Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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Шифр: УУ60/2017/89/4
Журнал

The Ukrainian biochemical journal [Текст]. - Выходит раз в два месяца
2017г. Т. 89 № 4

Содержание:
Babenko, L. M. Lipoxygenase activity in ontogenesis of ferns Salvinia natans and Polystichum aculeatum / L. M. Babenko, T. D. Skaterna, I. V. Kosakivska. - P.5-12
Chekh, B. O. Characteristics of novel polymer based on pseudo-polyamino acids GluLa-DPG-PEG600: binding of albumin, biocompatibility, biodistribution and potential crossing the blood-brain barrier in rats / B. O. Chekh [et al.]. - P.13-21
Другие авторы: Ferens M. V., Ostapiv D. D., Samaryk V. Y., Varvarenko S. M., Vlizlo V. V.
Palagina, I. A. Pro-/antioxidant reactions and nitrogen oxide metabolism under sub-chronic effect of succinic acid derivatives / I. A. Palagina. - P.22-33
Kolupaev, Yu. E. Induction of plant cells heat resistance by hydrogen sulfide donor is mediated by H2O2 generation with participation of NADPH oxidase and superoxide dismutase / Yu. E. Kolupaev, E. N. Firsova, T. O. Yastreb. - P.34-42
Khomochkin, A. P. Reversible pH-dependent activation/inactivation of CF1-ATPase of spinach chloroplasts / A. P. Khomochkin [et al.]. - P.43-48
Другие авторы: Onoiko O. B., Semenikhin A. V., Zolotareva O. K.
Gozhenko, A. I. The experimental investigation of fibrinolytic system under the influence of flocalin in conditions of acute hypoxic kidney injury / A. I. Gozhenko [et al.]. - P.49-55
Другие авторы: Gubsky Yu. I., Filipets N. D., Filipets O. O., Gozhenko O. A.
Prysiazhniuk, A. I. Role of peripheral dopaminergic system in the pathogenesis of experimental colitis in rats / A. I. Prysiazhniuk [et al.]. - P.56-67
Другие авторы: Rudyk M. P., Chervinska T. M., Dovbynchuk T. V., Opeida I. V., Skivka L. M., Tolstanova G. M.
Horid’ko, T. M. Antistress effects of N-stearoylethanolamine in rats with chronic social stress / T. M. Horid’ko [et al.]. - P.68-76
Другие авторы: Kosiakova H. V., Berdyshev A. G., Meged O. F., Gudz E. A., Onopchenko O. V., Asmolkova V. S., Lozova V. M., Tukalenko E. V., Bondarenko O. V., Tubalzeva I. I., Kovalenko O. A., Makarchuk M. Y., Hula N. M.
Subekti, N. Determination of the major compounds in the extract of the subterranean termite Macrotermes gilvus Hagen digestive tract by GC-MS method / N. Subekti [et al.]. - P.77-82
Другие авторы: Fibriana F., Widyaningrum P., Adfa M.
Виноградова, Р. П. Науково-практичні розробки відділу молекулярної біології Інституту біохімії ім. О. В. Палладіна НАН України / Р. П. Виноградова, М. В. Григор’єва, В. М. Данилова. - P.83-90
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Kolupaev, Yu. E.
Induction of plant cells heat resistance by hydrogen sulfide donor is mediated by H2O2 generation with participation of NADPH oxidase and superoxide dismutase / Yu. E. Kolupaev, E. N. Firsova, T. O. Yastreb // The Ukrainian Biochemical Journal. - 2017. - Том 89, N 4. - P34-42

