Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Pattern of expression of immune- and stroma-associated genes in blood of mice with experimental B16 melanoma / G. V. Geraschchenko [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 1. - P5-11

MeSH-главная:
МЕЛАНОМА ЭКСПЕРИМЕНТАЛЬНАЯ -- MELANOMA, EXPERIMENTAL (иммунология, кровь)
Аннотация: The interaction between malignant and stromal cells represents a major cross-talk pathway upon carcinogenesis. Cellular elements of the reactive tumor stroma are a heterogeneous population which are represented specifically by cancer-associated fibroblasts (CAFs) and tumor-associated macrophages (TAM). It is not known whether expression of CAF- and TAM-associated genes could be detected in the peripheral blood of cancer patients to monitor a course of disease. The aim of the study was to assess the relative expression (RE) of cancer-related genes in peripheral blood of mice with experimental melanoma. Quantitative PCR was used to determine RE of 15 genes in the blood of C57BL/6j control mice and mice with injected B16 melanoma cells. The Kruskal-Wallis and the Fischer exact tests with correction on multiple comparisons, according to the Benjamini-Hochberg procedure with FDR = 0.2 were used for statistical analysis. Analysis of 15 immune and stromal markers RE showed differentiated expression of several CAF and TAM markers in mice with experimental melanoma in comparison with the control animals. Thus, CAF markers Acta2, Cxcl14, Fap and TAM markers Cd68, Ccl22 and Ccl17 were significantly upregulated, while Cd4, Cd3 were downregulated. This, together with increased expression of Cox-2 suggested a stable immunosuppressive state of mice with experimental melanomas. The results of the study showed that potential markers of cancer-associated fibroblasts and tumor-associated macrophages in peripheral blood of mice with experimental melanoma could be used for non-invasive detection of melanoma cell progression
Доп.точки доступа:
Geraschchenko, G. V.
Vagina, I. M.
Vagin, Yu. V.
Kashuba, V. I.

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Proline denydrogenase (prodh) gene polymorphisms and the risk of schizophrenia in iranian populations / F. H. Moghadam [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 1. - P12-20

MeSH-главная:
ШИЗОФРЕНИЯ -- SCHIZOPHRENIA (этиология)
ПОЛИМОРФИЗМ ГЕНЕТИЧЕСКИЙ -- POLYMORPHISM, GENETIC (генетика, иммунология)
Аннотация: Schizophrenia is a highly heritable mental disorder which can be occurred as a result of mutations or single nucleotide polymorphisms (SNPs) in various genes. Proline dehydrogenase (PRODH) gene is one of the most important genes which can be associated with increased risk of schizophrenia in several populations. Here, we considered the effect of PRODH gene polymorphisms on the incidence of schizophrenia in Iranian populations. This study was done using the analysis of 3 SNPs markers, including G1496A, G758A and C1482T. Molecular analysis was performed on 263 schizophrenic patients and 278 healthy individuals (control group). These examinations were executed by PCR-based restriction fragment length polymorphism (RFLP) technique. Statistical analysis was performed by SPSS software (16.0). Our findings showed that G1496A and C1482T polymorphisms in patients were significantly higher than controls and there were meaningful correlations between the occurrence of these polymorphisms and schizophrenia in the population (P 0.001). However, there was no significant relationship between G758A in the PRODH gene and schizophrenia. Haplotype analysis showed that AAT, AAC and GAT blocks (variation alleles are bold) had significant correlations with schizophrenia. PRODH gene can be considered as one of the important genes involved in schizophrenia development among the Iranian population
Доп.точки доступа:
Moghadam, F. H.
Mehrabani, Z. H. A.
Amounajaf, M.
Rahmanzadeh, S.
Ghasemvand, F.
Samghabadi, A. S.
Nejadmoghaddam, A.
Omidinia, E.

