Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vari S. G.
Заглавие : Inflammation is the common mechanism of diseases (CMD) in COVID-19 disease during pregnancy and in gestational diabetes mellitus
Место публикации : The Ukrainian biochemical journal. - Київ, 2021. - Vol. 93, № 2. - С. 5 (Шифр УУ60/2021/93/2)
MeSH-главная: БЕРЕМЕННОСТИ ОСЛОЖНЕНИЯ -- PREGNANCY COMPLICATIONS
ДИАБЕТ ГЕСТАЦИОННЫЙ -- DIABETES, GESTATIONAL
КОРОНАВИРУСНЫЕ ИНФЕКЦИИ -- CORONAVIRUS INFECTIONS
КОМОРБИДНОСТЬ -- COMORBIDITY
Аннотация: The Regional Cooperation for Health, Science and Technology (RECOOP HST) Consortium, led by Cedars-Sinai Medical Center was formed in 2006, was transformed into an Association in 2012 and includes 17 universities and academic organizations from eight countries: seven in Central and Eastern Europe (Croatia, Czech Republic, Hungary, Poland, Romania, Slovakia, Ukraine) and the United States. RECOOP builds multinational, multidisciplinary collaborations, and assists as well as coordinates the research activities of the sixteen research groups that are the Cedars-Sinai Medical Center – RECOOP Research Centers (CRRCs). https://www.cedars-sinai.org/research/administration/recoop.html.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kobylinska L., Khylyuk D., Subtelna I., Kitsera M., Lesyk R.
Заглавие : In silico identification and biochemical validation of plausible molecular targets of 4-thiazolidinone derivative les-3833 as a potential anticancer agent
Место публикации : The Ukrainian biochemical journal. - Київ, 2021. - Vol. 93, № 2. - С. 7-23 (Шифр УУ60/2021/93/2)
MeSH-главная: ПРОТИВООПУХОЛЕВЫЕ СРЕДСТВА -- ANTINEOPLASTIC AGENTS
ТИАЗОЛИДИНЫ -- THIAZOLIDINES
Аннотация: Synthetic 4-thiazolidinone derivatives have a broad range of pharmacologic activities. Thus, 4-thiazolidinones are being investigated to create new molecules and develop active pharmaceutical substances for anticancer treatment. In our previous study, we investigated the pyrazoline-thiazolidinone-isatin conjugates, and determined that Les-3833 was the most active compound and might act through inhibition of PARP-, MAPK-, JNK-, Bcl-2-, CDK1/cyclin B, and/or the caspase family. The aim of this research was to perform molecular docking studies to enable the construction of a pharmacophore model for the Les-3833 compound and investigate probable biological targets. Pharmacophore modeling software packages performed molecular docking studies of probable biological targets and enabled the construction of a pharmacophore model. Docking models of Les-3833 with 11 enzymes involved in apoptotic mechanisms were studied. Based on the pharmacophore modeling results for all 11 enzymes, Les-3833 is predicted to be most active in Chk‑1, caspase-6, and caspase-8. Immunoblot analysis proved that the application of Les-3833 led to inhibition of Ser345 phosphorylation, which is induced by etoposide, the most important modification responsible for Chk‑1 activity. Taken together with the results of the docking studies, several mechanisms for the expression of antitumor activity by 4-thiazolidinones are suggested, and such multi-affinity is a characteristic feature of all these derivatives. The docking analysis confirmed the affinity of test compound Les-3833 for a topoisomerase II inhibitor and a high possibility of inhibitory interaction with Chk-1, caspase-6, and caspase-8
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shevchuk O. O., Panasiuk Ya. V., Korda M. M.