MeSH-главная:
ПШЕНИЦА -- TRITICUM (действие лекарственных препаратов)
НАТРИЯ СОЕДИНЕНИЯ -- SODIUM COMPOUNDS
Аннотация: The participation of enzymatic systems carrying out generation and conversion of reactive oxygen species (ROS), in realization of the stress-protective effect of hydrogen sulfide (H₂S) on wheat coleoptile cells was investigated. It has been shown that the treatment of isolated coleoptiles with a 100 μM hydrogen sulfide donor sodium hydrosulfide (NaHS) caused a transient enhancement of the generation of superoxide anion radical (O₂^•–), an increase of hydrogen peroxide content and superoxide dismutase (SOD) activity in them. The increase in ROS generation was eliminated by the inhibitor of NADPH oxidase imidazole, but not by the peroxidase inhibitor sodium azide. Treatment of coleoptiles with SOD inhibitor sodium diethyldithiocarbamate (DDC) enhanced the generation of O₂]^•– and neutralized the effect of increasing H₂O2 content induced by NaHS. One day after treatment with the H₂S donor, the generation of ROS decreased to a control level, while the activity of antioxidant enzymes increased markedly and the resistance of coleoptiles to damaging heating was increased. These effects of the hydrogen sulfide donor were eliminated by coleoptiles’ treatment with inhibitors of NADPH oxidase (imidazole) and SOD (DDC). It was concluded that both NADPH oxidase, generating O₂^•– , and SOD, which turns it into H₂O2 performing signaling functions, are involved in the formation of a signal that induces protective systems and causes an increase in heat resistance of plant cells
Доп.точки доступа:
Firsova, E. N.
Yastreb, T. O.

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Шифр: УУ60/2018/90/5
Журнал

The Ukrainian biochemical journal [Текст]. - Выходит раз в два месяца
2018г. Т. 90 № 5

Содержание:
Pushkarev, V. M. Biochemical aspects of the combined use of taxanes, irradiation and other antineoplastic agents for the treatment of anaplastic thyroid carcinoma / V. M. Pushkarev [et al.]. - P.5-18
Другие авторы: Kovzun O. I., Pushkarev V. V., Guda B. B., Tronko M. D.
Borzova, N. V. Influence of chemical reagents and UV irradiation on the activity of Penicillium canescens α-galactosidase / N. V. Borzova, L. D. Varbanets. - P.19-27
Osadchuk, T. V. Influence of organic solvents on the furin activity / T. V. Osadchuk [et al]. - P.28-33
Другие авторы: Shybyryn O. V., Semyroz A. V., Kibirev V. K.
Veklich, T. O. Calix[4]arene C-956 selectively inhibits plasma membrane Ca2+,Mg2+-ATPase in myometrial cells / T. O. Veklich [et al.]. - P.34-42
Другие авторы: Skrabak O. A., Nikonishyna Yu. V., Rodik R. V., Kalchenko V. I., Kosterin S. O.
Afanasieva, K. S. DNA loops after cell lysis resemble chromatin loops in an intact nucleus / K. S. Afanasieva, V. V. Olefirenko, A. V. Sivolob. - P.43-49
Yastreb, T. O. Action of methyl jasmonate and salt stress on antioxidant system of Arabidopsis plants defective in jasmonate signaling genes / T. O. Yastreb [et al.]. - P.50-59
Другие авторы: Kolupaev Yu. E., Shvidenko N. V., Dmitriev A. P.
Storozhuk, B. G. Overall hemostasis potential of the blood plasma and its relation to some molecular markers of the hemostasis system in patients with chronic renal disease of stage VD / B. G. Storozhuk [et al.]. - P.60-70
Другие авторы: Pyrogova L. V., Chernyshenko T. M., Kostiuchenko O. P., Kolesnikova I. M., Platonova T. M., Storozhuk O. B., Storozhuk L. O., Bereznitsky G. K., Tsap P. Yu., Masenko O. O., Makogonenko E. M., Lugovskoy E. V.
Falfushynska, H. I. Evaluation of metallothioneins, oxidative stress and signs of cytotoxicity in young obese women / H. I. Falfushynska [et al.]. - P.71-80
Другие авторы: Horyn O. I., Khoma V. V., Tereshchuk G. V., Osadchuk D. V., Rusnak N. I., Stoliar O. B.
Dziuba, O. S. The effect of N-stearoylethanolamine on adipocytes free cholesterol content and phospholipid composition in rats with obesity-induced insulin resistance / O. S. Dziuba [et al.]. - P.81-90
Другие авторы: Hudz Ie. A., Kosiakova H. V., Horid’ko T. M., Klimashevsky V. M., Hula N. M.
Krynytska, I. Ya. The indices of nitrogen (II) oxide system in experimental hepatopulmonary syndrome / I. Ya. Krynytska, M. I. Marushchak. - P.91-97
Gudkova, O. O. Purification procedure and assay for the activity of lysyl oxidase / O. O. Gudkova [et al.]. - P.98-105
Другие авторы: Latyshko N. V., Zaitseva O. V., Shandrenko S. G.
Kosterin, S. O. Founder of molecular immunology in Ukraine, well-known political and public figure. On the 75th birthday of Academician of the NAS of Ukraine S. V. Komisarenko / S. O. Kosterin, V. M. Danilova. - P.106-116
Matyshevska, O. P. The FEBS3+ Meeting - XIth Parnas Conference - Young Scientists Forum ’Biochemistry and Molecular Biology for Innovative Medicine’ : 3-5 september 2018, Kyiv, Ukraine / O. P. Matyshevska. - P.117-119
The Nobel Prize in Physiology or Medicine 2018. - P.120
The Nobel Prize in Chemistry 2018. - P.121
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Action of methyl jasmonate and salt stress on antioxidant system of Arabidopsis plants defective in jasmonate signaling genes [Text] / T. O. Yastreb [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 5. - P50-59