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Inhibition of Na(+),K(+)-ATPase and activation of myosin ATPase by CALIX[4]A RENE C-107 cause stimulation of isolated smooth muscle contractile activity / T. O. Veklich [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 1. - P21-30

MeSH-главная:
МЫШЦЫ -- MUSCLES (действие лекарственных препаратов)
Аннотация: The discovery of compounds that might modify myometrial contractility is an important area of researches. In our previous experiments, we found that some representatives of macrocyclic compounds family – calix[4]arenes – can modify the enzymatic and transport activity of membrane-bound cation-transport ATP hydrolases. The aim of this work was to study and compare the effect of calix[4]arene C-107 on the enzymatic activities of Mg2+-dependent ATPases of the uterine smooth muscle, namely: ouabain-sensitive Na+,K+-ATPase, plasma membrane Ca2+-independent “basal” Mg2+-ATPase, ATPase of the actomyosin complex and myosin subfragment-1, with effect on the contractile activity of the myometrium. It was shown that calix[4]arene C-107 efficiently inhibited myometrium Na+,K+-ATPase (I50 = 54 ± 6 nM) selectively to other ATP-hydrolases of the plasma membrane and simultaneously activated the enzymatic activity of the myosin ATPase of smooth muscles (A50 = 9.6 ± 0.7 μM). Such reciprocal biochemical effects led to the stimulation of the smooth muscle contractile activity that was demonstrated by the tensometric method using different isolated smooth muscles. Calix[4]arene С-107 was shown to stimulate the increase of the tonic component of myometrium contractions induced by oxytocin, as well as contractions of the caecum muscles induced by high-potassium solution or acetylcholine, and to maintain increased tension for a long time. Thus, calix[4]arene C-107 is a prospective compound for enhancing the smooth muscle basal tone and/or contraction in case of hypotonic dysfunctions
Доп.точки доступа:
Veklich, T. O.
Labyntseva, R. D.
Shkrabak, O. A.
Tsymbalyuk, O. V.
Rodik, R. V.
Kalchenko, V. I.
Kosterin, S. O.

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Tykhomyrov, A. A.
Plasminogen modulates formation and release of platelet angiogenic regulators / A. A. Tykhomyrov, D. D. Zhernosekov, T. V. Grinenko // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 1. - P31-40

MeSH-главная:
НЕОВАСКУЛЯРИЗАЦИЯ ФИЗИОЛОГИЧЕСКАЯ -- NEOVASCULARIZATION, PHYSIOLOGIC (иммунология)
АНГИОСТАТИНЫ -- ANGIOSTATINS (иммунология)
Аннотация: Platelets store, produce and release a variety of angiogenesis regulators, which can contribute to both normal tissue repair and angiopathy-associated pathologies. Plasminogen has been earlier shown to regulate some platelet functions, but if it is able to modulate angiogenic capacities of platelets is still poorly studied. Thus, the aim of the present study was to evaluate the effects of different plasminogen forms on the formation and secretion of angiogenic protein regulators by platelets. Human washed platelets were obtained by gel-filtration on Sepharose-2B. The levels of P-selectin (CD-62P) exposed on the plasma membrane of untreated and activated platelets was monitored by flow cytometry. Secretion of platelet-derived vascular endothelial growth factor (VEGF) as well as plasminogen fragmentation and angiostatin formation by intact platelets and platelet plasma membranes were analyzed by immunoblotting. It was shown that thrombin or collagen exposure resulted in enhanced P-selectin surface expression by platelets, while Lys-form of plasminogen reduced agonist-induced platelet secretion. Lys-plasminogen, but not Glu-form, inhibited agonist-induced VEGF release from platelets. Activation of platelets significantly accelerated plasminogen cleavage and angiostatin formation. Anti-actin antibodies inhibited plasminogen fragmentation during incubation with platelet plasma membranes indicating surface-exposed actin participation in plasminogen conversion to angiostatins. The present study uncovers a novel function of plasminogen to limit angiogenic potential of platelets via angiostatin formation and inhibition of VEGF secretion
Доп.точки доступа:
Zhernosekov, D. D.
Grinenko, T. V.

