Шифр: КУ53/2017/5/2 Журнал 2017г. т.5 N 2 . - 54.85, р. Хоменко, В. І. Роль HLA-типування в трансплантації гемопоетичних стовбурових клітин / В. І. Хоменко. - С.154-158. - Бібліогр.: в кінці ст. Khomenko, V. I. The role of HLA-typing in transplantation of hematopoietic stem cells / V. I. Khomenko. - С.159-163. - Bibliogr. at the end of the art. Волкова, Н. О. Порівняльне дослідження впливу bFGF та плазми, збагаченої факторами росту, на кріоконсервовані мультипотентні мезенхімальні стромальні клітини з кісткового мозку та сухожильної тканини щурів / Н. О. Волкова, М. С. Юхта, А. М. Гольцев. - С.164-169. - Бібліогр.: в кінці ст. Volkovа, N. A. Comparative study of the effect of bFGF and plasma rich in growth factors on cryopreserved mulipotent mesenhymal stromal cells from bone marrow and tendon of rats / N. A. Volkovа, M. S. Yukhta, A. N. Goltsev. - С.170-175. - Bibliogr. at the end of the art. Лабунець, І. Ф. Вплив екзогенного лейкемія-інгібіторного фактору на репаративну регенерацію периферичного нерва у мишей / І. Ф. Лабунець [та ін.]. - С.176-181. - Бібліогр.: в кінці ст. Другие авторы: Демидчук А. С., Шамало С. М., Утко Н. О., Родніченко А. Є., Римар С. Ю., Чайковський Ю. Б., Бутенко Г. М. Labunets, I. F. Effect of exogenous leukemia inhibitory factor on reparative regeneration of the peripheral nerve in mice / I. F. Labunets [та ін.]. - С.182-187. - Bibliogr. at the end of the art. Другие авторы: Demydchuk A. S., Shamalo S. M., Utko N. O., Rodnichenko A. E., Rymar S. Yu., Chaikovsky Y. B., Butenko G. M. Родніченко, А. Є. Деякі біологічні властивості мультипотентних мезенхімальних стромальних клітин кісткового мозку та жирової тканини мишей лінії FVB/N / А. Є. Родніченко. - С.188-193. - Бібліогр.: в кінці ст. Rodnichenko, A. E. Certain biological properties of multipotent mesenchymal stromal cells from bone marrow and adipose tissue of FVB/N mice / A. E. Rodnichenko. - С.194-199. - Bibliogr. at the end of the art. Чайка, А. В. Черепно-мозговая травма: патогенез, экспериментальные модели, перспективы клеточной терапии / А. В. Чайка [та ін.]. - С.200-208. - Бібліогр.: в кінці ст. Другие авторы: Забенько Е. Ю., Лабунец И. Ф., Пивнева Т. А. Chayka, A. V. Traumatic brain injury: pathogenesis, experimental models, prospects of cell-based therapy / A. V. Chayka [та ін.]. - С.209-215. - Bibliogr. at the end of the art. Другие авторы: Zaben’ko Y. Y., Labunets I. F., Pivneva T. A. Тези науково-практичної конференції з міжнародною участю "Інноваційні напрями в генетичній та регенеративній медицині" (9-10 листопада 2017 р., м. Київ, Україна). Додаток. - С.216-267 Имеются экземпляры в отделах: всего 1 Свободны: 1 |
Rodnichenko, A. E. Certain biological properties of multipotent mesenchymal stromal cells from bone marrow and adipose tissue of FVB/N mice [Text] / A. E. Rodnichenko // Клітинна та органна трансплантологія. - 2017. - Том 5, N 2. - P194-199. - Bibliogr. at the end of the art. MeSH-главная: МЫШИ ИНБРЕДНЫХ ЛИНИЙ -- MICE, INBRED STRAINS СТРОМАЛЬНЫЕ КЛЕТКИ МЕЗЕНХИМНЫЕ -- MESENCHYMAL STROMAL CELLS (ультраструктура, физиология) БОЛЕЗНЬ, МОДЕЛИ НА ЖИВОТНЫХ -- DISEASE MODELS, ANIMAL КОСТНОГО МОЗГА КЛЕТКИ -- BONE MARROW CELLS (ультраструктура, физиология) ЖИРОВАЯ ТКАНЬ -- ADIPOSE TISSUE (ультраструктура, физиология) СРАВНИТЕЛЬНЫЕ ИССЛЕДОВАНИЯ -- COMPARATIVE STUDY ИММУНОМОДУЛЯЦИЯ -- IMMUNOMODULATION Аннотация: Multipotent mesenchymal stromal cells (MMSCs) are used for cell therapy of lesions of various genesis. The most widely used MMSCs are from two tissue sources: bone marrow and adipose tissue. The purpose of the work was to conduct a comparative assessment of the biological properties of murine bone marrow-derived and adipose tissue-derived MMSCs Methods. The culture of MMSCs was obtained from the bone marrow and adipose tissue of 6 months-old male FVB/N mice according to standard protocols. We performed phenotyping, directed osteogenic and adipogenic differentiation, analysis of immunomodulatory properties in vitro of obtained cell cultures. Results. The cultured MMSCs from bone marrow and adipose tissue express the typical stromal markers (CD44, CD73, CD90 and Sca-1). A distinctive feature of bone marrow cells cultures of the 2nd passage was the high level of the hematopoietic markers CD45 and CD117 expression. MMSCs from both tissue sources are capable of differentiation in the osteogenic and adipogenic directions. At the same time, there were differences in the differentiation in the osteogenic direction – adipose tissue-derived MMSCs had a lower osteogenic potential. MMSCs exhibit inhibitory effect on mitogen-induced proliferation of splenocytes in vitro, expression of which does not depend on tissue origin of the MMSCs with significant inhibition of mitogen-induced proliferation of splenocytes at addition of high doses of MMSCs. Conclusions. MMSCs of bone marrow and adipose tissue express a similar level of surface markers that are characteristic of cells with multipotent properties. They are capable to differentiating in osteo- and adipogenic direction with differences in the degree of mineralization of the extracellular matrix and exhibit immunomodulatory effects in vitro, regardless of tissue origin Свободных экз. нет |