Заглавие : Locally delivered lovastatin-containing chitosan nanoparticles promote bone regeneration in rats
Место публикации : The Ukrainian biochemical journal. - Київ, 2021. - Vol. 93, № 2. - С. 24-38 (Шифр УУ60/2021/93/2)
MeSH-главная: ХИТОЗАН -- CHITOSAN
НАНОЧАСТИЦЫ -- NANOPARTICLES
КОСТНАЯ РЕГЕНЕРАЦИЯ -- BONE REGENERATION
ЭУКАРИОТЫ -- EUKARYOTA
Аннотация: Hypolipidemic statins can stimulate osteoregeneration. However, such effects are observed only after administration methods that are unacceptable for patients (prolonged infusions or huge oral doses). The aim of our research was to compare the osteoregeneration effects of lovastatin administered alone as a common pharmaceutical formulation and as lovastatin-containing chitosan nanoparticles (LCCN) in a drill-hole model of bone damage in rats. White inbred rats were randomly divided into four groups: group 1 – intact rats; group 2 – rats with bone defect without treatment (control group); group 3 – rats with bone defect, which received common pharmaceutical formulation of lovastatin at doses of 0.1, 1.0 and 5.0 mg/kg; group 4 – rats, which received 0.1 mg/kg lovastatin in the form of lovastatin-containing chitosan nanoparticles (LCCN). A dental drill of 2.0 mm in diameter was used to form the tibial bone defect. Rats were sacrificed 3, 7, 14 and 28 days after bone defect formation. Calcium (Ca), phosphorus (P) and sialic acid concentrations, alkaline and acidic phosphatase activities, mineralization index, and collagenolytic activity were measured in blood serum. Computed tomography (CT) and histological study were used to estimate the regenerative processes in the bone. It was found that therapeutic doses of lovastatin (0.1 and 1.0 mg/kg) are ineffective for bone defect healing. Only high doses of lovastatin (5.0 mg/kg) promote osteoregeneration. LCCN were more efficient compared to lovastatin alone, as confirmed by CT examination of bone defects and significant changes of Ca, P, and sialic acid concentrations, alkaline and acidic phosphatase activities, mineralization index, and collagenolytic activity. Lovastatin-containing chitosan nanoparticles effectively enhance fracture healing in used preclinical model. This finding suggests the possibility that a similar approach may be effective in hastening fracture repair in humans
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Finiuk N. S., Klyuchivska O. Yu., Ivasechko I. I., Mitina N. E., Ostapiuk Yu. V., Obushak M. D., Zaichenko O. S., Babsky A. M., Stoika R. S.
Заглавие : Effect of a novel thiazole derivative and its complex with a polymeric carrier on stability of DNA in human breast cancer cells
Место публикации : The Ukrainian biochemical journal. - Київ, 2021. - Vol. 93, № 2. - С. 39-52 (Шифр УУ60/2021/93/2)
MeSH-главная: МОЛОЧНОЙ ЖЕЛЕЗЫ НОВООБРАЗОВАНИЯ -- BREAST NEOPLASMS
ТИАЗОЛЫ -- THIAZOLES
Аннотация: Thiazole derivatives are perspective antitumor compounds characterized by a broad range of bioactivity, while polymeric carriers are widely used to enhance the efficiency of biological action of drugs, improve their biocompatibility and water solubility. Previously, we identified that the thiazole-based derivative BF1 (N-(5-benzyl-1,3-thiazol-2-yl)-3,5-dimethyl-1-benzofuran-2-carboxamide) possessed differential toxicity towards targeted tumor cell lines. The aim of the present work was to investigate the action in vitro of BF1 and its complex with the polymeric carrier (PC) poly(PEGMA-co-DMM) (BF1-РС complex) towards human breast adenocarcinoma cells of the MDA-MB-231 and MCF-7 lines. DNA comet analysis, diphenylamine DNA fragmentation assay, gel retardation assay of plasmid DNA, DNA intercalation assay using methyl green dye and fluorescent microscopy were used to study the effects of BF1 on DNA stability in breast cancer cells. The ІС50 of cytotoxic action towards MDA-MB-231 cells was 26.5 ± 2.9 µМ for BF1, while the ІС50 for the BF1-PC complex was 6.9 ± 0.4 µМ, and the PC demonstrated low toxicity (ІС50 ˃ 50 µМ). The BF1-PC complex possessed higher toxicity towards MCF-7 cells than free BF1, with ІС50 of 9.6 ± 0.8 µМ and 15.8 ± 0.9 µМ, respectively. BF1 and BF1-PC induced an increase in the number of damaged cells of the MDA-MB-231 line with blebbing of plasma membrane, condensed chromatin and/or fragmented nucleus and micronuclei formation. Both BF1 and the BF1-PC complex induced single-strand breaks in DNA and its fragmentation in treated MDA-MB-231 cells. The studied compounds were not bound to plasmid DNA and did not intercalate into DNA molecules
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Poshyvak O. B., Pinyazhko O. R., Godlevsky L. S.