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Influence of Tl(+) on the Ca(2+) and Na(+) movement across rat neonatal cardiomyocytes and rat heart mitochondria membranes / S. M. Korotkov [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 1. - P41-55

MeSH-главная:
МИОЦИТЫ СЕРДЕЧНЫЕ -- MYOCYTES, CARDIAC (иммунология)
МИТОХОНДРИИ -- MITOCHONDRIA (иммунология)
Аннотация: Thallium is known to produce one of the most complex and serious patterns of toxicity, involving a wide range of human organs and tissues. The toxic impact on biologic organisms is linked especially to the ability of Tl+ to disturb calcium homeostasis and to permeate easily the inner mitochondrial membrane (IMM). The aim of this work was to study the effects of Tl+ on intracellular Ca2+ dynamics in rat neonatal cardiomyocytes as well as on sodium penetrability of the IMM and Tl+-induced mitochondrial permeability transition pore (MPTP) opening in isolated Ca2+-loaded rat heart mitochondria (RHM). The use of the fluorescent calcium indicator Fura 2 AM showed that Tl+ induced calcium influx across the plasmatic membrane, resulting in calcium ([Ca2+]i) increase in the cytoplasm. This increase was even more pronounced in experiments with accelerating of Tl+-transmembrane fluxes by nonactin. It was nevertheless abolished by the removal of extracellular Ca2+ ions, but was not inhibited by a calcium-channel blocker (nifedipine). Tl+ did not release calcium from the intracellular stores. Tl+ potentiated sodium permeability of the IMM because swelling of nonenergized RHM in medium containing TlNO3 and NaNO3 was enhanced at high Tl+ concentration. The calcium load of RHM induced MPTP opening which was accompanied by the increase of the swelling as well as the decrease of the inner membrane potential and of state 40 (basal) and state 3UDNP (2,4-dinitrophenol-uncoupled) respiration. These effects of Tl+ were suppressed by MPTP inhibitors (cyclosporine A, ADP and n-ethylmaleimide). The data obtained showed that Tl+-stimulated influx of extracellular calcium into cardiomyocytes could cause calcium and sodium RHM overload, which lead to the MPTP opening, thus determining the sensitivity of heart muscle to thallium intoxication
Доп.точки доступа:
Korotkov, S. M.
Nesterov, V. P.
Belostotskaya, G. B.
Brailovskaya, I. V.
Novozhilov, A. V.
Sobol, C. V.

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Profiling of metabolic biomarkers in the serum of prostate cancer patients / F. Ali [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 1. - P56-65

MeSH-главная:
ПРЕДСТАТЕЛЬНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- PROSTATIC NEOPLASMS (иммунология, кровь, метаболизм)
Аннотация: Prostate cancer (PCa) is the major cause of the death of men population globally. Multiple factors are involved in the initiation and progression of PCa. This study aimed to evaluate different metabolic parameters in the serum of PCa patients. Males of 50 years and above age with the recent diagnosis of PCa (digital rectal examination, and elevated serum prostate-specific antigen (PSA) level) were included in the study. Glucose and serum electrolytes level, lactate dehydrogenase activity, parameters of lipid metabolism and liver and kidney functioning were measured on a fully automated analyzer using standard reagent kits. Oxidative stress was evaluated by measuring MDA, CAT, GSH, and SOD in serum. Detection of C-reactive protein (CRP), insulin-like growth factor (IGF-1) and vascular endothelial growth factor (VEGF) was performed by immunoassay. It was shown that serum glucose and HDL levels were lower while total cholesterol, LDL and triglyceride levels were significantly higher in PCa group than in the control group. PCa patients had an elevated level of liver and kidney functional markers. Comparison of the oxidative stress markers in patient and control groups showed significant difference. It was detected that serum levels of CRP, IGF-1 and VEGF were significantly higher in PCa group, compared the control to group (P 0.05). Low level of glucose and dyslipidemia indices in prostate cancer patients indicated metabolic changes and demonstrated the importance of multiple parameters analysis (free PSA, dyslipidemia, VEGF, IGF-1, CRP, and oxidative stress markers) for early PCa diagnostics
Доп.точки доступа:
Ali, F.
Akram, S.
Niaz, S.
Wajid, N.