Шифр: КУ53/2018/6/1 Журнал 2018г. т.6 N 1 Хоменко, В. І. Аналіз міжнародних та національних норм правової регламентації у сфері трансплантації тканин і клітин та можливості покращення нормативно-правового забезпечення трансплантації гемопоетичних стовбурових клітин в Україні / В. І. Хоменко. - С.6-21. - Бібліогр.: в кінці ст. Khomenko, V. I. Review of international and national principles of law in the field of tissue and cell transplantation and the approaches of improving the regulatory framework of hematopoietic stem cells transplantation in Ukraine / V. I. Khomenko. - С.22-37. - Bibliogr. at the end of the art. Квитницкая-Рыжова, Т. Ю. Влияние генной терапии PEI-pDNA комплексом, несущим ген препроинсулина человека, на структурные и ультраструктурные характеристики ряда органов при экспериментальном сахарном диабете у мышей разного возраста / Т. Ю. Квитницкая-Рыжова [та ін.]. - С.38-47. - Библиогр. в конце ст. Другие авторы: Луговской С. П., Клименко П. П., Михальский С. А., Хаблак Г. В., Топорова Е. К. Kvitnitskaya-Ryzhova, T. Yu. The effects of gene therapy with PEI-pDNA complex containing human preproinsulin gene on structural and ultrastructural characteristics of several organs in mice of different ages at experimental diabetes mellitus / T. Yu. Kvitnitskaya-Ryzhova [та ін.]. - С.48-56. - Bibliogr. at the end of the art. Другие авторы: Lugovskoy S. P., Klymenko P. P., Mykhalsky S. A., Khablak G. V., Toporova E. K. Лабунец, И. Ф. Влияние разных доз тимулина in vivo и in vitro на некоторые биологические свойства мультипотентных мезенхимальных стромальных клеток костного мозга у мышей разных линий / И. Ф. Лабунец, А. Е. Родниченко. - С.58-65. - Библиогр. в конце ст. Labunets, I. F. The effects of different doses of thymulin in vivo and in vitro on some biological properties of bone marrow-derived multipotent mesenchymal stromal cells in mice of different strains / I. F. Labunets, A. E. Rodnichenko. - С.66-73. - Bibliogr. at the end of the art. Калмикова, О. О. Вплив різних режимів введення мелатоніну на диференціацію та функціональний стан бурих адипоцитів in vivo / О. О. Калмикова, М. Е. Дзержинський. - С.74-79. - Бібліогр.: в кінці ст. Kalmukova, O. O. The effects of different time of melatonin administration on differentiation and functional status of the brown adipocytes in vivo / O. O. Kalmukova, M. E. Dzerzhinsky. - С.80-85. - Bibliogr. at the end of the art. Родніченко, А. Є. Відтворення токсичної купризонової моделі демієлінізації в системі in vitro / А. Є. Родніченко. - С.86-92. - Бібліогр.: в кінці ст. Rodnichenko, A. E. Implementation of a toxic cuprizone model of demyelination in vitro / A. E. Rodnichenko. - С.93-98. - Bibliogr. at the end of the art. Имеются экземпляры в отделах: всего 1 Свободны: 1 |
Labunets, I. F. The effects of different doses of thymulin in vivo and in vitro on some biological properties of bone marrow-derived multipotent mesenchymal stromal cells in mice of different strains [Текст] / I. F. Labunets, A. E. Rodnichenko // Клітинна та органна трансплантологія. - 2018. - Том 6, N 1. - С. 66-73. - Bibliogr. at the end of the art. Аннотация: A promising source for cell therapy are multipotent mesenchymal stromal cells (MMSC), whose biological properties can change at immune dysfunction of central genesis. The purpose of the study is to investigate the ability of murine bone marrow-derived MMSC to colony formation, directed differentiation and immunosuppressive effect at different content of serum thymic factor (thymulin) in the body and in the cell culture in mice of different strains. Materials and methods. MMSCs cultures were obtained from the bone marrow of FVB/N and 129/Sv mice. We performed the study of clonogenic ability of MMSCs, phenotyping, assessment of the osteogenic and adipogenic potential, estimation of immunomodulating properties of MMSCs after thymectomy or addition of the thymulin in the culture in vitro. Results. It was found that the level of thymulin in the control mice of the FVB/N strain is higher than in 129/Sv mice. The ability of bone marrow progenitor cells to colony formation in control and thymectomized mice depends on their genotype. When adding thymulin at concentrations of 1 ng/mL and 10 ng/mL to the bone marrow cells culture of both strains thymectomized mice, the change in the number of colony-forming unit fibroblasts was observed only with the addition of a high dose of the hormone. Thymectomy in FVB/N mice leads to an increase in the differentiation of MMSCs in the osteogenic direction and to a decrease in adipogenic differentiation, which is restored after adding thymulin to the cell culture. In thymectomized 129/Sv mice, there was a decrease in osteogenic differentiation and recovery after incubation of cells with thymulin. The immunosuppressive effect of bone marrow MMSCs was established in mice of both strains. In this case, strain-dependent differences in this effect are manifested in the degree of immunosuppression. Conclusion. The ability of bone marrow MMSC of the FVB/N and 129/Sv mice to colony formation, directed differentiation and immunosuppressive effect at different content of thymulin in the body or in cell culture is related with the thymus function and changes after thymectomy. The response of the bone marrow MMSCs to the thymectomy depends on the genotype of the mice. It has been shown that thymulin has a recover effect on the reduced clonogenic and osteogenic potential of the bone marrow-derived MMSCs in mice of both strains and the adipogenic potential in 129/Sv mice. Доп.