MeSH-главная:
КРЕСТОЦВЕТНЫЕ -- BRASSICACEAE (генетика, действие лекарственных препаратов)
Аннотация: Role of jasmonate signaling in the regulation of stress-protective systems in Arabidopsis under salt stress remains insufficiently studied. For its clarification, comparative studies with mutants lacking various protein components of jasmonate signaling are advisable. In this connection, effects of methyl jasmonate (MJ, 50 μM) and salt stress (NaCl, 150 mM) on functioning of antioxidant and osmoprotective systems of wild-type Arabidopsis plants (Col-0) and ones defective in jasmonate signaling, namely coi1 (mutant for gene coding the protein COI1, which participates in removal of repressor proteins of transcription factors of jasmonate signaling) and jin1 (mutant defective in gene encoding the transcription factor JIN1/MYC2, one of the key in jasmonate signaling), were investigated. Salt stress inhibited growth of plants of all three genotypes. Treatment with MJ before salt stress positively influenced only the growth of wild-type plants. In contrast to mutants coi1 and jin1, Col-0 plants treated with MJ, under conditions of salt stress, kept close to the control values of water and total chlorophylls content, and the content of carotenoids increased. The coi1 plants under normal conditions differed from wild-type plants and jin1 mutants by reduced activity of guaiacol peroxidase and catalase and increased proline content. Treatment with MJ did not affect the activity of antioxidant enzymes and proline content in both mutants defective in jasmonate signaling. Under salt stress, the activity of superoxide dismutase, catalase and guaiacol peroxidase, as well as the content of proline and anthocyanins, in wild-type plants treated with MJ, were significantly higher than in control plants. The role of jasmonate-dependent protective systems in resistance of Arabidopsis plants to salt stress is discussed
Доп.точки доступа:
Yastreb, T. O.
Kolupaev, Yu. E.
Shvidenko, N. V.
Dmitriev, A. P.

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Шифр: УУ60/2018/90/6
Журнал

The Ukrainian biochemical journal [Текст]. - Выходит раз в два месяца
2018г. Т. 90 № 6