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Methotrexate effect on biochemical indices of psoriasis patients depends on MTHFR gene polymorphism / O. M. Fedota [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 1. - P66-74

MeSH-главная:
ПСОРИАЗ -- PSORIASIS (генетика, кровь, лекарственная терапия)
МЕТОТРЕКСАТ -- METHOTREXATE (терапевтическое применение, фармакокинетика)
Аннотация: Methotrexate (MTX) is the immunosuppressive anti-inflammatory drug and the antagonist of the enzyme dihydrofolate reductase. Pharmacogenomic studies and clinical evidences suggest that altered response to MTX in patients with different diseases is associated with polymorphisms of genes that regulate folate metabolism. The purpose of the article was to analyze the methotrexate effect on the biochemical indices of psoriasis patients depending on methylenetetrahydrofolate reductase gene (MTHFR) polymorphisms. Effects of two single-nucleotide polymorphisms, C677T and A1298C, were studied. An increase of alanine aminotransferase and aspartate aminotransferase activity above the normal level in the patients with both MTHFR gene polymorphisms after methotrexate intake was observed. In patients with CC, TT, CT genotypes for C677T polymorphism and AA genotype for A1298C polymorphism of MTHFR gene, significant differences in alpha-amylase activity before and after treatment with methotrexate were detected. Analysis of the biochemical indices of patients with arthropathic and vulgaris psoriasis showed that the positive effect of MTX treatment could be associated with wild-type alleles in both polymorphisms of MTHFR gene, while the ineffectiveness of methotrexate was associated with the dihеterozygous genotype. The largest number of smokers was found within the CTAA genotype group (37.5%), while no smokers were observed within TTAA patients and most of CCAA patients. The data obtained testify the utility of the individual approach to the psoriasis patients therapy taking into account genetic background
Доп.точки доступа:
Fedota, O. M.
Roschenyuk, L. V.
Tyzhnenko, T. V.
Puzik, N. G.
Vorontsov, V. M.
Ryzhko, P. P.

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Indexes of nitric oxide system in experimental antiphospholipid syndrome / O. Z. Yaremchuk [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 1. - P75-83

MeSH-главная:
АНТИФОСФОЛИПИДНЫЙ СИНДРОМ -- ANTIPHOSPHOLIPID SYNDROME (кровь, метаболизм, патофизиология)
Аннотация: Antiphospholipid syndrome (APS) is an autoimmune disease characterized by the presence of antibodies to negatively charged membrane phospholipids (aPL). Endothelial dysfunction is one of the most dangerous APS manifestations followed by thrombosis, placental insufficiency and often foetal death due to circulatory disorders in placenta blood vessels. It is established that synthesis and bioavailability of nitric oxide (NO) in the endothelium are impaired at APS, but the role of NO system in pregnancy failure at this pathology remains ambiguous. The aim of this research was to estimate the indexes of the nitric oxide system in animals with an experimental antiphospholipid syndrome before pregnancy and on the 18th day of pregnancy, without treatment and under treatment with nitric oxide synthesis modulators (L-arginine and aminoguanidine). In the blood serum and liver of the BALB/c mice with experimental APS, the content of eNOS and iNOS– by ELISA and the level of NO2– and NO3– with the use of Gris reagent were determined before pregnancy and on the 18th day of pregnancy. The data obtained indicate the relative inefficient NO production by eNOS and NO hyperproduction by iNOS in the blood serum and liver of mice in the pathogenesis of experimental APS. Thus, in mice with APS before pregnancy and on the 18th day of the pregnancy, the eNOS content and NO2– level were decreased while the iNOS content and NO3– level were increased compared to the indexes in the control animal group. L-arginine administration to the animals with APS at the follow-up periods resulted in an increased eNOS content and NO2–, NO3– levels in blood serum and liver with the simultaneous decrease in iNOS content in the liver as compared to indexes in untreated mice with APS. The combined use of L-arginine and selective iNOS inhibitor aminoguanidine caused a significant increase in eNOS content and a decrease in iNOS content followed by normalization of NO2– and NO3– levels in blood and liver of mice with experimental APS before pregnancy and on the 18th day of pregnancy compared to untreated mice with APS
Доп.точки доступа:
Yaremchuk, O. Z.
Posokhova, K. A.
Kuzmak, I. P.
Kulitska, M. I.
Klishch, I. M.
Korda, M. M.

