Шифр: УУ60/2017/89/1 Журнал 2017р. Т. 89 № 1 Babenko, L. M. Lipoxygenases and their metabolites in formation of plant stress tolerance / L. M. Babenko [et al.]. - P.5-21 Інші автори: Shcherbatiuk M. M., Skaterna T. D., Kosakivska I. V. Galkin, O. Yu. Characteristics of enzyme-linked immunosorbent assay for detection of IgG antibodies specific to Chlamidia trachomatis heat shock protein (HSP-60) / O. Yu. Galkin, A. B. Besarab, T. N. Lutsenko. - P.22-30 Virych, P. A. Effect of 3-substituted 1,4-benzodiazepin-2-ones on bradykinin-induced smooth muscle contraction / P. A. Virych [et al.]. - P.31-37 Інші автори: Shelyuk O. V., Kabanova T. A., Khalimova E. I., Martynyuk V. S., Pavlovsky V. I., Andronati S. A. Minchenko, O. H. Inhibition of IRE1 modifies hypoxic regulation of G6PD, GP1, TKT, TALDO1, PGLS and RPIA genes expression in U87 glioma cells / О. Н. Minchenko [et al.]. - P.38-49 Інші автори: Garmash I. A., Minchenko D. O., Kuznetsova A. Y., Ratushna O. O. Holota, Yu. V. Fecal and short-chain fatty acids at different time points after ceftriaxone administration in rats / Yu. V. Holota [et al.]. - P.50-58 Інші автори: Holubenko O. O., Ostapchuk A. M., Serhiychuk T. M., Zakordonets L. V., Tolstanova G. M. Pismenetskaya, I. U. Serum glycomarkers of endoplasmic reticulum and lysosomal-endosomal system stress in human healthy aging and diseases / I. U. Pismenetskaya, T. D. Butters. - P.59-70 Suleimanova, R. R. Age-related changes of phospholipids in sterlet liver and dorsal muscles / R. R. Suleimanova [et al.]. - P.71-75 Інші автори: Hudz E. A., Melnychuk D. O., Kalachniuk L. H. Rudnytska, M. V. Effect of preparations Methyure and Ivine on Ca2+-ATPases activity in plasma and vacuolar membrane of corn seedling roots under salt stress conditions / M. V. Rudnytska, T. A. Palladina. - P.76-81 Lutsenko, T. N. Validation of biological activity testing procedure of recombinant human interleukin-7 / T. N. Lutsenko, M. V. Kovalenko, O. Yu. Galkin. - P.82-89 Виноградова, Р. П. Науково-практична діяльність відділу біохімії м’язів Інституту біохімії ім. О. В. Палладіна НАН України / Р. П. Виноградова, В. М. Данилова, С. П. Юрасова. - P.90-101 Є примірники у відділах: Прим. 1 (вільний) |
Galkin, O. Yu. Characteristics of enzyme-linked immunosorbent assay for detection of IgG antibodies specific to Chlamidia trachomatis heat shock protein (HSP-60) / O. Yu. Galkin, A. B. Besarab, T. N. Lutsenko // The Ukrainian Biochemical Journal. - 2017. - Том 89, N 1. - P22-30 MeSH-головна: CHLAMYDIA TRACHOMATIS -- CHLAMYDIA TRACHOMATIS (иммунология) АНТИТЕЛ ОБРАЗОВАНИЕ -- ANTIBODY FORMATION (иммунология) Анотація: The goal of this work was to study sensitivity and specificity of the developed ELISA set for the identification of IgG antibodies against Chlamydia trachomatis HSP-60 (using biotinylated tyramine-based signal amplification system). The study was conducted using a panel of characterized sera, as well as two reference ELISA sets of similar purpose. According to the results of ELISA informative value parameters, the ELISA we have developed showed the highest specificity and sensitivity parameters (no false negative or false positive results were registered). In 4 out of 15 intralaboratory panel serum samples initially identified as negative, anti-HSP-60 IgG-antibodies test result in reference ELISA sets upon dilution changed from negative to positive. The nature of titration curves of false negative sera and commercial monoclonal antibodies А57-В9 against C. trachomatis HSP-60 after incubation for 24 h was indicative of the presence of anti-idiotypic antibodies in these samples. Upon sera dilution, idiotypic-anti-idiotypic complexes dissociated, which caused the change of test result. High informative value of the developed ELISA set for identification of IgG antibodies against C. trachomatis HSP-60 has been proven. Anti-idiotypic antibodies possessing C. trachomatis anti-HSP-60 activity and being one of the causes of false negative results of the relevant ELISA-based tests have been identified in blood sera of individuals infected with chlamydial genitourinary infection agents Дод.точки доступу: Besarab, A. B. Lutsenko, T. N. |
Lutsenko, T. N. Validation of biological activity testing procedure of recombinant human interleukin-7 / T. N. Lutsenko, M. V. Kovalenko, O. Yu. Galkin // The Ukrainian Biochemical Journal. - 2017. - Том 89, N 1. - P82-89 MeSH-головна: ИНТЕРЛЕЙКИН-7 -- INTERLEUKIN-7 (фармакокинетика) Анотація: Validation procedure for method of monitoring the biological activity of reсombinant human interleukin-7 has been developed and conducted according to the requirements of national and international recommendations. This method is based on the ability of recombinant human interleukin-7 to induce proliferation of T lymphocytes. It has been shown that to control the biological activity of recombinant human interleukin-7 peripheral blood mononuclear cells (PBMCs) derived from blood or cell lines can be used. Validation characteristics that should be determined depend on the method, type of product or object test/measurement and biological test systems used in research. The validation procedure for the method of control of biological activity of recombinant human interleukin-7 in peripheral blood mononuclear cells showed satisfactory results on all parameters tested such as specificity, accuracy, precision and linearity Дод.точки доступу: Kovalenko, M. V. Galkin, O. Yu. |