Содержание:
Asmaa, Missoum. The role of gene GJB2 and connexin 26 in hearing impairment / Missoum Asmaa. - P.5-11
Kolupaev, Yu. E. Phenylalanine ammonia-lyase activity and content of flavonoid compounds in wheat seedlings at the action of hypothermia and hydrogen sulfide donor / Yu. E. Kolupaev [et al.]. - P.12-20
Другие авторы: Horielova E. I., Yastreb T. O., Popov Yu. V., Ryabchun N. I.
Chernyshenko, V. O. Haemostasis modulation by calix[4]arene methylenebisphosphonic acid C-145 and its sulfur-containing analogue / V. O. Chernyshenko [et al.]. - P.21-30
Другие авторы: Savchuk O. V., Cherenok S. O., Silenko O. M., Negelia A. O., Kasatkina L. O., Pirogova L. V., Didkivskyi V. A., Yusova O. I., Kalchenko V. I., Garmanchuk L. V., Grinenko T. V., Lugovskoy E. V., Komisarenko S. V.
Tykhomyrov, A. A. Plasminogen modulates formation of reactive oxygen species in human platelets / A. A. Tykhomyrov [et al.]. - P.31-40
Другие авторы: Zhernosekov D. D., Guzyk M. M., Korsa V. V., Grinenko T. V.
Siromolot, A. A. Specificity and sensitivity of the new test for serological evaluation of tuberculosis using MPT83-MPT63 fusion antigen / A. A. Siromolot [et al.]. - P.41-48
Другие авторы: Chudina T. O., Danilova I. S., Rekalova O. M., Kolybo D. V., Komisarenko S. V.
Lylyk, M. P. Effects of alpha-ketoglutarate on lifespan and functional aging. Of Drosophila melanogaster flies / M. P. Lylyk [et al.]. - P.49-61
Другие авторы: Bayliak M. M., Shmihel H. V., Storey J. M., Storey K. B., Lushchak V. I.
Purkan, P. Immunogenicity assay of KatG protein from Mycobacterium tuberculosis in mice: preliminary screening of TB vaccine / P. Purkan [et al.]. - P.62-69
Другие авторы: Budiyanto R., Akbar R., Wahyuningsih S. P., Retnowati W.
Zavodovskyi, D. O. Influence of C60 fullerene on the ischemia-reperfusion injury in the skeletal muscle of rat limb: mechanokinetic and biochemical analysis / D. O. Zavodovskyi [et al.]. - P.70-81
Другие авторы: Zay S. Yu., Matvienko T. Yu., Prylutskyy Yu. I., Nurishchenko N. Y., Paradizova S. S., Bezuh L. L., Ritter U., Scharff P.
Nakonechna, O. A. Disturbance of the transmembrane phosphatidylserine asymmetry in hepatocytes as an apoptosis marker under the action of xenobiotics on rats / O. A. Nakonechna, L. A. Babijchuk, A. I. Bezrodna. - P.82-88
Aminizadeh, S. The effects of PDK4 inhibition on AMPK protein levels and PGC-1α gene expression following endurance training in skeletal muscle of Wistar rats / S. Aminizadeh, Y. Masoumi-Ardakani, B. Shahouzehi. - P.89-96
Horid’ko, T. M. Preventive effect of N-stearoylethanolamine on memory disorders, blood and brain biochemical parameters in rats with experimental scopolamine-induced cognitive impairment / T. M. Horid’ko [et al.]. - P.97-109
Другие авторы: Kosiakova H. V., Berdyshev A. G., Meged O. F., Onopchenko O. V., Klimashevsky V. M., Tkachenko O. S., Bazylianska V. R., Kholin V. O., Peschana K. O., Mykhalskiy S. A., Hula N. M.
Hurmach, Y. The effect of intranasally administered TLR3 agonist larifan on metabolic profile of microglial cells in rat with C6 glioma / Y. Hurmach [et al.]. - P.110-119
Другие авторы: Rudyk M., Svyatetska V., Senchylo N., Skachkova O., Pjanova D., Vaivode K., Skivka L.
Marounek, M. Long-term hypocholesterolemic effect of amidated alginate in rats / M. Marounek [et al.]. - P.120-125
Другие авторы: Volek Z., Taubner T., Duskova D., Kalachniuk L.
Данилова, В. М. Внесок Нобелівських лауреатів початку ХХ ст. в розвиток молекулярної імунології: Е. Берінг, І. І. Мечников, П. Ерліх, Ш. Ріше, Ж. Борде, К. Ландштейнер / В. М. Данилова, Р. П. Виноградова, С. В. Комісаренко. - P.126-142
Дмитро Олексійович Мельничук. До 75-річчя з дня народження. - P.143-144
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Phenylalanine ammonia-lyase activity and content of flavonoid compounds in wheat seedlings at the action of hypothermia and hydrogen sulfide donor [Text] / Yu. E. Kolupaev [et al.] // The Ukrainian Biochemical Journal. - 2018. - Том 90, N 6. - P12-20