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Phenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strain / T. V. Gudzenko [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 1. - P84-91

MeSH-главная:
BREVIBACILLUS -- BREVIBACILLUS (выделение и очистка, рост и развитие)
СТОЧНЫЕ ВОДЫ -- WASTE WATER (анализ, микробиология)
Аннотация: Biotechnological treatment of enterprises wastewater, which contains toxic phenolic compounds, is the most acceptable method today. Therefore, the search for new non-pathogenic biochemically active microorganisms – destructors of phenol and their identification is relevant. The aim of this study was to determine phenol-oxidizing activity and the fatty acid profile of Brevibacillus centrosporus F14 – destructor of phenol, isolated from pharmaceutical plant wastewater. Fatty acids analysis of the microorganism culture was carried out using automatic system of microorganisms identification MIDI Sherlock based on gas chromatograph Agilent 7890. The phenol-oxidizing activity of bacteria was determined by photometric method according to the degree of phenol extraction from the water. It was experimentally confirmed that Brevibacillus sp. F14 strain had a phenol-destructive activity. When phenol-containing water was treated with Brevibacillus sp. F14 cells, phenol concentration in the water decreased from 200.0 ± 12.0 mg/l to 6.8 ± 0.8 mg/l during 15 days of exposure. Chromatographic analysis showed that saturated fatty acids i-14:0 (14.9%), i-15:0 (14.8%), a-15:0 (34.9%), i-16:0 (11.10%) dominated in the strain’s fatty acids profile. The total content of the branched saturated fatty acids i-14:0, i-15:0, a-15: 0, i-16: 0, i-17: 0, a-17:0, ∑(a-17:1/i-17:1) was 85.0% and that of saturated and unsaturated fatty acids with the normal structure – 7% of the total fatty acids pool. The distinction of Brevibacillus centrosporus F14 strain’s fatty acid profile from other bacteria of Brevibacillus genus was the presence of more i-14:0 (14.85%), i-16:0 (11.10%) fatty acids and 16: 1 w7c alcohol (7.71%), as well as the minor content of fatty acids markers 16:0 N alcohol (0.60%), 18:3 w6c (0.4%) and ∑(a-17:1/i-17:1) (1.94%). According to the phenotypic characteristics and fatty acid composition, the tested phenol-oxidizing strain belongs to Brevibacillus centrosporus species. The index of Brevibacillus centrosporus F14 strain’s fatty acid profile similarity with the library was 0.645
Доп.точки доступа:
Gudzenko, T. V.
Voliuvach, O. V.
Gorshkova, O. G.
Ostapchuk, A. M.
Ivanytsia, V. O.

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10.

Production and physicochemical characterization of xanrhan gum by native lactose consuming isolates of Xanthomonas citri subsp. citri / R. Moravej [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 1. - P92-102