Заглавие : Oxidative stress suppression contributes to antiseizure action of axitinib and rapamycin in pentylenetetrazol-induced kindling
Место публикации : The Ukrainian biochemical journal. - Київ, 2021. - Vol. 93, № 2. - С. 53-60 (Шифр УУ60/2021/93/2)
MeSH-главная: ПРОТИВОСУДОРОЖНЫЕ СРЕДСТВА -- ANTICONVULSANTS
СИРОЛИМУС -- SIROLIMUS
КОРАЗОЛ -- PENTYLENETETRAZOLE
ОКСИДАТИВНЫЙ СТРЕСС -- OXIDATIVE STRESS
Аннотация: Rapamycin and axitinib block different kinases in signaling pathways such as PI3K-Akt-mTOR and BDNF-TrkB, respectively. Both have antiseizure and antioxidative actions, which justify studying the combined effects of these drugs upon seizures and oxidative stress in the chronic model of epilepsy. The investigation aimed to look for the combined effect of rapamycin and axitinib upon pentylenetetrazol (PTZ)-kindled seizures and oxidative stress. Experiments were performed on 300 two- to four-month-old Wistar male rats, which had been kindled daily with PTZ (35.0 mg/kg, i.p.). Malondialdehyde (MDA) level, superoxide dismutase (SOD) activity, and glutathione (GSH) level were determined in brain tissues of kindled rats before and after the treatment. The analysis of antiseizure and antioxidative actions was performed using ED50 of rapamycin and axitinib, with their combined administration using graded dosages of ED50 of each drug. The median effective dose (ED50) for rapamycin and axitinib was 0.93 and 4.97 mg/kg, respectively. ED50 of rapamycin when combined with axitinib (2.0 mg/kg) was 0.60 mg/kg, which was reduced by 35.6% when compared with the ED50 administered alone (P 0.05). The MDA level increased from 152.9±24.8 to 388.3±49.2 nmol/mg of protein (P 0.05), while SOD activity reduced from 11.14±2.33 to 3.54±1.08 IU/mg of protein (P 0.05) in brain tissues of the kindled rats. Combined treatment with rapamycin (0.56 mg/kg, i.p.) and axitinib (2.0 mg/kg, i.p.) resulted in a significant rise in SOD activity (11.09±1.86 IU/mg) and GSH level (7.32±1.34 µg/mg) when compared with the kindled rats (P 0.05). Combined axitinib and rapamycin therapy have an antiepileptic and antioxidative effect on PTZ-kindled seizures
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Korolova D. S., Stohnii Y. M., Gryshchuk V. I., Zhuk S. I., Us I. V., Chernyshenko T. M., Kostiuchenko O. P., Klymenko K. P., Platonov O. M., Ivashchenko O. I., Chernyshenko V. O.
Заглавие : Thromboelastographic study of fibrin clot and molecular basis of maximum clot firmness
Место публикации : The Ukrainian biochemical journal. - Київ, 2021. - Vol. 93, № 2. - С. 62-70 (Шифр УУ60/2021/93/2)
MeSH-главная: ФИБРИН -- FIBRIN
ТРОМБОЭЛАСТОГРАФИЯ -- THROMBELASTOGRAPHY
Аннотация: Maximum clot firmness (MCF) is the main parameter of thromboelastography (TEG) reflecting the stability of a clot. In this work, we looked for markers that can influence the enhancement of MCF and detected molecular markers and blood clotting parameters that can be involved in such mechanisms. Blood samples of pregnant women with placental disorders were collected in the Kyiv Perinatal Center. TEG was performed on whole blood in EXTEM and INTEM tests. APTT, INR, fibrinogen concentration and platelet aggregation were measured using traditional laboratory approaches. D-dimer was detected in sandwich ELISA using monoclonal antibodies III-3B and II-4D. The relative cross-linking activity of factor XIIIa was measured by the direct quantification of the cross-linked γ-chain of fibrin using Western-Blotting with monoclonal antibody II-4D. D-dimer and fibrinogen concentrations, clotting time in the APTT test, INR and rate of platelet aggregation did not correlate with the MCF. However, we found positive correlations of MCF with factor XIIIa activity: 0.51 and 0.87 for EXTEM and INTEM, respectively. These data indicate that for normal and slightly increased fibrinogen concentrations, fibrin clot firmness will depend mostly on the activity of factor XIIIa. Thus the direct determination of factor XIIIa activity in blood plasma of patients can be relevant for predicting the risk of intravascular coagulation. Evaluation of the content and activity of individual clotting factors or other components of the coagulation system can be useful additions to the TEG diagnostics and should not be neglected
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pavlyshyn H. A., Shulhai A.-M. A.
