence of the nutritional potential in the sclerotia of P. tuber-regium
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Horak, I. R.
Adaptor protein Ruk/CIN85 modulates resistance to doxorubicin of murine 4T1 breast cancer cells / I. R. Horak, D. S. Gerashchenko, L. B. Drobot // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P94-100

MeSH-главная:
МОЛОЧНОЙ ЖЕЛЕЗЫ ЖИВОТНЫХ НОВООБРАЗОВАНИЯ -- MAMMARY NEOPLASMS, ANIMAL
АДАПТЕРНЫЕ БЕЛКИ ВЕЗИКУЛЯРНОГО ТРАНСПОРТА -- ADAPTOR PROTEINS, VESICULAR TRANSPORT
ЛЕКАРСТВЕННАЯ УСТОЙЧИВОСТЬ -- DRUG RESISTANCE
Аннотация: The acquisition of chemoresistance in the course of tumor progression includes activation of membrane ABC transporters, detoxification enzymes, cell cycle deceleration and activation of specific signaling pathways such as Akt/mTOR, MAPK, NF-κB. Adaptor proteins play an essential role in the assembly of supramolecular signaling complexes, maintaining and directing the intracellular signaling. One of such proteins, called Ruk/CIN85, is strongly associated with malignant transformation and metastasis. In present study we investigated the Ruk/CIN85 effect of up/down-regulation on the transforming potential and doxorubicin resistance of highly aggressive mouse breast adenocarcinoma 4T1 cells. It was demonstrated that 4T1 cells overexpressing Ruk/CIN85 possessed increased resistance to doxorubicin (in the range of concentrations 0.1–10.0 µM) while knockdown cells were the most sensitive. Also, high levels of Ruk/CIN85 in 4T1 cells positively correlated with their ability to form colonies in semi-solid agar. Ruk/CIN85-overexpressing cells formed four times more colonies in comparison with Ruk/CIN85 nockdown cells, the growth of which revealed higher resistance to doxorubicin action
Доп.точки доступа:
Gerashchenko, D. S.
Drobot, L. B.

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10.

Modern aspects of biochemistry and biotechnology - 2018 : young scientists conference dedicated to the 100th Anniversary of the National Academy of Sciences of Ukraine, May 23-25, 2018, Kyiv // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P101-147

MeSH-главная:
БИОХИМИЯ -- BIOCHEMISTRY
БИОТЕХНОЛОГИЯ -- BIOTECHNOLOGY
КОНГРЕССЫ -- CONGRESSES
Доп.точки доступа:
Modern aspects of biochemistry and biotechnology - 2018, young scientists conference dedicated to the 100th Anniversary of the National Academy of Sciences of Ukraine

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11.

Cellular plasticity as a driving force in cancer progression: the regulatory role of adaptor protein RuK/CIN85 / I. R. Horak [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P102-103

Доп.точки доступа:
Horak, I. R.
Pasichnyk, G. V.
Geraschenko, D. S.
Knopfova, L.
Borsig, L.
Drobot, L. B.

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12.

Modulating effect of cholecalciferol on vitamin D endo/para/autocrine system in type 1 diabetes / A. O. Mazanova [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P104-105

Доп.точки доступа:
Mazanova, A. O.
Lisakovska, O. O.
Makarova, O. О.
Shymanskyi, I. O.

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13.

Uspenska, K. R.
Biological role of nicotinic acetylcholine receptors in mitochondria / K. R. Uspenska // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P106

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14.

G-quadruplex DNA binding and telomerase inhibition by pheophorbides / A. M. Chukova [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P107

Доп.точки доступа:
Chukova, A. M.
Negrutska, V. V.
Saraieva, I. V.
Dubey, L. V.

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15.

Demenko, D.
Computer modeling of direct factor Xa inhibitors / D. Demenko, V. Hurmach, V. Chernyshenko // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P108

Доп.точки доступа:
Hurmach, V.
Chernyshenko, V.

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16.

The effect of N-stearoylethanolamine on age-related and diet-induced changes of fatty acid profile of rat adipocytes / O. S. Dziuba [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P109

Доп.точки доступа:
Dziuba, O. S.
Polishchuk, I. V.
Kosiakova, H. V.
Klimashevsky, V. M.

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17.

Garmash, I. A.
Regulation of glycolysis-related genes expression in U87 ERN1 knockdown glioma cells / I. A. Garmash // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P110

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18.

Deficiency of adaptor protein RuK/CIN85 in lewis lung carcinoma cells inhibits malignancy hallmarks in vitro as well as tumor growth and pulmonary metastasis in vivo / D. S. Gerashchenko [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P111

Доп.точки доступа:
Gerashchenko, D. S.
Shytikov, D. V.
Horak, I. R.
Khudiakova, O. V.
Skaterna, T. D.
Drobot, L. B.

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19.

Gorshkova, O. G.
Effective method for cleaning of ore of ore-ferrous factory from ions of heavy metals based on bacteria association of the genus Pseudomonas / O. G. Gorshkova // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P112

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20.

Gritsenko, M.
Analysis of the expression and products accumulation of CD-marker genes in cultures of lung and skin rats fibroblasts in ontogenesis / M. Gritsenko // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 3. - P113

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