MeSH-главная:
XANTHOMONAS VESICATORIA -- XANTHOMONAS VESICATORIA (физиология)
ЛАКТОЗА -- LACTOSE (метаболизм)
БИОПОЛИМЕРЫ -- BIOPOLYMERS (биосинтез)
Аннотация: Xanthan is a biopolymer produced by Xanthomonas bacteria which is widely used in many industries such as food and oil. In this work, three Xanthomonas strains (X. citri/NIGEB-88, X. citri/NIGEB-386 and X. citri/NIGEB-K37) were used to evaluate their industrial potential to produce xanthan gum in whey medium. Bacteria growth rate, viscosity, biomass, dry weigh of produced xanthan and β-galactosidase activity were studied during the fermentation process and the presence of β-galactosidase genes was assessed by PCR technique. Strain NIGEB-386 had the best ability to utilize lactose in the whey medium. The highest amount of xanthan production and viscosity were 22.7 g/l and 2066.6 mPa·s, respectively. The presence of six β-galactosidase genes in strains NIGEB-386 and NIGEB-K37 was confirmed. The pyruvate and acetyl contents in xanthan gum were 2.1 and 0.29 %, respectively. Fourier-transform infrared spectroscopy analysis determined the position of the functional groups in the structure of the fermentation product. In whey medium, the performance of both NIGEB-386 and NIGEB-K37 strains were better than the X. campestris. The findings showed that Xanthomonas citri/NIGEB-386 is suitable for industrial production of xanthan using cheese whey as a low-cost medium
Доп.точки доступа:
Moravej, R.
Alavi, S. M.
Azin, M.
Salmanian, A. H.

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11.

Biochemical indicators of green photosynthetic bacteria Chlorobium limicola response to Cu2+ action / T. B. Sehin [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 1. - P103-112

MeSH-главная:
КЛЕТКИ ФИЗИОЛОГИЧЕСКИЕ ФЕНОМЕНЫ -- CELL PHYSIOLOGICAL PHENOMENA (действие лекарственных препаратов)
КСЕНОБИОТИКИ -- XENOBIOTICS (токсичность)
Аннотация: Photolithotrophic sulfur bacteria are involved in biota functioning and have a biotechnological potential for bioremediation of contaminated environment, but the mechanisms of xenobiotics, in particular of heavy metal ions damaging action and the pathways of photolithotrophic bacteria adaptation under these conditions have not been established. In this work, the biochemical indicators of green photosynthetic bacteria Chlorobium limicola response to Cu ions were studied. C. limicola cells were incubated during one hour in buffer containing copper (II) sulfate in 0.05–0.5 mM concentrations and grown for 8 days in GSB medium. The content of Cu2+ in cells was estimated by atomic absorption spectroscopy. The activity of enzymes of antioxidant defense, photosynthetic pigments and glutathione content, indexes of lipids unsaturation and membrane viscosity as markers of membrane fluidity were estimated. It was shown that the response of green photosynthetic bacteria C. limicola to Cu2+ action varied depending on cations concentration. Under the influence of metal salt at 0.05 mM concentration, the activity of antioxidant enzymes, GSH/GSSG ratio, the content of photosynthetic pigments and membrane fluidity indexes were higher as compared with control. Under the increase of copper (II) sulfate concentration to 0.25 mM, the activity of antioxidant enzymes was lower compared to the response of the cells under the influence of 0.05 mM copper (II) and the GSSG content was increased. Under the influence of 0.5 mM copper (II) the indexes of membrane fluidity did not differ from the control, but superoxide dismutase and peroxidase activity inhibition and the further decrease of GSH/GSSG ratio were observed followed by the highest Cu2+ cations accumulation in cells and significant decrease of bacteria biomass growth
Доп.точки доступа:
Sehin, T. B.
Hnatush, S. O.
Maslovska, O. D.
Halushka, A. A.
Zaritska, Y. H.

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12.

Kopylchuk, H. P.
Indexes of citrulline metabolism in rat liver under the toxic injury against the background of alimentary protein deficiency / H. P. Kopylchuk, I. M. Nykolaichuk, I. S. Lysyk // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 1. - P113-119