MeSH-главная:
ИММУНИТЕТ РАСТЕНИЙ -- PLANT IMMUNITY
ГИПОТЕРМИЯ -- HYPOTHERMIA
СЕРОВОДОРОД -- HYDROGEN SULFIDE
ПШЕНИЦА -- TRITICUM (иммунология, метаболизм)
Аннотация: At present hydrogen sulfide (H2S) is considered as one of the signal mediators in plant cells. However, its role in formation of plant resistance to low temperatures and, in particular, in regulation of secondary metabolism under stress conditions remains poorly understood. The influence of H2S donor sodium hydrosulfide (NaHS) on phenylalanine ammonia-lyase (PAL) activity and content of flavonoids in wheat seedlings at normal temperature (21 °C) and under cold hardening conditions (7 days at 3 °C) was studied. After 2 days of the hardening temperature, a transient increase in PAL activity was noted. Also, activity of the enzyme was increased by treatment of plants with 0.1 or 0.5 mM NaHS under normal temperature conditions and especially at the background of cold hardening. By themselves, the cold hardening and the action of H2S donor caused an increase in total content of flavonoids and amount of anthocyanins. With the combination of hypothermia and treatment of seedlings with NaHS, this effect enlarged and the total content of flavonoids increased by 3.8, and anthocyanins increased by 1.8 times in comparison to the control. Treatment with the H2S donor caused a decrease in content of the lipid peroxidation product malonic dialdehyde in seedlings after the action of hardening temperature, and especially after their freezing at –5 °C. Also, under the influence of NaHS, survival of hardened and unhardened seedlings after cryostress increased. It was concluded that one of the mechanisms of the positive influence of the H2S donor on resistance of wheat seedlings to hypothermia is the PAL-dependent accumulation of flavonoid compounds, which have a high antioxidant activity, and a decrease in effects of secondary oxidative stress
Доп.точки доступа:
Kolupaev, Yu. E.
Horielova, E. I.
Yastreb, T. O.
Popov, Yu. V.
Ryabchun, N. I.

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Шифр: УУ60/2019/91/6
Журнал

The Ukrainian biochemical journal [Текст]. - Выходит раз в два месяца
2019г. Т. 91 № 6 . - 138.92, р.