точки доступа: Rodnichenko, A. E. Свободных экз. нет |
Rodnichenko, A. E. Implementation of a toxic cuprizone model of demyelination in vitro [Текст] / A. E. Rodnichenko // Клітинна та органна трансплантологія. - 2018. - Том 6, N 1. - С. 93-98. - Bibliogr. at the end of the art. Аннотация: The relevance of the use of adequate experimental models to study the mechanisms of the development of multiple sclerosis and the effects of drugs on the remyelination of nerve fibers due to the prevalence of this disease, which affects mostly young people. The purpose of the study was to adjustment a toxic cuprizone model in vitro, with confirming the processes of demyelination of nerve fibres Materials and methods. To study the features of the processes of myelination and demyelination of neurons axons, the culture of dissociated cerebellar cells of 7-day old newborn FVB/N mice was used. To detect myelin sheaths, we used a histochemical staining with Sudan Black B dye. To identify oligodendrocytes, their culture was stained by immunocytochemistry for Olig2 marker. Results. Developing a model of toxic demyelination, we determined the density of the cerebellar cell culture, selected conditions for the cultivation of dissociated cerebellar cells, and the survival of the dissociated culture of the cerebellum until the 26-28th day of cultivation was achieved. Using histochemical staining, the presence of myelination and demyelination processes in vitro was confirmed. Using immunocytochemical staining, it has been shown that the neurotoxin cuprizone results in a decrease of Olig2-positive oligodendrocytes, when it is applied on the 18th day of cultivation of the dissociated cerebellar cells culture. Conclusion. The conditions for the cultivation of murine dissociated cerebellar cells were selected, the presence of myelination and demyelination processes in vitro was confirmed; a decrease in the number of Olig2-positive cells when the demyelination agent was added to the culture on the 18th day was shown. Свободных экз. нет |
Шифр: КУ53/2018/6/2 Журнал 2018г. т.6 N 2 Хоменко, В. І. Трансплантація гемопоетичних стовбурових клітин: системно-історичний аспект / В. І. Хоменко. - С.112-117. - Бібліогр.: в кінці ст. Khomenko, V. I. Transplantation of hematopoietic stem cells: historical aspects / V. I. Khomenko. - С.118-123. - Bibliogr. at the end of the art. Волкова, Н. О. Потенціювання кріоконсервованих мультипотентних мезенхімальних стромальних клітин з жирової тканини щурів фактором BMP-12 in vitro для терапії тендопатії / Н. О. Волкова, М. С. Юхта, А. М. Гольцев. - С.124-130. - Бібліогр.: в кінці ст. Volkova, N. O. Potentiation of cryopreserved rat adipose-derived multipotent mesenchymal stromal cells by BMP-12 in vitro for the treatment of tendinopathy / N. O. Volkova, M. S. Yukhta, A. M. Goltsev. - С.131-136. - Bibliogr. at the end of the art. Лабунець, І. Ф. Нейропротекторний ефект мелатоніну у мишей із токсичною купризоновою моделлю демієлінізації і можливі шляхи його реалізації / І. Ф. Лабунець [та ін.]. - С.138-144. - Бібліогр.: в кінці ст. Другие авторы: Родніченко А. Є., Мельник Н. О., Утко Н. О. Labunets, I. F. Neuroprotective effect of melatonin in mice with toxic cuprizone model of demyelination and possible pathways of its realization / I. F. Labunets [et. al.]. - С.145-151. - Bibliogr. at the end of the art. Другие авторы: Rodnichenko A. E., Melnyk N. O., Utko N. O. Нікольська, К. І. Вплив трансплантації мультипотентних стромальних клітин тимуса на імунну систему і виживаність летально опромінених мишей / К. І. Нікольська. - С.152-157. - Бібліогр.: в кінці ст. Nikolska, K. I. The effects of the transplantation of thymus-derived multipotent stromal cells on the immune system and survival of lethally irradiated mice / K. I. Nikolska. - С.158-163. - Bibliogr. at the end of the art. Демченко, Д. Л. Вплив трансплантації клітин кісткового мозку, індукованих контактом з мультипотентними стромальними клітинами тимуса, на імунну систему мишей в умовах її регенерації після введення циклофосфану / Д. Л. Демченко. - С.164-169. - Бібліогр.: в кінці ст. Demchenko, D. L. The effect of transplantation of bone marrow cells induced by the contact with thymus-derived multipotent stromal cells on the immune system of mice, regenerating