MeSH-главная:
ПЕЧЕНИ БОЛЕЗНИ -- LIVER DISEASES (метаболизм)
ЭУКАРИОТЫ -- EUKARYOTA
Аннотация: It is known that citrulline is converted into arginine in the series of metabolic transformations. Results of our previous studies showed that acetaminophen-induced toxic injury on the background of the alimentary deprivation of protein is accompanied by a decrease in arginine level in rat hepatocytes, but citrulline liver metabolism at these conditions remains incompletely clear. In this work, the content of citrulline in the rat liver mitochondrial and cytosolic fractions and the activity of citrulline-degrading enzymes – argininosuccinate synthase and argininosuccinate lyase were investigated. It was found that in the mitochondrial fraction a maximal reduction of the citrulline levels occurred after administration of acetaminophen toxic doses regardless of the protein amount in the ration, while in the cytosolic fraction the alimentary protein deficiency was a key factor in decreasing the activity of argininosuccinate synthase and arginino-succinate lyase. The data obtained indicated the disturbances of the urea cycle functioning and explained the decrease of L-arginine level in hepatocytes in conditions of acetaminophen-induced toxic injury against the background alimentary protein deficiency
Доп.точки доступа:
Nykolaichuk, I. M.
Lysyk, I. S.

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13.

Cytotoxic activity of the cluster rhenium compound with β-alanine ligands / K. V. Polokhina [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 1. - P120-126

MeSH-главная:
ЛЕЙКОЗЫ -- LEUKEMIA (лекарственная терапия)
РЕНИЙ -- RHENIUM (терапевтическое применение, фармакология)
Аннотация: Earlier we have shown that cluster rhenium compounds not only inhibited tumor growth in vivo but also supported the antioxidant state of experimental animals. Further investigation of new dirhenium(III) and cluster rhenium compounds in human leukemic cells is of great importance. The aim of the recent work was to investigate the cytotoxic activity of the new cluster rhenium compound with β-alanine ligands [Re2Cl6(C3H7NO2)2]·1.5H2O (I) in the solutions and nanoliposomes alone and together with cisplatin in Jurkat cells. It was shown that I in solution had cytotoxicity close to cisplatin (LC50 = 2.06·10-6 M). The administration of the rhenium-platinum system with І showed increased cytotoxic activity, especially high when both components of the system were in the mixed liposomes together (LC50 = 4.93·10-10 M). The new dirhenium dicarboxylate complex with zwitterionic amino acid ligands possesses an appreciable cytotoxic and proapoptotic activity against leukemic cells, especially in combination with cisplatin, guiding the search for novel active rhenium compounds and development of improved regimens for combined chemotherapy based on combination of rhenium-platinum compounds
Доп.точки доступа:
Polokhina, K. V.
Kytova, D. E.
Shtemenko, A. V.
Shtemenko, N. I.

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14.

Ketsa, O. V.
Free radical oxidation in liver mitochondria of tumor-bearing rats and its correction by essential lipophilic nutrients / O. V. Ketsa, M. M. Marchenko // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 1. - P127-134

MeSH-главная:
ПЕЧЕНЬ -- LIVER (действие лекарственных препаратов)
НУТРИЦИОННАЯ ТЕРАПИЯ -- NUTRITION THERAPY (использование)
Аннотация: The role of free radical oxidation in the increase of mitochondrial membranes permeability in organs which are not involved in oncogenesis and the development of the methods for preventing mitochondria dysfunction remain topical problems. In this work, the interconnection of lipid peroxidation (LPO) in liver mitochondrial fraction with the processes of mitochondrial swelling and cytochrome с release to the cytosol under separate and combined administration of ω-3 polyunsaturated fatty acids (PUFAs) and retinol acetate (vitamin A acetate) to rats with transplanted Guerin’s carcinoma was studied. During the intensive tumor growth (14 days) the increase of superoxide radical generation and the content of primary (triene conjugates, TC), secondary (ketodienes and coupled trienes, CD+CT) and terminal (Schiff bases) lipid peroxidation products in the mitochondrial fraction of tumor-bearing rats was detected, which contributed to the mitochondrial swelling and cytochrome с release to the cytosol. Separate administration of ω-3 PUFAs to tumor-bearing rats decreased both free radical processes in mitochondrial fraction and mitochondrial swelling. Separate administration of retinol acetate in a high dose (3000 IU/kg of body weight) intensified free radical processes in the mitochondrial fraction of tumor-bearing rats, while administration of retinol acetate in a physiological dose (30 IU/kg of body weight) did not lead to changes compared to tumor-bearing rats that did not receive the drug. The prooxidant effects of retinoid were partially eliminated in the case of combined administration with ω-3 PUFA
Доп.точки доступа:
Marchenko, M. M.