Содержание:
Mazur, H. M. Dependence of the mitochondrial adaptive capacity of hepatocytes on the oxidative substrates availability / H. M. Mazur [та ін.]. - P.5-14. - Bibliogr. at the end of the art.
Другие авторы: Merlavsky V. M., Manko B. O., Manko V. V.
Mankovska, O. Analysis of Aurora kinases genes expression points on their distinct roles in prostate cancer development / O. Mankovska [та ін.]. - P.15-26. - Bibliogr. at the end of the art.
Другие авторы: Gerashchenko G., Rozenberg E., Stakhovsky E., Kononenko O., Bondarenko Yu., Kashuba V.
Pyrogova, L. V. Comparative analysis of the influence of chlorine and fluorine anions on the fibrin polymerization / L. V. Pyrogova [та ін.]. - P.27-37. - Bibliogr. at the end of the art.
Другие авторы: Bereznitsky G. K., Gogolinskaya G. K., Platonova T. M., Kolesnikova I. M., Masenko O. O., Marunich R. Yu., Tsap P. Yu., Ushenin Yu. V., Makogonenko Y. M., Lugovskoi E. V.
Grinenko, T. Fibrinolysis regulation by platelets retaining plasminogen and tissue-type plasminogen activator on their surface / T. Grinenko [та ін.]. - P.38-48. - Bibliogr. at the end of the art.
Другие авторы: Yusova O., Revka O., Patalakh I., Yatsenko T.
Korotkyi, O. Effect of probiotic composition on oxidative/antioxidant balance in blood of rats under experimental osteoarthritis / O. Korotkyi [та ін.]. - P.49-58. - Bibliogr. at the end of the art.
Другие авторы: Dvorshchenko K., Vovk A., Dranitsina A., Tymoshenko M., Kot L., Ostapchenko L.
Masoumi-Ardakani, Y. Carnitine effects on serum and pancreas inflammatory response in diabetic rats / Y. Masoumi-Ardakani, H. Fallah, B. Shahouzehi. - P.59-66. - Bibliogr. at the end of the art.
Falfushynska, H. I. Oxidative stress and thiols depletion impair tibia fracture healing in young men with type 2 diabetes / H. I. Falfushynska [та ін.]. - P.67-78. - Bibliogr. at the end of the art.
Другие авторы: Horyn O. I., Poznansky D. V., Osadchuk D. V., Savchyn T. О., Krytskyi T. І., Merva L. S., Hrabra S. Z.
Hrabovska, O. I. The concentration of ceruloplasmin in blood of tumor-bearing rats after administration of a dirhenium(III) compound and cisplatin / O. I. Hrabovska [та ін.]. - P.79-85. - Bibliogr. at the end of the art.
Другие авторы: Shamelashvili K. L., Shtemenko O. V., Shtemenko N. I.
Shafaee, M. M.A. Effect of selenium and nano-selenium on cisplatin-induced nephrotoxicity in albino rats / M. M.A. Shafaee [та ін.]. - P.86-95. - Bibliogr. at the end of the art.
Другие авторы: Mohamed H. S., Ahmed S. A., Kandeil M. A.
Gudzenko, T. V. Fatty acids composition of Bacillus subtilis ONU551 lipids / T. V. Gudzenko [та ін.]. - P.96-102. - Bibliogr. at the end of the art.
Другие авторы: Voliuvach O. V., Gorshkova O. G., Ostapchuk А. М., Ivanytsia V. O.
Kolupaev, Yu. E. Hydrogen peroxide as a signal mediator at inducing heat resistance in wheat seedlings by putrescine / Yu. E. Kolupaev [та ін.]. - P.103-111. - Bibliogr. at the end of the art.
Другие авторы: Kokorev A. I., Yastreb T. O., Horielova E. I.
Vovkun, T. V. Corvitin modulates the content of lipids in rat liver bile / T. V. Vovkun [та ін.]. - P.112-121. - Bibliogr. at the end of the art.
Другие авторы: Yanchuk P. I., Shtanova L. Ya., Veselsky S. P., Filimonova N. B., Komarov I. V.
Andrushchenko, T. A. Allelic polymorphisms of DNA repairing genes as markers of resistance to asbestos-containing aerosols / T. A. Andrushchenko [та ін.]. - P.122-128. - Bibliogr. at the end of the art.
Другие авторы: Goncharov S. V., Dosenko V. E., Stroy D. O., Ishchejkin K. E.
Matyshevska, O. P. The contribution of the Nobel laureates to the study of lipid metabolism and its regulation. F. Lynen, K. Bloch, S. Bergström, B. Samuelsson, J. Vane, M. Brown, J. Goldstein / O. P. Matyshevska, V. M. Danilova, S. V. Komisarenko. - P.129-143. - Bibliogr. at the end of the art.
Kosterin, S. O. XIІ Ukrainian Biochemical Congress (30 September – 4 October 2019, Ternopil / S. O. Kosterin [та ін.]. - P.144-149. - Bibliogr. at the end of the art.
Другие авторы: Matyshevska O. P., Danilova V. M., Grigorieva M. V.
The Nobel Prize in Physiology or Medicine 2019. - P.150
The Nobel Prize in Chemistry 2019. - P.151
In memory of O. S. Mikosha. - P.152
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Hydrogen peroxide as a signal mediator at inducing heat resistance in wheat seedlings by putrescine [Текст] = Гідроген пероксид як сигнальний посередник за індукування теплостійкості проростків пшениці путресцином / Yu. E. Kolupaev [та ін.] // The Ukrainian Biochemical Journal. - 2019. - Т. 91, № 6. - С. 103-111. - Bibliogr. at the end of the art.