Заглавие : The association between vitamin D status and cardiometabolic disorders in overweight and obese adolescents
Место публикации : The Ukrainian biochemical journal. - Київ, 2021. - Vol. 93, № 2. - С. 72-81 (Шифр УУ60/2021/93/2)
MeSH-главная: ОЖИРЕНИЕ -- OBESITY
СЕРДЕЧНО-СОСУДИСТЫЕ БОЛЕЗНИ -- CARDIOVASCULAR DISEASES
ВИТАМИН D -- VITAMIN D
Аннотация: An increase in the prevalence of overweight and obese adolescents is often combined with a low level of vitamin D that may be associated with lipid and carbohydrate metabolism disorders, which underlie the development of arterial hypertension and cardiometabolic changes. This work aimed to determine the association between vitamin D status and the main parameters of lipid and carbohydrate metabolism, and anthropometric measurements. A total of 196 adolescents (129 boys and 67 girls) from the Ternopil region were examined. Their mean age was 15.5 ± 2.3 years. Based on the body mass index (BMI): 60 adolescents had normal body weight, 60 were overweight, and 76 were obese. Anthropometric measurements and general examinations were performed. In blood serum 25(OH)D levels and the main parameters of lipid and carbohydrate metabolism were determined. Low levels of 25(OH)D were found. Prevalence of vitamin D deficiency among adolescents with BMI up to the 85th percentile was 56.7%, with BMI in the 85-97th percentiles was 70.0% and with BMI over the 97th percentile was 77.6%. Serum 25(OH)D levels in overweight and obese adolescents had a significant correlation with BMI (r = -0.427, P 0.001), waist circumference (r
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pavlyshyn H. A., Kozak K. V., Hlushko K. T.
Заглавие : Investigating the connections between night eating syndrome and metabolic syndrome in children
Место публикации : The Ukrainian biochemical journal. - Київ, 2021. - Vol. 93, № 2. - С. 83-94 (Шифр УУ60/2021/93/2)
MeSH-главная: ПИЩИ ПРИЕМА НАРУШЕНИЯ -- EATING DISORDERS
МЕТАБОЛИЧЕСКИЙ СИНДРОМ X -- METABOLIC SYNDROME X
ДЕТИ -- CHILD
Аннотация: Eating disorders are considered to be the cause of obesity, particularly its abdominal type and metabolic syndrome (MS). Until recently, night eating syndrome (NES) and MS were documented only in the adult population, but nowadays they are also seen among children. Therefore, the aim of our study was to determine the association between NES and MS. A total of 120 overweight (18.33%) and obese (81.67%) children 10-17 years of age were examined (27 girls (22.5%) and 93 boys (77.5%)). Anthropometric and blood pressure measurements were performed for all children. Serum triglycerides, high-density lipoproteins and glucose levels were assessed. Abdominal obesity was found in 70% of cases. NES was diagnosed in 20.83% of children. MS was found in 34.17% of participants. MS was more prevalent in the NES group (56.00% (95% CI 30.62; 93.96)), compared with the non-NES group (28.42% (95% CI 18.73; 41.35)) (P 0.05). NES increases the risk of MS development by 3 times (OR 3.21 (95% CI 1.29‒7.94); P
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Hlushko K. T., Pavlyshyn H. A., Kozak K. V.