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15.

Виноградова, Р. П.
Внесок Нобелівських лауреатів в дослідження метаболізму вуглеводів і його регуляцію. А. Гарден, Х. Ейлер-Гельпін, К. Ф. Корі, Г. Т. Корі, Е. Сазерленд, Л. Ф. Лелуар, Г. Кребс, Ф. Ліпман, П. Мітчелл / Р. П. Виноградова, В. М. Данилова, С. В. Комісаренко // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 1. - С. 135-163

MeSH-главная:
НОБЕЛЕВСКАЯ ПРЕМИЯ -- NOBEL PRIZE
УГЛЕВОДНЫЙ ОБМЕН -- CARBOHYDRATE METABOLISM
Аннотация: Carbohydrate metabolism is a complex and multi-stage process. Many scientists (biochemists, physiologists, chemists) worked on deciphering this process, but only some of them were awarded the Nobel Prize. Thus, in the early XXth century, the work of A. Garden and H. Euler-Chelpin with yeast cells revealed that the conversion of carbohydrates (sugars) into end products occurs in living cells in several steps with the involvement of enzymes and that this conversion requires the presence of phosphoric acid residue. These studies were the beginning of exploring the chemical reactions (the reactions of intermediate metabolism) that are fundamentals for the vital functions of cells. In 1932, Hans Krebs discovered the ornithine cycle, a sequence of chemical reactions, in which the end product of nitrogen metabolism, urea, is formed in the liver of animals. The apogee of his research was the discovery of tri- and dicarboxylic acid cycle, which combines the oxidation of almost all organic compounds in living organisms. Fritz Lipmann, who in 1945 discovered coenzyme A and identified its role in the activation of organic compounds, furthered the works of H. Krebs. At the time, it became clear how inactive acetic acid and other organic acids are activated in living organisms to be oxidized in the tricarboxylic acid cycle. The great work was done by the spouses Gerty and Carl Cori, and Bernardo Houssay, as well as their students and followers, in particular, Luis Leloir to clarify the mechanism of conversion (synthesis and breakdown) of glycogen in the liver and muscles. The peak of studying the carbohydrate metabolism was the research of Earl Sutherland who in 1958 revealed the regulation of the activity of enzymes involved in the conversion of chemical compounds (the example – phosphorylase), with the participation of the adenylate cyclase enzyme and c-AMP. The discovery of c-AMR established one of the fundamental principles of almost all vital processes. And the culmination of research on carbohydrate metabolism was the unrivaled works of Peter Mitchell, who studied the course of biochemical reactions in cells relative to certain intracellular “landmarks” and who formulated the chemiosmotic theory of oxidative phosphorylation that underlies bioenergetics
Доп.точки доступа:
Данилова, В. М.
Комісаренко, С. В.
Гарден, Артур (вчений-хімік, професор ; 1865-1940) \о нем\
Ейлер-Гельпін, Ганс Карл Август Симон (вчений-хімік, доктор наук, професор ; 1873-1964) \о нем\
Радниц, Корі ОГерті Тереза (вчений, лікар-педіатр, психіатр, доктор наук ; 1896-1957) \о ней\
Корі, Карл Фердинанд (вчений-зоолог, професор ; 1896-1984) \о нем\
Усай, Бернардо Альберто (вчений-фізіолог ; 1887-1971) \о нем\
Сазерленд, Е. (лікар-хірург, фармаколог, професор фізіоллогії ; 1915-1974) \о нем\
Лелуар, Луїс Федеріко (лікар-фізіолог, біохімік ; 1906-1987) \о нем\
Кребс, Ганс (лікар-клініцист, приват-доцент ; 1900-1981) \о нем\
Ліпман, Фріц (фармаколог-біохімік, професор ; 1899-1986) \о нем\
Мітчелл, Пітер (біохімік, д-р наук ; 1920-1992) \о нем\

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