Рубрики: Путресцин--фарм

MeSH-главная:
ПОЛИАМИНЫ -- POLYAMINES (анализ)
РАСТЕНИЙ-ЛЕКАРСТВ ВЗАИМОДЕЙСТВИЕ -- HERB-DRUG INTERACTIONS (физиология)
ПШЕНИЦА -- TRITICUM (действие лекарственных препаратов, физиология)
МНОГОФУНКЦИОНАЛЬНЫЕ ФЕРМЕНТЫ -- MULTIFUNCTIONAL ENZYMES (анализ)
ГИПЕРТЕРМИЯ ИСКУССТВЕННАЯ -- HYPERTHERMIA, INDUCED (тенденции)
АГГЛЮТИНИНЫ ПРОРОСТКОВ ПШЕНИЦЫ -- WHEAT GERM AGGLUTININS (анализ)
ТАБЛИЦЫ -- TABLES
Аннотация: Polyamines are multifunctional stress metabolites of plants. However, information on the effect of exogenous polyamines on plant resistance to high temperatures is contradictory, and it remains unclear which signal mediators are involved in the realization of their physiological effects. The possible involvement of hydrogen peroxide as a mediator under the action of exogenous diamine putrescine on the resistance of etiolated wheat seedlings (Triticum aestivum L.) to hyperthermia (10-minute heating at 46°C) and the functioning of antioxidant system was investigated. It was established that the treatment of seedlings with putrescine in 0.25–2.5 mM concentrations caused a significant increase in their heat resistance. In response to the putrescine effect, a transient increase in the H₂O₂ content occurred in the root cells. This effect was eliminated by treatment of seedlings with a diamine oxidase inhibitor aminoguanidine and an NADPH oxidase inhibitor imidazole. These inhibitors, as well as the scavenger of hydrogen peroxide dimethylthiourea (DMTU), mitigated the effects of increased heat resistance of seedlings and increased activity of superoxide dismutase and catalase caused by putrescine. Under the influence of DMTU and imidazole, but not aminoguanidine, the effect of increasing the activity of guaiacol peroxidase in the roots of seedlings treated with putrescine was eliminated. The conclusion was made about the role of hydrogen peroxide and the possible participation of diamine oxidase and NADPH oxidase in its formation during the implementation of the stress-protective effect of putrescine on wheat seedlings
Поліаміни належать до мультифункціональних стресових метаболітів рослин. Однак відомості про вплив екзогенних поліамінів на стійкість рослин до високих температур суперечливі, при цьому залишається неясним, які сигнальні посередники задіяні в реалізації їх фізіологічних ефектів. Досліджували можливу участь гідроген пероксиду як посередника за дії екзогенного діаміну путресцину на стійкість етіольованих проростків пшениці (Triticum aestivum L.) до гіпертермії (10-хвилинного прогрівання при 46 °С) і функціонування антиоксидантної системи. Встановлено, що обробка проростків путресцином в концентраціях 0,25–2,5 мМ спричиняла істотне підвищення їх теплостійкості. У відповідь на дію путресцину в клітинах коренів відбувалося транзиторне збільшення вмісту H₂O₂. Такий ефект усувався обробкою проростків інгібітором діаміноксидази аміногуанідином та інгібітором NADPH-оксидази імідазолом. Ці інгібітори, а також скавенджер гідроген пероксиду диметилтіосечовина (ДМТС), нівелювали спричинювані путресцином ефекти підвищення теплостійкості проростків та збільшення активності супероксиддисмутази і каталази. Під впливом ДМТС та імідазолу, але не аміногуанідину, усувався ефект підвищення активності гваяколпероксидази в коренях проростків, оброблених путресцином. Зроблено висновок про роль гідроген пероксиду та можливу участь діаміноксидази і NADPH-оксидази в його утворенні за реалізації стрес-протекторної дії путресцину на проростки пшениці
Доп.точки доступа:
Kolupaev, Yu. E.
Kokorev, A. I.
Yastreb, T. O.
Horielova, E. I.

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