Заглавие : Clinical and immunological peculiarities of parasitic infections in children with digestive system disorders in Western Ukraine
Место публикации : The Ukrainian biochemical journal. - Київ, 2021. - Vol. 93, № 2. - С. 96-105 (Шифр УУ60/2021/93/2)
MeSH-главная: ПИЩЕВАРИТЕЛЬНОЙ СИСТЕМЫ БОЛЕЗНИ -- DIGESTIVE SYSTEM DISEASES
ПАРАЗИТАРНЫЕ БОЛЕЗНИ -- PARASITIC DISEASES
ДЕТИ -- CHILD
Аннотация: Diseases of the digestive system such as gastroduodenal disorders (GDD) and hepatobiliary diseases (HBD) are fairly common among children. At the same time, intestinal parasites are widespread gastrointestinal tract infectious agents. We examined 108 children with GDD (n = 54) and HBD (n = 54) (mean age 11.8 ± 4.3 years) who were treated in a children’s hospital. The following were performed: blood test for specific Ig to Ascaris lumbricoides, Toxocara canis; feces analysis for helminth eggs, Giardia cysts; and pinworm test. The serum level of interleukin-4 (IL-4) was measured in 97 of 108 children. Overall parasitic co-infections were found in 60.2% of children: giardiasis in 30.6%, toxocariasis in 17.6%, ascariasis seropositivity in 13.9% and enterobiasis in 8.3% of cases. Parasitic infections (PIs) occurred in 72.2% of the HBD group and in 48.2% of the GDD group (P = 0.01). Among the PIs only ascariasis was found more often in the HBD group (22.2%) compared with the GDD group (5.6%) (P = 0.01). The mean age of children who were positive for PI (PI+) was 9.9 ± 4.6 years; while in the PI negative (PI–) group the mean age was 12.8 ± 3.3 years (P 0.001). In GDD the IL-4 level in PI+ group was 9.3 ± 0.9 pg/ml in the versus 6.9 ± 1.8 pg/ml in the PI‒ group (P
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kucher S. V., Lototska O. V.
Заглавие : Inclusion of enterosorbents in anti-inflammatory therapy improve treatment effectiveness in COPD patients during exacerbations
Место публикации : The Ukrainian biochemical journal. - Київ, 2021. - Vol. 93, № 2. - С. 107-114 (Шифр УУ60/2021/93/2)
MeSH-главная: ЛЕГКИХ БОЛЕЗНИ ОБСТРУКТИВНЫЕ -- LUNG DISEASES, OBSTRUCTIVE
ЦИТОКИНЫ -- CYTOKINES
ЭНТЕРОСОРБЦИЯ -- ENTEROSORPTION
Аннотация: Inflammation plays a key role in the development of chronic obstructive pulmonary disease (COPD), and manifests as increased levels of inflammation markers such as cytokines. The purpose of this study was to examine the effect of enterosorbents on IL-1β, TNF-α and IL-10 levels in COPD patients with exacerbations, while taking into account their ages. Patients were divided into two age groups: age group 1 (40-59 years) and age group 2 (60 years). Levels of IL-1β, TNF-α and IL-10 were determined by enzyme-linked immunosorbent assay. Levels of pro-inflammatory and anti-inflammatory cytokines were increased in both age groups of COPD patients during exacerbations compared to the healthy control group. Pro-inflammatory cytokines (IL-1β, TNF-α) increased more in age group 2. Basic therapy (BT) had positive impacts. Addition to BT of the enterosorbents Enterosgel and Carboline decreased serum levels of IL-1β, TNF-α and IL-10 in both age groups more than after BT alone. Greater changes were observed in age group 2. We found that addition of the enterosorbents Carboline or Enterosgel to basic therapy of COPD patients during exacerbations decreased levels of IL-1β, TNF-α and IL-10 in patients of both age groups (40-59 years and 60 years)
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Danylova T. V., Komisarenko S. V.
Заглавие : Double Nobel prize winner: Frederick Sanger - the father of genomics
Место публикации : The Ukrainian biochemical journal. - Київ, 2021. - Vol. 93, № 2. - С. 116-122 (Шифр УУ60/2021/93/2)
MeSH-главная: НОБЕЛЕВСКАЯ ПРЕМИЯ -- NOBEL PRIZE
Аннотация: This paper aims to outline briefly the main stages of Frederick Sanger’s scientific activity – the only person to have won two Nobel Prizes in Chemistry (1958, 1980). His work on the structure of proteins, especially that of insulin, and the determination of base sequences in nucleic acids made an immense impact on the development of biochemistry and especially on the development of a new scientific field – molecular biology. His methods for determining the primary structure of proteins and nucleic acids helped biochemists and molecular biologists to determine the structure of many proteins and nucleic acids and laid the basis for genetic engineering
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