after cyclophosphamide treatment / D. L. Demchenko. - С.170-175. - Bibliogr. at the end of the art. Родніченко, А. Є. Дослідження ремієлінізуючого ефекту лейкемія-інгібіторного фактора та мелатоніну на токсичній купризоновій моделі демієлінізації культури клітин мозочка мишей in vitro / А. Є. Родніченко, І. Ф. Лабунець. - С.176-181. - Бібліогр.: в кінці ст. Rodnichenko, A. E. The study of the remyelinating effect of leukemia inhibitory factor and melatonin on the toxic cuprizone model of demyelination of murine cerebellar cells culture in vitro / A. E. Rodnichenko, I. F. Labunets. - С.182-187. - Bibliogr. at the end of the art. Кирик, В. М. 3D культивування мультипотентних мезенхімальних стромальних клітин жирової тканини мишей в гідрогелі з карбомеру 974P / В. М. Кирик [та ін.]. - С.188-194. - Бібліогр.: в кінці ст. Другие авторы: Кучук О. В., Мамчур А. А., Устименко А. М., Луценко Т. М., Цупиков О. М., Яценко К. В., Скибо Г. Г., Білько Д. І., Білько Н. М. Kyryk, V. M. 3D culture of murine adipose-derived multipotent mesenchymal stromal cells in hydrogel based on carbomer 974P / V. M. Kyryk [et. al.]. - С.195-201. - Bibliogr. at the end of the art. Другие авторы: Kuchuk O. V., Mamchur A. A., Ustymenko A. M., Lutsenko T. M., Tsupykov O. M., Yatsenko K. V., Skibo G. G., Bilko D. I., Bilko N. M. Имеются экземпляры в отделах: всего 1 Свободны: 1 |
Neuroprotective effect of melatonin in mice with toxic cuprizone model of demyelination and possible pathways of its realization [Текст] / I. F. Labunets [та ін.] // Клітинна та органна трансплантологія. - 2018. - Том 6, N 2. - С. 145-151. - Bibliogr. at the end of the art. Аннотация: The search for tools that increase the effectiveness of cell therapy of demyelinating pathology is relevant. They may be preparations that affect the pathogenetic factors of this pathology, in particular, the pineal hormone melatonin The PURPOSE of the study is to evaluate the involvement of immune system and antioxidant defense in the implementation of the protective effects of melatonin on morphofunctional disorders in the central nervous system induced by neurotoxin cuprizone MATERIALS AND METHODS. The toxic demyelination model was induced on 129/Sv mice at the age of 3-5 months by adding cuprizone to food for 3 weeks. Since the 7"1 day of cuprizone administration, melatonin was injected intraperitoneally at 6p.m. daily, at a dose of 1 mg/kg. In the brain of mice, the proportion of CDS\ Nestin* cells and phagocytic macrophages, the content of malondialdehyde and the activity of antioxidant enzymes was determined. Blood serum was tested for thymic hormone thymulin levels. In the animals, we evaluated the structure of the brain and spinal cord neurons by Nissl staining of histological sections as well as analyzed behavioural reactions in the «open field» test RESULTS. In the brain of the mice receiving cuprizone, the proportion of CD3* and Nestin* cells, active macrophages and malondialdehyde content increased, glutathione peroxidase and glutathione reductase levels decreased. In the brain and spinal cord of the mice with a cuprizone diet, the proportion of altered neurons increased, and motor and emotional activity decreased. The administration of melatonin results in a decrease in the relative number of CD3* cells, active macrophages and malondialdehyde content, increased activity of glutathione peroxidase, glutathione reductase and level of thymulin. In such mice, the proportion of unchanged neurons increases as the number of Nestin* cells decreases and behavioural responses are also improved CONCLUSIONS. The neuroprotective effect of melatonin in demyelinating pathology is realized through the factors of the immune system and oxidative stress. The results may be useful in the development of new biotechnological approaches to the treatment of demyelinating pathology, in particular, multiple sclerosis Доп.точки доступа: Labunets, I. F. Rodnichenko, A. E. Melnyk, N. O. Utko, N. O. Свободных экз. нет |
Rodnichenko, A. E. The study of the remyelinating effect of leukemia inhibitory factor and melatonin on the toxic cuprizone model of demyelination of murine cerebellar cells culture in vitro [Текст] / A. E. Rodnichenko, I. F. Labunets // Клітинна та органна трансплантологія. - 2018. - Том 6, N 2. - С. 182-187. - Bibliogr. at the end of the art. Аннотация: The cuprizone model of toxic demyelination in vitro is widely used to study de- and remyelination in the CNS, as well as to address the issues of finding potential compounds that affect myelination of neuron axons THE AIM OF THE STUDY was to investigate the role of recombinant human leukemia inhibitory factor (rhLIF) and melatonin in remyelination, using the cuprizone demyelination model in vitro METHODS. To study the features of the demyelination and remyelination processes of neuronal axons, the culture of dissociated cerebellar cell culture of the 7-day-old FVB/N lineage mice was used. To detect the myelin sheaths, a histochemical staining with a Sudan Black В was used. To identify oligodendrocytes, immunocytochemical staining of 28-30-day old cerebellar cells cultures for oligodendrocytes marker 0lig2 was performed RESULTS. The direct effect of the demyelinating factor of cuprizone and remyelination agents (rhLIF and melatonin) on oligodendrocytes in vitro was confirmed. The remyelinating effect of LIF and melatonin on the restoration of myelination processes in dissociated cerebellar cell culture using histochemical and immunocytochemical staining has been revealed CONCLUSIONS. Cuprizone-induced demyelination in vitro is associated with the death of 0lig2* oligodendrocytes and loss of myelin formation. rhLIF and melatonin prevented the loss of oligodendrocytes and, consequently, reduced the destruction of myelin membranes Доп.точки доступа: Labunets, I. F. Свободных экз. нет |
Шифр: КУ53/2019/7/1 Журнал 2019г. т.7 N 1 . - 65.26, р. Волкова, Н. О. Оцінка ефективності застосування біополімерів при низькотемпературному консервуванні фрагментів звитих канальців сім’яників щурів препубертатного віку / Н. О. Волкова [та ін.]. - С.6-11. - Бібліогр.: в кінці ст. Другие авторы: Юхта М. С., Чернишенко Л. Г., Степанюк Л. В., Сокіл Л. В., Гольцев А. М. Volkova, N. O. The effectiveness of biopolymers application for cryopreservation of the fragments of convoluted seminiferous tubules of prepubertal rat’s testis / N. O. Volkova [та ін.]. - С.12-17. - Bibliogr. at the end of the art. Другие авторы: Yukhta M. S., Chernyschenko L. G., Stepaniuk L. V., Sokil L. V., Goltsev A. M. Лабунець, І. Ф. Ефекти інтерлейкіну-10 та фактора росту фібробластів-2 у мишей із токсичною купризоновою моделлю демієлінізації / І. Ф. Лабунець [та ін.]. - С.18-24. - Бібліогр.: в кінці ст. Другие авторы: Родніченко А. С., Утко Н. О., Пантелеймонова Т. М., Похоленко Я. О., Літошенко 3. Л., Бутенко Г. М. Labunets, I. F. The effects of interleukin-10 and fibroblasts growth factor 2 in mice with toxic cuprizone model of demyelination / I. F. Labunets [та ін.]. - С.25-31. - Bibliogr. at the end of the art. Другие авторы: Rodnichenko A. E., Utko N. A., Panteleimonova Т. М., Pokholenko Ya. O., Litoshenko Z. L., Butenko G. M. Гольцев, А. М. Імунокоригуюча дія фетальних нервових клітин мишей при розвитку хвороби «трансплантат проти хазяїна» / А. М. Гольцев [та ін.]. - С.32-39. - Бібліогр.: в кінці ст. Другие авторы: Бабенко Н. М., Гаєвська Ю. О., Дубрава Т. Г., Луценко О. Д., Бондарович М. О. Goltsev, A. M. Immunoregulatory effect of mouse fetal neural cells on the graft-versus- host disease / A. M. Goltsev [та ін.]. - С.40-46. - Bibliogr. at the end of the art. Другие авторы: Babenko N. M., Gaevska Yu. O., Dubrava T. G., Lutsenko O. D., Bondarovych M. O. Демченко, Д. Л. Вплив ко-трансплантації гемопоетичних стовбурових клітин кісткового мозку та мультипотентних стромальних клітин тимуса на імунну систему мишей в умовах її регенерації після введення циклофосфану / Д. Л. Демченко, І. С. Нікольський. - С.48-53. - Бібліогр.: в кінці ст. Demchenko, D. L. The effects of co-transplantation of bone marrow hematopoietic stem cell and thymic multipotent stromal cells on the immune system of mice during its regeneration after cyclophosphamide treatment / D. L. Demchenko, I. S. Nikolskiy. - С.54-59. - Bibliogr. at the end of the art. Насадюк, Х. М. Персоналізована медицина: реалії та перспективи / Х. М. Насадюк, О. Я. Скляров. - С.60-62. - Бібліогр.: в кінці ст. Nasadyuk, C. M. Personalized medicine: current status and prospects / С. М. Nasadyuk, O. Ya. Sklyarov. - С.63-65. - Bibliogr. at the end of the art. Тези 6-го з’їзду Українського товариства клітинної біології (18-21 червня 2019 р., м. Яремче, Україна) Секція “Біологія стовбурових та спеціалізованих клітин і тканин”. - С.66-85 Имеются экземпляры в отделах: всего 1 Свободны: 1 |
The effects of interleukin-10 and fibroblasts growth factor 2 in mice with toxic cuprizone model of demyelination [Текст] / I. F. Labunets [та ін.] // Клітинна та органна трансплантологія. - 2019. - Том 7, N 1. - С. 25-31. - Bibliogr. at the end of the art. Аннотация: Cytokines and growth factors exhibit neurotropic, anti-inflammatory and immunomodulatory properties, and therefore can affect the functioning of the nervous system at demyelinating disorders. Purpose. To identify changes in T-lymphocytes, macrophages, oxidative stress and antioxidant defence factors, endocrine thymus function in the brain and behaviour of mice receiving neurotoxin cuprizone and recombinant human proteins: interleukin-10 (rhIL-10) and fibroblast growth factor (rhFGF-2). Materials and methods. 4-6-month-old 129/Sv mice received cuprizone with food every day for 3 weeks. From the 7th day of cuprizone diet, they received different doses of rhIL-10 and rhFGF-2. The content of СD3+ Т-cells, macrophages, malondialdehyde, activity of antioxidant enzymes in the brain and the level of thymulin in the blood were determined. Behavioural reactions were assessed in the “open field” test. Results. In the brain of mice receiving cuprizone and rhIL-10, there was a decrease in the number of СD3+ Т-cells and the activity of macrophages, which significantly increased under the influence of the toxin. After cytokine injection, the activity of superoxide dismutase, catalase and glutathione peroxidase increased significantly in the brain, as well as the level of thymulin in the blood. Motor, emotional and exploratory activity of mice, significantly suppressed by the cuprizone, was increased after the introduction of rhIL-10. The effect of rhIL-10 on the test parameters is more pronounced at a dose of 5 μg/kg than 50 μg/kg. After injection of rhFGF-2 in the mice with cuprizone diet, there is a decrease in the activity of brain macrophages and an increase of the level of thymulin in the blood depending on the dose of this factor; the motor activity of these animals increased regardless of the rhFGF-2 dose. Conclusion. The injections of rhIL-10 and rhFGF-2 provide dose-dependent positive effects on the pathogenetic factors of experimental demyelinating pathology, as well as the functional state of the nervous system. Whereas, the injections of rhIL-10 have more pronounced effects. Доп.точки доступа: Labunets, I. F. Rodnichenko, A. E. Utko, N. A. Panteleimonova, Т. М. Pokholenko, Ya. O. Litoshenko, Z. L. Butenko, G. M. Свободных экз. нет |
Шифр: ЕУ12/2017/39/3 Журнал 2017г. Т. 39 № 3 . - 34.76, р. Chekhun, V. F. On the 40th anniversary of the international symposium “The role of stem cells in leukemo-and carcinogenesis»: in Kyiv again / V. F. Chekhun. - С.162-163 Kalynychenko, T. O. Umbilical cord blood banking in the worldwide hematopoietic stem cell transplantation system: perspectives for Ukraine / T. O. Kalynychenko. - С.164-170. - Bibliogr. at the end of the art. Vasyliev, R. G. Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications / R. G. Vasyliev [та ін.]. - С.171-180. - Bibliogr. at the end of the art. Другие авторы: Rodnichenko A. E., Gubar O. S., Zlatska A. V., Gordiienko I. M., Novikova S. N., Zubov D. O. Kozub, M. M. Comparison of various tissue and cell therapy approaches when restoring ovarian, hepatic and kidney’s function after chemotherapy-induced ovarian failure / M. M. Kozub [та ін.]. - С.181-185. - Bibliogr. at the end of the art. Другие авторы: Prokopiuk V. Y., Skibina K. R., Prokopiuk О. V., Kozub N. I. Gubar, O. S. Postnatal extra-embryonic tissues as a source of multiple cell types for regenerative medicine applications / O. S. Gubar [та ін.]. - С.186-190. - Bibliogr. at the end of the art. Другие авторы: Rodnichenko A. E., Vasyliev R. G., Zlatska A. V., Zubov D. O. Vasyliev, R. G. Tissue-engineered bone for treatment of combat related limb injuries / R. G. Vasyliev [та ін.]. - С.191-196. - Bibliogr. at the end of the art. Другие авторы: Oksymets V. M., Rodnichenko A. E., Zlatska A. V., Gubar O. S., Gordiienko I. M., Zubov D. O. Zlatska, A. V. Endometrial stromal cells: isolation, expansion, morphological and functional properties / A. V. Zlatska [та ін.]. - С.197-202. - Bibliogr. at the end of the art. Другие авторы: Rodnichenko A. E., Gubar O. S., Zubov D. O., Novikova S. N., Vasyliev R. G. Chekhun, V. F. Association of CD44+CD24-/low with markers of aggressiveness and plasticity of cell lines and tumors of patients with breast cancer / V. F. Chekhun [та ін.]. - С.203-211. - Bibliogr. at the end of the art. Другие авторы: Lukianova N. Y., Chekhun S. V., Bezdieniezhnykh N. O., Zadvomiy T. V., Borikun T. V., Polishchuk L. Z., Klyusov O. M. Karlitepe, A. Anti-cancer efficiency of natural killer cells differentiated from human adipose tissue-derived mesenchymal stem cells and transfected with miRNA150 / A. Karlitepe [та ін.]. - С.212-218. - Bibliogr. at the end of the art. Другие авторы: Kabadayi H., Vatansever S., Gurdal M., Gunduz C., Ercan G. Lisyaniy, N. I. Content of stem tumor CD133+ cells in brain neoplasms of different histological type / N. I. Lisyaniy [та ін.]. - С.219-223. - Bibliogr. at the end of the art. Другие авторы: Stanetskaya D. N., Lisyaniy A. N., Belskaya L. N. Ryspayeva, D. E. Are CD44+CD24- cells the assumed cancer stem cells in breast cancer? / D. E. Ryspayeva [та ін.]. - С.224-228. - Bibliogr. at the end of the art. Другие авторы: Smolanka I. I., Dudnichenko A. S., Lyashenko A. A., Grinevich Yu. A., Gurianov V. G., Koshubarova M. V., Seleznev A. A. Bezdieniezhnykh, N. O. Scientific-practical and legal problems of implementation of the personalized medicine / N. O. Bezdieniezhnykh, V. V. Reznikova, O. V. Rossylna. - С.229-233 Normal and cancer stem cells: discovery diagnosis and therapy international scientific conference. R. E. Kavetsky Institute of Experimental Pathology Oncology and Radiobiology National Academy of Sciences of Ukraine Kyiv October 5–6 2017. - С.234-256 Имеются экземпляры в отделах: всего 1 Свободны: 1 |
Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications [Текст] / R. G. Vasyliev [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 171-180. - Bibliogr. at the end of the art. MeSH-главная: СТВОЛОВЫХ КЛЕТОК ИССЛЕДОВАНИЕ -- STEM CELL RESEARCH СТРОМАЛЬНЫЕ КЛЕТКИ МЕЗЕНХИМНЫЕ -- MESENCHYMAL STROMAL CELLS (физиология) НЕРВНЫЙ ГРЕБЕШОК -- NEURAL CREST (ультраструктура, физиология) ВОЛОСЯНОЙ ФОЛЛИКУЛ -- HAIR FOLLICLE (анатомия и гистология, ультраструктура) ЧЕЛОВЕК -- HUMANS (анатомия и гистология) ЦИТОФЛЮОРОМЕТРИЯ -- FLOW CYTOMETRY (использование, тенденции) МОРФОЛОГИЧЕСКИЕ И МИКРОСКОПИЧЕСКИЕ ПОКАЗАТЕЛИ -- MORPHOLOGICAL AND MICROSCOPIC FINDINGS ФЕНОТИП -- PHENOTYPE СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS Аннотация: The purpose of this work was to obtain, multiply and characterize the adult neural crest-derived multipotent stem cells from human hair follicle for their further clinical use. Materials and Methods: Adult neural crest-derived multipotent stem cells were obtained from human hair follicle by explant method and were expanded at large-scale up to a clinically significant number. The resulted cell cultures were examined by flow cytometry and immunocytochemical analysis. Their clonogenic potential, ability to self-renewal and directed multilineage differentiation were also investigated. Results: Cell cultures were obtained from explants of adult human hair follicles. Resulted cells according to morphological, phenotypic and functional criteria satisfied the definition of neural crest-derived multipotent stem cells. They had the phenotype Sox2⁺Sox10⁺Nestin⁺CD73⁺CD90⁺CD105⁺CD140a⁺CD 140b⁺CD146⁺CD166⁺CD271⁺CD349⁺ CD34⁻CD45⁻CD56⁻HLA⁻DR⁻, showed high clonogenic potential, ability to self-renewal and directed differentiation into the main derivatives of the neural crest: neurons, Schwann cells, adipocytes and osteoblasts. Conclusion: The possibility of a large-scale expansion of adult neural crest-derived multipotent stem cells up to 40–200·106 cells from minimal number of hair follicles with retention of their phenotype and functional properties are the significant step towards their translation into the clinical practice Доп.точки доступа: Vasyliev, R. G. Rodnichenko, A. E. Gubar, O. S. Zlatska, A. V. Gordiienko, I. M. Novikova, S. N. Zubov, D. O. Свободных экз. нет |
Postnatal extra-embryonic tissues as a source of multiple cell types for regenerative medicine applications [Текст] / O. S. Gubar [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 186-190. - Bibliogr. at the end of the art. MeSH-главная: СТРОМАЛЬНЫЕ КЛЕТКИ МЕЗЕНХИМНЫЕ -- MESENCHYMAL STROMAL CELLS (ультраструктура) ХОРИОНАЛЬНЫЕ ВОРСИНКИ -- CHORIONIC VILLI (эмбриология) ПЛОДА КРОВЬ -- FETAL BLOOD АЛЛОТРАНСПЛАНТАЦИЯ -- TRANSPLANTATION, HOMOLOGOUS (использование, методы, тенденции) ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL Аннотация: We aimed to isolate and characterize the cell types which could be obtained from postnatal extra-embryonic tissues. Materials and Methods: Fresh tissues (no more than 12 h after delivery) were used for enzymatic or explants methods of cell isolation. Obtained cultures were further maintained at 5% oxygen. At P3 cell phenotype was assessed by fluorescence-activated cell sorting, population doubling time was calculated and the multilineage differentiation assay was performed. Results: We have isolated multiple cell types from postnatal tissues. Namely, placental mesenchymal stromal cells from placenta chorionic disc, chorionic membrane mesenchymal stromal cells (ChM-MSC) from free chorionic membrane, umbilical cord MSC (UC-MSC) from whole umbilical cord, human umbilical vein endothelial cells (HUVEC) from umbilical vein, amniotic epithelial cells (AEC) and amniotic MSC (AMSC) from amniotic membrane. All isolated cell types displayed high proliferation rate together with the typical MSC phenotype: CD73⁺CD90⁺CD105⁺CD146⁺CD166⁺CD34⁻CD45⁻HLA⁻DR⁻. HUVEC constitutively expressed key markers CD31 and CD309. Most MSC and AEC were capable of osteogenic and adipogenic differentiation. Conclusion: We have shown that a wide variety of cell types can be easily isolated from extra-embryonic tissues and expanded ex vivo for regenerative medicine applications. These cells possess typical MSC properties and can be considered an alternative for adult MSC obtained from bone marrow or fat, especially for allogeneic use Доп.точки доступа: Gubar, O. S. Rodnichenko, A. E. Vasyliev, R. G. Zlatska, A. V. Zubov, D. O. Свободных экз. нет |
Tissue-engineered bone for treatment of combat related limb injuries [Текст] / R. G. Vasyliev [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 191-196. - Bibliogr. at the end of the art. MeSH-главная: СЛОЖНЫЕ ТКАНЕВЫЕ АЛЛОТРАНСПЛАНТАТЫ -- COMPOSITE TISSUE ALLOGRAFTS (трансплантация) КОСТНОГО МОЗГА ТРАНСПЛАНТАЦИЯ -- BONE MARROW TRANSPLANTATION (методы, тенденции) ВОЕННОСЛУЖАЩИЕ -- MILITARY PERSONNEL ПЕРЕЛОМЫ КОСТИ -- FRACTURES, BONE (патофизиология, этиология) СТВОЛОВЫХ КЛЕТОК МЕЗЕНХИМНЫХ ТРАНСПЛАНТАЦИЯ -- MESENCHYMAL STEM CELL TRANSPLANTATION (использование, методы, тенденции) СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS Аннотация: Based on our preliminary positive clinical results with use of cultured bone marrow-derived multipotent mesenchymal stem/stromal cells in traumatology, our aim was to develop living three-dimensional tissue-engineered bone equivalent transplantation technology for restoration of critical sized bone defects caused by combat related high energy trauma. Materials and Methods: To fabricate bone equivalent we used devitalized allogeneic bone scaffolds (blocks and chips) seeded with cultured autologous cells: bone marrow-derived multipotent mesenchymal stem/stromal cells in mix with periosteal progenitor cells and endothelial progenitor cells. Quality/identity of cell cultures was assured by donor and cell culture infection screening (immunofluorescence assay, polymerase chain reaction), flow cytometry (cell phenotype), karyotyping (GTG banding), functional assays (colony forming units analysis, multilineage differentiation assay). Bone defect treatment with bone equivalent application was fully completed in 39 combat-injured with 42 defects. New bone formation was assessed by the radiographic examination. Results: Casualties were included in a treatment program an average of 10.1 months after injury, provided the ineffectiveness of conventional surgery methods. All cell type cultures had a normal karyotype and appropriate phenotype, differentiation potential and functional properties, ~30% colony forming units frequency and hadn’t any signs of cell senescence. The fluorescein diacetate/propidium iodide combined staining and histology analysis of graft samples before transplantation showed their regular seeding with viable cells. Pathomorphological analysis of bone equivalent specimens 3–6 months post-op revealed the active remodeling processes and immature bone tissue formation. Bone defect restoration was observed 5–6 months post-op. Conclusion: The developed biotechnology of living three-dimensional tissue-engineered bone equivalent transplantation with overall effectiveness 90.4% allows restoring the bone integrity, forming new bone tissue in a site of bone defect, and significantly reducing the rehabilitation period of a patient Доп.точки доступа: Vasyliev, R. G. Oksymets, V. M. Rodnichenko, A. E. Zlatska, A. V. Gubar, O. S. Gordiienko, I. M. Zubov, D. O. Свободных экз. нет |
Endometrial stromal cells: isolation, expansion, morphological and functional properties [Текст] / A. V. Zlatska [та ін.] // Экспериментальная онкология. - 2017. - Т. 39, № 3. - С. 197-202. - Bibliogr. at the end of the art. MeSH-главная: СТРОМАЛЬНЫЕ КЛЕТКИ -- STROMAL CELLS (ультраструктура, физиология) IN VITRO МЕТОДЫ -- IN VITRO TECHNIQUES (использование, тенденции) ЭНДОМЕТРИЙ -- ENDOMETRIUM (анатомия и гистология) КОЛОНИЕОБРАЗУЮЩИХ ЕДИНИЦ АНАЛИЗ -- COLONY-FORMING UNITS ASSAY (методы, тенденции) МЕДИЦИНА РЕГЕНЕРАТИВНАЯ -- REGENERATIVE MEDICINE (методы, организация и управление, тенденции) СТАТИСТИЧЕСКАЯ ОБРАБОТКА ДАННЫХ -- DATA INTERPRETATION, STATISTICAL ФОТОГРАФИЧЕСКИЕ СНИМКИ -- PHOTOGRAPHS Аннотация: We aimed to study biological properties of human endometrial stromal cells in vitro. Materials and methods: The endometrium samples (n = 5) were obtained by biopsy at the first phase of the menstrual cycle from women with endometrial hypoplasia. In all cases, a voluntary written informed consent was obtained from the patients. Endometrial fragments were dissociated by enzymatic treatment. The cells were cultured in DMEM/F12 supplemented with 10% FBS, 2 mМ L-glutamine and 1 ng/ml FGF-2 in a multi-gas incubator at 5% CO2 and 5% O2. At P3 the cells were subjected to immunophenotyping, multilineage differentiation, karyotype stability and colony forming efficiency. The cell secretome was assessed by BioRad Multiplex immunoassay kit. Results: Primary population of endometrial cells was heterogeneous and contained cells with fibroblast-like and epithelial-like morphology, but at P3 the majority of cell population had fibroblast-like morphology. The cells possessed typical for MSCs phenotype CD90+CD105+CD73+CD34-CD45-HLA-DR-. The cells also expressed CD140a, CD140b, CD146, and CD166 antigents; and were negative for CD106, CD184, CD271, and CD325. Cell doubling time was 29.6 ± 1.3 h. The cells were capable of directed osteogenic, adipogenic and chondrogenic differentiation. The cells showed 35.7% colony forming efficiency and a tendency to 3D spheroid formation. The GTG-banding assay confirmed the stability of eMSC karyotype during long-term culturing (up to P8). After 48 h incubation period in serum-free medium eMSC secreted anti-inflammatory IL-1ra, as well as IL-6, IL-8 and IFNγ, angiogenic factors VEGF, GM-CSF and FGF-2, chemokines IP-10 and MCP-1. Conclusion: Thus, cultured endometrial stromal cells meet minimal ISCT criteria for MSC. Proliferative potential, karyotype stability, multilineage plasticity and secretome profile make eMSC an attractive object for the regenerative medicine use Доп.точки доступа: Zlatska, A. V. Rodnichenko, A. E. Gubar, O. S. Zubov, D. O. Novikova, S. N. Vasyliev, R. G. Свободных экз. нет |